| cloning of genes required for hypersensitivity and pathogenicity in pseudomonas syringae pv. aptata. | a genomic library of pseudomonas syringae pv. aptata strain ncppb 2664, which causes bacterial blight of sugar beet, lettuce and other plants, was constructed in the cosmid vector pcpp31. the 13.4 kb ecori fragment of the cosmid phir11, containing the hrp (hypersensitive response and pathogenicity) gene cluster of the closely related bacterium pseudomonas syringae pv. syringae strain 61, was used as a probe to identify a homologous hrp gene cluster in p. syringae pv. aptata. thirty of 2500 cosmi ... | 1995 | 7771767 |
| characterization of fluorescent and nonfluorescent peptide siderophores produced by pseudomonas syringae strains and their potential use in strain identification. | nonfluorescent highly virulent strains of pseudomonas syringae pv. aptata isolated in different european countries and in uruguay produce a nonfluorescent peptide siderophore, the production of which is iron repressed and specific to these strains. the amino acid composition of this siderophore is identical to that of the dominant fluorescent peptide siderophore produced by fluorescent p. syringae strains, and the molecular masses of the respective fe(iii) chelates are 1,177 and 1,175 atomic mas ... | 2001 | 11282626 |
| identification and expression of the pseudomonas syringae pv. aptata hrpz(psa) gene which encodes an harpin elicitor. | a sequence homologous to an internal fragment 0.75 kb bstxi of the pseudomonas syringae pv. syringae hrpz gene was identified in pseudomonas syringae pv. aptata ncppb 2664, the causal agent of bacterial blight in sugar beet, lettuce and other plants. and in e. coli dh10b (pccp1069) containing the p. syringae pv. aptata hrp gene cluster. pcr with oligonucleotides, based on the hrpz(pss) gene and used as primers with the total genomic dna of p. syringae pv. aptata, amplified a 1 kb fragment that h ... | 2001 | 11392485 |