| mycobactericidal activity of glutaraldehyde solutions. | aqueous solutions of alkaline glutaraldehyde (buffered at ph 8.5) inactivated a standard suspension of mycobacterium tuberculosis h37rv faster than the corresponding acid (ph 3.7 preparation. quantitative differences in the rate of inactivation of eight other species of mycobacterium were determined using a 1% solution of alkaline glutaraldehyde and inactivation of residual glutaraldehyde with 1% sodium bisulfite solution. variations in the rate of kill were observed between the various mycobact ... | 1976 | 11227 |
| demonstration & purification of three fatty acid synthetases from mycobacterium tuberculosis h37rv. | | 1979 | 43835 |
| cross-resistant relationships among the aminoglucoside antibiotics in mycobacterium tuberculosis. | phenotypes of isolates of mycobacterium tuberculosis h37rv showing resistance to the aminoglucoside antibiotics streptomycin, viomycin, kanamycin, capreomycin, tuberactinomycin n, lividomycin and paromomycin could be grouped into the following types: (i) resistant only to different levels of streptomycins; (2) resistant only to a low level of kanamycin; (3) triply resistant, to low levels of viomycin, tuberactinomycin n and capreomycin; (4) triply resistant, to a low level of kanamycin and high ... | 1975 | 50402 |
| acid-fastness of mycobacteri,m tuberculosis h37rv following infection by mycobacteriophage ds6a. | mycobacterium tuberculosis h37rv demonstrates a loss of acid-fastness following exposure to specific mycobacteriophage ds6a. no effect was seen with another mycobacteriophage gs7 which does not lyse this organism. | 1976 | 62438 |
| [experiments for the serological establishment of the species of mycobacteria inducing an immune restructuring of the macroorganism]. | serological investigations are carried out on hyperimmune rabbit and guinea pig sera with two antigens each of mycobacterium tuberculosis h37rv, mycobacterium bovinus, mycobacterium avium and mycobacterium kanzassi. one of the antigens is obtained through electrohydraulic destruction of delipidated mycobacteria, while the other constitutes cell walls, purified with ribonuclease, of non-delipidated mycobacterial cells, destroyed by the same method. the antigens, prepared by the electrohydraulic m ... | 1978 | 87060 |
| comparison of several culture media used for studies on mycobacteriophages. | the value of rva, n-1, 7h10, 7h11 and sauton's media for studies on mycobacteriophage infeciton and lysis of mycobacteria was assessed. experiments were made with mycobacteriophages bgi, bki, cri-3, g37, and lg, all of which lyse mycobacterium smegmatis strain 607b, and with mycobacteriophage ds6a which lyses mycobacterium tuberculosis strain h37rv. the methods involved "direct lysis", the measurement of "routine test dilutions" and counts of plaque-forming units. it was found that n-1, 7h10 and ... | 1978 | 96257 |
| tryptophan uptake by mycobacterium tuberculosis h37rv: effect of rifampin and ethambutol. | uptake of radioactive tryptophan by mycobacterium tuberculosis h(37)rv grown in vitro and in vivo was investigated. k(m) values indicated low affinity, and sodium azide inhibited uptake. rifampin at the minimal inhibitory concentration had no effect, whereas ethambutol inhibited uptake only in the bacilli grown under in vitro conditions. the significance of these results is discussed. | 1978 | 96733 |
| studies on transfer rna from mycobacteria. | active preparations of trna and aminoacyl-trna synthetases have been isolated from exponentially growing cells of mycobacterium smegmatis and mycobacterium tuberculosis h37rv. though the aminoacyl-trna synthetases of older cells retain their activity, the trnas seem to undergo modification and show poorer activity. the mycobacterial enzyme preparations catalyse homologous and heterologous aminoacylation between trna from the two species (m. smegmatis and m. tuberculosis h37rv) or from escherichi ... | 1978 | 96929 |
| mycolic acids. a reinvestigation. | mycolic acids derived from the cell walls of mycobacterium bovis bcg, mycobacterium bovis bovinus i, mycobacterium smegmatis, and mycobacterium tuberculosis h37rv have been fractionated as their p-bromophenacyl esters by a two-step high performance liquid chromatographic procedure: 1) adsorption chromatography on 10-micrometer particle size silica gel, and 2) reverse phase partition chromatography on a 10-micrometer particle size support containing a c18 bonded phase. this procedure has resulted ... | 1978 | 97301 |
| effects of panax ginseng extracts on the growth of mycobacterium tuberculosis h37rv. | | 1979 | 113372 |
| pathways of carbohydrate metabolism in mycobacterium tuberculosis h37rv1. | radiorespirometric studies using glucose labelled at 1, 2, 3-4, and 6 positions and enzymatic studies were conducted to determine the primary pathways of glucose dissimilation in mycobacterium tuberculosis h37rv. the pattern of 14co2 recovery was c3-4 greater than c1 greater than c6 = c2. the embden-meyerhof pathway was found to be the predominant pathway for glucose oxidation, operative to the extent of 94%. the pentose phosphate pathway accounted for the remaining 6%. maximum incorporation of ... | 1975 | 172204 |
| the relationship between nicotinamide adenine dinucleotide concentration and antibacterial activity of isoniazid in mycobacterium tuberculosis. | the relationship between the antibacterial effect of isoniazid and the intracellular concentration of nicotinamide adenine dinucleotide (nad) was investigated in mycobacterium tuberculosis strain h37rv given continuous and pulsed exposures to the drug. depletion of nad to a plateau value occurred rapidly during exposure, and recovery after a pulse of isoniazid was also rapid. it seemed unlikely that nad depletion was the direct cause of the antibacterial activity because (1) insufficient depleti ... | 1977 | 192114 |
| variations in the pathways of malate oxidation and phosphorylation in different species of mycobacteria. | mycobacterium tuberculosis h37rv, the slow-growing human pathogenic strain of tubercle bacilli and mycobacterium smegmatis and mycobacterium phlei, the fast-growing saprophytes, have shown variations regarding the type of dehydrogenase that initiates malate oxidation in the respiratory chain. m. tuberculosis h37rv is characterized by having a malate oxidase system (designated malnad pathway) in which malate oxidation is mediated by the nad+-dependent malate dehydrogenase (ec 1.1.1.37) but not by ... | 1975 | 234747 |
| the purification and properties of peroxidase in mycobacterium tuberculosis h37rv and its possible role in the mechanism of action of isonicotinic acid hydrazide. | peroxidase from mycobacterium tuberculosis h37rv was purified to homogeneity. the homogeneous protein exhibits catalase and y (youatt's)-enzyme activities in addition to peroxidase activity. further confirmation that the three activities are due to a single enzyme was accomplished by other criteria, such as differential thermal inactivation, sensitivity to different inhibitors, and co-purification. the y enzyme (peroxidase) was separated from nadase (nad+ glycohydrolase) inhibitor by gel filtrat ... | 1975 | 242321 |
| rate of ribonucleic acid chain growth in mycobacterium tuberculosis h37rv. | two methods were employed to measure the rate of ribonucleic acid (rna) chain growth in vivo in mycobacterium tuberculosis h37rv cultures growing in sauton medium at 37 degrees c, with a generation time of 10 h. in the first, the bacteria were allowed to assimilate [3h]uracil or [3h]guanine into their rna for short time periods. the rna was then extracted and hydrolyzed with alkali, and the radioactivity in the resulting nucleotides and nucleosides was measured. the data obtained by this method ... | 1977 | 402354 |
| [an immunological enhancement phenomenon in different experimental bacterial infections]. | to demonstrate that an enhancement phenomenon might be involved during the development of bacterial infections, one has to establish that specific humoral antibodies protect, in some way, microorganisms against the defence reations of the host. this report presents the evidence that specific antibodies could be evolved in assuming a certain survival of bacterial cells in host's tissue. (1) homologues antistaphylococcal sera passively potentiated the development of experimental staphylococcal syn ... | 1977 | 403848 |
| loss of hextuple resistance to aminoglycoside antibiotics in mycobacterium tuberculosis (h37rv) by mutation to isoniazid resistance and by incubation at high temperature. | | 1977 | 404392 |
| tryptophan uptake by mycobacterium tuberculosis h37rv--inhibition by isoniazid. | | 1977 | 410415 |
| antitubercular 2,8-bis(alkylaminomethyl)phenazines. | the preparation and antitubercular properties of a series of 2,8-bis(alkylaminomethyl)phenazines are described. these compounds all inhibited the growth of mycobacterium smegmatis atcc 607 in vitro. 2,8-bis(dibutylaminomethyl)phenazine (5c) was also active against a lethal mycobacterium tuberculosis h37rv infection in mice. | 1978 | 650670 |
| transfer of adoptive immunity to tuberculosis in mice. | a system is described for studying adoptive immunity to tuberculosis in syngeneic mice. donor mice were immunized with 10(4) bcg intravenously, and lymphoid cells were harvested 28 days later. adoptive immunity was measured in recipient mice in terms of the inhibition of growth of bcg in the liver and spleen following intravenous injection. adoptive immunity was expressed optimally when recipients were sublethally irradiated (500 r), challenged with 10(4) to 10(5) viable organisms, and given sen ... | 1975 | 806520 |
| inactivation of phages ds6a and d29 by acetone extracts of mycobacterium tuberculosis and mycobacterium bovis. | the adsorption rate constant for mycobacteriophage ds6a on clumped cells of mycobacterium tuberculosis strain h37rv was found to be 6.33 x 10(-13) ml per min per colony former. acetone-extracted cells of h37rv did not absorb phage ds6a. acetone extracts of cells of h37rv and of mycobacterium bovis strain bcg inhibited plaque formation of mycobacteriophage ds6a and of phage d29. suspensions of these extracts in heart infusion broth with 0.002 m calcium chloride and 1 per cent dioxane required 20 ... | 1975 | 807139 |
| experimental male genital tuberculosis-appraisal of protective value of bcg vaccination. | as part of an experimental study of the spread of urogenital tuberculosis, the development of tuberculous infection was compared in vaccinated and non-vaccinated guinea pigs after challenge with mycobacterium tuberculosis strain h37rv into the seminal vesicle. the animals were saccrified 35 days after challenge. morphological observations indicate that tuberculous infection in an immunized animal is retarded, but spreads by the same routes as in an nonimmunized animal. on microbial enumeration s ... | 1975 | 808092 |
| host-parasite relationships in experimental airborne tuberculosis. v. lack of hematogenous dissemination of mycobacterium tuberculosis to the lungs in animals vaccinated with bacille calmette-guérin. | the influence of bacille calmette-guérin (bcg) on the pathogenesis of experimental airborne tuberculosis was studied. in a model that approximates the conditions under which man is vaccinated and infected, bcg-vaccinated and unvaccinated guinea pigs were infected by the respiratory route with an inoculum that resulted in the inhalation and retention (by each animal) of approximately three virulent tubercle bacilli (mycobacterium tuberculosis strain h37rv). hematogenous seeding of the lungs occur ... | 1976 | 812927 |
| fructose-1,6-diphosphate aldolase of mycobacterium tuberculosis h37rv. | | 1975 | 815159 |
| distribution of mycobacteria grown in vivo in the organs of intravenously infected mice. | suspensions of 35-day-old mycobacterium tuberculosis h37rv prepared from stirred liquid cultures and injected intravenously into cd-1 mice accumulated in the lungs at a significantly higher concentration that that seen with logarithmically growing cells. mice were infected with logarithmic 8-day-old or stationary phase 35-day-old suspensions of h37rv, and 24 hours later, the bacilli within pooled lung and splenic homogenates were recovered by differential centrifugation. the bacilli were then ho ... | 1976 | 816236 |
| purification and properties of dna-dependent rna polymerase from mycobacterium tuberculosis h37rv. | rna polymerase (nucleosidetriphosphate: rna nucleotidyltransferase dna-dependent), ec 2.7.7.6) was purified approximately 200 fold from mycobacterium tuberculosis h37rv cells. the purified enzyme has a molecular weight of about 330 000-350 000 and is composed of four subunits. the subunits beta', beta and sigma have molecular weights different from those of escherichia coli polymerase; the fourth, alpha subunit has a similar weight. the purified enzyme is a thousand-fold more sensitive to rifamp ... | 1976 | 816387 |
| enhancement activity of anti-mycobacterial sera in experimental mycobacterium bovis (bcg) infection in mice. | the passive transfer of rabbit anti-mycobacterial immunoglobulins was directed against either living or soluble extracts of mycobacterium tuberculosis strain h37rv, which promotes the multiplication of the bcg strain of m. tuberculosis in the spleen of mice infected with low doses of this latter strain. this enhancing effect was reduced significantly when antisera were absorbed with living bcg. moreover, such treatment led to the removal of all hemagglutinating antibodies when antisera were test ... | 1976 | 818022 |
| sulfatides of mycobacterium tuberculosis: the structure of the principal sulfatide (sl-i). | the gross structural features of five families of multiacylated trehalose 2-sulfates elaborated by mycobacterium tuberculosis strain h37rv are described. the principal sufatide sl-i is a 2,3,6,6'-tetraacyl-alpha,alphs'-d-trehalose 2'-sulfate, whose component carboxylate substituents (and homolgy) were previously established. in the present study the specific locations of the acyl substituents were assigned. the desulfated glycolipid (sl-i-cf) was methanolyzed on a column of diethylaminoethylcell ... | 1976 | 820371 |
| synthesis and biological activity of some new furan quaternary salts. | a series of new n-(5-substituted 2-furfuryl)-n,n-dimethyl-n-aryloxyalkyl quaternary ammonium salts relating to general structure iv has been synthesized by reacting 5-substituted 2-(n,n-dimethylaminomethyl)furans iia-d with appropriate aryloxyalkyl bromides iii. the resulting compounds are tested for in vitro antimicrobial activity. a simpler synthesis of 5-nitro-2-(n,n-dimethylaminomethyl)furan (iid) involving the reduction of n,n-dimethyl-5-nitro-2-furamide (ib) with diborane is described. a n ... | 1977 | 833814 |
| antimicrobial agents: synthesis and antimicrobial activity of new aryloxyalkyl esters of p-hydroxybenzoic acid. | several new aryloxyalkyl esters of p-hydroxybenzoic acid were synthesized and screened for in vitro antimicrobial activity. although a few compounds showed low antifungal activity, many possessed appreciable in vitro antibacterial activity. however, none of these compounds was active against mycobacterium tuberculosis (h37rv). | 1976 | 957118 |
| [diagnostic role of fiberoptic bronchoscopy for mycobacterial diseases--serological diagnosis]. | detection of igg antibodies against purified cord factor (trehalose-6, 6'-dimycolate) prepared from mycobacterium tuberculosis h37rv was carried out by the method of enzyme-linked immunosorbent assay (elisa) and its diagnostic usefulness was also evaluated in this study. sera from 65 patients with active pulmonary tuberculosis, 58 patients with inactive pulmonary tuberculosis, 36 patients with diseases other than tuberculosis and 66 healthy adults were examined. patients with active pulmonary tu ... | 1992 | 1294783 |
| the mycobacterium tuberculosis 38-kda antigen: overproduction in escherichia coli, purification and characterization. | the 38-kda protein (ag38) of the gram+ bacterium, mycobacterium tuberculosis h37rv, is an immunodominant antigen of potential utility for diagnosis and vaccine development. assessment of this potential requires large amounts of the purified protein that would be difficult, if not impossible, to obtain from m. tuberculosis itself. the gene coding for ag38 had been previously cloned and in the present study was expressed as an unfused protein in escherichia coli under the control of strong transcr ... | 1992 | 1379565 |
| the nucleotide sequence of the promoter, 16s rrna and spacer region of the ribosomal rna operon of mycobacterium tuberculosis and comparison with mycobacterium leprae precursor rrna. | mycobacterium tuberculosis h37rv has a single rrn (ribosomal rna) operon. the operon was cloned and a region of 1536 nucleotides was sequenced, starting 621 bp upstream from the 5'-end of the 16s rrna coding region and continuing to the start of the 23s rrna coding region. the 16s rrna sequence inferred from the gene sequence was found to differ in one position from mycobacterium bovis (nucleotide 1443) and from mycobacterium microti (nucleotide 427). a single putative promoter was identified on ... | 1992 | 1382114 |
| structural elucidation of a novel family of acyltrehaloses from mycobacterium tuberculosis. | analysis of the lipids of mycobacterium tuberculosis h37rv, by both normal- and reverse-phase thin-layer chromatography, revealed a series of novel glycolipids based on 2,3-di-o-acyltrehalose. the structures of these acylated trehaloses were elucidated by a combination of gas chromatography-mass spectrometry, 1h, 13c, two-dimensional 1h-1h, and 1h-13c nuclear magnetic resonance spectrometry. the fatty acyl substituents were mainly of three types: saturated straight-chain c16-c19 acids; c21-c25 " ... | 1992 | 1390757 |
| antitubercular activity of trifluoperazine, a calmodulin antagonist. | trifluoperazine, a calmodulin antagonist, completely inhibited the growth of mycobacteria. the minimum inhibitory concentrations in shake cultures in a synthetic medium containing 0.2% tween 80 were 5 and 8 micrograms/ml, respectively, for the human pathogenic strain mycobacterium tuberculosis h37rv and m. tuberculosis resistant to isoniazid. when added to a growing culture of m. tuberculosis h37rv on the 10th day (mid exponential phase), trifluoperazine 50 micrograms/ml further arrested growth ... | 1992 | 1427006 |
| the pattern of mycobacterial antigen recognition in sera from mantoux-negative individuals is essentially unaffected by bacille calmette-guérin (bcg) vaccination in either south india or london. | paired sera were obtained before and 8 weeks after routine bcg vaccination from 20 ppd-s mantoux-negative individuals who were living adjacent to the chingleput bcg vaccine trial area in tamil nadu and from seven mantoux-negative school-children in london, uk. most subjects became mantoux-positive after vaccination. in elisa tests against soluble extracts of bcg or mycobacterium tuberculosis h37rv or against ppd-s, pre-vaccination antibody titres of south indian subjects were about twice those o ... | 1992 | 1516256 |
| antagonism between isoniazid and the combination pyrazinamide-rifampin against tuberculosis infection in mice. | mice that had been inoculated intravenously with 6.30 log10 mycobacterium tuberculosis h37rv 14 days earlier were administered one of three combinations of drugs, i.e., isoniazid (inh)-rifampin (rmp)-pyrazinamide (pza), inh-rmp, and rmp-pza, during an initial 2-month period to mimic the initial phase of chemotherapy for human tuberculosis and during a later 4-month period to mimic the continuation phase of chemotherapy. at the end of the initial phase, all three combined regimens were found to h ... | 1992 | 1622164 |
| a comparative serological study of antigenic glycolipids from mycobacterium tuberculosis. | two fractions of antigenic diacyl trehaloses (dat1 and dat2) were isolated from the type strain of mycobacterium tuberculosis (h37rv). phenolic glycolipid (pgl) and polar glycolipid antigens (c1-c4) were isolated from an unusual smooth so-called 'canetti' strain of m. tuberculosis. these lipids were analyzed by enzyme-linked immunosorbent assay (elisa) using antisera against a range of mycobacteria and sera from 50 tuberculosis patients and 25 healthy blood donors. all the lipids gave strong rea ... | 1992 | 1643294 |
| enhancement of monocyte antimycobacterial activity by diethyldithiocarbamate (dtc). | diethyldithiocarbamate (dtc) has been recently reported to significantly reduce the incidence of opportunistic infections in hiv-infected patients. the present study addresses the question whether dtc is capable of stimulating antimycobacterial activity of mononuclear phagocytes. we found that peripheral blood mononuclear cells (pbmc) of healthy subjects preincubated in vitro with 100-1000 ng/ml of dtc and thereafter infected with mycobacterium tuberculosis h37rv or mycobacterium avium-intracell ... | 1991 | 1667649 |
| [cellular mechanisms of suppression of t-lymphocyte proliferation by lung cells in experimental tuberculosis]. | the ability of interstitial lung cells from mice, infected with mycobacterium tuberculosis h37rv, to suppress proliferative responses of immune lymphocytes to mycobacterial (ppd) and unrelated (staphylococcus aureus cytoplasm) antigens was studied. two types of suppression were observed: the specific one, which was characteristic of the ppd-response only; and non-specific. the latter was mediated mainly by prostaglandins, since it could be abolished by indomethacin. both types of suppression dep ... | 1990 | 1705456 |
| [specific antibodies to different mycobacterial antigens in patients with pulmonary tuberculosis]. | antibodies to three mycobacterial antigens, soviet tuberculin and ultrasound-treated mycobacterium tuberculosis (h37rv) and m. bovis (bcg) were detected by enzyme immunoassay in 90 patients with pulmonary tuberculosis and 75 normal subjects. antibody detection rates and levels were found related to the form of tuberculous process. the detection rate was rather low in focal tuberculosis and virtually the same for all the antigens (15.3 percent). in disseminated processes (infiltrative and particu ... | 1991 | 1715435 |
| concurrent human immunodeficiency virus and mycobacterial infection of macrophages in vitro does not reveal any reciprocal effect. | to test whether in vitro infection of macrophages with either human immunodeficiency virus (hiv) or mycobacteria would influence the replication of the other pathogen, macrophages were infected sequentially with the macrophage-tropic isolate htlv-iiiba-l/85 and mycobacterium tuberculosis h37rv or mycobacterium avium. the intracellular growth of mycobacteria was measured by colony counting and radiometric assay of macrophage lysates and the replication of hiv by the release of p24 antigen into th ... | 1992 | 1727900 |
| resistance to intravenous inoculation of mycobacterium tuberculosis h37rv in mice of different inbred strains following immunization with a leprosy vaccine based on mycobacterium w. | four strains of mice, namely balb/c, c57bl/6 ncrl (bcgs), c3h/he ncrl and cba/n (bcgr) were experimentally infected with mycobacterium tuberculosis h37rv (trudeau institute, saranac lake, ny) to induce sub-lethal infection. the level of infection was assessed by screening tuberculin reaction, pulmonary lesions, and viable units of mycobacteria recovered from the lung, spleen and liver. on prior immunization with 10(7) heat-killed suspension of mycobacterium w, an anti-leprosy vaccine currently u ... | 1991 | 1901186 |
| [in vitro experiment showing that chemotherapy of tuberculosis by aminoglycoside antibiotics may influence successive chemotherapy with rifampicin]. | when mycobacterium tuberculosis strain h37rv was cultivated in ogawa egg medium containing 5 micrograms/ml streptomycin and/or 10 micrograms/ml kanamycin, which were considered as subinhibitory, it was observed that growing bacterial population contained several times more rifampicin-resistant mutants than did the parent strain. the ratio of isoniazid-resistant mutants did not change by the above treatment. the finding suggests that, even without the use of rifampicin, the bacterial population o ... | 1991 | 1901613 |
| human tuberculosis sera show prominent antibody responses to particulate fractions of mycobacterium tuberculosis. | sera from smear-positive pulmonary tuberculosis patients and normal control subjects in madras were analyzed by western blotting for their reactivity with soluble and particulate (membrane-rich and cell wall-rich) antigen fractions extracted by sonication from mycobacterium tuberculosis h37rv. discrimination between patient and control sera was best with particulate antigen fractions: 60% of patient sera reacted with a 38-kd antigen band and 90% reacted with a 55-kd band. reactions of control se ... | 1991 | 1905304 |
| rapid serodiagnosis of human mycobacteriosis by elisa using cord factor (trehalose-6,6'-dimycolate) purified from mycobacterium tuberculosis as antigen. | igg antibodies against purified cord factor (trehalose-6,6'-dimycolate, tdm) in sera of 99 patients infected with mycobacteria (42 patients with tuberculosis excreting tubercle bacilli in the sputum, 11 patients with non-tuberculous mycobacteriosis excreting acid-fast bacilli in the sputum, and 46 patients without bacilli in the sputum but diagnosed as having pulmonary tuberculosis by chest x-ray films and physical examination), five patients with lung cancer, and 100 healthy controls which incl ... | 1991 | 1931132 |
| efficacy of intermittent pyrazinamide in experimental murine tuberculosis. | cflp mice were infected intravenously with mycobacterium tuberculosis strain h37rv and the progress of chemotherapy was followed by counts of viable bacilli in the lung and spleen. after spleen counts had reached log10 7.0, 12 experimental groups, each containing 10 mice, were treated for 8 weeks with pyrazinamide (pza) given in mean daily dosages of 100, 200 or 400 mg/kg/day, with the interval between the doses within each dosage group being 1, 2, 4 or 8 days. all mice were also given 25 mg iso ... | 1991 | 1949213 |
| quantitative and qualitative studies on the major extracellular antigen of mycobacterium tuberculosis h37rv and mycobacterium bovis bcg. | the mycobacterium tuberculosis antigen 85 is a biologically important antigen. tuberculosis patients may have strong antibodies against it, and their peripheral blood mononuclear cells respond to it with gamma-interferon production and lymphocyte proliferation. antigen 85 is actively secreted into the culture medium during culture in vitro and is known to bind human fibronectin. a double-antibody enzyme-linked immunosorbent assay (elisa) for quantification of antigen 85 is described. a mouse mon ... | 1990 | 2109556 |
| [use of elisa for the detection of igg antibodies to mycobacterium tuberculosis in the diagnosis of tuberculosis]. | the presence of mycobacterial antibodies of igg type was determined in serum, cerebrospinal fluid and pleural puncture fluid by using elisa and soluble antigens of the strain mycobacterium tuberculosis h37rv. in patients suffering from tuberculosis the most frequently found titer was 1:320 and above. this titer was however found also in 30% of healthy subjects of the control group and 29% of patients with other than tuberculous disease. the sensitivity of the test was 0.7692 and its specificity ... | 1990 | 2110495 |
| intracellular tubercle bacilli-alveolar macrophage lysosomal enzymes interaction in experimental tuberculosis. | this study is an attempt to understand the mechanism of macrophage activation and its effect on the microbicidal properties of the macrophage. alveolar macrophages (am) from normal and bcg-vaccinated guinea pigs were harvested at intervals of 1, 7, 14, 21, and 28 days. half of the guinea pigs from each group were challenged intratracheally with mycobacterium tuberculosis h37rv. in am, the levels of three lysosomal enzymes, beta-galactosidase (beta-gal), n-acetylglucosaminidase (n-ac), and lysozy ... | 1990 | 2116247 |
| detection of antituberculous activity in plant extracts. | ten of 408 ethanolic extracts of plants inhibited growth of mycobacterium tuberculosis h37rv at dilutions of 1 in 160 to 1280 and a high proportion of the other extracts inhibited growth at lower dilutions. some of the plants, after purification, may be therapeutically useful. in view of the environmental threat to many plant species, screening of such plants for potential therapeutic properties, including antituberculous and other antimicrobial activities, are urgently required before they are ... | 1990 | 2118130 |
| intracellular bactericidal activity of ciprofloxacin and ofloxacin against mycobacterium tuberculosis h37rv multiplying in the j-774 macrophage cell line. | among new fluoroquinolone derivatives, the n-cyclopropylanalogs ciprofloxacin (cipro) and ofloxacin (oflo) have recently been found to have a high in vitro activity against a variety of mycobacteria (2). in this study, we have investigated their action against intracellularly growing mycobacterium tuberculosis. for this purpose, the j-774 macrophage cell line was infected with the h37rv type strain of m. tuberculosis, and drugs (at their serum level concentrations obtainable in healthy individua ... | 1990 | 2119180 |
| natural killer cell activity in patients with pulmonary tuberculosis and in healthy controls. | natural killer (nk) cell activity of freshly isolated peripheral blood mononuclear cells (mnc) or cells stimulated for 72 h with 10 micrograms/ml of a sonicate antigen of mycobacterium tuberculosis h37rv were studied in healthy responder and non-responder controls, as detected by lymphocyte proliferation with specific antigen, and in patients with pulmonary tuberculosis. k-652 cells were used as targets in a 4 h 51cr release assay. mnc from patients exhibited a significant decrease in nk functio ... | 1990 | 2120829 |
| tuberculostatic activity of henna (lawsonia inermis linn.). | the tuberculostatic activity of the herb henna (lawsonia inermis linn.) was tested in vitro and in vivo. on lowenstein jensen medium, the growth of tubercle bacilli from sputum and of mycobacterium tuberculosis h37rv was inhibited by 6 micrograms/ml of the herb. in vivo studies on guinea pigs and mice showed that the herb at a dose of 5 mg/kg body weight led to significant resolution of experimental tuberculosis following infection with m. tuberculosis h37rv. | 1990 | 2125156 |
| [the genetic regulation of host response to inoculation with mycobacterium bovis (bcg) and mycobacterium tuberculosis h37rv]. | vaccination with m. bovis (bcg) essentially prolonged survival time (st) of several strain mice, with the exception, of cba/n, infected with m. tuberculosis h37rv. st of cba/n, differing from cba by xid mutation, was not prolonged by vaccination. mouse strains with alternative alleles of bcg gene (s and r) and fzy gene as a genetic marker for bcg5 were used for segregation analysis. it was shown that st, the level of dth reaction of mice infected with m. tuberculosis h37rv, and protective effect ... | 1990 | 2128037 |
| [purified protein derivative antigen purified by using a monoclonal antibody to mycobacterium tuberculosis]. | a component of mycobacterium tuberculosis h37rv ppd referred as c3ag was purified with a column of affinity chromatography to which a monoclonal antibody (tb-15c3) was conjugated. c3ag was found to have two bands in 56,000 and 66,000 by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. western blot revealed that both fragments were recognized by tb-15c3. by elisa c3ag was shown to react with rabbit antisera against m. tuberculosis, m. kansasii, m. screfulaceum, m. bovis bcg, but not to ... | 1990 | 2128213 |
| [suppression of immune response by lung cells in experimental tuberculosis]. | in vitro proliferative response of lung cells from mice infected with mycobacterium tuberculosis h37rv against ppd and con a was studied. it was shown that the infected lung contained immune t cells, but their response in vitro was totally inhibited by plastic and nylon wool adherent suppressor cells. the whole population of lung cells from infected, but not intact mice, efficiently suppressed the proliferative response of immune lymph node cells against various antigens (non-specific suppressio ... | 1990 | 2145988 |
| monoclonal antibodies produced in balb.b10 mice define new antigenic determinants in culture filtrate preparations of mycobacterium tuberculosis. | a panel of monoclonal antibodies were derived from balb.b10 mice immunized with a culture filtrate from mycobacterium tuberculosis h37rv. of these antibodies, 10 were examined more closely for antigen specificity and interspecies reactivity. six antibodies were used as immunosorbents for affinity purification of their corresponding antigens. two monoclonal antibodies (hbt 2 and hbt 11) reacted with a 17-kilodalton antigen, and a competition assay showed that these antibodies are directed against ... | 1988 | 2466047 |
| evidence for a highly conserved, immunoreactive lipid in mycobacterium tuberculosis. | a survey of the fractionated lipids of mycobacterium tuberculosis h37rv was conducted using rabbit antiserum raised to homologous and heterologous mycobacteria. one minor, highly apolar lipid was resolved by florisil column chromatography, which reacted preferentially to anti-m. tuberculosis h37rv rabbit antibodies. other chromatographic properties, i.e., thin-layer chromatographic mobility and staining properties, suggested an analog of the phenolic glycolipid of mycobacterium leprae. prelimina ... | 1989 | 2466430 |
| comparative studies of antigenic glycolipids of mycobacteria related to the leprosy bacillus. | the leprosy bacillus, mycobacterium leprae, is a member of a small group of mycobacteria comprising the species mycobacterium bovis, mycobacterium marinum, mycobacterium kansasii, mycobacterium tuberculosis, mycobacterium ulcerans and related taxa. this relationship is based on the similarity of the characteristic lipid types in the cell envelope. mycobacterium leprae produces a phenolic glycolipid antigen which is species specific. this communication reports a comparison of the specificity of t ... | 1989 | 2504005 |
| synthesis and antitubercular activity of n-(2-naphthyl)glycine hydrazide analogues. | n-(2-naphthyl)glycine hydrazide analogues were synthesized and tested for possible in vitro antitubercular activity. n-(2-naphthyl)alanine hydrazide (3), n-methyl-n-(2-naphthyl)glycine hydrazide (5), n-(6-methoxy-2-naphthyl)glycine hydrazide (7), and 3-(2-naphthylamino)butyric acid hydrazide (23) showed potent inhibitory action against mycobacterium tuberculosis h37rv in youman's medium at concentrations ranging from 0.5 to 10.0 micrograms/ml. these compounds showed significant inhibitory action ... | 1989 | 2509708 |
| growth of m. tuberculosis h37rv strain in 7h12 middlebrook liquid medium in the presence of acridine orange and tha. | the influence of acridine orange and tha on the growth of mycobacterium tuberculosis h37rv strain in 7h12 middlebrook liquid medium was studied. as compared to acridine orange, tha also inhibited the growth of the h37rv mycobacterium tuberculosis strain but at much higher concentrations. | 1989 | 2554470 |
| biological and immunological properties of avirulent strain of listeria innocua. | the strain isolated by dr. j. h. welshimer from plants has antigenic formula v (vi) ix; xv; xi; ab, c--serovar 6a, is non-haemolytic, produces lipase, and toxic factor ei, is avirulent for adult mice, but causes encephalitis in sucklings. in organs of intravenously injected mice the strain persists and multiplies for 1-3 weeks. the protective effect against listerial infections in mice of this strain administered 2-14 days before challenge is dose depending. after 3 weeks induces resistance of g ... | 1989 | 2631501 |
| serodiagnostic potentialities of enzyme-linked immunosorbent assay (elisa) using mannophosphoinositides of mycobacterium tuberculosis h37rv. | the serological response to mannophosphoinositides of mycobacterium tuberculosis h37rv and to tuberculin-purified protein derivative (ppd) was examined by enzyme-linked immunosorbent assay (elisa) in patients suffering from tuberculosis and related diseases. in sputum positive cases 94% samples were found to be positive to mannoside antigens and 77% to ppd, while in sputum negative cases, 71% of samples gave a positive reaction to mannosides and 54% to ppd. the high specificity of mannoside elis ... | 1988 | 2845238 |
| restriction endonuclease analysis of total deoxyribonucleic acid of mycobacterium tuberculosis h37rv (atcc 27294) and of m. bovis (atcc 19210). | total dna from two slowly-growing pathogenic mycobacterial species propagated in vitro was isolated, digested with each of 34 restriction endonucleases and analyzed by agarose gel electrophoresis. the most distinct profiles for m. tuberculosis (atcc 27294) and for m. bovis (atcc 19210) were obtained respectively using (bamhi, drai, clai, ecori, ecorv, hindiii, hpai, sali, smai, xbai, and xmai). the patterns produced for these strains were reproducible and distinguishable from each other. however ... | 1988 | 2854960 |
| deoxyribonucleic acid replication time in mycobacterium tuberculosis h37 rv. | the dna increment method, designed for measuring the increment in the amount of dna after inhibition of initiation of fresh rounds of replication initiation was employed to measure the rate of deoxyribonucleic acid (dna) chain growth in mycobacterium tuberculosis h37rv growing in youman and karlson's medium at 37 degrees c with a generation time of 24 h and also in relatively fast growing species like mycobacterium smegmatis and escherichia coli. from the results obtained, the time required for ... | 1986 | 3087325 |
| isolation and characterization of new glycolipids from mycobacterium tuberculosis h37rv. | | 1986 | 3103042 |
| allergenic and blastogenic reactivity of three antigens from mycobacterium tuberculosis in sensitized guinea pigs. | three antigens from a culture filtrate of mycobacterium tuberculosis h37rv were purified by affinity chromatography, using monoclonal antibodies. the molecular weights of the purified antigens are 17,000 to 19,000, 32,000 to 33,000, and 39,000, respectively, and by their migration patterns in sodium dodecyl sulfate-polyacrylamide gel electrophoresis under reducing and nonreducing conditions, they all appeared to be single-chain polypeptides. western blot and enzyme-linked immunosorbent assay ana ... | 1987 | 3119491 |
| immunological activity of a 14-kilodalton recombinant protein of mycobacterium tuberculosis h37rv. | a 14-kilodalton peptide antigen from mycobacterium tuberculosis was isolated from an escherichia coli lambda gt 11 recombinant dna clone and was identified by western blotting (immunoblotting) with monoclonal antibody tb68. immunization of mice and guinea pigs with the recombinant peptide (rtb68) induced in vitro lymphoproliferative responses in draining lymph node lymphocyte cultures as well as in vivo delayed-type hypersensitivity reactions. moreover, rtb68 was found both to induce and to cros ... | 1987 | 3119499 |
| relationship between the susceptibility of various bacteria to active oxygen species and to intracellular killing by macrophages. | the susceptibilities of six micro-organisms to active oxygen species generated in the xanthine oxidase-mediated bactericidal system were as follows: escherichia coli 81 greater than or equal to listeria monocytogenes egd greater than or equal to salmonella typhimurium hkb-1 greater than or equal to staphylococcus aureus smith much greater than mycobacterium tuberculosis h37rv approximately equal to candida albicans nih a207 (the last two organisms were essentially resistant to this system). the ... | 1987 | 3127536 |
| antibody responses against mycobacterium tuberculosis in 11 strains of inbred mice: novel monoclonal antibody specificities generated by fusions, using spleens from balb.b10 and cba/j mice. | eleven strains of inbred mice were immunized with a culture filtrate of mycobacterium tuberculosis h37rv, and the quality of the antibody responses was determined by immunoblotting. the quantity of mycobacterial antigen used for each immunization ranged from 6 to 750 micrograms per inoculum. the culture filtrate of m. tuberculosis was separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrotransferred to nitrocellulose filters. immunoblotting results were obtained with ... | 1988 | 3135266 |
| rapid susceptibility testing of mycobacterium tuberculosis by bioluminescence assay of mycobacterial atp. | mycobacterial growth was monitored by bioluminescence assay of mycobacterial atp. cultures of mycobacterium tuberculosis h37rv and of 25 clinical isolates of the same species were exposed to serial dilutions of ethambutol, isoniazid, rifampin, and streptomycin. a suppression of atp, indicating growth inhibition, occurred for susceptible but not resistant strains within 5 to 7 days of incubation. breakpoint concentrations between susceptibility and resistance were determined by comparing these re ... | 1988 | 3142348 |
| the protective and allergenic potency of four bcg substrains in use in china determined in two animal models. | the objective of this study was to compare four bcg substrains from regional centres in china with bcg-copenhagen 1331 (danish strain) for protective and allergenic potencies. the comparisons were made in two different guinea pig models: one, a rational animal model involving aerosol challenge with 5-10 cfu mycobacterium tuberculosis (strain h37rv), and the other an animal model involving subcutaneous challenge with 250 cfu of h37rv. for either model, the degree of protection was judged by quant ... | 1988 | 3257006 |
| [comparative characteristics of the activity of proteolytic enzymes and their inhibitors in the lung tissue of guinea pigs during development of generalized and destructive tuberculosis]. | two series of experiments were carried out using 66 guinea pigs infected with tuberculosis. in the first experiment generalized tuberculosis was stimulated by means of subcutaneous administration of mycobacterium tuberculosis h37rv strain at a dose of 0.0001 mg. in the second experiment destructive form of tuberculosis was developed after intrapulmonary administration of the bacteria (1 mg) followed by vcg vaccination. in both experiments the animals were divided into groups with spontaneous for ... | 1986 | 3535249 |
| effects of protein deprivation and bcg vaccination on alveolar macrophage function in pulmonary tuberculosis. | alveolar macrophages were isolated from lung lavage fluids taken from bcg-vaccinated and nonvaccinated guinea pigs 2 and 4 wk after challenge with virulent mycobacterium tuberculosis h37rv by the respiratory route. animals had been maintained on either purified, protein-adequate (30%) or protein-deficient (10%) isocaloric diets for 6 wk prior to respiratory challenge. protein deficiency was accompanied by loss of dermal tuberculin reactivity, even in animals with extensive tuberculosis. vaccinat ... | 1986 | 3717761 |
| effects of diet and genetics on mycobacterium bovis bcg vaccine efficacy in inbred guinea pigs. | strain 2 and strain 13 guinea pigs were vaccinated with mycobacterium bovis bcg and placed on low-protein or protein-adequate diets. five weeks later all animals were infected by the respiratory route with virulent mycobacterium tuberculosis h37rv organisms. four weeks postchallenge, guinea pigs were skin tested with purified protein derivative and sacrificed. protein deficiency resulted in significant reductions in body weight and thymus weight and in an impairment in the ability to control the ... | 1987 | 3804439 |
| laboratory evaluation of a new long-acting 3-azinomethylrifamycin fce 22250. | fce 22250 (3-(n-piperidinomethylazino)methylrifamycin sv) is a member of the new class of 3-azinomethylrifamycins characterized by a long persistance in animals, a good oral absorption and a broad antibacterial spectrum including mycobacteria. in the experimental mice infection sustained by mycobacterium tuberculosis h37rv, fce 22250 shows an efficacy 14 times higher than rifampicin and is still therapeutic when administered once every three weeks. | 1985 | 3839503 |
| antituberculosis activity of ofloxacin (dl 8280) on experimental tuberculosis in mice. | ofloxacin (dl 8280) is rapidly eliminated from mouse organs. it disappears quickly from the lungs and the spleen and more slowly from the liver and the kidneys. the antituberculosis activity of this agent was shown in mice when the agent was given intraperitoneally at daily doses of 1 mg per mouse (approximately 42 mg/kg). the number of viable bacteria of mycobacterium tuberculosis strain h37rv contained in the liver decreased markedly in the mice treated with ofloxacin, and the inhibitory effec ... | 1985 | 3931525 |
| mycobacterium bovis bcg vaccine fails to protect protein-deficient guinea pigs against respiratory challenge with virulent mycobacterium tuberculosis. | specific-pathogen-free hartley guinea pigs were maintained on isocaloric-purified diets either adequate (30%) or moderately deficient (10%) in protein. half of each diet group was vaccinated with viable mycobacterium bovis bcg. six weeks later, all animals were challenged by the respiratory route with virulent mycobacterium tuberculosis h37rv. at intervals of 1, 2, and 3 weeks postchallenge, guinea pigs from each diet and vaccination group were skin tested with tuberculin and sacrificed. protein ... | 1985 | 3932212 |
| synthesis and antibacterial activity of 2,2'-dithiobis(benzamide) derivatives against mycobacterium species. | a series of compounds, which are analogues of 2,2'-dithiobis(benzamide), were synthesized and tested for in vitro antibacterial activity against mycobacterium tuberculosis h37rv including resistant strains against streptomycin, kanamycin, or isonicotinic acid hydrazide. mics of these compounds against atypical mycobacteria, mycobacterium kansasii and mycobacterium intracellulare were also examined. structure-activity relationships were found in a series of (acyloxy)alkyl ester derivatives depend ... | 1985 | 3934384 |
| evaluation of a bioluminescence assay for rapid antimicrobial susceptibility testing of mycobacteria. | the antimicrobial susceptibility of mycobacterium tuberculosis h37rv and 17 clinical isolates of the same species was determined using a bioluminescence assay to measure adenosine triphosphate (atp) produced by bacteria in the broth dilution test performed in dubos-tween-albumin broth. the results were compared with those obtained with the standard dilution proportional method performed on löwenstein-jensen medium. the drugs tested were streptomycin, rifampin, ethambutol and isoniazid. there was ... | 1985 | 3937733 |
| polymorphism of a mycobacterial antigen participating in cell-mediated immunity. | the cross-reactivity of crude sonic extracts of six species of mycobacteria was studied by skin tests in mice and guinea pigs immunised with live mycobacterium tuberculosis strain h37rv, and in patients with pulmonary tuberculosis. all slowly growing mycobacteria elicited a strong delayed hypersensitivity response, m. vaccae a moderate response and m. phlei a poor or no response. a specific target antigen for cell-mediated immunity, known to be present in m. tuberculosis, was also present in all ... | 1985 | 3968706 |
| chemical structure of the antigenic determinants of cell wall polysaccharide of mycobacterium tuberculosis strain h37rv. | | 1971 | 4113500 |
| [change in virulence of the saprophytic strain mycobacterium smegmatitis atcc 607 when mixed with mycobacterium tuberculosis h37rv in the mouse peritoneal cavity]. | | 1972 | 4196872 |
| effect of age on the enzymes of tricarboxylic acid and related cycles in mycobacterium tuberculosis h37rv. | | 1973 | 4200820 |
| protein synthesis in mycobacterium tuberculosis h37rv and the effect of streptomycin in streptomycin-susceptible and -resistant strains. | an efficient in vitro amino acid-incorporating system from mycobacterium tuberculosis h37rv was standardized. ribonucleic acid (rna) isolated from phage-infected m. smegmatis cells served as natural messenger rna and directed the incorporation of (14)c-amino acids into protein. the effects of various antitubercular drugs and "known inhibitors" of protein synthesis on amino acid incorporation were studied. antibiotics like chloramphenicol and tetracycline inhibited mycobacterial protein synthesis ... | 1973 | 4202339 |
| isolation of tuberculin skin test reactive proteins from heated culture filtrate of mycobacterium tuberculosis h37rv. | | 1974 | 4203286 |
| in vitro activity of cephalosporins against mycobacterium tuberculosis h37rv: structure-activity relationships. | | 1973 | 4209052 |
| effect of oxygen tension on the aldolases of mycobacterium tuberculosis h37rv. | | 1974 | 4213059 |
| heat-stable stimulating factor for fatty acid synthetase from mycobacterium tuberculosis h37rv. | | 1974 | 4213136 |
| transduction of a streptomycin r-factor from mycobacterium smegmatis to mycobacterium tuberculosis h37rv. | | 1974 | 4220076 |
| studies on the mechanism of isoniazid resistance in mycobacterium tuberculosis h37rv. | | 1968 | 4239955 |
| vitamin b 12 synthesis by mycobacteria. ii. isoniazid resistance & vitamin b 12 activity of mycobacterium tuberculosis h37rv. | | 1964 | 4243420 |
| polynucleotide phosphorylase of mycobacterium tuberculosis h37rv. | | 1964 | 4243512 |
| nicotinamide-adenine dinucleotide glycohydrolase of mycobacterium tuberculosis h37rv. | | 1964 | 4284486 |
| comparative study of nicotinamide adenine dinucleotide nucleosidase from mycobacterium tuberculosis h37rv & pig brain: effect of isonicotinic acid hydrazide on the enzyme-inhibitor complex. | | 1966 | 4292438 |
| studies on tissue glycohydrolases appearing after infection of guinea pigs with mycobacterium tuberculosis h37rv. | | 1970 | 4393970 |
| response of vaccinated and nonvaccinated syngeneic c57b1-6 mice to infection with mycobacterium tuberculosis. | data are presented which show that the syngeneic c57b1/6 mouse strain is far more susceptible to infection with mycobacterium tuberculosis strain h37rv than are the other allogeneic mouse strains used in this laboratory, particularly the strong a and the cf-1 strains. not only are the c57b1/6 mice more susceptible to tuberculous infection, but also they respond to infection more uniformly than do the allogeneic strains. c57b1/6 mice develop immunity to challenge with small infecting doses of the ... | 1972 | 4629204 |
| effect of isoniazid on the carbohydrate metabolism of isoniazid-susceptible and isoniazid-resistant mycobacterium tuberculosis h37rv. | | 1973 | 4630310 |
| effective nonliving vaccine against experimental tuberculosis in mice. | ribi, edgar (rocky mountain laboratory, hamilton, mont.), carl larson, william wicht, robert list, and granville goode. effective nonliving vaccine against experimental tuberculosis in mice. j. bacteriol. 91:975-983. 1966.-antituberculosis vaccines were prepared in one of three manners: lyophilized bcg suspended in light mineral oil was disrupted in a sorvall pressure cell and the "oil disruption product" was collected by centrifugation; bcg was disrupted in water, lyophilized, and worked into a ... | 1966 | 4956339 |