| peritonitis with cdc group ivc-2 bacteria in a patient on continuous ambulatory peritoneal dialysis. | a case of cdc group ivc-2 peritonitis in a patient on continuous ambulatory peritoneal dialysis (capd) is described. to the authors' knowledge, this is the first case reported of capd peritonitis in which a member of this unusual group of bacteria was isolated as the sole microorganism. as this microorganism is usually resistant to most antibiotics commonly used to treat peritonitis in patients on capd, microbiological investigations with identification and antimicrobial susceptibility tests are ... | 1991 | 1915387 |
| human infection due to the cdc group ivc-2 bacterium: case report and review. | | 1994 | 8011848 |
| distribution of the catabolic transposon tn5271 in a groundwater bioremediation system. | the distribution of tn5271-related dna sequences in samples of groundwater and a groundwater bioremediation system at the hyde park (niagara falls, n.y.) chemical landfill site was investigated. pcr amplification of target sequences within the cha genes of tn5271 revealed similar sequences in the groundwater community and in samples from the sequencing batch reactors treating that groundwater. cell dilution combined with pcr amplification indicated that cha sequences were carried in about 1 of 1 ... | 1994 | 8117095 |
| recurrent cdc group ivc-2 bacteremia in a human with aids. | a 30-year-old man with aids developed separate episodes of bacteremia with multiple organisms including cdc group ivc-2, related to an indwelling central venous catheter. recovery at each interval followed antibiotic therapy and replacement of the central venous access. this is the first reported case of infection with this organism in a patient with aids. a review of the biochemical features of this bacterium as well as pertinent literature is presented. | 1997 | 9041435 |
| phenotypic and genotypic characterization of clinical strains of cdc group ivc-2. | cdc group ivc-2 is a gram-negative, oxidase-positive, nonfermentative bacillus that has been implicated in human infections, including septicemia and peritonitis. biochemically it most closely resembles bordetella bronchiseptica and alcaligenes sp. results of cellular fatty acid (cfa) and 16s rrna gene analysis were combined with biochemical data to assist in identification and classification. the predominant cfas were hexadecanoic acid (16:0), cis-9-hexadecanoic acid (16:1omega7c), cis-11-octad ... | 1998 | 9705403 |
| assignment of centers for disease control group ivc-2 to the genus ralstonia as ralstonia paucula sp. nov. | an integrated genotypic and phenotypic analysis of 12 centers for disease control (cdc) group ivc-2 strains revealed that this taxon represents a novel species belonging to the genus ralstonia. comparative 16s rdna sequence analysis allocated a representative cdc group ivc-2 strain to the ralstonia branch of the beta subclass of the proteobacteria. dna-dna hybridizations did not detect significant binding levels towards any presently known ralstonia species, including ralstonia pickettii. its dn ... | 1999 | 10319489 |
| description of pandoraea gen. nov. with pandoraea apista sp. nov., pandoraea pulmonicola sp. nov., pandoraea pnomenusa sp. nov., pandoraea sputorum sp. nov. and pandoraea norimbergensis comb. nov. | a polyphasic taxonomic study was performed on a group of isolates tentatively identified as burkholderia cepacia, ralstonia pickettii or ralstonia paucula (formerly known as cdc group ivc-2). the isolates were mainly cultured from sputum of cystic fibrosis patients or from soil. sds-page of whole-cell proteins and aflp fingerprinting distinguished at least five different species, and this was confirmed by dna-dna hybridizations. 16s rdna sequence analysis of representative strains indicated that ... | 2000 | 10758901 |
| ralstonia paucula (formerly cdc group iv c-2): unsuccessful strain differentiation with pcr-based methods, study of the 16s-23s spacer of the rrna operon, and comparison with other ralstonia species (r. eutropha, r. pickettii, r. gilardii, and r. solanacearum). | ralstonia paucula (formerly cdc group iv c-2) can cause serious human infections. confronted in 1995 with five cases of nosocomial bacteremia, we found that pulsed-field gel electrophoresis could not distinguish between the isolates and that randomly amplified polymorphic dna analysis was poorly discriminatory. in this study, we used pcr-ribotyping and pcr-restriction fragment length polymorphism analysis of the spacer 16s-23s ribosomal dna (rdna); both methods were unable to differentiate r. pa ... | 2001 | 11136807 |
| identification of strains of alcaligenes and agrobacterium by a polyphasic approach. | the number of stable discriminant biochemical characters is limited in the genera alcaligenes and agrobacterium, whose species are consequently difficult to distinguish from one another by conventional tests. moreover, genomic studies have recently drastically modified the nomenclature of these genera; for example, alcaligenes xylosoxidans was transferred to the genus achromobacter in 1998. twenty-five strains of achromobacter xylosoxidans, three strains of an agrobacterium sp., five strains of ... | 2001 | 11526136 |
| comparative evaluation of the bd phoenix and vitek 2 automated instruments for identification of isolates of the burkholderia cepacia complex. | we evaluated two new automated identification systems, the bd phoenix (becton dickinson) and the vitek 2 (biomérieux), for identification of isolates of the burkholderia cepacia complex (bcc). the test sample included 42 isolates of the highly virulent and epidemic genomovar iii, 45 isolates of b. multivorans, and 47 isolates of other members of the bcc. rates of correct identification by the bd phoenix and vitek 2 were similar when all bcc isolates were considered (50 and 53%, respectively) but ... | 2002 | 11980954 |
| dna fingerprinting of ralstonia paucula by infrequent-restriction-site pcr and randomly amplified polymorphic dna analysis. | ralstonia paucula (formerly cdc group iv c-2) is an environmental organism that can cause serious human infections, occasionally clusters of nosocomial infections. in the present work, 26 strains of r. paucula (4 from the american centers for disease control and prevention collection, 10 from the belgian laboratorium voor microbiologie [lmg] collection, and 12 french clinical isolates) were analyzed with infrequent-restriction-site pcr and randomly amplified polymorphic dna analysis. both techni ... | 2003 | 14662974 |
| wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including ralstonia eutropha and related species, and proposal of ralstonia [pseudomonas] syzygii (roberts et al. 1990) comb. nov. | comparative 16s rdna sequence analysis indicates that two distinct sublineages, with a sequence dissimilarity of >4 % (bootstrap value, 100 %), exist within the genus ralstonia: the ralstonia eutropha lineage, which comprises ralstonia basilensis, ralstonia campinensis, r. eutropha, ralstonia gilardii, ralstonia metallidurans, ralstonia oxalatica, ralstonia paucula, ralstonia respiraculi and ralstonia taiwanensis; and the ralstonia pickettii lineage, which comprises ralstonia insidiosa, ralstoni ... | 2004 | 15023939 |
| taxonomy of the genus cupriavidus: a tale of lost and found. | dna-dna hybridization experiments and an evaluation of phenotypic characteristics, dna base ratios and 16s rrna gene sequences demonstrated that wautersia eutropha (davies 1969) vaneechoutte et al. 2004, the type species of the genus wautersia, is a later synonym of cupriavidus necator makkar and casida 1987, the type species of the genus cupriavidus. in conformity with rules 15, 17, 23a and 37a(1) of the international code of nomenclature of bacteria, the genus name cupriavidus has priority ove ... | 2004 | 15545472 |
| investigation of product quality between extemporaneously compounded progesterone vaginal suppositories and an approved progesterone vaginal gel. | the purpose of this investigation was to compare quality parameters, including product appearance, content uniformity, ph, weight uniformity, microbial limit testing and preservative effectiveness testing on extemporaneously compounded progesterone vaginal suppositories obtained from 10 randomly chosen compounding pharmacies (90 suppositories each) across the united states, to the food and drug administration (fda) approved prescription progesterone gel product (prochieve/crinone) which is manuf ... | 2004 | 15595573 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| [cupriavidus pauculus isolation in the intensive care unit]. | | 2008 | 18588825 |
| pseudo-outbreak of cupriavidus pauculus infection at an outpatient clinic related to rinsing culturette swabs in tap water. | cupriavidus pauculus is a water microorganism rarely isolated from clinical specimens. we describe a pseudo-outbreak in which multiple strains that were associated with moistening of culturette swabs with tap water were isolated from a single clinic before collecting the patient specimen. | 2010 | 20444965 |
| [a case of ventilator-associated pneumonia caused by cupriavidus pauculus]. | cupriavidus pauculus (formerly cdc group ivc-2) is a non-fermentative, motile, gram-negative bacillus, rarely associated with human infections. it has been isolated from water, water from ultrafiltration systems and bottled mineral water. to date, 19 cases of bacteremia, two cases of peritonitis and one case of tenosynovitis associated with c. pauculus have been reported in english literature. in this paper, we report the first case of c. pauculus ventilator-associated pneumonia (vap) in turkey. ... | 2010 | 20455409 |
| efficacy of various chemical disinfectants on biofilms formed in spacecraft potable water system components. | as the provision of potable water is critical for successful habitation of the international space station (iss), life support systems were installed in december 2008 to recycle both humidity from the atmosphere and urine to conserve available water in the station. in-flight pre-consumption testing from the dispensing needle at the potable water dispenser (pwd) indicated that bacterial concentrations exceeded the current iss specifications of 50 colony-forming units (cfu) ml(-1). subsequent inve ... | 2010 | 20544435 |
| nosocomial transmission of cupriavidus pauculus during extracorporeal membrane oxygenation. | patients undergoing extracorporeal membrane oxygenation (ecmo) are at increased risk of infection. we present the first known report of nosocomial infection with cupriavidus pauculus attributable to contamination from ecmo equipment and describe the measures taken to halt subsequent infections. a cluster of infections in ecmo patients should prompt team members to consider contamination of equipment with environmental pathogens as a possible cause. | 2010 | 20811172 |
| outbreak of ralstonia paucula pseudobacteraemia in a paediatric accident and emergency department. | | 2011 | 21440953 |
| analysis of the bacterial diversity existing on animal hide and wool: development of a preliminary pcr-restriction fragment length polymorphism fingerprint database for identifying isolates. | twenty-one bacterial strains were isolated from imported cattle hide and rabbit wool using two types of media, nutrient broth, and nutrient broth with serum. the bacteria identified were brevibacillus laterosporus, leclercia adecarboxylata, peptococcus niger, bacillus circulans, raoultella ornithinolytica, bacillus subtilis, bacillus cereus, bacillus thermobacillus, bacillus choshinensis, bacillus sphaericus, acinetobacter haemolyticus, sphingomonas paucimobilis, bacillus thuringiensis, staphylo ... | 2012 | 23451394 |
| pyruvic oxime nitrification and copper and nickel resistance by a cupriavidus pauculus, an active heterotrophic nitrifier-denitrifier. | heterotrophic nitrifiers synthesize nitrogenous gasses when nitrifying ammonium ion. a cupriavidus pauculus, previously thought an alcaligenes sp. and noted as an active heterotrophic nitrifier-denitrifier, was examined for its ability to produce nitrogen gas (n2) and nitrous oxide (n2o) while heterotrophically nitrifying the organic substrate pyruvic oxime [ch3-c(noh)-cooh]. neither n2 nor n2o were produced. nucleotide and phylogenetic analyses indicated that the organism is a member of a genus ... | 2014 | 25580463 |
| draft genome sequence of cupriavidus pauculus strain kf709, a biphenyl-utilizing bacterium isolated from biphenyl-contaminated soil. | we report the draft genome sequence of cupriavidus pauculus strain kf709, which comprises 6,826,799 bp with 6,272 coding sequences. the strain kf709 utilizes biphenyl and degrades low-chlorinated biphenyls; however, it possesses fewer coding sequences involved in the degradation of aromatic compounds than other strains belonging to the betaproteobacteria. | 2015 | 25814614 |
| clinical characteristics of ochrobactrum anthropi bacteremia. | the clinical picture of ochrobactrum anthropi infection is not well described because the infection is rare in humans and identification of the pathogen is difficult. we present a case of o. anthropi bacteremia that was initially misidentified as ralstonia paucula and later identified by 16s rrna sequencing and reca analysis. | 2013 | 23363833 |
| micro-organisms associated with febrile neutropenia in patients with haematological malignancies in a tertiary care hospital in eastern india. | there is paucity of information from eastern india with regard to observed dominant micro-organisms causing febrile neutropenia (fn) in patients with haematological malignancies. to identify the prevalence of pathogenic microorganisms associated with fn. a total number of 268 episodes of fn were analysed from september'2010 to october'2013. the blood samples were inoculated into brain heart infusion broth, glucose broth, hicombi dual performance media (himedia, lq-12) at 37° c for 168 h and bact ... | 2014 | 25548444 |
| first case of pneumonia caused by cupriavidus pauculus in an infant in the gulf cooperation council. | cupriavidus pauculus is an emerging organism causing infections in immunocompromised and immunocompetent patients. we report a c.pauculus pneumonia case susceptible to cefepime in an infant with end-stage renal failure. to our knowledge, this is the first case report of c. pauculus causing respiratory infections in the gulf cooperation council. | 2017 | 28248683 |
| cupriavidus pauculus bacteremia in a child on extracorporeal membrane oxygenation. | we report a case of bacteremia secondary to cupriavidus pauculus in a 15-month-old boy on extracorporeal membrane oxygenation (ecmo). the source of the organism was water in the thermoregulator reservoir. the child responded well to cefepime and ciprofloxacin, a delayed oxygenator change out and replacement of the thermoregulator reservoir with a unit that was cleaned and decontaminated with sodium hypochlorite. isolation of cupriavidus pauculus from a patient on ecmo support should raise suspic ... | 2014 | 25072551 |
| cupriavidus pauculus (ralstonia paucula) concomitant meningitis and septicemia in a neonate: first case report from india. | ralstonia paucula (formerly classified as cdc (centre for disease control) group ivc-2, wautersia paucula; recently renamed as cupriavidus pauculus) is an environmental gram-negative bacillus isolated from water sources and can cause serious human infections. patients recover bacteriologically indicating low virulence. a total of 32 cases have been reported world-wide, but no isolation has ever been reported from cerebrospinal fluid or in india. the first case of r. paucula meningitis and septic ... | 2013 | 24064653 |
| identification of two combined genes responsible for dechlorination of 3,5,6-trichloro-2-pyridinol (tcp) in cupriavidus pauculus p2. | dehalogenation is an important mechanism for degrading and detoxifying halogenated aromatics in microbes. however, the biochemical and molecular mechanisms of dehalogenation of 3,5,6-trichloro-2-pyridinol (tcp) are still unknown. in this study, a novel 6012 bp gene cluster was cloned from tcp-degrading strain p2, which was responsible for the dehalogenation of tcp. the cluster included a monooxygenase gene (tcpa1), a flavin reductase gene (tcpb1), tcpr1, orf1 and orf2. tcpa1 and tcpb1 were indis ... | 2013 | 23850940 |
| a case of newborn with community acquired pneumonia caused by cupriavidus pauculus. | cupriavidus pauculus is a gram-negative, aerobic, non-spore forming, non-fermentative motile bacillus. the bacillus can be isolated from water, bottled mineral water, and water from ultrafiltration systems in hospital setting. c. pauculus rarely causes human infections, however it may be an infectious agent especially in immunocompromised individuals. in this report, we present the first case of community acquired pneumonia caused by c. pauculus in a previously healthy newborn who was hospitaliz ... | 2012 | 22779937 |
| characterization of a newly isolated highly effective 3,5,6-trichloro-2-pyridinol degrading strain cupriavidus pauculus p2. | a bacterial strain p2 capable of degrading 3,5,6-trichloro-2-pyridinol (tcp) was isolated and characterized. phylogenetic analysis based on 16s rrna gene sequence indicated that it belonged to the genus of cupriavidus, because it showed the highest sequence similarity to cupriavidus pauculus lmg 3413(t) (99.7 %) and dna-dna relatedness value between strain p2 and c. pauculus lmg 3413(t) was 76.8 %. in combination with morphological, physiological and biochemical characters, strain p2 was identif ... | 2012 | 22645014 |