| the taxonomic position of obesumbacterium proteus, a common brewery contaminant. | | 1973 | 4706971 |
| the construction and application of a lux-based nitrate biosensor. | a plasmid-borne transcriptional fusion between the escherichia coli nitrate reductase (narg) promoter and the photorhabdus luminescens lux operon provides e. coli with a highly bioluminescent phenotype in the presence of nitrate. this e. coli biosensor can detect nitrate to a level of 5 x 10(-5) mol l-1 (0.3 ppm), levels relevant to those levels encountered in brewing water. since induction of the narg promoter requires narl, the plasmid-based sensor can also be used to interrogate enteric bacte ... | 1997 | 9172442 |
| the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae. | the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ... | 1999 | 10555323 |
| rapid detection of obesumbacterium proteus from yeast and wort using polymerase chain reaction. | to design and evaluate pcr primers for the rapid detection of obesumbacterium proteus. | 2002 | 12358688 |
| gene cloning, expression and characterization of novel phytase from obesumbacterium proteus. | the gene phya encoding phytase was isolated from obesumbacterium proteus genomic library and sequenced. the cleavage site of the phya signal peptide was predicted and experimentally proved. the phya protein shows maximum identity of 53% and 47% to phosphoanhydride phosphorylase from yersinia pestis and phytase appa from escherichia coli, respectively. based on protein sequence similarity of phya and its homologs, the phytases form a novel subclass of the histidine acid phosphatase family. to cha ... | 2004 | 15251209 |
| biochemical and molecular characterization of obesumbacterium proteus, a common contaminant of brewing yeasts. | we have evaluated the effectiveness of api 20e, biolog testing, plasmid profiling, ribotyping, and enteric repetitive intergenic consensus (eric)-pcr to characterize, classify, and differentiate nine bacterial isolates of the common brewery contaminant obesumbacterium proteus. of the five typing techniques, biolog testing, plasmid profiling, and eric-pcr provided the most differentiation, and api 20e testing and ribotyping were relatively indiscriminate. the molecular biology approach of eric-pc ... | 1994 | 16349260 |
| characterization of the brewery spoilage bacterium obesumbacterium proteus by automated ribotyping and development of pcr methods for its biotype 1. | to study the ability of automated ribotyping to characterize obesumbacterium proteus and hafnia alvei, to design primers and to evaluate standard end-point and real-time pcr for the detection of o. proteus biotype 1 in beer and in brewers's yeast-containing samples. | 2006 | 16430517 |
| gram-negative bacteria associated with brewery yeasts: reclassification of obesumbacterium proteus biogroup 2 as shimwellia pseudoproteus gen. nov., sp. nov., and transfer of escherichia blattae to shimwellia blattae comb. nov. | phylogenetic analyses of type and reference strains of obesumbacterium proteus biogroups 1 and 2 plus a novel isolate of biogroup 2 were carried out based on 16s rrna gene sequences and partial sequences of four protein-coding genes (fusa, leus, pyrg and rpob). both approaches revealed that o. proteus biogroup 1 strains were closely related to hafnia alvei. biogroup 2 strains, however, formed a distinct monophyletic clade of generic status that included escherichia blattae. phenotypic tests were ... | 2010 | 19661513 |
| molecular analysis of intestinal microbiota of rainbow trout (oncorhynchus mykiss). | the aim of this study was to evaluate different molecular tools based on the 16s rrna gene, internal transcribed spacer, and the rpob gene to examine the bacterial populations present in juvenile rainbow trout intestines. dna was extracted from both pooled intestinal samples and bacterial strains. genes were pcr-amplified and analysed using both temporal temperature gradient gel electrophoresis (ttge) and restriction fragment length polymorphism methods. because of the high cultivability of the ... | 2010 | 19780831 |
| orbus hercynius gen. nov., sp. nov., isolated from faeces of wild boar, is most closely related to members of the orders 'enterobacteriales' and pasteurellales. | a novel gammaproteobacterium, strain cn3(t), was isolated from the faeces of wild boar. strain cn3(t) was facultatively anaerobic and appeared coccoid or rod-shaped. the partial 16s rrna gene sequence determined for strain cn3(t) suggested a distant relationship with members of the orders 'enterobacteriales' and pasteurellales. the gene sequence showed highest similarity (90.3 %) with obesumbacterium proteus dsm 2777(t), a member of the family enterobacteriaceae. the closest relatives outside th ... | 2010 | 20023064 |
| production of biogenic amines by lactic acid bacteria and enterobacteria isolated from fresh pork sausages packaged in different atmospheres and kept under refrigeration. | the occurrence of in vitro amino acid activity in bacterial strains associated with fresh pork sausages packaged in different atmospheres and kept in refrigeration was studied. the presence of biogenic amines in decarboxylase broth was confirmed by ion-exchange chromatography and by the presence of the corresponding decarboxylase genes by pcr. from the 93 lactic acid bacteria and 100 enterobacteria strains analysed, the decarboxylase medium underestimates the number of biogenic amine-producer st ... | 2011 | 21316866 |
| isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata). | the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ... | 2007 | 17657348 |
| the effect of decreasing alkalinity on microbial community dynamics in a sulfate-reducing bioreactor as analyzed by pcr-sscp. | pcr-single-strand conformation polymorphism (sscp) and southern blotting techniques were adopted to investigate microbial community dynamics in a sulfate-reducing bioreactor caused by decreasing influent alkalinity. experimental results indicated that the sulfate-removal rate approached 87% in 25 d under the conditions of influent alkalinity of 4000 mg/l (as caco3) and sulfate-loading rate of 4.8 g/(l*d), which indicated that the bioreactor started up successfully. the analysis of microbial comm ... | 2006 | 16989283 |