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the specificity of bacterial siderophore receptors probed by bioassays.the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ...19911663779
the first isolate of tatumella ptyseos in malaysia.tatumella ptyseos, the type species for the genus tatumella, is a newly established member of the family enterobacteriaceae. it is a gram-negative, oxidase negative, fermentative rod that grows on mac conkey agar. this first isolate was obtained from the blood culture of a neonate having neonatal jaundice with presumed sepsis. the organism was in vitro sensitive to gentamicin, chloramphenicol, cotrimoxazole and ampicillin. the patient was treated with ampicillin and gentamicin and recovered unev ...19892632996
diversity of the phosphoenolpyruvate/glucose phosphotransferase system in the enterobacteriaceae.the presence of the phosphoenolpyruvate glucose phosphotransferase entry routes was studied in 97 genospecies of enterobacteriaceae. phosphoenolpyruvate(pep)-dependent phosphorylation of alpha-methyl-d-glucoside and 2-deoxyglucose was evidenced in 72 species (group i organisms), suggesting the presence of both the iiglc (formerly ii-bglc/iiiglc) and iiman (formerly ii-b/ii-aman) entry routes. erwinia amylovora, budvicia aquatica and all species of leminorella and proteus (as defined by dna relat ...19873606872
rapid catalase supplemental test for identification of members of the family enterobacteriaceae.a simple, rapid, semiquantitative slide catalase test useful for differentiating members of the family enterobacteriaceae is described. judging by the time required for appearance of oxygen bubbles in 3% hydrogen peroxide, the immediate catalase reactors were yersinia, serratia, proteus, morganella, providencia, cedecea, and hafnia spp. the delayed catalase reactors were escherichia, shigella, klebsiella, enterobacter, salmonella, citrobacter, edwardsiella, kluyvera, and tatumella spp. this info ...19873818934
tatumella ptyseos gen. nov., sp. nov., a member of the family enterobacteriaceae found in clinical specimens.the name tatumella ptyseos gen. nov., sp. nov., is proposed for a group of organisms (previously called group ef-9) isolated from clinical sources in the united states, canada, and puerto rico. a total of 68% of these isolates were from sputum specimens. t. ptyseos strains are gram-negative, oxidase-negative, fermentative rods that grow on macconkey agar. the distinctive biochemical characteristics of 44 t. ptyseos isolates were as follows: acid but no gas from d-glucose, sucrose, and, usually ( ...19817263854
distribution of diaminopropane and acetylspermidine in enterobacteriaceae.polyamines of 97 strains (60 species) belonging to 18 genera of the family enterobacteriaceae were determined by high performance liquid chromatographic analysis. in addition to putrescine and cadavarine, diaminopropane was widely distributed in enterobacteriaceae and almost ubiquitously within enterobacter, pantoea, erwinia, leminorella, proteus, leclercia, morganella, klebsiella, hafnia, rahnella, serratia, and tatumella species and sporadically within citrobacter, escherichia, moellerella, pr ...19968742354
phylogenetic analyses of klebsiella species delineate klebsiella and raoultella gen. nov., with description of raoultella ornithinolytica comb. nov., raoultella terrigena comb. nov. and raoultella planticola comb. nov.the phylogenetic relationships of the type strains of 9 klebsiella species and 20 species from 11 genera of the family enterobacteriaceae were investigated by performing a comparative analysis of the sequences of the 16s rrna and rpob genes. the sequence data were phylogenetically analysed by the neighbourjoining and parsimony methods. the phylogenetic inference of the sequence comparison confirmed that the genus klebsiella is heterogeneous and composed of species which form three clusters that ...200111411716
supercooling ability in two populations of the land snail helix pomatia (gastropoda: helicidae) and ice-nucleating activity of gut bacteria.the land snail helix pomatia (gastropoda: helicidae) is widely distributed in northern and central europe where it may experience subzero temperatures during winter months. its supercooling ability was studied in two populations of h. pomatia. one population originated from southern sweden (gotaland) and the other from central france (auvergne). in the experimental design, they were acclimated, over 2 weeks, to artificial winter conditions (hibernation, t=5 degrees c). the swedish snails showed ...200415710369
unusual stability of manganese superoxide dismutase from a new species, tatumella ptyseos ct: its gene structure, expression, and enzyme properties.a genomic dna of 1416 bp containing an open reading frame encoding a manganese superoxide dismutase (mn-sod) from tatumella ptyseos ct was cloned. sequence analysis of this new gene revealed that it translates 205 amino acid residues. the deduced amino acid sequence showed variable identities (41-91%) with sequences of mn-sods from other species. the residues required to coordinate the single trivalent manganese ion and the 11 residues putatively involved in the active center are conserved as th ...200515721770
phylogeny of the enterobacteriaceae based on genes encoding elongation factor tu and f-atpase beta-subunit.the phylogeny of enterobacterial species commonly found in clinical samples was analysed by comparing partial sequences of their elongation factor tu gene (tuf) and of their f-atpase beta-subunit gene (atpd). an 884 bp fragment for tuf and an 884 or 871 bp fragment for atpd were sequenced for 96 strains representing 78 species from 31 enterobacterial genera. the atpd sequence analysis exhibited an indel specific to pantoea and tatumella species, showing, for the first time, a tight phylogenetic ...200516166704
phylogeny and identification of pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (mlsa).species belonging to the genus of pantoea are commonly isolated from plants, humans and the natural environment. the species of the genus are phenotypically closely related, making rapid identification of pantoea strains to the species level difficult. multilocus sequence analysis (mlsa) was evaluated as a means for rapid classification and identification of pantoea strains. four housekeeping genes, gyrb, rpob, atpd and infb, were sequenced for strains assigned to the genus. included in the stud ...200819008066
tatumella ptyseos causing severe human infection: report of the first two brazilian cases.tatumella ptyseos is the type species of the tatumella genus (enterobacteriaceae). this fermentative gram-negative rod has only rarely been reported as a cause of human infections; there is very little information about it in the medical literature. we report here the first two brazilian cases of t. ptyseos infections, both evolving to severe sepsis.200819219286
transfer of pantoea citrea, pantoea punctata and pantoea terrea to the genus tatumella emend. as tatumella citrea comb. nov., tatumella punctata comb. nov. and tatumella terrea comb. nov. and description of tatumella morbirosei sp. nov.pantoea citrea, pantoea punctata and pantoea terrea were described for strains isolated from fruit and soil originating in japan. these three 'japanese' species have been shown to be phylogenetically distant from other species of the genus pantoea. it has been observed previously that, using multilocus sequence analysis (mlsa), the 'japanese' species consistently formed a distinct clade with an extended branch length, casting doubt on the inclusion of these species within the genus pantoea. furt ...201019654354
diversity of the total bacterial community associated with ghanaian and brazilian cocoa bean fermentation samples as revealed by a 16 s rrna gene clone library.cocoa bean fermentation is a spontaneous process involving a succession of microbial activities, starting with yeasts, followed by lactic acid bacteria and acetic acid bacteria. so far, all microbiological studies about cocoa bean fermentation were based on culture-dependent (isolation, cultivation, and identification), or, more recently, culture-independent (pcr-dgge, or polymerase chain reaction denaturing gradient gel electrophoresis) methods. using a metagenomic approach, total dna was extra ...201020559826
occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions.the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ...201021033566
bacterial species associated with sound and botrytis-infected grapes from a greek vineyard.grape bacterial microbiota plays central roles in the quality of grapes and wine, yet its diversity remains poorly described. in the present study, bacterial species associated with sound and botrytis-infected grapes of two cultivars originating from the same vineyard were assessed. isolates were identified by pcr-denaturing gradient gel electrophoresis (pcr-dgge) and sequence analysis of partial 16s rrna gene. comparable counts were recorded between botrytis-infected and sound grape samples. in ...201121315469
dynamics and species diversity of communities of lactic acid bacteria and acetic acid bacteria during spontaneous cocoa bean fermentation in vessels.to speed up research on the usefulness and selection of bacterial starter cultures for cocoa bean fermentation, a benchmark cocoa bean fermentation process under natural fermentation conditions was developed successfully. therefore, spontaneous fermentations of cocoa pulp-bean mass in vessels on a 20 kg scale were tried out in triplicate. the community dynamics and kinetics of these fermentations were studied through a multiphasic approach. microbiological analysis revealed a limited bacterial s ...201021356451
gram negative bacteria are associated with the early stages of necrotizing enterocolitis.necrotizing enterocolitis (nec) affects 5-10% of infants born weighing less than 1500 g. most models of nec recapitulate late-stage disease with gut necrosis and elevated inflammatory mediators. evaluation of nec at earlier, less lethal stages of disease will allow investigation of initial disease triggers and may advance our understanding of temporal relationships between factors implicated in nec pathogenesis. in this manuscript, we describe our investigation of early nec and test the hypothes ...201121445365
spontaneous organic cocoa bean box fermentations in brazil are characterized by a restricted species diversity of lactic acid bacteria and acetic acid bacteria.spontaneous organic cocoa bean box fermentations were carried out on two different farms in brazil. physical parameters, microbial growth, bacterial species diversity [mainly lactic acid bacteria (lab) and acetic acid bacteria (aab)], and metabolite kinetics were monitored, and chocolates were produced from the fermented dry cocoa beans. the main end-products of the catabolism of the pulp substrates (glucose, fructose, and citric acid) by yeasts, lab, and aab were ethanol, lactic acid, mannitol, ...201121839382
opportunistic pathogens relative to physicochemical factors in water storage tanks.household water in oman, as well as in other countries in the region, is stored in tanks placed on house roofs that can be subjected to physicochemical factors which can promote microbial growth, including pathogens and opportunistic pathogens which pose health risks. water samples were collected from 30 houses in a heavily populated suburb of muscat. the tanks used were either glass reinforced plastic (grp), polyethylene or galvanised iron (gi). heterotrophic bacteria, coliforms, faecal colifor ...201121942202
species diversity, community dynamics, and metabolite kinetics of the microbiota associated with traditional ecuadorian spontaneous cocoa bean fermentations.traditional fermentations of the local ecuadorian cocoa type nacional, with its fine flavor, are carried out in boxes and on platforms for a short time. a multiphasic approach, encompassing culture-dependent and -independent microbiological analyses of fermenting cocoa pulp-bean samples, metabolite target analyses of both cocoa pulp and beans, and sensory analysis of chocolates produced from the respective fermented dry beans, was applied for the investigation of the influence of these fermentat ...201121926224
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