Publications

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use of polymerase chain reaction to detect the soft rot pathogen, pythium myriotylum, in infected ginger rhizomes.the aims are to establish a polymerase chain reaction (pcr)-based method for detecting pythium myriotylum in the rhizome of ginger and diagnosing ginger soft rot and screening health seed ginger.200312535133
detection of the low-germination-rate resting oospores of pythium myriotylum from soil by pcr.to establish a sensitive and specific polymerase chain reaction (pcr)-based method for detecting pythium myriotylum in soils.200312581375
characterization of cmr5c and cmr12a, novel fluorescent pseudomonas strains from the cocoyam rhizosphere with biocontrol activity.to screen for novel antagonistic pseudomonas strains producing both phenazines and biosurfactants that are as effective as pseudomonas aeruginosa pna1 in the biocontrol of cocoyam root rot caused by pythium myriotylum.200717897205
phenazines and biosurfactants interact in the biological control of soil-borne diseases caused by pythium spp.in this study, the putative role of phenazines and rhamnolipid-biosurfactants, antagonistic metabolites produced by pseudomonas aeruginosa pna1, was tested in the biological control of pythium splendens on bean (phaseolus vulgaris l) and pythium myriotylum on cocoyam (xanthosoma sagittifolium l schott). a rhamnolipid-deficient and a phenazine-deficient mutant of pna1 were used either separately or jointly in plant experiments. when the mutants were applied separately, no disease-suppressive effe ...200818237310
beauveria bassiana: endophytic colonization and plant disease control.seed application of beauveria bassiana 11-98 resulted in endophytic colonization of tomato and cotton seedlings and protection against plant pathogenic rhizoctonia solani and pythium myriotylum. both pathogens cause damping off of seedlings and root rot of older plants. the degree of disease control achieved depended upon the population density of b. bassiana conidia on seed. using standard plating techniques onto selective medium, endophytic 11-98 was recovered from surface-sterilized roots, st ...200818442830
use of cross-flow membrane filtration in a recirculating hydroponic system to suppress root disease in pepper caused by pythium myriotylum.zoosporic pathogens in the genera pythium and phytophthora cause extensive root disease epiphytotics in recirculating hydroponic vegetable-production greenhouses. zoospore cysts of pythium myriotylum drechsler were used to evaluate the effectiveness of cross-flow membrane filters to control pythiaceous pathogens in recirculating hydroponic systems. four membrane filter brands (honeycomb, polypure, polymate, and absolife) were tested alone or in combination to determine which filters would effect ...200919351256
identification and characterization of bacterial endophytes of rice.we isolated seven different bacteria from rice seedlings grown from surface sterilized seeds. three were associated with the rice seed husk and the other four were growing endophytically within the seed. microscopic studies revealed that the endophytes were concentrated in the root stele region. some of the bacteria exhibited strong anti-fungal activity against rhizoctonia solani, pythium myriotylum, guamannomyces graminis and heterobasidium annosum.199620882464
a pcr-rflp assay for identification and detection of pythium myriotylum, causal agent of the cocoyam root rot disease.development of a pcr-rflp assay that could reliably distinguish strains of pythium myriotylum that are pathogenic to cocoyam from nonpathogens, as well as in planta detection of the pathogen.201121204883
n-acylhomoserine lactone quorum-sensing signalling in antagonistic phenazine-producing pseudomonas isolates from the red cocoyam rhizosphere.forty fluorescent pseudomonas strains isolated from white and red cocoyam roots were tested for their ability to synthesize n-acyl-l-homoserine lactones (acyl-hsls). remarkably, only isolates from the red cocoyam rhizosphere that were antagonistic against the cocoyam root rot pathogen pythium myriotylum and synthesized phenazine antibiotics produced acyl-hsls. this supports the assumption that acyl-hsl production is related to the antagonistic activity of the strains. after detection, the signal ...201021071496
loss of pectin is an early event during infection of cocoyam roots by pythium myriotylum.cocoyam (xanthosoma sagittifolium) is an important tuber crop in most tropical zones of africa and america. in cameroon, its cultivation is hampered by a soil-borne fungus pythium myriotylum which is responsible for root rot disease. the mechanism of root colonisation by the fungus has yet to be elucidated. in this study, using microscopical and immunocytochemical methods, we provide a new evidence regarding the mode of action of the fungus and we describe the reaction of the plant to the early ...200616160840
intraspecific variability of pythium myriotylum isolated from cocoyam and other host crops.intraspecific variability within 51 isolates of pythium myriotylum from cocoyam (xanthosoma sagittifolium) and other host crops was analysed using optimum growth temperature, esterase banding patterns, aflps, rdna-its sequencing, and virulence to cocoyam. p. myriotylum isolates virulent to cocoyam could easily be differentiated from other isolates of p. myriotylum by their optimum growth temperature. isolates from cocoyam grew best at 28 degrees c with no growth at 37 degrees c, while p. myrioty ...200616546366
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