| cholesterol beta-d-glucoside-6'-o-palmitate, a metabolite of pythium sylvaticum. | cholesterol beta-d-glucoside-6'-o-palmitate has been identified as a polar metabolite in the mycelium of mated cultures of pythium sylvaticum grown in the presence of cholesterol. the structure was confirmed by synthesis of the metabolite. similar steroid beta-d-glucoside-6'-o-palmitates were obtained from beta-sitosterol and campesterol when these sterols were added to cultures of p-sylvaticum. corresponding esters of myristic and stearic acids were also detected. | 1977 | 836860 |
| sexuality in pythium sylvaticum: heterothallism. | | 1967 | 6042863 |
| meiotic instability of pythium sylvaticum as demonstrated by inheritance of nuclear markers and karyotype analysis. | progeny from a sexual outcross between opposite mating types of pythium sylvaticum were analyzed for inheritance of rflp and random amplified polymorphic dna (rapd) markers. although most were inherited in expected mendelian frequencies, several were not. pulsed field gel electrophoresis was employed to examine these unexpected patterns of marker inheritance at a karyotypic level. parental oogonial and antheridial isolates had different electrophoretic karyotypes and minimum number of chromosome ... | 1995 | 7768436 |
| purification, crystallization and preliminary x-ray studies of sylvaticin, an elicitin-like protein from pythium sylvaticum. | sylvaticin belongs to the elicitin family. these 10 kda oomycetous proteins induce a hypersensitive response in plants, including necrosis and cell death, but subsequently leading to a non-specific systemic acquired resistance (sar) against other pathogens. sylvaticin has been crystallized using peg 2000 mme as a precipitant agent in the presence of nickel chloride. the crystals belong to space group c2, with unit-cell parameters a = 99.29, b = 25.67, c = 67.45 a, beta = 99.66 degrees. diffracti ... | 2004 | 14747726 |
| universally occurring phenylpropanoid and species-specific indolic metabolites in infected and uninfected arabidopsis thaliana roots and leaves. | a total of eleven alkali-released, aromatic compounds were identified by hplc, ms and nmr analyses in cell wall extracts from arabidopsis thaliana roots. nine of them together constituted the three complete series of 4-hydroxy-, 4-hydroxy-3-methoxy, and 4-hydroxy-3,5-dimethoxy-substituted benzaldehydes, benzoic acids and cinnamic acids. the other two were indolic metabolites: indole-3-carboxylic acid and indole-3-carbaldehyde. qualitatively similar, but quantitatively distinct profiles were obta ... | 2004 | 15016565 |
| structural complexity, differential response to infection, and tissue specificity of indolic and phenylpropanoid secondary metabolism in arabidopsis roots. | levels of indolic and phenylpropanoid secondary metabolites in arabidopsis (arabidopsis thaliana) leaves undergo rapid and drastic changes during pathogen defense, yet little is known about this process in roots. using arabidopsis wild-type and mutant root cultures as an experimental system, and the root-pathogenic oomycete, pythium sylvaticum, for infections, we analyzed the aromatic metabolite profiles in soluble extracts from uninfected and infected roots, as well as from the surrounding medi ... | 2005 | 15923335 |
| a new species of pythium with ornamented oogonia: morphology, taxonomy, internal transcribed spacer region of its ribosomal rna, and its comparison with related species. | pythium spiculum sp. nov. was isolated from soil samples taken in a vineyard in the burgundian region of france and from different locations in spain and portugal. the oomycete has spiny oogonia and does not sporulate readily. it resembles pythium mamillatum meurs, but has its own distinguishing characteristics. it also exhibits sickle-shaped as well as spherical appressoria which at times are associated with sex organs like those found in pythium abappressorium paulitz and pythium contiguanum p ... | 2006 | 16445762 |
| differences in microbial activity and microbial populations of peat associated with suppression of damping-off disease caused by pythium sylvaticum. | the microbiological characteristics associated with disease-suppressive peats are unclear. we used a bioassay for pythium sylvaticum-induced damping-off of cress seedlings to identify conducive and suppressive peats. microbial activity in unconditioned peats was negatively correlated with the counts of p. sylvaticum at the end of the bioassay. denaturing gradient gel electrophoresis (dgge) profiling and clone library analyses of small-subunit rrna gene sequences from two suppressive and two cond ... | 2006 | 17021192 |
| structure of sylvaticin, a new alpha-elicitin-like protein from pythium sylvaticum. | the structure of sylvaticin, a 10 kda major pythin protein excreted by the parasitic oomycete pythium sylvaticum, has been determined. although closely related to alpha-elicitins in its biological response, toxicity and overall structure, sylvaticin presents a number of structural features that make it an unusual member of the elicitin class. elicitins possess a large hydrophobic cavity and the mechanism of the systemic acquired resistance induced in planta is known to proceed through lipid tran ... | 2007 | 17881828 |
| selection of drug-tolerant strains of pythium sylvaticum using sublethal enrichment. | abstract sublethal enrichment was used to generate mutants of pythium sylvaticum tolerant to kanamycin and tetracycline. kanamycin tolerance was readily generated, and mutants had growth rates similar to wild-type isolates at antibiotic concentrations lethal to wild-type isolates. based on crosses between wild-type and mutant isolates, two types of inheritance of tolerance were identified. a high level of kanamycin tolerance was inherited in progeny only when the maternal parent was drug toleran ... | 1997 | 18945089 |