| community-acquired bacteremia caused by serratia plymuthica. case report and review of the literature. | we present a case of a 50-year-old woman who was admitted to the hospital as a result of a process affecting the lower respiratory tract, associated with a community-acquired bacteremia due to serratia plymuthica. the patient was treated with erythromycin and gentamicin and was discharged to home 7 days after admission with complete clinical cure. in view of the lack of reports concerning human infections by s. plymuthica, we present our case and review the literature concerning the microbiologi ... | 1992 | 1643822 |
| isolation of cb-25-i, an antifungal antibiotic, from serratia plymuthica. | a new antifungal antibiotic, cb-25-i, was isolated from the culture broth of a strain of serratia plymuthica. the antibiotic, a water-soluble dipeptide, is structurally related to sch 37137 and a 19009, both produced by strains of actinomycetales. the antibiotic exhibits inhibitory activity against candida albicans in ynb medium (a synthetic medium), but the activity is significantly reduced in sabouroud dextrose medium. | 1989 | 2500411 |
| isolation of serratia plymuthica from a human burn site. | the saprophytic bacterium serratia plymuthica was recovered from a facial wound (burn) site of a pediatric patient. the clinical significance of the organism was undetermined due to its apparent eradication from this location by therapy with topical 1% silver sulfadiazine. seeding of the burn with s. plymuthica may have occurred from contaminated moisture sometimes found on and around steam radiators. | 1985 | 3988909 |
| structural and serological characterisation of an o-specific polysaccharide from serratia plymuthica. | the surface polysaccharides of a strain of serratia plymuthica were characterised and shown to consist of a linear, acidic galactoglucomannan as well as a major and a minor neutral galactan. immunoblotting results demonstrated cross-reactions between this strain and others with similar galactans (s. marcescens o16 and o20, klebsiella o1, and pasteurella haemolytica t4 and t10). | 1993 | 7691682 |
| nosocomial septicemia caused by serratia plymuthica. | we report a case of nosocomial septicemia in a 79-year-old patient caused by serratia plymuthica with no evident focus of infection. the patient was treated with gentamicin (40 mg every 8 h) during 10 days; clinical resolution of the infection was obtained after the 10-day treatment period. | 1994 | 8150981 |
| incidence of histamine-forming bacteria and histamine content in scombroid fish species from retail markets in the barcelona area. | incidence and diversity of histidine decarboxylating bacteria were determined in samples of tunafish, bonito and mackerel purchased at different retail markets. histamine-forming bacteria occurred in a low proportion and always accounted for less than 0.1% of the total bacterial load in the fish samples studied. similarly, histamine content in fish samples also was low ( < 25 ppm) and all of them met current histamine standards established by the european union. histamine was found in 83.3% of t ... | 1996 | 8652349 |
| capd peritonitis due to serratia plymuthica. | | 2000 | 10898057 |
| evaluation of the id 32e for the identification of gram-negative glucose-fermenting and glucose-non-fermenting bacilli. | objective: to evaluate the id 32e bacterial identification system for accuracy in the identification of members of the family enterobacteriaceae, pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii/iwoffii. methods: stock cultures of 497 enterobacteriaceae and 27 commonly encountered non-enteric gram-negative rods were tested in the id 32e system. for each isolate, the resulting 11-digit profile number was converted to an identification using the apilab plus softwar ... | 1999 | 11856267 |
| natural antimicrobial susceptibilities of strains of 'unusual' serratia species: s. ficaria, s. fonticola, s. odorifera, s. plymuthica and s. rubidaea. | the natural susceptibility to 71 antibiotics of 104 serratia strains of serratia ficaria (n = 15), serratia fonticola (n = 18), serratia odorifera (n = 16), serratia plymuthica (n = 32) and serratia rubidaea (n = 23) was examined. mics were determined using a microdilution procedure in isosensitest broth for all the strains and in cation-adjusted mueller-hinton broth for some strains. with few exceptions, all species tested were uniformly naturally resistant to penicillin g, oxacillin, cefazolin ... | 2003 | 12654765 |
| a molecular phylogeny of enteric bacteria and implications for a bacterial species concept. | a molecular phylogeny for seven taxa of enteric bacteria (citrobacter freundii, enterobacter cloacae, escherichia coli, hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, and serratia plymuthica) was made from multiple isolates per taxa taken from a collection of environmental enteric bacteria. sequences from five housekeeping genes (gapa, groel, gyra, ompa, and pgi) and the 16s rrna gene were used to infer individual gene trees and were concatenated to infer a composite molecular phylogen ... | 2003 | 14640415 |
| necrotic cellulitis by serratia plymuthica. | this case report describes an uncommon cellulitis caused by serratia plymuthica in a patient treated with steroids. the evolution was favorable after surgical exploration with debridement and antibiotic treatment. this is the first case of necrotic cellulitis caused by s. plymuthica described in the literature. | 2003 | 14962705 |
| the global regulator genes from biocontrol strain serratia plymuthica ic1270: cloning, sequencing, and functional studies. | the biocontrol activity of various fluorescent pseudomonads towards plant-pathogenic fungi is dependent upon the gaca/gacs-type two-component system of global regulators and the rpos transcription sigma factor. in particular, these components are required for the production of antifungal antibiotics and exoenzymes. to investigate the effects of these global regulators on the expression of biocontrol factors by plant-associated bacteria other than pseudomonas spp., gaca/gacs and rpos homologues w ... | 2004 | 15262936 |
| characterization of serratia isolates from soil, ecological implications and transfer of serratia proteamaculans subsp. quinovora grimont et al. 1983 to serratia quinivorans corrig., sp. nov. | eleven strains of serratia were isolated from different soils and the guts of invertebrates and characterized by their sensitivity to eight indigenous bacteriophages. they were also classified according to bacteriocin production and sensitivity, biolog plate and api 20e strip profiles and 16s rrna sequence information. one strain was thus identified as serratia plymuthica, another as serratia fonticola. the remaining strains were shown to be closely related to serratia proteamaculans subsp. quin ... | 2002 | 12508898 |
| effect of plant species on the kinetics of conjugal transfer in the rhizosphere and relation to bacterial metabolic activity. | conjugal transfer of a derivative of the rp4 plasmid between pseudomonas fluorescens as12 and serratia plymuthica rf7 was compared in the rhizosphere of pea, wheat, and barley and related to the metabolic activity of the bacteria. to obtain a reliable measure of transfer, which allowed comparison of results between experiments, mathematical mass-action models were used to determine plasmid intrinsic kinetic coefficients. the data showed that not only were the rhizospheres highly conducive of tra ... | 2001 | 12024270 |
| isolation and identification of synthetic pyrethroid-degrading bacteria. | to isolate, select, identify and assess the potential for the biodegradation of synthetic pyrethroids (sps) in sheep dips. | 2002 | 11872130 |
| quorum sensing dependent production of antimicrobial component influences establishment of e. coli in dual species biofilms with serratia plymuthica. | | 2005 | 16366307 |
| evaluation of potential biocontrol rhizobacteria from different host plants of verticillium dahliae kleb. | a screening approach was developed to assess the potential of rhizobacterial strains to control verticillium wilt caused by verticillium dahliae kleb. | 2001 | 11851803 |
| isolation and functional analysis of luxs in serratia plymuthica rvh1. | autoinducer-2 (ai-2) is a signalling molecule proposed to act as a 'universal' signal for interspecies communication, regulating niche-specific genes with diverse functions in various bacteria. we have previously reported the production of ai-2 in strains from different serratia species and recently, the luxs gene of two serratia strains was isolated and shown to affect distinct phenotypes. in the current work, we have identified the luxs gene of serratia plymuthica rvh1, and studied its effect ... | 2006 | 16923076 |
| role of quorum sensing and antimicrobial component production by serratia plymuthica in formation of biofilms, including mixed biofilms with escherichia coli. | we have previously characterized the n-acyl-l-homoserine lactone-based quorum-sensing system of the biofilm isolate serratia plymuthica rvh1. here we investigated the role of quorum sensing and of quorum-sensing-dependent production of an antimicrobial compound (ac) on biofilm formation by rvh1 and on the cocultivation of rvh1 and escherichia coli in planktonic cultures or in biofilms. biofilm formation of s. plymuthica was not affected by the knockout of spli or splr, the s. plymuthica homologs ... | 2006 | 16997989 |
| purification and properties of two chitinolytic enzymes of serratia plymuthica hro-c48. | the chitinolytic rhizobacterium serratia plymuthica hro-c48 was previously selected as a biocontrol agent of phytopathogenic fungi. one endochitinase (e.c. 3.2.1.14), chit60, and one n-acetyl-beta-1,4- d-hexosaminidase (e.c. 3.2.1.52), chit100, were purified and characterized. the endochitinase chit60, with an apparent molecular mass of 60.5 kda, had a n-terminal amino acid sequence highly similar to that of chitinases a from serratia liquefaciens and serratia marcescens. the enzyme activity had ... | 2001 | 11734885 |
| role of quorum sensing in resistance of serratia plymuthica against acanthamoeba castellanii. | | 2006 | 17191532 |
| characterization of a luxi/luxr-type quorum sensing system and n-acyl-homoserine lactone-dependent regulation of exo-enzyme and antibacterial component production in serratia plymuthica rvh1. | quorum sensing by means of n-acyl-l-homoserine lactones (ahls) is widespread in gram-negative bacteria, where diverse ahls influence a wide variety of functions, even in a single genus such as serratia. here we report the identification and characterization of the quorum sensing system of serratia plymuthica strain rvh1. this strain isolated from a raw vegetable processing line produces at least three ahls which were identified as n-butanoyl- (c4-hsl), n-hexanoyl- (c6-hsl) and n-(3-oxo-hexanoyl) ... | 2007 | 17258895 |
| diversity of antifungal and plant-associated serratia plymuthica strains. | a total of 21 plant-associated serratia plymuthica strains were characterized phenotypically by their nutritional patterns, susceptibility to antibiotics, antifungal and haemolytic properties, and genotypically by denaturing gradient gel electrophoresis (dgge) of pcr-amplified 16s rdna, pcr fingerprints using box primers (box-pcr) and pulsed-field gel electrophoresis (pfge) after digestion with spei. all of the investigated strains demonstrated antifungal activity in vitro against fungal pathoge ... | 2000 | 10849170 |
| identification of environmental serratia plymuthica strains with the new combo panels type 1s. | automated systems are required when numerous samples need to be processed, offering both high through put and test of a multiple simultaneously. this study was performed to compare the microscan walkaway automated identification system in conjunction with the new microscan combo neg panels type 1s with conventional biochemical methods for identifying ten environmental serratia plymuthica strains. high correlation between both methods were observed for all the 21 tests evaluated, and the microsca ... | 2000 | 10733744 |
| the spoilage flora of vacuum-packaged, sodium nitrite or potassium nitrate treated, cold-smoked rainbow trout stored at 4 degrees c or 8 degrees c. | the spoilage flora of vacuum-packaged, salted, cold-smoked rainbow trout fillets, with or without the addition of nitrate or nitrite, stored at 4 degrees c and 8 degrees c, was studied. of 620 isolates, lactic acid bacteria were the major fraction (76%), predominating in all samples of spoiled product. however, the phenotypical tests used were insufficient to identify the lactic acid bacteria to the species level. gram-positive, catalase-positive cocci, gram-negative, oxidase-negative rods and g ... | 1998 | 9924944 |
| proposition for the biochemical mechanism occurring in the sucrose isomerase active site. | sucrose conversion by sucrose isomerase from protaminobacter rubrum, serratia plymuthica and erwinia rhapontici was investigated in the presence of different monosaccharides in the reaction mixture. these conditions led to inhibitory effects and to glucosyl transfer of the glucose moiety of sucrose to the exogen monosaccharide. comparison of the structure of the different inhibitors and acceptors has allowed us to suppose the binding of the sucrose molecule into the active site, and thereafter, ... | 1998 | 9891968 |
| mass balance studies with 14c-labeled 2,4,6-trinitrotoluene (tnt) mediated by an anaerobic desulfovibrio species and an aerobic serratia species. | investigations were carried out to evaluate the level of incorporation of radiolabeled 2,4,6-trinitrotoluene (tnt) and metabolites into the bacterial biomass of two different bacterial species after cometabolically mediated tnt transformation. biotransformation experiments with 14c-tnt indicated that tnt was not mineralized; however, carbon derived from tnt became associated with the cells. it was found that more than 42% of the initially applied radiolabel was associated with the cell biomass a ... | 1998 | 9806975 |
| [septic shock by serratia plymuthica]. | | 1997 | 9101738 |
| microbial contamination of contact lens cases among medical students. | we performed a systematic examination of the rate and level of contamination of contact lens cases in a population of asymptomatic contact lens wearers using commercially prepared chemical disinfection solutions. | 1996 | 8835064 |
| report of six cases of human infection by serratia plymuthica. | serratia plymuthica is an uncommon cause of human infection. only one case of chronic osteomyelitis and two cases of sepsis secondary to central venous catheter infection have been documented. we report the isolation of s. plymuthica from six patients. the organism was recovered from blood cultures in three cases in which the patients had lymphoblastic leukemia, lymphoma, or stroke. two isolates were recovered from exudates (following knee and abdominal surgery). in the last case, the organism w ... | 1995 | 7714177 |
| new fimbrial hemagglutinin in serratia species. | strains of serratia marcescens, serratia liquefaciens, serratia marinorubra, and serratia plymuthica produced one or more of the following hemagglutinins (has): mannose-sensitive ha and mannose-resistant k-ha (mr/k-ha) and p-ha (mr/p-ha) (j. p. duguid and d. c. old, in e. h. beachey (ed.), bacterial adherence, vol. 6., p. 185-217, 1980). most strains (82%) were multiply hemagglutinating. the properties of the three has are described. each ha was associated with a distinct type of fimbria: mannos ... | 1982 | 6128303 |
| [studies on pigment formation and pigment dissociation in serratia plymuthica (lehm. et neum.) bergey et al]. | | 1967 | 5632363 |
| serratia plymuthica sepsis associated with infection of central venous catheter. | serratia plymuthica was isolated from the blood and from a central venous catheter tip in a 54-year-old man with alcoholic cirrhosis and clinical signs of sepsis. this was the seventh reported isolate of s. plymuthica from a clinical specimen and the first in which the organism was clearly a significant pathogen. | 1987 | 3624450 |
| serratia plymuthica osteomyelitis following a motorcycle accident. | serratia plymuthica was isolated from the sinus tract and from the infected bone in a case of chronic osteomyelitis following an accident of a motorcyclist. this is the second case in which s. plymuthica was a significant pathogen. previously, a case of s. plymuthica sepsis associated with infection of a central venous catheter was described. | 1988 | 3410953 |
| differential effectiveness of serratia plymuthica ic1270-induced systemic resistance against hemibiotrophic and necrotrophic leaf pathogens in rice. | induced resistance is a state of enhanced defensive capacity developed by a plant reacting to specific biotic or chemical stimuli. over the years, several forms of induced resistance have been characterized, including systemic acquired resistance, which is induced upon localized infection by an avirulent necrotizing pathogen, and induced systemic resistance (isr), which is elicited by selected strains of nonpathogenic rhizobacteria. however, contrary to the relative wealth of information on indu ... | 2009 | 19161601 |
| endophytic and ectophytic potato-associated bacterial communities differ in structure and antagonistic function against plant pathogenic fungi. | differences between endophytic and ectophytic bacterial communities with stress on antagonistic bacteria, were studied by comparing the composition of communities isolated from the rhizosphere, phyllosphere, endorhiza and endosphere of field-grown potato plants using a multiphasic approach. terminal restriction fragment length polymorphism analysis of 16s rdna of the bacterial communities revealed discrete microenvironment-specific patterns. to measure the antagonistic potential of potato-associ ... | 2005 | 16329870 |
| activity of serratia plymuthica ic1270 gene chia promoter region in escherichia coli mutants deficient in global regulators of transcription. | to study the regulation of expression of the serratia plymuthica gene chia encoding a 58-kda endochitinase, its 586-bp-long upstream regulatory region was cloned, sequenced and fused to a promoterless lac operon in phage lambdars45 to obtain a single-copy transcriptional fusion (p f1chia )-lac in lysogens of escherichia coli wild-type strains or their mutants deficient in various global regulators of transcription. the level of p f1chia -lac expression increased about 20- and 90-fold, respective ... | 2005 | 16304705 |
| effectiveness of 3 antagonistic bacterial isolates to control rhizoctonia solani kühn on lettuce and potato. | rhizoctonia solani causes yield losses in numerous economically important european crops. to develop a biocontrol strategy, 3 potato-associated ecto- and endophytically living bacterial strains pseudomonas fluorescens b1, pseudomonas fluorescens b2, and serratia plymuthica b4 were evaluated against r. solani in potato and in lettuce. the disease-suppression effect of the 3 biocontrol agents (bcas) was tested in a growth chamber and in the field. in growth chamber experiments, all 3 bcas complete ... | 2005 | 15980897 |
| genotypic and phenotypic characterization of a biofilm-forming serratia plymuthica isolate from a raw vegetable processing line. | recently, we isolated from a raw vegetable processing line a serratia strain with strong biofilm-forming capacity and which produced n-acyl-l-homoserine lactones (ahls). within the enterobacteriaceae, strains of the genus serratia are a frequent cause of human nosocomial infections; in addition, biofilm formation is often associated with persistent infections. in the current report, we describe the detailed characterization of the isolate using a variety of genotypic and phenotypic criteria. alt ... | 2005 | 15899415 |
| strategy to select and assess antagonistic bacteria for biological control of rhizoctonia solani kühn. | a screening strategy was developed to assess the potential of plant-associated bacteria to control diseases caused by rhizoctonia solani kühn. about 434 already characterized antagonistic bacterial strains isolated from diverse plant species and microenvironments were evaluated for biocontrol and plant growth promotion by a hierarchical combination of assays. analyzing in vitro antagonism towards different rhizoctonia isolates resulted in a selection of 20 potential biocontrol agents. the strain ... | 2004 | 15644895 |
| studies on the occurrence of gram-negative bacteria in ticks: ixodes ricinus as a potential vector of pasteurella. | a total of 372 ixodes ricinus ticks (101 females, 122 males, and 149 nymphs) collected by flagging in 6 mixed woodlands of eastern poland were examined by culture for the presence of internal gram-negative bacteria other than borrelia burgdorferi. adult ticks were examined in pools of 2 specimens each and nymphs were examined in pools of 3-5 specimens each. ticks were disinfected in 70 % ethanol and homogenized in 0.85% nacl. the diluted homogenate was inoculated onto 3 kinds of agar media: buff ... | 2004 | 15627343 |
| production of palatinose using serratia plymuthica cells immobilized in chitosan. | in recent decades, the production of palatinose has aroused great interest since this structural isomer of sucrose has interesting potential. we describe a simple and effective method of immobilizing serratia plymuthica cells in chitosan. the sucrose isomerase activity of immobilized preparations was enhanced many times by activation with fresh nutrient medium and subsequent drying. the preparations obtained were physically very stable with high enzyme activity and excellent operational stabilit ... | 2003 | 12955543 |
| isolation of plasmid-harboring serratia plymuthica from facultative gut microflora of the tobacco hornworm, manduca sexta. | aseptic isolation of the facultative gut microflora of the tobacco hornworm, manduca sexta, yielded four microorganisms. two were gram-positive bacillus spp., one was serratia plymuthica, and another was the yeast candida guilliermondii. the three bacterial species were screened for extrachromosomal dna, and s. plymuthica was found to have a 6.4-kilobase plasmid, which was designated pcp-1. | 1988 | 16347696 |
| successive microbial populations in calimyrna figs. | smyrna-type (calimyrna) figs have essentially sterile internal tissue until visited by the pollinating fig wasp, blastophaga psenes, which introduces a specific microflora consisting of candida guilliermondii var. carpophila and serratia plymuthica. this flora persists and develops in numbers throughout the ripening period until maturity of the fruit. these organisms do not cause spoilage. the presence of c. guilliermondii var. carpophila appears to increase the attractiveness of the fruit to dr ... | 1962 | 16349620 |
| n-acyl-l-homoserine lactone quorum sensing controls butanediol fermentation in serratia plymuthica rvh1 and serratia marcescens mg1. | butanediol fermentation in two serratia species is shown to be affected by n-acyl-l-homoserine lactone-dependent quorum sensing. knockout of quorum-sensing signal production caused a shift towards enhanced acid production, resulting in early growth arrest, which was reversible by the addition of synthetic signal molecules. | 2006 | 16740963 |
| preferential feeding by the ciliates chilodonella and tetrahymena and their effects on bacterial biofilm structure and composition. | protozoa are important components of microbial food webs, but protozoan feeding preferences and their effects in the context of bacterial biofilms are not well understood. the feeding interactions of two contrasting ciliates, the free-swimming filter feeder tetrahymena sp. and the surface-associated predator chilodonella sp, were investigated using biofilm-forming bacteria genetically modified to express fluorescent proteins. according to microscopy, both ciliates readily consumed cells from bot ... | 2011 | 21602372 |
| volatiles of bacterial antagonists inhibit mycelial growth of the plant pathogen rhizoctonia solani. | bacterial antagonists are bacteria that negatively affect the growth of other organisms. many antagonists inhibit the growth of fungi by various mechanisms, e.g., secretion of lytic enzymes, siderophores and antibiotics. such inhibition of fungal growth may indirectly support plant growth. here, we demonstrate that small organic volatile compounds (vocs) emitted from bacterial antagonists negatively influence the mycelial growth of the soil-borne phytopathogenic fungus rhizoctonia solani kühn. s ... | 2007 | 17180381 |
| quorum-sensing signaling is required for production of the antibiotic pyrrolnitrin in a rhizospheric biocontrol strain of serratia plymuthica. | one mechanism that bacteria have adopted to regulate the production of antimicrobial compounds is population-density-dependent luxri-type quorum sensing (qs), exploiting the production of n-acyl homoserine lactone (ahl) autoinducer signals. in biocontrol bacteria, most known cases involve the ahl control of phenazine antibiotics production by rhizospheric pseudomonads. this work is the first to demonstrate that phenazines are not the only group of biocontrol-related antibiotics whose production ... | 2007 | 17355597 |
| pythium perplexum isolated from soil in france: morphology, molecular characterisation and biological control. | pythium perplexum (f-926) was isolated from a soil sample taken in the burgundy region in france. in 1907, it was mistakenly described by bulter as p. vexans. despite morphological resemblance, the comparison between the internal transcribed spacer 1 regions of the ribosomal dna of the two fungi leaves no doubt of their different identities. p. perplexum was found to be highly pathogenic to cucumber seedlings. damping-off disease of cucumber caused by p. perplexum can effectively be controlled b ... | 2001 | 11572459 |
| Role of the RNA-binding protein Hfq in Serratia plymuthica. | The RNA-binding protein Hfq has been well studied as a global post-transcriptional regulator which controls diverse cellular processes in bacteria. However, the function in the genus of Serratia has remained unexplored. Here we show that beyond mutation in Hfq resulting in their growth defects, Hfq has global effects on a variety of biocontrol-related phenotypes in the endophytic strain G3 of Serratia plymuthica, including antifungal activity, production of exoenzymes, as well as motility and bi ... | 2012 | 22201952 |
| quorum-sensing-dependent switch to butanediol fermentation prevents lethal medium acidification in aeromonas hydrophila ah-1n. | aeromonas hydrophila ah-1n shows biphasic growth in glucose containing broth at 30 degrees c. the first phase is characterized by strong acidification, while the second phase is accompanied by an increase in medium ph. disruption of ahl production by knockout of ahyi did not affect the first growth phase, but resulted in further acidification which completely blocked the second growth phase and even caused cell death. the block could be relieved by addition of 5 microm (or less) synthetic n-buta ... | 2007 | 17408926 |
| [cloning, sequencing and fuctional study of gaca gene from xanthomonas oryzae pv. oryzicola]. | a gaca homologue, designated gaca(xooc), was cloned from xanthomonas oryzae pv. oryzicola (xooc), a bacterium that causes leaf streak of rice, with degenerated primers by polymerase amplification reaction (pcr). ncbi blast search indicated that gaca(xooc) had a similar structure to that of other gaca proteins, and had a cheb (chemotaxis response regulator containing a chey-like receiver domain)domain. sequence comparison showed that the gaca(xooc) was conserved in the xanthomonas genus. homology ... | 2007 | 17552221 |
| complete genome sequence of the plant-associated serratia plymuthica strain as13. | serratia plymuthica as13 is a plant-associated gammaproteobacteria, isolated from rapeseed roots. it is of special interest because of its ability to inhibit fungal pathogens of rapeseed and to promote plant growth. the complete genome of s. plymuthica as13 consists of a 5,442,549 bp circular chromosome. the chromosome contains 4,951 protein-coding genes, 87 trna genes and 7 rrna operons. this genome was sequenced as part of the project entitled "genomics of four rapeseed plant growth promoting ... | 2012 | 23450001 |
| the growth of fungi and arabidopsis thaliana is influenced by bacterial volatiles. | dual culture systems, which only allowed volatiles to cross the boundary of a bipartite petri dish, were used to investigate the effects of bacterial volatiles on the growth of 14 fungi and a. thaliana. the majority of tested combinations exhibited dramatic growth retardations of fungi and a. thaliana, indicating that volatiles can act as antibiotics. it therefore can be concluded that bacterial volatiles influence the growth conditions of organisms in a community and in a habitat. | 2008 | 19704493 |
| effects of quorum sensing on the spoilage of food by serratia plymuthica. | | 2007 | 18018912 |
| effect of bacterial antagonists on lettuce: active biocontrol of rhizoctonia solani and negligible, short-term effects on nontarget microorganisms. | the aim of this study was to assess the biocontrol efficacy against rhizoctonia solani of three bacterial antagonists introduced into naturally rhizoctonia-infested lettuce fields and to analyse their impact on the indigenous plant-associated bacteria and fungi. lettuce seedlings were inoculated with bacterial suspensions of two endophytic strains, serratia plymuthica 3re4-18 and pseudomonas trivialis 3re2-7, and with the rhizobacterium pseudomonas fluorescens l13-6-12 7 days before and 5 days a ... | 2008 | 18248441 |
| thermally treated grass fibers as colonizable substrate for beneficial bacterial inoculum. | this study investigates how thermally treated (i.e., torrefied) grass, a new prospective ingredient of potting soils, is colonized by microorganisms. torrefied grass fibers (tgf) represent a specific colonizable niche, which is potentially useful to establish a beneficial microbial community that improves plant growth. tgf and torrefied grass extracts (tge) were inoculated with a suspension of microorganisms obtained from soil. sequential microbial enrichment steps were then performed in both su ... | 2008 | 18368438 |
| septic pseudarthrosis caused by serratia plymuthica. | | 2008 | 18455466 |
| phytase activity and its regulation in a rhizospheric strain of serratia plymuthica. | serratia plymuthica strain ic1270 isolated from the rhizosphere, possessing antagonistic activity towards a wide range of plant-pathogenic fungi, is able to hydrolyze phytate. phytase activity was found intracellularly, while no activity was detected in the culture liquid. optimum activity was found at ph 4-5; it completely disappeared at ph > 7.0 and 2.5. phytase production was practically absent in the exponential phase and reached a maximum in the late stationary phase. mutations of genes grr ... | 2008 | 18500629 |
| removal of phytotoxic compounds from torrefied grass fibres by plant-beneficial microorganisms. | we aimed to select microorganisms colonizing torrefied grass fibres (tgf) and simultaneously reducing the phytotoxicity which appeared after heat treatment of the fibres. eighty-eight bacterial strains and one fungus, previously isolated from a sequential enrichment experiment on torrefied fibres and extracts, were tested separately for their capacity to decrease phytotoxicity. eleven of the bacterial strains and the fungus significantly reduced phytotoxicity. these organisms were checked for th ... | 2008 | 18537835 |
| [influence of mutations in global regulator genes grrs and rpos on synthesis of phosphatases in serratia plymuthica]. | the participation of global regulators grrs (sensor kinase gaca/gacs-like regulatory system) and sigma s subunit of rna polymerase in the control of phosphatase synthesis in a soil bacterium serratia plymuthica was shown. in cells of null mutants for genes grrs and rpos synthesis of low-acidic and alkaline phosphatases was markedly decreased. | 2007 | 18592699 |
| a plasmid-transposon hybrid mutagenesis system effective in a broad range of enterobacteria. | random transposon mutagenesis is a powerful technique used to generate libraries of genetic insertions in many different bacterial strains. here we develop a system facilitating random transposon mutagenesis in a range of different gram-negative bacterial strains, including pectobacterium atrosepticum, citrobacter rodentium, serratia sp. atcc39006, serratia plymuthica, dickeya dadantii, and many more. transposon mutagenesis was optimized in each of these strains and three studies are presented t ... | 2015 | 26733980 |
| quorum-sensing effects in the antagonistic rhizosphere bacterium serratia plymuthica hro-c48. | the rhizosphere-associated bacterium serratia plymuthica hro-c48 is not only able to suppress symptoms caused by soil-borne pathogens but is also able to stimulate growth of plants. detailed knowledge about the underlying mechanisms and regulation are crucial for the application in biocontrol strategies. to analyse the influence of n-acyl homoserine lactone (ahl)-mediated communication on the biocontrol activity, the ahl-degrading lactonase aiia was heterologously expressed in the strain, result ... | 2009 | 19220861 |
| antibiotic susceptibility profiles of uncommon bacterial species causing severe infections in italy. | this study presents the results of the italian "severe infections project" involving bacteria that can be considered rare causes of disease. we isolated 30 uncommon human pathogens from a total of 60 strains (1.2% of all the isolates). the most frequent sources of uncommon human pathogens were primary bloodstream infections (48.3%) and pneumonia (20%). species such as comamonas testosteroni, enterococcus hirae, kluyvera ascorbata, kluyvera cryocrescens, leclercia adecarboxylata and ochrobactrum ... | 2009 | 19567344 |
| 2-amino-3-(oxirane-2,3-dicarboxamido)-propanoyl-valine, an effective peptide antibiotic from the epiphyte pantoea agglomerans 48b/90. | the epiphyte pantoea agglomerans 48b/90, which has been isolated from soybean leaves, belongs to the enterobacteriaceae, as does the plant pathogen erwinia amylovora, which causes fire blight on rosaceous plants such as apples and leads to severe economic losses. since p. agglomerans efficiently antagonizes phytopathogenic bacteria, the p. agglomerans strain c9-1 is used as a biocontrol agent (blightban c9-1). here we describe the bioassay-guided isolation of a peptide antibiotic that is highly ... | 2009 | 19820144 |
| isolation and taxonomic identity of bacteriocin-producing lactic acid bacteria from retail foods and animal sources. | bacteriocin-producing (bac⁺) lactic acid bacteria (lab) were isolated from a variety of food products and animal sources. samples were enriched in de man, rogosa, and sharpe (mrs) lactocilli broth and plated onto mrs agar plates using a "sandwich overlay" technique. inhibitory activity was detected by the "deferred antagonism" indicator overlay method using listeria monocytogenes as the primary indicator organism. antimicrobial activity against l. monocytogenes was detected by 41 isolates obtain ... | 2015 | 27682080 |
| prevalence and characterization of esbl- and ampc-producing enterobacteriaceae on retail vegetables. | in total 1216 vegetables obtained from dutch stores during 2012 and 2013 were analysed to determine the prevalence of 3rd-generation cephalosporin (3gc) resistant bacteria on soil-grown fresh produce possibly consumed raw. vegetables grown conventionally and organically, from dutch as well as foreign origin were compared. included were the following vegetable types; blanched celery (n=192), bunched carrots (n=190), butterhead lettuce (n=137), chicory (n=96), endive (n=188), iceberg lettuce (n=19 ... | 2015 | 25828704 |
| [involvement of the global regulators grrs, rpos, and splir in formation of biofilms in serratia plymuthica]. | most bacteria exist in the natural environment as biofilms, multicellular communities attached to hard surfaces. biofilms have a characteristic architecture and are enclosed in the exopolymer matrix. bacterial cells in biofilms are extremely resistant to antibacterial factors. it was shown in this work that the grra/grrs system of global regulators of gene expression and the sigma s subunit of rna polymerase (rpos) play a significant role in positive regulation of biofilm formation in the rhizos ... | 2010 | 20583596 |
| strain-specific colonization pattern of rhizoctonia antagonists in the root system of sugar beet. | to develop effective biocontrol strategies, basic knowledge of plant growth promotion (pgp) and root colonization by antagonists is essential. the survival and colonization patterns of five different biocontrol agents against rhizoctonia solani ag2-2iiib in the rhizosphere of greenhouse-grown sugar beet plants were analysed in single and combined treatments. the study included bacteria (pseudomonas fluorescens l13-6-12, pseudomonas trivialis re(*) 1-1-14, serratia plymuthica 3re4-18) as well as ... | 2010 | 20618857 |
| formate hydrogen lyase mediates stationary-phase deacidification and increases survival during sugar fermentation in acetoin-producing enterobacteria. | two fermentation types exist in the enterobacteriaceae family. mixed-acid fermenters produce substantial amounts of lactate, formate, acetate, and succinate, resulting in lethal medium acidification. on the other hand, 2,3-butanediol fermenters switch to the production of the neutral compounds acetoin and 2,3-butanediol and even deacidify the environment after an initial acidification phase, thereby avoiding cell death. we equipped three mixed-acid fermenters (salmonella typhimurium, s. enteriti ... | 2015 | 25762991 |
| broad-range antagonistic rhizobacteria pseudomonas fluorescens and serratia plymuthica suppress agrobacterium crown gall tumours on tomato plants. | to examine the biocontrol activity of broad-range antagonists serratia plymuthica ic1270, pseudomonas fluorescens q8r1-96 and p. fluorescens b-4117 against tumourigenic strains of agrobacterium tumefaciens and a. vitis. | 2010 | 21091861 |
| the effects of pollution on fish health. | potentially harmful substances-e.g. pesticides, heavy metals and hydrocarbons-are often released into the aquatic environment. when large quantities of pollutants are released there may be an immediate impact as measured by large-scale sudden mortalities of aquatic organisms, e.g. fish kills resulting from contamination of waterways with agricultural pesticides. lower levels of discharge may result in an accumulation of the pollutants in aquatic organisms. the end results, which may occur long a ... | 1998 | 21182713 |
| characterisation of two quorum sensing systems in the endophytic serratia plymuthica strain g3: differential control of motility and biofilm formation according to life-style. | n-acylhomoserine lactone (ahl)-based quorum sensing (qs) systems have been described in many plant-associated gram-negative bacteria to control certain beneficial phenotypic traits, such as production of biocontrol factors and plant growth promotion. however, the role of ahl-mediated signalling in the endophytic strains of plant-associated serratia is still poorly understood. an endophytic serratia sp. g3 with biocontrol potential and high levels of ahl signal production was isolated from the st ... | 2011 | 21284858 |
| integrated regulation of acetoin fermentation by quorum sensing and ph in serratia plymuthica rvh1. | during fermentation of sugars, a number of bacterial species are able to switch from mixed acid production to acetoin and 2,3-butanediol production in order to avoid lethal acidification of their environment, although the regulation of this switch is only poorly understood. in this study, we report the identification of the budab structural operon, involved in acetoin production in serratia plymuthica rvh1, and its activation by a lysr-type regulator encoded by budr, immediately upstream of this ... | 2011 | 21441339 |
| first report of serratia plymuthica causing onion bulb rot in poland. | specific bacterial disease symptoms were observed on onion bulbs in almost all regions in poland. for the purpose of identification of agents causing disease, bacteria were isolated from the symptomatic plants. their pathogenicity was confirmed by using pathogenicity test on onion scales. these bacteria were identified biochemically and molecularly as serratia plymuthica. | 2011 | 21630579 |
| microbial sucrose isomerases: producing organisms, genes and enzymes. | sucrose isomerase (si) activity is used industrially for the conversion of sucrose into isomers, particularly isomaltulose or trehalulose, which have properties advantageous over sucrose for some food uses. all of the known microbial sis are tim barrel proteins that convert sucrose without need for any cofactors, with varying kinetics and product specificities. the current analysis was undertaken to bridge key gaps between the information in patents and scientific publications about the microbes ... | 2012 | 22133441 |
| volatiles of two growth-inhibiting rhizobacteria commonly enroll atwrky18 function. | interactions with the (a)biotic environment play key roles for plant's fitness and vitality. in addition to direct surface-to-surface contact, volatile chemicals also affect other organisms. volatiles of serratia plymuthica and stenotrophomonas maltophilia significantly inhibited growth and induced h(2) o(2) production in arabidopsis in dual-culture. within one day, transcriptional changes could be observed by promoter-gus assays using a stress inducible, w-box containing 4xgst1 construct. expr ... | 2011 | 22188129 |
| microbial diversity inside pumpkins: microhabitat-specific communities display a high antagonistic potential against phytopathogens. | recent and substantial yield losses of styrian oil pumpkin (cucurbita pepo l. subsp. pepo var. styriaca greb.) are primarily caused by the ascomycetous fungus didymella bryoniae but bacterial pathogens are frequently involved as well. the diversity of endophytic microbial communities from seeds (spermosphere), roots (endorhiza), flowers (anthosphere), and fruits (carposphere) of three different pumpkin cultivars was studied to develop a biocontrol strategy. a multiphasic approach combining molec ... | 2011 | 21947430 |
| quorum-sensing quenching by rhizobacterial volatiles. | we show that volatile organic compounds (vocs) produced by rhizospheric strains pseudomonas fluorescens b-4117 and serratia plymuthica ic1270 may act as inhibitors of the cell-cell communication quorum-sensing (qs) network mediated by n-acyl homoserine lactone (ahl) signal molecules produced by various bacteria, including strains of agrobacterium, chromobacterium, pectobacterium and pseudomonas. this quorum-quenching effect was observed when ahl-producing bacteria were treated with vocs emitted ... | 2011 | 23761359 |
| a pks/nrps/fas hybrid gene cluster from serratia plymuthica rvh1 encoding the biosynthesis of three broad spectrum, zeamine-related antibiotics. | serratia plymuthica strain rvh1, initially isolated from an industrial food processing environment, displays potent antimicrobial activity towards a broad spectrum of gram-positive and gram-negative bacterial pathogens. isolation and subsequent structure determination of bioactive molecules led to the identification of two polyamino antibiotics with the same molecular structure as zeamine and zeamine ii as well as a third, closely related analogue, designated zeamine i. the gene cluster encoding ... | 2013 | 23349809 |
| volatile-mediated interactions between phylogenetically different soil bacteria. | there is increasing evidence that organic volatiles play an important role in interactions between micro-organisms in the porous soil matrix. here we report that volatile compounds emitted by different soil bacteria can affect the growth, antibiotic production and gene expression of the soil bacterium pseudomonas fluorescens pf0-1. we applied a novel cultivation approach that mimics the natural nutritional heterogeneity in soil in which p. fluorescens grown on nutrient-limited agar was exposed t ... | 2014 | 24966854 |
| effects of bacterial inoculants on the indigenous microbiome and secondary metabolites of chamomile plants. | plant-associated bacteria fulfill important functions for plant growth and health. however, our knowledge about the impact of bacterial treatments on the host's microbiome and physiology is limited. the present study was conducted to assess the impact of bacterial inoculants on the microbiome of chamomile plants chamomilla recutita (l.) rauschert grown in a field under organic management in egypt. chamomile seedlings were inoculated with three indigenous gram-positive strains (streptomyces subru ... | 2014 | 24600444 |
| transcriptomic changes in the plant pathogenic fungus rhizoctonia solani ag-3 in response to the antagonistic bacteria serratia proteamaculans and serratia plymuthica. | improved understanding of bacterial-fungal interactions in the rhizosphere should assist in the successful application of bacteria as biological control agents against fungal pathogens of plants, providing alternatives to chemicals in sustainable agriculture. rhizoctonia solani is an important soil-associated fungal pathogen and its chemical treatment is not feasible or economic. the genomes of the plant-associated bacteria serratia proteamaculans s4 and serratia plymuthica as13 have been sequen ... | 2015 | 26296338 |
| biosynthesis of the acetyl-coa carboxylase-inhibiting antibiotic, andrimid in serratia is regulated by hfq and the lysr-type transcriptional regulator, admx. | infections due to multidrug-resistant bacteria represent a major global health challenge. to combat this problem, new antibiotics are urgently needed and some plant-associated bacteria are a promising source. the rhizobacterium serratia plymuthica a153 produces several bioactive secondary metabolites, including the anti-oomycete and antifungal haterumalide, oocydin a and the broad spectrum polyamine antibiotic, zeamine. in this study, we show that a153 produces a second broad spectrum antibiotic ... | 2016 | 26914969 |
| [pleiotropic effects of chitinase gene from serratia plymuthica in transgenic potato]. | we estimate the influence of transgenic bacterial chitinase from soilborne serratia plymuthica onto agronomical important traits of potato, such as productivity and non-specific resistance. transgene has been delivered into potato variety delfin via agrobacterial transformation with pgreen0229 and pbi121-based vectors. growth inhibition of chitin-containing pathogenic fungi was shown. however, over 40% oftransgenic lines demonstrated decreased non-specific resistance to late blight (down to 62% ... | 2008 | 18630113 |
| genome sequence of serratia plymuthica strain s13, an endophyte with germination- and plant-growth-promoting activity from the flower of styrian oil pumpkin. | the bacterium serratia plymuthica strain s13 was demonstrated to colonize various plant-associated microhabitats and to suppress damping-off diseases. the completed genome sequence has a size of 5.5 mb, containing 4,957 putative protein-encoding regions, and will be used to identify genetic determinants enabling the bacterium to escort a plant's entire life cycle. | 2013 | 23929484 |
| draft genome sequence of the biocontrol strain serratia plymuthica a30, isolated from rotting potato tuber tissue. | serratia plymuthica a30 is a gram-negative bacterium expressing antagonistic activity toward blackleg- and soft rot-causing dickeya sp. biovar 3 ("dickeya solani"). here, we present the draft genome sequence of strain a30, which has been isolated from rotten potato tuber tissue. | 2012 | 23209245 |
| complete genome sequence of serratia plymuthica bacteriophage φmam1. | a virulent bacteriophage (φmam1) that infects serratia plymuthica was isolated from the natural environment and characterized. genomic sequence analysis revealed a circular double-stranded dna sequence of 157,834 bp, encoding 198 proteins and 3 trnas. the φmam1 genome shows high homology to previously reported vii-like enterobacterial bacteriophage genomes. | 2012 | 23166269 |
| quantitative and specific detection of the biocontrol agent, serratia plymuthica, in plant extracts using a real-time taqman® assay. | a serratia plymuthica-specific taqman® assay was designed based on the consensus nucleotide sequence from the 3'- end of the luxs gene present in all s. plymuthica strains tested. the specificity of the assay was demonstrated by testing 21 serratia spp. strains and 30 isolates belonging to various species that can potentially coexist with s. plymuthica in the same environment. positive reactions in the taqman® assay were observed only for s. plymuthica isolates and not for other bacteria. the ta ... | 2012 | 22886583 |
| draft genome sequence of the antagonistic rhizosphere bacterium serratia plymuthica strain pri-2c. | serratia plymuthica strain pri-2c is a rhizosphere bacterial strain with antagonistic activity against different plant pathogens. here we present the 5.39-mb (g+c content, 55.67%) draft genome sequence of s. plymuthica strain pri-2c with the aim of providing insight into the genomic basis of its antagonistic activity. | 2012 | 22815440 |
| bacterial-mediated induced resistance in cucumber: beneficial effect of the endophytic bacterium serratia plymuthica on the protection against infection by pythium ultimum. | abstract the potential of the endophytic bacterium serratia plymuthica strain r1gc4 in stimulating defense reactions in cucumber (cucumis sativus) seedlings inoculated with the soilborne pathogen pythium ultimum was explored at the cellular level. bacterial treatment prior to pythium inoculation resulted in less seedling disease development as compared with that in nontreated control plants, in which typical root symptoms were visible by 3 days after inoculation with the pathogen. histological i ... | 2000 | 18944571 |
| quorum sensing and butanediol fermentation affect colonization and spoilage of carrot slices by serratia plymuthica. | in this work we investigated the role of quorum sensing and specific quorum-sensing dependent properties in the colonization and spoilage of carrot slices by serratia plymuthica rvh1, a strain isolated previously from a vegetable washing and cutting machine in an industrial kitchen. disinfected carrot slices were inoculated by immersion in a bacterial suspension and then placed in a petri dish with a shallow layer of the same bacterial suspension. subsequently, visible spoilage of the air-expose ... | 2009 | 19162357 |
| analysis of a new cluster of genes involved in the synthesis of the unique volatile organic compound sodorifen of serratia plymuthica 4rx13. | the rhizobacterium serratia plymuthica 4rx13 emits the novel and unique volatile sodorifen (c16h26), which has a polymethylated bicyclic structure. transcriptome analysis revealed that gene sod_c20750 (annotated as terpene cyclase) is involved in the biosynthesis of sodorifen. here we show that this gene is located in a small cluster of four genes (sod_c20750 - sod_c20780), and the analysis of the knockout mutants demonstrated that sod_c20760 (annotated as methyltransferase) and sod_c20780 (anno ... | 2016 | 27231241 |
| effect of antibiotic supplementation on the quality of cryopreserved fish sperm of silver barb (barbodes gonionotus): sperm motility and viability, bacterial quality and fertilization. | disease prevention is a key aspect in developing cryopreservation procedures for fish sperm and in improving the reproductive biotechnology for commercially important aquatic species. the purpose of this study was to evaluate the effects of an antibiotic supplementation (0.25% penicillin-streptomycin, ps and 0.25% penicillin-gentamicin, pg) on sperm motility and viability, bacterial profile and fertilization capacity of cryopreserved silver barb (barbodes gonionotus) semen. the experimental prot ... | 2016 | 26786020 |
| influence of volatile organic compounds emitted by pseudomonas and serratia strains on agrobacterium tumefaciens biofilms. | the ability to form biofilms plays an important role in bacteria-host interactions, including plant pathogenicity. in this work, we investigated the action of volatile organic compounds (vocs) produced by rhizospheric strains of pseudomonas chlororaphis 449, pseudomonas fluorescens b-4117, serratia plymuthica ic1270, as well as serratia proteamaculans strain 94, isolated from spoiled meat, on biofilms formation by three strains of agrobacterium tumefaciens which are causative agents of crown-gal ... | 2016 | 27214244 |
| enhancing the thermostability of serratia plymuthica sucrose isomerase using b-factor-directed mutagenesis. | the sucrose isomerase of serratia plymuthica as9 (as9 pali) was expressed in escherichia coli bl21(de3) and characterized. the half-life of as9 pali was 20 min at 45°c, indicating that it was unstable. in order to improve its thermostability, six amino acid residues with higher b-factors were selected as targets for site-directed mutagenesis, and six mutants (e175n, k576d, k174d, g176d, s575d and n577k) were designed using the rosettadesign server. the e175n and k576d mutants exhibited improved ... | 2016 | 26886729 |
| rpos differentially affects the general stress response and biofilm formation in the endophytic serratia plymuthica g3. | the σ(s) subunit rpos of rna polymerase functions as a master regulator of the general stress response in escherichia coli and related bacteria. rpos has been reported to modulate biocontrol properties in the rhizobacterium serratia plymuthica ic1270. however, the role of rpos in the stress response and biofilm formation in s. plymuthica remains largely unknown. here we studied the role of rpos from an endophytic s. plymuthica g3 in regulating these phenotypes. mutational analysis demonstrated t ... | 2016 | 26671319 |
| acetoin synthesis acquisition favors escherichia coli growth at low ph. | some members of the family enterobacteriaceae ferment sugars via the mixed-acid fermentation pathway. this yields large amounts of acids, causing strong and sometimes even lethal acidification of the environment. other family members employ the 2,3-butanediol fermentation pathway, which generates comparatively less acidic and more neutral end products, such as acetoin and 2,3-butanediol. in this work, we equipped escherichia coli mg1655 with the budab operon, encoding the acetoin pathway, from s ... | 2014 | 25063653 |
| monoterpenes with antibacterial activities from a cameroonian medicinal plant canthium multiflorum (rubiaceae). | investigation of the crude extract obtained from the aerial parts of canthium multiflorum led to the isolation of a new iridoid (1) together with twelve known compounds. the structures of these compounds were elucidated by interpretation of 1d and 2d nmr spectroscopic data, accurate mass measurements and comparison with analytical data of previously known analogues. most of the isolated compounds have been reported for the first time from c. multiflorium. the antimicrobial activities of the isol ... | 2013 | 24028969 |
| microbial small talk: volatiles in fungal-bacterial interactions. | there is increasing evidence that volatile organic compounds (vocs) play an important role in the interactions between fungi and bacteria, two major groups of soil inhabiting microorganisms. yet, most of the research has been focused on effects of bacterial volatiles on suppression of plant pathogenic fungi whereas little is known about the responses of bacteria to fungal volatiles. in the current study we performed a metabolomics analysis of volatiles emitted by several fungal and oomycetal soi ... | 2015 | 26779150 |