| a new cerebroside from fusicoccum amygdali del. | a homogeneous cerebroside was isolated from a strain of fusicoccum amygdali del., a fungus pathogenic to almond and peach. chemical degradations, together with extensive application of nuclear magnetic resonance and mass spectrometry techniques, led to elucidation of its structure. this corresponds to n-2'-hydroxy-3'-trans-octadecenoyl-1-o-beta-d-glucosyl-9-methyl-cis-4,x-8-sphingadienine. | 1979 | 454639 |
| characterization of by-products of fusicoccin in culture filtrates of fusicoccum amygdali del. | | 1970 | 4191500 |
| characterization of two cell-wall polysaccharides from fusicoccum amygdali. | 1. the nature of two polysaccharides (s(0) (20) values 6s and 2s respectively in 1m-sodium hydroxide), comprising a fragment (fraction bb, [alpha](d) +236 degrees in 1m-sodium hydroxide), previously isolated from cell walls of fusicoccum amygdali, has been investigated. 2. both the major (2s) and minor (6s) components were affected by incubation with alpha-amylase. the 6s polysaccharide was also attacked by exo-beta-(1-->3)-glucanase, which is evidence that it contained both alpha-(1-->4)- and b ... | 1971 | 4335690 |
| 12-o-acetylfusicoccin and 12-o-acetylisofusicoccin, two new minor metabolites of fusicoccum amygdali del. | | 1974 | 4435100 |
| the composition of the cell wall of fusicoccum amygdali. | 1. the cell wall of fusicoccum amygdali consisted of polysaccharides (85%), protein (4-6%), lipid (5%) and phosphorus (0.1%). 2. the main carbohydrate constituent was d-glucose; smaller amounts of d-glucosamine, d-galactose, d-mannose, l-rhamnose, xylose and arabinose were also identified, and 16 common amino acids were detected. 3. chitin, which accounted for most of the cell-wall glucosamine, was isolated in an undegraded form by an enzymic method. chitosan was not detected, but traces of gluc ... | 1971 | 5144751 |
| the wilting toxins of fusicoccum amygdali del. | | 1969 | 5810064 |
| role of the 14-3-3 proteins in the regulation of h+-atpase activity in the plasma membrane of suspension-cultured sugar beet cells under cold stress. | all higher plants possess highly specific binding sites for fusicoccin, a metabolite of the fungus fusicoccum amygdali del. these sites are harboured in the plasma membranes and formed by a 14-3-3 protein dimer associated with the c-terminal autoinhibitory domain of h+-atpase. we considered the fusicoccin binding to plasma membranes to be an indicator of complexation between the 14-3-3 dimer and h+-atpase, we assessed the effect of cold stress on the interaction of these proteins in suspension-c ... | 1999 | 10452522 |
| (+)-menthol and its hydroxy derivatives, novel fungal monoterpenols from the fusicoccin-producing fungi, phomopsis amygdali f6a and niigata 2. | in our search for new fusicoccins of unique diterpene glucosides from phomopsis amygdali, we found that a fragrant substance was formed in the early stage of fusicoccin fermentation. this fragrant constituent was isolated and identified as (+)-menthol, which is a novel fungal metabolite as the enantiomer of well-known peppermint (-)-menthol. (+)-7-hydroxymenthol and new (+)-(6s)-hydroxymenthol were also isolated and identified as fungal metabolites. in addition, p-menthanetriol, which has been r ... | 2003 | 12723593 |
| fusicoccin signaling reveals 14-3-3 protein function as a novel step in left-right patterning during amphibian embryogenesis. | to gain insight into the molecular mechanisms underlying the control of morphogenetic signals by h+ flux during embryogenesis, we tested fusicoccin-a (fc), a compound produced by the fungus fusicoccum amygdali del. in plant cells, fc complexes with 14-3-3 proteins to activate h+ pumping across the plasma membrane. it has long been thought that fc acts on higher plants only; here, we show that exposing frog embryos to fc during early development specifically results in randomization of the asymme ... | 2003 | 12930777 |
| the growth of fusicoccum amygdali delacroix in vitro as influenced by ph, phenolic compounds, and unpurified extracts from peach twigs. | | 1964 | 14211342 |
| novel fusicoccins r and s, and the fusicoccin s aglycon (phomopsiol) from phomopsis amygdali niigata 2-a, and their seed germination-stimulating activity in the presence of abscisic acid. | our search for new 3-hydroxyfusicoccins structurally related to cotylenin a from a culture of phomopsis amygdali niigata 2-a resulted in the isolation of novel 3-hydroxy fusicoccins, called fusicoccins r and s, and the fusicoccin s aglycon, called phomopsiol, together with known 3alpha-hydroxyfusicoccin j. the structure of phomopsiol was identified as that of o-demethyl-3-epicotylenol based on spectroscopic evidence. the structures of fusicoccins r and s were also determined to be those of 3'-de ... | 2004 | 15170119 |
| identification of (+)-phyllocladene, (--)-sandaracopimaradiene, and (+)-kaurene as new fungal metabolites from fusicoccin-producing phomopsis amygdali f6. | a chemical analysis of the diterpene hydrocarbons produced by fusicoccin-producing fungus phomopsis amygdali f6 identified five phyllocladene-related tri- and tetracyclic diterpene hydrocarbons. the presence of (+)-phyllocladene, (--)-sandaracopimaradiene, (+)-isopimara-8,15-diene, and (+)-pimara-8(14),15-diene in the fungus was demonstrated by gc-ms, 1h-nmr, and [alpha]d measurements. (+)-kaurene was also identified by gc-ms and chiral capillary gc. the possible biosynthetic relationship of the ... | 2004 | 15277764 |
| fusicocca-3(16),10(14)-diene, and beta- and delta-araneosenes, new fusicoccin biosynthesis-related diterpene hydrocarbons from phomopsis amygdali. | further isolation and examination of fusicoccane hydrocarbons biosynthetically related to fusicoccin from phomopsis amygdali allowed us to identify new fungal diterpene hydrocarbons of fusicoccadiene and araneosene. these were assigned as (+)-fusicocca-3(16),10(14)-diene, and (+)-beta- and (+)-delta-araneosenes. these findings led to the experimental clarification of the structures of the biosynthetic hydrocarbon intermediates presumed earlier. | 2004 | 15277774 |
| fusicoccins are biosynthesized by an unusual chimera diterpene synthase in fungi. | fusicoccins are a class of diterpene glucosides produced by the plant-pathogenic fungus phomopsis amygdali. as modulators of 14-3-3 proteins, fusicoccins function as potent activators of plasma membrane h(+)-atpase in plants and also exhibit unique biological activity in animal cells. despite their well studied biological activities, no genes encoding fusicoccin biosynthetic enzymes have been identified. cyclic diterpenes are commonly synthesized via cyclization of a c(20) precursor, geranylgera ... | 2007 | 17360612 |
| identification of diterpene biosynthetic gene clusters and functional analysis of labdane-related diterpene cyclases in phomopsis amygdali. | two diterpene biosynthesis gene clusters in the fusicoccin-producing fungus, phomopsis amygdali, were identified by genome walking from paggs1 and paggs4 which encode the geranylgeranyl diphosphate (ggdp) synthases. the diterpene cyclase-like genes, padc1 and padc2, were respectively located proximal to paggs1 and paggs4. the amino acid sequences of these two enzymes were similar to those of fungal labdane-related diterpene cyclases. recombinant padc1 converted ggdp mainly into phyllocladan-16 a ... | 2008 | 18391465 |
| recent advances regarding diterpene cyclase genes in higher plants and fungi. | cyclic diterpenoids are commonly biosynthesized from geranylgeranyl diphosphate (ggdp) through the formation of carbon skeletons by specific cyclases and subsequent chemical modifications, such as oxidation, reduction, methylation, and glucosidation. a variety of diterpenoids are produced in higher plants and fungi. rice produces four classes of diterpene phytoalexins, phytocassanes a to e, oryzalexins a to f, oryzalexin s, and momilactones a and b. the six diterpene cyclase genes involved in th ... | 2008 | 18460786 |
| seasonal availability of inoculum for constriction canker of peach in new jersey. | abstract the seasonal sporulation ability of phomopsis amygdali was evaluated over a 2-year period by sampling twig cankers from commercial 'encore' and 'jerseyglo' peach orchards. canker size, pycnidia per canker, percent pycnidia forming cirri, and spore production were evaluated once each month from january 1997 through december 1998. average canker size and number of pycnidia per canker, which followed a sinusoidal pattern of change, were at lowest values in spring and at maximum values in f ... | 2001 | 18943448 |
| quantitative models for describing temperature and moisture effects on sporulation of phomopsis amygdali on peach. | abstract twigs with constriction cankers were pruned from a prunus persica 'jerseyglo' orchard and placed in incubators under high humidity (>95%) at constant temperatures of -6, 1, 10, 17, 24, 31, 38, and 45 degrees c. cankers were removed for observation after 2, 6, 24, 48, 72, and 96 h incubation. sporulation was expressed as the percentage of pycnidia producing cirri and the number of conidia per pycnidium or canker. the experiment was first performed during 1998 to 1999 and then repeated in ... | 2003 | 18944102 |
| biosynthetic gene-based secondary metabolite screening: a new diterpene, methyl phomopsenonate, from the fungus phomopsis amygdali. | the presence of the geranylgeranyl diphosphate synthase (ggs) gene is a common feature of gene clusters for diterpene biosynthesis. we demonstrated identification of a diterpene gene cluster using homology-based pcr of ggs genes and the subsequent genome walking in the fungus phomopsis amygdali n2. structure determination of a novel diterpene hydrocarbon phomopsene provided by enzymatic synthesis with the recombinant terpene synthase paps and screening of fungal broth extracts with reference to ... | 2009 | 19161275 |
| toxicity of leaf and stem extracts to tylenchorhynchus dubius. | plant extracts, made by grinding 2 g of fresh tissue in 5 ml of water, were toxic to tylenchorhynchus dubius and hoplolaimus spp. such extracts from leaves and stems of bean (phaseolus vulgaris l.) and leaves of tobacco (nicotiana tabacum l.) were most toxic; those from leaves of corn (zea mays l.), tomato (lycopersicon esculentum mill.) and rhododendron (rhododendron catawbiense l.) were less toxic; and extracts of bean roots were nontoxic. nematode movement slowed markedly within 1 hr in tobac ... | 1973 | 19319327 |
| a new selective medium for the recovery and enumeration of monilinia fructicola, m. fructigena, and m. laxa from stone fruits. | isolation of monilinia spp. from stone and pome fruit surfaces is difficult due to the presence of several fast-growing fungal species such as rhizopus, alternaria, and penicillium spp. therefore, a new selective medium (acidified potato dextrose agar [ph 3.6] amended with fosetyl-aluminum [fosetyl-al] at 500 microg/ml) (apda-f500) was developed for the recovery of monilinia propagules. the antifungal agents fosetyl-al, dichloran, ammonium molybdate, and 2-deoxy-d-glucose (2-dd-glucose) were tes ... | 2009 | 19740034 |