| [appendicitis: microbial interactions and new pathogens]. | the authors present an exhaustive review on microbial agents of appendicitis by means of literature and personal research data. thus, a detailed analysis is made on common autochthonous agents and their pathogenetic interactions and on less common exogenous bacterial, viral, mycotic, protozoan and helminthic agents with emphasis to the role of yersinia enterocolitica. in fact this bacterium seems responsible for 3% to 8% of cases in accordance with literature and personal research data (more det ... | 1992 | 1323137 |
| susceptibilities to antibiotics and antiseptics of new species of the family enterobacteriaceae. | one hundred and sixty-nine strains of new species of the family enterobacteriaceae, isolated mainly from the environment, were tested to determine their susceptibilities to 13 antibiotics and 4 antiseptics or disinfectants. all the species were susceptible to aminoglycosides, doxycycline, and trimethoprim but were resistant to chloramphenicol. susceptibility to beta-lactams varied more among the strains. however, all the strains were cefotaxime susceptible, apart from some buttiauxella agrestis ... | 1988 | 3415208 |
| isolation and properties of free and immobilized beta-galactosidase from the psychorotrophic enterobacterium buttiauxella agrestis (strain nc4). | a study of the beta-galactosidase produced by the psychrotrophic bacterium buttiauxella agrestis has been carried out. this micro-organism was isolated from raw milk and the enzyme isolated using standard methods. molecular mass was estimated to be 515 kda. the isoelectric point was close to 4.45. optimum ph was 7.25. maximal activity was observed at 50 degrees c and activation energy was estimated to be 39.1 kj mol-1. lactose enhanced thermal stability. using p-nitrophenyl-beta-d-galactopyranos ... | 1995 | 7615419 |
| the reduction of nitrous oxide to dinitrogen by escherichia coli. | escherichia coli k12 reduces nitrous oxide stoichiometrically to molecular nitrogen with rates of 1.9 mumol/h x mg protein. the activity is induced by anaerobiosis and nitrate. n2-formation from n2o is inhibited by c2h2 (ki approximately 0.03 mm in the medium) and nitrite (ki = 0.3 mm) but not by azide. a mutant defective in fnr synthesis is unable to reduce n2o to n2. the reaction in the wild type could routinely be followed by gas chromatography and alternatively by mass spectrometry measuring ... | 1993 | 8297209 |
| the phylogenetic position of serratia, buttiauxella and some other genera of the family enterobacteriaceae. | the phylogenetic relationships of the type strains of 38 species from 15 genera of the family enterobacteriaceae were investigated by comparative 16s rdna analysis. several sequences of strains from the genera citrobacter, erwinia, pantoea, proteus, rahnella and serratia, analysed in this study, have been analysed previously. however, as the sequences of this study differ slightly from the published ones, they were included in the analysis. of the 23 enterobacterial genera included in an overvie ... | 1999 | 10555323 |
| emended description of buttiauxella agrestis with recognition of six new species of buttiauxella and two new species of kluyvera: buttiauxella ferragutiae sp. nov., buttiauxella gaviniae sp. nov., buttiauxella brennerae sp. nov., buttiauxella izardii sp. nov., buttiauxella noackiae sp. nov., buttiauxella warmboldiae sp. nov., kluyvera cochleae sp. nov., and kluyvera georgiana sp. nov. | a total of 219 strains belonging to the genera buttiauxella and kluyvera were studied; 171 of these strains were isolated from mollusks, mainly snails and slugs, obtained from around the world. on the basis of dna-dna hybridization data, the strains were grouped into 11 genomospecies. a total of 44 phenotypic characters were used to differentiate the genera buttiauxella and kluyvera at the genus level and to identify genomospecies. there were significantly higher phenotypic probability distances ... | 1996 | 11534554 |
| but-1: a new member in the chromosomal inducible class c beta-lactamases family from a clinical isolate of buttiauxella sp. | an atypical enterobacteriaceae strain with a beta-lactam susceptibility pattern of inducible cephalosporinase was isolated in tenon hospital (paris, france) from a patient's skull wound infection. identifications by the api-50che biochemical system and 16s rrna gene sequencing concluded that it was a member of the buttiauxella genus. the bla gene was cloned and sequenced. the deduced translated product was a 383-amino acid protein (but-1) with 75-78% identity with the chromosomal ampc beta-lacta ... | 2002 | 12127496 |
| inducible ampc beta-lactamase of a new member enterobacteriaceae. | extensive biochemical testing and 16s rrna and rpob sequence analysis revealed that clinical strain cf01ent1, initially identified as buttiauxella agrestis by the use of api 32 biochemical strips, is a new organism in the enterobacteriaceae family. it produced an inducible ampc-type beta-lactamase whose sequence shares 69 to 72% identity with those of the other ampc-type beta-lactamases of enterobacteriaceae: this enzyme exhibits an atypical high affinity for all beta-lactams tested. | 2002 | 12234870 |
| isolation of buttiauxella gaviniae from a spinal cord patient with urinary bladder pathology. | a gram-negative buttiauxella gaviniae-like organism (lbv449) was isolated from a urine sample of a patient suffering from urine bladder pathology and neurological problems. the isolate was positive for adonitol fermentation and l-arginine dihydrolase and negative for melibiose and l-ornithine decarboxylase. the api 20e code was 3004113. retrospectively, another isolate (ent107), from a leg wound, was recovered from our collections and was shown to have similar biochemical characteristics. dna-dn ... | 2002 | 12354904 |
| supercooling ability in two populations of the land snail helix pomatia (gastropoda: helicidae) and ice-nucleating activity of gut bacteria. | the land snail helix pomatia (gastropoda: helicidae) is widely distributed in northern and central europe where it may experience subzero temperatures during winter months. its supercooling ability was studied in two populations of h. pomatia. one population originated from southern sweden (gotaland) and the other from central france (auvergne). in the experimental design, they were acclimated, over 2 weeks, to artificial winter conditions (hibernation, t=5 degrees c). the swedish snails showed ... | 2004 | 15710369 |
| phylogenetic analysis of enteric species of the family enterobacteriaceae using the oric-locus. | phylogenetic analysis of 38 enteric species belonging to the enterobacteraceae family was carried out using the non-coding locus oric, the chromosomal replication origin. the oric loci were amplified with conserved oligonucleotides and the pcr fragments were sequenced directly. the results establish a phylogenetic tree for the classification of different species of the genera escherichia, shigella, salmonella, enterobacter, citrobacter, klebsiella, raoultella, kluyvera, cedecea and buttiauxella. ... | 2007 | 16904857 |
| isolation and characterization of cold-adapted strains producing beta-galactosidase. | beta-galactosidase is extensively employed in the manufacture of dairy products, including lactose-reduced milk. here, we have isolated two gram-negative and rod-shaped coldadapted bacteria, bs 1 and hs 39. these strains were able to break down lactose at low temperatures. although two isolates were found to grow well at 10 degrees , the bs 1 strain was unable to grow at 37 degrees . another strain, hs-39, evidenced retarded growth at 37 degrees . the biochemical characteristics and the results ... | 2006 | 16953174 |
| the survival of pathogens in soil treated with wastewater sludge and in potatoes grown in such soil. | the prevalence of pathogens on potatoes (solanum tuberosum) grown in soil amended with a pathogen rich wastewater sludge was investigated. bacteria of the family enterobacteriaceae are important pathogens causing intestinal and systemic illness of humans and other animals. type b sludge was used. sludges investigated are the high metal and the low metal sludges. microorganisms in the sludge-amended soil were using culture-based technique. salmonella and e. coli were observed in tested soil sampl ... | 2006 | 17087382 |
| comparison of disk diffusion, vitek 2, and broth microdilution antimicrobial susceptibility test results for unusual species of enterobacteriaceae. | we compared the antimicrobial susceptibility testing results generated by disk diffusion and the vitek 2 automated system with the results of the clinical and laboratory standards institute (clsi) broth microdilution (bmd) reference method for 61 isolates of unusual species of enterobacteriaceae. the isolates represented 15 genera and 26 different species, including buttiauxella, cedecea, kluyvera, leminorella, and yokenella. antimicrobial agents included aminoglycosides, carbapenems, cephalospo ... | 2007 | 17135429 |
| isolation and characterization of cultivable fermentative bacteria from the intestine of two edible snails, helixpomatia and cornu aspersum (gastropoda: pulmonata). | the intestinal microbiota of the edible snails cornu aspersum fsyn: h. aspersa), and helix pomatia were investigated by culture-based methods, 16s rrna sequence analyses and phenotypic characterisations. the study was carried out on aestivating snails and two populations of h. pomatia were considered. the cultivable bacteria dominated in the distal part of the intestine, with up to 5.10(9) cfu g -1, but the swedish h. pomatia appeared significantly less colonised, suggesting a higher sensitivity ... | 2007 | 17657348 |
| mesophilic and psychrotrophic bacteria from meat and their spoilage potential in vitro and in beef. | mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (voc) by gas chromatography-mass spectrometry (gc/ms). fifty mesophilic and twenty-nine psychrotrophic isolates were analyzed by random amplified polymorphic dna-pcr, and representative strains were identified by 16s rrna gene sequencing. carnobacterium maltaromaticum ... | 2009 | 19201980 |
| molecular analysis of spoilage-related bacteria in pasteurized milk during refrigeration by pcr and denaturing gradient gel electrophoresis. | bacterial diversity in fluid milk products has been extensively studied in order to improve milk quality. here, we illustrate the utility of viable counts and pcr-denaturing gradient gel electrophoresis (dgge) for monitoring the microbial spoilage of pasteurized milk during shelf life. five pasteurized milk samples stored at 4 degrees c were examined at 10 and 5 days before expiration and on the expiration day. with bacterial dna extracted directly from the samples, pcr-dgge analysis indicated t ... | 2009 | 19343946 |
| characterization of the steroid degrading bacterium s19-1 from the baltic sea at kiel, germany. | steroid contamination of sea water is an ever growing problem and impacts population dynamics of all kinds of sea animals. we have long experience with the soil bacterium comamonas testosteroni atcc11996 which is able to catabolize a variety of steroids and polycyclic aromatic hydrocarbons, and which might be used in the bioremediation of contaminated places. in c. testosteroni about 20 enzymes which are involved in steroid degradation could be induced by 0.5mm testosterone. in this work, we iso ... | 2010 | 21185812 |
| diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis. | microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ... | 2011 | 21660545 |
| microbial community composition and reactor performance during hydrogen production in a uasb reactor fed with raw cheese whey inoculated with compost. | this study investigated the microbial community developed in a uasb reactor for hydrogen production and correlated it to reactor performance. the reactor was inoculated with kitchen waste compost and fed with raw cheese whey at two organic loading rates, 20 gcod/ld and 30 gcod/ld. hydrogen production was very variable, using an olr of 30 gcod/ld averaged 1.0 lh(2)/ld with no methane produced under these conditions. the hydrogen yield was also very variable and far from the theoretical. this low ... | 2011 | 22156132 |