| purification and characterization of lipase from a raw-milk yeast (trichosporon asteroides). | a lipase-producing yeast strain was isolated from raw milk. it was identified as trichosporon asteroides strain lp005. the lipase from this yeast was purified 8-fold to homogeneity for further characterization. the purification process for this lipase included (nh4)2so4 precipitation at 70% saturation and gel filtration on sephadex g-200. the molecular mass of the lipase was 37 kda as determined by sds/page. the optimum ph and temperature for activity were ph 5.0 and 60 degrees c. the lipase was ... | 1997 | 9357107 |
| biodiversity of killer activity in yeasts isolated from the brazilian rain forest. | the occurrence of killer activity against a panel composed of 22 industrially and (or) medically important yeasts was investigated in 438 yeast and yeast-like cultures belonging to 96 species, isolated from different environments of the brazilian rain forest. altogether, 26% of ascomycetes, 56% of basidiomycetes, and 42% of yeast-like cultures exhibited killer activity against at least one of the panel yeasts. more than 15 species never reported before as toxin producers were found, with pseudoz ... | 2000 | 10932353 |
| nosocomial fungemia due to trichosporon asteroides: firstly described bloodstream infection. | trichosporon spp. are oppurtunistic yeasts that cause deep-seated, mucosa-associated, and superficial infections in immunocompromised patients. it is well known that trichosporon asteroides is mainly responsible of superficial infections and does not cause systemic infections in humans so far. in this study, we present the first case of disseminated infection due to trichosporon asteroides in an intensive care patient. yeast colonies were isolated from the specimens of blood, urine, aspiration f ... | 2002 | 12088626 |
| characterization of trichosporon species isolated from clinical specimens in kuwait. | invasive trichosporonosis is an emerging infection of severely immunocompromised patients. it is principally caused by trichosporon asahii, although some other species of the genus have also been implicated in the aetiology. in this work, 29 clinical isolates of trichosporon species recovered from 29 different patients over a 6-year period were studied for phenotypic and genotypic characteristics. two morphotypes were recognized on the basis of colony characteristics. the colonies of 25 isolates ... | 2005 | 15947428 |
| molecular phylogenetics of the genus trichosporon inferred from mitochondrial cytochrome b gene sequences. | mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus trichosporon were partially sequenced to determine their molecular phylogenetic relationships. almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. analysis of a 396-bp coding sequence from each strain of trichosporon under investigation showed a total of 141 (35.6%) variable nucleotide sites. a phylogenetic tree based on the cyt b gene sequences rev ... | 2005 | 16207980 |
| occurrence of pathogenic fungi in soil of burrows of rats and of other sites in bamboo plantations in india and nepal. | this study examined 215 samples of soil from burrows of rats, other sites in bamboo plantations in different parts of india and nepal by dilution plating and mouse passage technique for occurrence of penicillium marneffei and other pathogenic fungi. none of the samples including 25 collected from the burrows of a bamboo rat (cannomys badius) known to be a carrier of p. marneffei, was positive for the fungus. among the pathogenic fungi recovered were four isolates of pseudallescheria boydii (incl ... | 2007 | 17944715 |
| bloodstream infections due to trichosporon spp.: species distribution, trichosporon asahii genotypes determined on the basis of ribosomal dna intergenic spacer 1 sequencing, and antifungal susceptibility testing. | the reevaluation of the genus trichosporon has led to the replacement of the old taxon trichosporon beigelii by six new species. sequencing of the ribosomal dna (rdna) intergenic spacer 1 (igs1) is currently mandatory for accurate trichosporon identification, but it is not usually performed in routine laboratories. here we describe trichosporon species distribution and prevalence of trichosporon asahii genotypes based on rdna igs1 sequencing as well as antifungal susceptibility profiles of 22 is ... | 2009 | 19225102 |
| antifungal susceptibility profile of clinical trichosporon asahii and trichosporon asteroides isolates identified by molecular methods. | | 2009 | 19567360 |
| characterization of the gastrointestinal yeast microbiota of cockatiels (nymphicus hollandicus): a potential hazard to human health. | cockatiels are the world's second most popular psittacine pet bird, but no data characterizing their gastrointestinal microbiota have been found. thus, the aim of this work was to characterize the yeast gastrointestinal microbiota of cockatiels and to evaluate the relevance of cockatiels as carriers of potentially pathogenic yeasts. a total of 60 cockatiels, from 15 different premises, were assessed. a thorough clinical examination was performed with each bird, and samples were collected from or ... | 2010 | 20150318 |
| escmid and ecmm joint clinical guidelines for the diagnosis and management of rare invasive yeast infections. | the mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than candida being no exception. this is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifung ... | 2014 | 24102785 |
| differentiation of the emerging human pathogens trichosporon asahii and trichosporon asteroides from other pathogenic yeasts and moulds by using species-specific monoclonal antibodies. | the fungal genus trichosporon contains emerging opportunistic pathogens of humans, and is the third most commonly isolated non-candidal yeast from humans. trichosporon asahii and t. asteroides are the most important species causing disseminated disease in immunocompromised patients, while inhalation of t. asahii spores is the most important cause of summer-type hypersensitivity pneumonitis in healthy individuals. trichosporonosis is misdiagnosed as candidiasis or cryptococcosis due to a lack of ... | 2014 | 24392156 |
| antifungal susceptibility of emerging opportunistic yeasts and yeast-like fungi from rhea americana. | opportunistic yeasts and yeast-like fungi have been recognized as important pathogens in high-risk patients. this study aimed to evaluate the presence of these microorganisms in the microbiota of captive rheas and to investigate the antifungal susceptibility of the isolated strains. isolates representing magnusiomyces capitatus (geotrichum capitatum, n = 11), trichosporon mucoides (n = 11), trichosporon asteroides (n = 5), rhodotorula mucilaginosa (n = 4), trichosporon asahii (n = 3), trichospor ... | 2013 | 23899001 |
| sequencer-based capillary gel electrophoresis (scge) targeting the rdna internal transcribed spacer (its) regions for accurate identification of clinically important yeast species. | accurate species identification of candida, cryptococcus, trichosporon and other yeast pathogens is important for clinical management. in the present study, we developed and evaluated a yeast species identification scheme by determining the rdna internal transcribed spacer (its) region length types (lts) using a sequencer-based capillary gel electrophoresis (scge) approach. a total of 156 yeast isolates encompassing 32 species were first used to establish a reference scge its lt database. evalua ... | 2016 | 27105313 |
| molecular characterisation and antifungal susceptibility of clinical trichosporon isolates in india. | in asian countries, trichosporon infection is a well-known disease in japan. in india, the infection is increasingly recognised. the study was conducted to characterise the clinical trichosporon isolates from india by phenotypic and molecular techniques. a total of 31 trichosporon clinical isolates, recovered from patients of 14 hospitals across india were sequenced (its and igs1 regions of rdna). in vitro drug susceptibility testing of the isolates was performed against amphotericin-b, fluconaz ... | 2016 | 27144725 |