| rhizopodin, a new compound from myxococcus stipitatus (myxobacteria) causes formation of rhizopodia-like structures in animal cell cultures. production, isolation, physico-chemical and biological properties. | a new cytostatic compound, rhizopodin, was isolated from the culture broth of the myxobacterium, myxococcus stipitatus. the compound inhibited growth of various animal cell cultures without killing the cells. the id50, measured by an mtt assay, was 12 approximately 30 ng/ml, depending on the cell line. especially cells growing fibroblast-like showed typical morphological changes. they became larger and within hour formed long branching and reticular runners. these morphological changes were irre ... | 1993 | 8514628 |
| melithiazols, new beta-methoxyacrylate inhibitors of the respiratory chain isolated from myxobacteria. production, isolation, physico-chemical and biological properties. | new antibiotic compounds, melithiazols, were isolated from the culture broth of strains of the myxobacteria melittangium lichenicola, archangium gephyra, and myxococcus stipitatus. the compounds belong to the group of beta-methoxyacrylate (moa) inhibitors and are related to the myxothiazols. the melithiazols show high antifungal activity, but are less toxic than myxothiazol a and its methyl ester in a growth inhibition assay with mouse cell cultures. the melithiazols inhibit nadh oxidation by su ... | 1999 | 10580385 |
| configurational assignment of rhizopodin, an actin-binding macrolide from the myxobacterium myxococcus stipitatus. | the relative and absolute stereochemistry of the 38-membered myxobacterial polyketide rhizopodin, a potent actin-binding macrolide, was determined by j-based configurational analysis in combination with molecular modeling and chemical derivatization. | 2008 | 18956059 |
| emended descriptions of the genera myxococcus and corallococcus, typification of the species myxococcus stipitatus and myxococcus macrosporus and a proposal that they be represented by neotype strains. request for an opinion. | the genus corallococcus was separated from the genus myxococcus mainly on the basis of differences in morphology and consistency of swarms and fruiting bodies of the respective members. phylogenetic, chemotaxonomic and physiological evidence is presented here that underpins the separate status of these phylogenetically neighbouring genera. emended descriptions of the two genera are presented. the data also suggest that the species corallococcus macrosporus belongs to the genus myxococcus. to the ... | 2009 | 19567579 |
| complete genome sequence of myxococcus stipitatus strain dsm 14675, a fruiting myxobacterium. | hallmarks of the myxobacteria include the formation of spore-filled fruiting bodies in response to starvation and synthesis of secondary metabolites. myxococcus stipitatus forms morphologically highly distinct fruiting bodies and produces secondary metabolites with antibiotic or cytotoxic activities. here, we present the 10.35-mb genome sequence of m. stipitatus strain dsm 14675. | 2013 | 23516218 |
| identification of the phenalamide biosynthetic gene cluster in myxococcus stipitatus dsm 14675. | phenalamide is a bioactive secondary metabolite produced by myxococcus stipitatus. we identified a 56 kb phenalamide biosynthetic gene cluster from m. stipitatus dsm 14675 by genomic sequence analysis and mutational analysis. the cluster is comprised of 12 genes (mysti_04318-mysti_04329) encoding three pyruvate dehydrogenase subunits, eight polyketide synthase modules, a non-ribosomal peptide synthase module, a hypothetical protein, and a putative flavin adenine dinucleotide-binding protein. dis ... | 2016 | 27291672 |
| two new ring-contracted congeners of rhizopodin illustrate significance of the ring moiety of macrolide toxins on the actin disassembly-mediated cytotoxicity. | two new cytotoxic dilactones, bisisorhizopodin (1) and isorhizopodin (2), together with known divalent actin depolymerizer rhizopodin (3), were isolated from the culture broth of a myxobacterium myxococcus stipitatus. spectroscopic analyses established that 1 and 2 are doubly and singly acyl-migrated isomers of 3, respectively, and comparison of their cytotoxicity revealed gradual decrease in the activity as the size of the ring contracted. because the side chains of macrolide toxins uniformly b ... | 2014 | 24583785 |