| disseminated infection with trichosporon asahii. | trichosporon fungaemia and disseminated, purpuric, papular skin lesions developed on the head, trunk and extremities of a 5-year-old female with acute lymphocytic leukaemia. histopathologically, the skin lesions demonstrated dermal budding yeasts. she died despite treatment with antifungal drugs. the isolate from the blood was further identified morphologically and physiologically as trichosporon asahii, based on the revision of the genus trichosporon by guého et al. (1992). according to the new ... | 1996 | 8909029 |
| effects of granulocyte and granulocyte-macrophage colony-stimulating factors in a neutropenic murine model of trichosporonosis. | we produced disseminated trichosporonosis in a neutropenic murine model with trichosporon asahii, which was identified by dna relatedness analysis. we then assessed the efficacy of granulocyte colony-stimulating factor (g-csf) (30 to 100 microg/kg of body weight per day) and granulocyte-macrophage colony-stimulating factor (gm-csf) (0.8 to 2 microg/kg x day). the administration of g-csf either before or after infection improved the survival rate from less than 25% up to 100% (p < 0.05). the effe ... | 1997 | 9234807 |
| experimental disseminated trichosporonosis in mice: tissue distribution and therapy with antifungal agents. | mice treated with cyclophosphamide were infected intravenously with titrated doses of trichosporon asahii timm3140 or timm3144. mortality and survival time correlated with inoculum size and fungal growth was found in the lung, heart, liver, spleen, kidney and brain. fluconazole and amphotericin b prolonged survival time and reduced the cfu in the kidney, with fluconazole being superior to amphotericin b. the serum level of (1-->3)-beta-d-glucan was reduced by antifungal therapy and correlated we ... | 1997 | 9467108 |
| seasonal chronic cough with sputum eosinophilia caused by trichosporon cutaneum (trichosporon asahii). | the case of a 46-year-old man with a chronic cough with sputum eosinophilia (atopic cough) caused by trichosporon cutaneum serotype ii (trichosporon asahii) is reported. the diagnosis was made with the inhalation challenge test with t. asahii antigen. he was admitted for the diagnosis and treatment of a severe nonproductive cough in the summer season. although his sputum contained 13% eosinophils of nucleated cells, he did not have bronchial hyperresponsiveness to methacholine or a heightened br ... | 1998 | 9652310 |
| identification of trichosporon asahii by pcr based on sequences of the internal transcribed spacer regions. | trichosporon asahii is a major causative agent of deep-seated trichosporonosis, which has a high mortality rate. to detect t. asahii, we have developed specific oligonucleotide primers based on the internal transcribed spacer regions of this organism's genome. amplification products were selectively obtained from only t. asahii dna; the dnas of other trichosporon species, as well as those of medically relevant yeasts such as candida albicans, cryptococcus neoformans, and malassezia furfur, were ... | 1998 | 9705427 |
| pcr detection of dna specific for trichosporon species in serum of patients with disseminated trichosporonosis. | deep-seated trichosporonosis is a lethal opportunistic infection that disseminates rapidly and widely in immunocompromised patients, and early diagnosis is crucial for the treatment of this infection. we developed a novel nested-pcr assay that detects dna specific for clinically important strains of trichosporon in serum samples from patients with disseminated trichosporonosis. in this assay, two sets of oligonucleotide primers were derived from the sequence of 26s rrna genes of trichosporon asa ... | 1999 | 9986834 |
| disseminated trichosporonosis with trichosporon asahii. | trichosporon asahii fungemia was associated with multiple, purpuric, papular lesions on the abdomen and extremities in a 53-year-old man with acute myeloblastic leukemia. histologically, budding yeasts were demonstrated in the dermis. the yeast-form fungus was identified as t. asahii. | 1999 | 10523742 |
| effects of macrophage colony-stimulating factor (m-csf) on anti-fungal activity of mononuclear phagocytes against trichosporon asahii. | trichosporon asahii is an emerging opportunistic pathogen in immunocompromised patients. little is known about the mechanisms of host defence against t. asahii. we investigated the fungicidal activity of human peripheral blood monocytes and murine peritoneal macrophages against t. asahii isolates, and the effects of m-csf on the anti-fungal activity of mononuclear phagocytes. we also established a neutropenic mouse model of disseminated trichosporonosis with t. asahii. m-csf enhanced the phagocy ... | 2000 | 10632665 |
| isolation of trichosporon asahii from environmental materials. | trichosporon asahii is the most clinically important pathogenic yeast in the genus trichosporon, as this species causes both deep-seated infection and summer-type hypersensitivity pneumonitis. we isolated 29 t. asahii colonies from environmental samples using the polymerase chain reaction (pcr) and dichloran rose bengal chloramphenicol (drbc) medium. our results suggest that t. asahii is common in nature. | 2000 | 10746224 |
| trichosporon cutaneum (trichosporon asahii) infection mimicking hand eczema in a patient with leukemia. | trichosporon cutaneum is a yeast-like fungus that causes white piedra and onychomycosis. recently, it has also been recognized as an opportunistic pathogen in immunocompromised hosts. we describe a 64-year-old woman who developed a superficial trichosporon infection mimicking hand eczema during chemotherapy for her chronic myelocytic leukemia. to our knowledge, no cases of superficial infection like this one have previously been reported. this case suggests that careful examination is required i ... | 2000 | 10767708 |
| [pathogenesis and treatment of hypersensitivity pneumonitis]. | hypersensitivity pneumonitis (hp) is a granulomatous interstitial lung disease resulting from an immunologic reaction to the repeated inhalation of organic dusts and active chemicals. there are 50 or more groups of hp, but the prevalence varies from country to country, and even within a country, depending on a variety of occupational or environmental inhalants. in western coutries farmer's lung, bird fancier's disease, humidifier lung, and air-conditioner disease are common, but in japan summer- ... | 2000 | 10938513 |
| experimental model of progressive disseminated trichosporonosis in mice with latent trichosporonemia. | trichosporon asahii and trichosporon mucoides are the most common strains of fungi that cause disseminated trichosporonosis, a severe opportunistic infection in immunocompromised hosts. we have previously established a nested pcr assay using serum samples for detection of both strains. here we describe a new experimental animal model for investigating the underlying mechanisms of disseminated trichosporonosis. t. asahii (omu239, a clinical isolate from a patient with acute myelogenous leukemia) ... | 2000 | 10970368 |
| differential host susceptibility to pulmonary infections with bacteria and fungi in mice deficient in myeloperoxidase. | myeloperoxidase (mpo), which is located within neutrophils capable of producing hypochlorous acid, is active in vitro against bacteria and fungi. however, mpo-deficient persons are usually healthy. to define the in vivo contribution of mpo to early host defense against pulmonary infections, mpo-deficient and control mice were intranasally infected with various fungi and bacteria, and the number of residual microorganisms in lungs was compared 48 h later. mpo-deficient mice showed severely reduce ... | 2000 | 10979934 |
| blood lysate staining, a new microscopic method for diagnosis of fungemia using peripheral blood. | we developed a microscopy method for the detection of fungal cells in peripheral blood, termed blood lysate staining, using an approximately 5x5 mm dotted blood lysate. this method was able to detect the emerging fungal pathogen trichosporon asahii in murine models of systemic fungal infection and fungemia in patients quickly and at minimal cost. pathogenic yeasts were successfully detected in 6 of 8 blood samples which were taken from feverish immunocompromised patients who were clinically susp ... | 2000 | 11021393 |
| a nested pcr assay to detect dna in sera for the diagnosis of deep-seated trichosporonosis. | deep-seated trichosporonosis caused by trichosporon asahii has a high mortality rate and a very poor prognosis. new species-specific oligonucleotide primers for t. asahii were developed from a sequence analysis of rrna genes that included the internal transcribed spacer regions. a nested pcr assay with specific primers was used to examine 11 serum samples from 7 patients, who were diagnosed with deep-seated trichosporonosis histologically at autopsy. in addition, trichosporon cell wall polysacch ... | 2001 | 11293480 |
| [clinical usefulness of antigen-captured elisa method using mouse anti-trichosporon asahii monoclonal antibody d-8 for diagnosis of summer-type hypersensitivity pneumonitis]. | in the present study, we assessed the clinical usefulness of the antigen-captured elisa method using mouse anti-trichosporon asahii monoclonal antibody d-8 in 193 patients with summer-type hypersensitivity pneumonitis (shp) and in 127 healthy volunteers. when the cut-off value was determined as 0.15, the diagnosis of shp was accurately made by this method in 168 of 193 shp patients. the sensitivity and specificity of the diagnosis of shp determined by this method were 87.0% and 96.1%, respective ... | 2001 | 11296391 |
| trichosporon species infection in bone marrow transplanted patients. | trichosporon species are emerging as opportunistic agents that cause systemic diseases in immunocompromised patients. patients undergoing bone marrow transplant are submitted to intense and prolonged periods of neutropenia and consequently to several risk factors to fungal infections as the use of broad spectrum antibiotics and invasive devices. two cases of fungal infections caused by trichosporon asahii var. asahii and t. inkin in patients with bone marrow transplant are described t. asahii va ... | 2001 | 11337182 |
| transmission of trichosporon asahii oesophagitis by a contaminated endoscope. | two cases of oesophageal trichosporonosis due to a suspected nosocomial infection are reported. both the patients were immunocompetent and had undergone an endoscopic examination on the same day. six strains of trichosporon were isolated: three strains from the oesophageal biopsy of the first patient, one strain from the endoscopic forceps, one from the air in the endoscopy room, and one from the oesophageal biopsy of the second patient. the nosocomial nature of the infection and the role of the ... | 2001 | 11398636 |
| genetic diversity and biochemical characteristics of trichosporon asahii isolated from clinical specimens, houses of patients with summer-type-hypersensitivity pneumonitis, and environmental materials. | trichosporon asahii, which is distributed in the environment, is the major causative agent of the opportunistic infection trichosporonosis, and it also causes summer-type hypersensitivity pneumonitis (shp). random amplification of polymorphic dna analysis was used to determine the intraspecies diversity of 39 t. asahii isolates from clinical specimens, shp patients' houses, and environmental materials. the three primers used revealed 46 polymorphic bands. a phenogram was generated by the unweigh ... | 2001 | 11427546 |
| trichosporon asahii, a non-candida yeast that caused fatal septic shock in a patient without cancer or neutropenia. | trichosporon asahii (formerly trichosporon beigelii) is an emerging fungal pathogen seen particularly in immunologically compromised patients. there are now approximately 100 reported cases of hematogenously disseminated infections with this life-threatening yeast, and no effective antifungal therapy is available. the present case is unusual because the patient did not have neutropenia or evidence of a malignancy. | 2001 | 11477533 |
| structural studies of a cell wall polysaccharide of trichosporon asahii containing antigen ii. | the structure of a cell-wall polysaccharide containing antigen ii from trichosporon asahii was investigated. a purified glucuronoxylomannan (gxm) antigen was found to contain o-acetyl groups that contribute to the serological reactivity. the structure of gxm was analyzed by partial acid hydrolysis, methylation analysis, controlled smith degradation, nmr studies, and fluorophore-assisted carbohydrate electrophoresis. gxm has an alpha-(1-->3)-d-mannan backbone with a beta-d-glucopyranosyluronic ac ... | 2001 | 11589701 |
| multidrug-resistant trichosporon asahii infection of nongranulocytopenic patients in three intensive care units. | trichosporon asahii (trichosporon beigelii) infections are rare but have been associated with a wide spectrum of clinical manifestations, ranging from superficial involvement in immunocompetent individuals to severe systemic disease in immunocompromised patients. we report on the recent recovery of t. asahii isolates with reduced susceptibility in vitro to amphotericin b (amb), flucytosine, and azoles from six nongranulocytopenic patients who exhibited risk factors and who developed either super ... | 2001 | 11724855 |
| [26s rdna d1/d2 domain sequence analysis of the pathogenic strain from the first case of disseminated trichosporonosis in china]. | to determine the large-subunit (26s) rdna d1/d2 domain sequence of as 2.217 4, the pathogenic strain from the first case of disseminated trichosporosis in china, and study the feasibility of applying dna sequencing in rapid identification of pathogenic yeast. | 2001 | 11798921 |
| trichosporon asahii: an unusual cause of invasive infection in neonates. | trichosporon asahii causes white piedra, an infection of hair shafts and onychomycosis in immunocompetent patients, as well as various localized or disseminated invasive infections in immunodeficient hosts. we describe a 26-week gestation 890-g vaginally delivered female neonate who had severe respiratory distress syndrome and on the sixth day of life developed klebsiella pneumoniae sepsis. at the same time two blood cultures were positive for t. asahii. the neonate was also colonized with t. as ... | 2002 | 11840088 |
| generalized lymphadenopathy caused by trichosporon asahii in a patient with job's syndrome. | of the six species of trichosporon known to cause human infections, t. asahii is the main agent of invasive trichosporonosis. we describe an unusual case of generalized lymphadenopathy due to t, asahii in a 10-year-old boy with job's syndrome (markedly elevated ige with eosinophilia). the diagnosis was based on the presence of blastic conidia and hyphal elements breaking into arthroconidia in biopsied tissue of the cervical lymph node and isolation of the causal agent t, asahii in pure culture. ... | 2002 | 11862981 |
| trichosporon asahii-induced asthma in a family with japanese summer-type hypersensitivity pneumonitis. | trichosporon species frequently induce summer-type hypersensitivity pneumonitis (shp), which is the most prevalent type of hypersensitivity pneumonitis (hp) in japan, but have not been reported to induce asthma. | 2002 | 11926630 |
| antifungal pharmacodynamic characteristics of amphotericin b against trichosporon asahii, using time-kill methodology. | we determined the mic of amphotericin b against 45 trichosporon asahii isolates from various clinical and environmental sources, and used in vitro time-kill methods to characterize the relationship between amphotericin b concentrations and mic for four representative t. asahii isolates. amphotericin b had concentration-dependent antifungal activity. mics ranged from 0.5 to 16 microg/ml, and most t. asahii isolates (76%, 34/45) were inhibited at safely achievable amphotericin b serum concentratio ... | 2002 | 11939583 |
| increased release of glucuronoxylomannan antigen and induced phenotypic changes in trichosporon asahii by repeated passage in mice. | clinically important fungi such as candida albicans and cryptococcus neoformans are known to undergo phenotypic changes after repeated subculture or passages in vivo. however, there are no reports describing this phenomenon in trichosporon species. this study investigated whether in-vivo passages of environmental isolates of trichosporon asahii in mice changes their phenotype; three environmental isolates and 14 clinical isolates (from deep-seated infections) were used. the shape of the colony a ... | 2002 | 11990495 |
| chronic disseminated trichosporon asahii infection in a leukemic child. | we report a case of chronic disseminated trichosporon asahii infection in a leukemic child. administration of amphotericin b lipid complex resulted in rapid control and improvement of the initial infection but failed to prevent the development of chronic disseminated disease. cure was achieved after treatment was changed to a 20-month course of itraconazole. this case report demonstrates that trichosporon species can cause chronic disseminated disease with a pattern similar to that of disease ca ... | 2002 | 12087540 |
| comparison of nccls microdilution method and etest in antifungal susceptibility testing of clinical trichosporon asahii isolates. | we investigated the in vitro activity of amphotericin b, fluconazole, and itraconazole against clinical trichosporon asahii isolates (n = 43) by nccls m27a reference microdilution method and explored the correlation between etest and nccls reference method. microdilution mic ranges following 48 h of incubation were 1-8, 0.25-16, and 0.06-4 microg/ml for amphotericin b, fluconazole, and itraconazole, respectively. the corresponding etest mic ranges were determined as 0.125- > 8, 0.25- > 64, and 0 ... | 2002 | 12088616 |
| identification of the first isolates of trichosporon asahii var asahii from disseminated trichosporonosis in china. | infection with trichosporon asahii is a major cause of deep-seated and disseminated trichosporonosis, which is associated with a high mortality rate. disseminated trichosporonosis in individuals with no underlying disease has not been reported. in this study, we report the identification of the first isolate of trichosporon asahii var. asahii in china. two isolates were obtained from the liver and skin of a patient with disseminated trichosporonosis who displayed no evidence of underlying diseas ... | 2002 | 12376026 |
| ta-19, a novel protein antigen of trichosporon asahii, in summer-type hypersensitivity pneumonitis. | the most common form of hypersensitivity pneumonitis in japan is summer-type hypersensitivity pneumonitis (shp), which is caused by the inhalation of trichosporon asahii or trichosporon mucoides. to seek protein antigens relevant to the immunopathogenesis of shp, we constructed a cdna expression library of t. asahii, a major causative yeast species of shp. using the immunoscreening method, we identified and cloned a novel gene encoding a 19-kd protein, named ta-19, which proved to be specificall ... | 2003 | 12493647 |
| use of voriconazole to successfully treat disseminated trichosporon asahii infection in a patient with acute myeloid leukaemia. | trichosporon spp. is an emerging fungal pathogen in immunocompromised hosts, and disseminated infection is often fatal in neutropenic patients. reported here is a case of disseminated infection in a neutropenic patient with acute leukaemia. after failure of amphotericin b and fluconazole therapy, the course of infection dramatically improved with voriconazole treatment. a literature search revealed 69 additional cases of disseminated trichosporon spp. infections in neutropenic patients, and thes ... | 2002 | 12525928 |
| candida tropicalis in a neonatal intensive care unit: epidemiologic and molecular analysis of an outbreak of infection with an uncommon neonatal pathogen. | from june to july 1998, two episodes of candida tropicalis fungemia occurred in the aristotle university neonatal intensive care unit (icu). to investigate this uncommon event, a prospective study of fungal colonization and infection was conducted. from december 1998 to december 1999, surveillance cultures of the oral cavities and perinea of the 593 of the 781 neonates admitted to the neonatal icu who were expected to stay for >7 days were performed. potential environmental reservoirs and possib ... | 2003 | 12574275 |
| multidrug-resistant trichosporon asahii isolates are susceptible to voriconazole. | | 2003 | 12574315 |
| [intraspecies diversity of trichosporon asahii as the causative agent of opportunistic fungal infection and summer-type hypersensitivity pneumonitis]. | trichosporon asahii is the major causative agent of the opportunistic infection trichosporonosis, and also causes summer-type hypersensitivity pneumonitis (shp). random amplification of polymorphic dna analysis was used to determine the intraspecies diversity of t. asahii isolates from clinical specimens,the houses of shp patients, and environmental material. clinical isolates formed a cluster, characterized by a 90% matching coefficient, but they did not cluster with strains isolated from shp p ... | 2003 | 12590253 |
| identification of yeast species from orange fruit and juice by rflp and sequence analysis of the 5.8s rrna gene and the two internal transcribed spacers. | yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. the first was analysis of the restriction pattern generated from the polymerase chain reaction (pcr)-amplified 5.8s rrna gene and the two internal transcribed spacers (its) using specific primers. the second technique was sequence analysis of the its regions using the same two primers. nine different restriction profiles were obtained from the size of the pcr produ ... | 2003 | 12702240 |
| yeasts associated with manteca. | manteca is a traditional milk product of southern italy produced from whey deriving from caciocavallo podolico cheese-making. this study was undertaken to obtain more information about the microbiological properties of this product and particularly about the presence, metabolic activities, and technological significance of the different yeast species naturally occurring in manteca. high numbers of yeasts were counted after 7 days ripening (10(4)-10(5) cfu g(-1)) and then decreased to 10(2) at th ... | 2003 | 12702448 |
| specific oligonucleotide primers for identification of hortaea werneckii, a causative agent of tinea nigra. | hortaea werneckii, a black yeast-like hyphomycete that is widely distributed in tropical and subtropical environments, can cause superficial mycotic infection in humans. this fungus was recently isolated from superficial infectious lesions of a guinea pig in japan. an oligonucleotide primer set specific for hortaea werneckii was designed on the basis of the internal transcribed spacer regions of the ribosomal dna (rdna). polymerase chain reaction (pcr) with this primer set yielded a 306 bp pcr a ... | 2003 | 12812723 |
| [nosocomial urinary infection due to trichosporon asahii. first two cases in chile]. | we present two cases of nosocomial urinary tract infection due to trichosporon asahii in intensive care unit patients with bladder catheter from two hospitals in santiago, chile. both patients had an several catheters and bacterial infections that required the use of antibiotic therapy. one strain showed in vitro resistance to amphotericin b. both strains were susceptible to fluconazole, but presented mic with dose-dependent susceptibility to ketoconazole and itraconazole. this is the first repo ... | 2003 | 12825977 |
| [first chilean report of nosocomial urinary tract infection due to trichosporon asahii, in 2 patients]. | | 2003 | 12870243 |
| [pathogenesis of trichosporon asahii and strategies for infectious control of disseminated trichosporonosis]. | deep-seated trichosporonosis is a lethal opportunistic infection in immunocompromised patients. trichosporon asahii and t. mucoides are the most common strains of fungi that cause disseminated trichosporonosis. thirteen patients were diagnosed with disseminated trichosporonosis over a 20 year period in oita medical university hospital. the majority of them had underlying hematologic malignancies, for which they received cytotoxic chemotherapy resulting in neutropenia. for the rapid diagnosis of ... | 2003 | 12913806 |
| disseminated trichosporon asahii infection in a preterm. | trichosporon asahii (t. asahii) is an uncommon cause of yeast infection in preterms. we present a 27-week gestational age female with clinical evidence of sepsis, such as patchy infiltrations on chest roentgenogram, and yeast growing in urine and blood cultures. conventional amphotericin b was empirically added in a dose of 0.5 mg/kg, q8h to standard protocol of the neonatal intensive care unit. dose of the drug was induced to 1 mg/kg because the patient had not improved when the organism was id ... | 2003 | 13680511 |
| disseminated trichosporonosis in china. | a 20-year-old female patient presented with erythematous plaques on the nose which were progressively spreading to the trunk and the extremities, sometimes with erosions and scars. the patient was misdiagnosed as having seborrhoeic dermatitis and subacute cutaneous lupus erythematosus. the histopathological biopsy revealed mycotic infectious granuloma. samples taken from skin lesions and other locations grew trichosporon asahii in cultures. the identification was confirmed by molecular biologica ... | 2003 | 14641628 |
| suppression of rhizoctonia solani diseases of sugar beet by antagonistic and plant growth-promoting yeasts. | isolates of candida valida, rhodotorula glutinis and trichosporon asahii from the rhizosphere of sugar beet in egypt were examined for their ability to colonize roots, to promote plant growth and to protect sugar beet from rhizoctonia solani ag-2-2 diseases, under glasshouse conditions. | 2004 | 14678160 |
| phenotypic switching and beta-n-acetylhexosaminidase activity of the pathogenic yeast trichosporon asahii. | the pathogenic yeast trichosporon asahii is the major causative agent of deep-seated trichosporonosis in immunocompromised patients. although infection by this microorganism is becoming increasingly frequent, information related to its pathogenicity and virulence factors is still limited. therefore, we investigated phenotypic switching in colony morphology, and the production of extracellular enzymes as a virulence factor. sixty-one clinical isolates of t. asahii produced four different morpholo ... | 2004 | 15107533 |
| trichosporon asahii infection treated with caspofungin combined with liposomal amphotericin b. | | 2004 | 15231763 |
| trichosporon asahii infection of a dialysis ptfe arteriovenous graft. | trichosporon species are the causative agents of superficial skin infections, such as white piedra. immunocompromised hosts, particularly those with underlying hematological malignancy, are at risk of developing invasive infection, which usually progresses to disseminated life-threatening disease. peritonitis caused by trichosporon has been described in end-stage renal disease patients treated with continuous ambulatory peritoneal dialysis. here, we report on a trichosporon infection of an arter ... | 2004 | 15267017 |
| comparative evaluation of standard dilution method and commercial kit for frozen plate antifungal susceptibility testing of yeasts using 200 clinical isolates. | a comparative evaluation of standard microdilution methods and a commercial kit for frozen plate antifungal susceptibility testing of yeasts was performed using amphotericin b, flucytosine, fluconazole, miconazole, and itraconazole on 200 yeast isolates. the isolates included 100 strains of candida albicans, eight of c. tropicalis, twelve of c. parapsilosis, eight of c. glabrata, five of cryptococcus neoformans, thirteen of trichosporon asahii, and 54 other strains of seven other species of asco ... | 2004 | 15502407 |
| marked renal damage in a child with hydronephrosis infected by trichosporon asahii. | we describe a case of urinary infection caused by trichosporon asahii, which drastically aggravated renal function in a left hydronephrotic kidney. previous implantation of a nephrostomy tube and a ureteral j-stent and administration of broad-spectrum antibiotics seemed to have predisposed the non-granulocytopenic child to the urinary fungal infection. | 2004 | 15625616 |
| bioconversion of lutein using a microbial mixture--maximizing the production of tobacco aroma compounds by manipulation of culture medium. | the generation of aroma compounds by carotenoid cleavage in the 9-10 position was studied, due to the importance of these compounds in the flavor and fragrance industry. the bioconversion of the carotenoid lutein to c(13) norisoprenoids utilizing a microbial mixture composed of trichosporon asahii and paenibacillus amylolyticus was carried out by a fermentation process. applying an experimental design methodology, the effects of nutritional factors on the production of aroma compounds present in ... | 2005 | 15660215 |
| efficacy of micafungin in combination with other drugs in a murine model of disseminated trichosporonosis. | using a murine model of disseminated infection caused by trichosporon asahii, we have evaluated the efficacies of amphotericin b (amb; 1 mg/kg of body weight/day), fluconazole (flc; 20 mg/kg/twice a day), and micafungin (mfg; 5 mg/kg/twice a day). we tested these drugs alone and in combination (mfg with amb and mfg with flc). mfg with amb showed a synergistic effect and demonstrated a higher degree of efficacy in prolonging survival and reducing the kidney fungal burden than either agent alone. ... | 2005 | 15673724 |
| isolation of trichosporon in a hematology ward. | during mycologic monitoring of the air in a hematology ward, we found massive air contamination caused by trichosporon asahii, both in the room where neutropenic patients were staying and the corridor immediately outside the room. this fungal species had never been isolated in previous samplings. the urine culture taken from one of the patients in this room, whose urinary catheter had been removed immediately prior to air sampling, resulted positive for t. asahii. both macroscopic and microscopi ... | 2005 | 15679666 |
| occurrence and diversity of yeasts involved in fermentation of west african cocoa beans. | samples of cocoa beans were taken on two separate occasions during heap and tray fermentations in ghana, west africa. in total 496 yeast isolates were identified by conventional microbiological analyses and by amplification of their its1-5.8s rdna-its2 regions. for important species the identifications were confirmed by sequencing of the d1/d2 domain of the 5' end of the large subunit (26s) rdna. assimilations of organic acids and other carbon compounds were conducted. for dominant yeasts intras ... | 2005 | 15691749 |
| the yeast flora of some decaying mushrooms on trunks of living trees. | several ascomycetous and basidiomycetous yeasts were isolated from rotten mushrooms on the trunks of beech and tamarisk trees. one strain, identified as the novel species cryptococcus allantoinivorans, assimilated allantoin as the sole carbon source. phylogenetically it belongs to the c. laurentii complex, papiliotrema bandonii being the closest relative. some ascomycetous strains could not be distinguished from pichia guillermondii, but deviated considerably in rdna sequences. in addition to th ... | 2004 | 15702547 |
| yeast populations associated with ghanaian cocoa fermentations analysed using denaturing gradient gel electrophoresis (dgge). | the yeast populations associated with the fermentation of ghanaian cocoa were investigated using denaturing gradient gel electrophoresis (dgge). samples were collected at 12-24 h intervals from heap and tray fermentations, at three different fermentation sites and different periods during the season. eukaryotic universal primers were used to amplify a fragment of the 26s rrna gene. the dgge profiles were relatively complex, underlining that the fermentation of cocoa is a complex microbial proces ... | 2005 | 15704234 |
| in vitro interaction of micafungin with conventional and new antifungals against clinical isolates of trichosporon, sporobolomyces and rhodotorula. | the infections caused by basidiomycetous yeasts are often difficult to resolve. combined therapy might be useful in those severe cases where a monotherapy was ineffective. the aim of this study was to evaluate the in vitro activity of combinations of micafungin with amphotericin b or fluconazole, itraconazole, voriconazole and ravuconazole against isolates of trichosporon, rhodotorula and sporobolomyces. | 2005 | 15845779 |
| fungemia due to trichosporon asahii in a neutropenic child refractory to amphotericin b: clearance with voriconazole. | disseminated trichosporon infection in neutropenic patients carries a poor prognosis. clinical evidence on the use of voriconazole for this infection is limited. the authors report a case of trichosporon asahii fungemia refractory to liposomal amphotericin b treatment in a boy with acute lymphoblastic leukemia, which resolved after the addition of voriconazole. both voriconazole and amphotericin b exhibited low minimal inhibitory concentrations and minimal fungicidal concentrations, and their co ... | 2005 | 15891566 |
| characterization of trichosporon species isolated from clinical specimens in kuwait. | invasive trichosporonosis is an emerging infection of severely immunocompromised patients. it is principally caused by trichosporon asahii, although some other species of the genus have also been implicated in the aetiology. in this work, 29 clinical isolates of trichosporon species recovered from 29 different patients over a 6-year period were studied for phenotypic and genotypic characteristics. two morphotypes were recognized on the basis of colony characteristics. the colonies of 25 isolates ... | 2005 | 15947428 |
| susceptibility patterns and molecular identification of trichosporon species. | the physiological patterns, the sequence polymorphisms of the internal transcriber spacer (its), and intergenic spacer regions (igs) of the rrna genes and the antifungal susceptibility profile were evaluated for their ability to identify trichosporon spp. and their specificity for the identification of 49 clinical isolates of trichosporon spp. morphological and biochemical methodologies were unable to differentiate among the trichosporon species. its sequencing was also unable to differentiate s ... | 2005 | 16189076 |
| molecular phylogenetics of the genus trichosporon inferred from mitochondrial cytochrome b gene sequences. | mitochondrial cytochrome b (cyt b) genes of 42 strains representing 23 species of the genus trichosporon were partially sequenced to determine their molecular phylogenetic relationships. almost half of the 22 strains investigated (from 11 different species) contained introns in their sequences. analysis of a 396-bp coding sequence from each strain of trichosporon under investigation showed a total of 141 (35.6%) variable nucleotide sites. a phylogenetic tree based on the cyt b gene sequences rev ... | 2005 | 16207980 |
| fatal trichosporon asahii septicemia in a guatemalan farmer with acute lymphoblastic leukemia. | | 2005 | 16217998 |
| relationship between mic and minimum sterol 14{alpha}-demethylation-inhibitory concentration as a factor in evaluating activities of azoles against various fungal species. | the minimum growth-inhibitory concentrations (mics) of azole antifungals were compared to their minimum sterol 14alpha-demethylation-inhibitory concentrations (mdics) for clinical fungal isolates. the ascomycetous candida yeasts tested were clearly divided into two groups: group i, consisting of c. albicans, c. tropicalis, and c. lusitaniae, had mics that were much higher than the mdics, whereas group ii, comprising c. glabrata, c. parapsilosis, c. guilliermondii, and c. krusei, had mics that we ... | 2005 | 16272484 |
| [a familial case of summer-type hypersensitivity pneumonitis possibly associated with bird breeder's lung diagnosed by bronchoalveolar lavage fluid]. | case 1: a 32-year-old woman had cough and exertional dyspnea in august 2002, and chest computed tomographic scan revealed diffuse centrilobular nodules. bronchoalveolar lavage fluid (balf) showed a high proportion of lymphocytes with a decreased cd 4/cd 8 ratio. transbronchial lung biopsy (tblb) specimens showed alveolitis. summer-type hypersensitivity pneumonitis was diagnosed on the basis of positive findings of anti-trichosporon antibodies in the serum. case 2: a 64-year-old man, the father o ... | 2005 | 16366370 |
| trichosporon asahii fatal infection in a non-neutropenic patient after orthotopic liver transplantation. | trichosporon asahii is a yeast that may cause systemic infection, especially in neutropenic patients. to our knowledge, only two cases of invasive infection with trichosporon were previously described in liver transplant recipients. we describe an additional case of t. asahii infection after orthotopic liver transplantation in a non-neutropenic patient who had no known risk factor for invasive fungal infection, and died in spite of amphotericin b therapy. | 2005 | 16390408 |
| breakthrough trichosporonosis in patients with hematologic malignancies receiving micafungin. | micafungin is a newly approved antifungal agent in the echinocandin class that is active against candida species and aspergillus species. however, this agent has limited activity against a number of fungi, including trichosporon species. we describe 4 patients who developed disseminated trichosporonosis during the use of micafungin. no cases of trichosporonosis had been seen in the 2 years prior to january 2003, when micafungin became available in our hospital. | 2006 | 16477548 |
| novel method for aroma recovery from the bioconversion of lutein to beta-ionone by trichosporon asahii using a mesoporous silicate material. | in this work, we report on the synthesis and ability of the mesoporous material mcm-41 to adsorb the norisoprenoid beta-ionone. this compound, with a violet aroma note, can be produced from lutein by the yeast trichosporon asahii through a bioconversion process. we found that beta-ionone inhibited the yeast growth and constrained aroma formation. growth inhibition was overcome using silicate mcm-41 as sorbent device in a fermentation system that allowed product removal from the culture medium by ... | 2006 | 16501971 |
| trichosporon asahii fungemia in a patient with non-hematological malignancy. | trichosporon fungemia is usually seen in neutropenic patients with underlying hematological malignancies. in this report we describe a fatal case of trichosporon asahii fungemia in a non-neutropenic patient with a non-hematological malignancy. for 1 week the patient exhibited hematuria, weakness, easy fatigability and headaches. at admission she had anemia, renal failure and evidence of right hydronephrosis and bladder wall masses as detected by ct scan. she did not have a history of tobacco abu ... | 2006 | 16632916 |
| cutaneous abscess by trichosporon asahii developing on a steroid injection site in a healthy adult. | we report a rare case of cutaneous abscess by trichosporon asahii in an immunocompetent adult. a 31-year-old korean woman presented to our hospital with a cutaneous abscess. she had received an intralesional steroid injection 4 months earlier on the site of a hypertrophic scar. direct sequencing of the intergenic spacer regions of the rrna genes identified t. asahii. the decreased local immunity after the steroid injection might have triggered the infection by t. asahii. a cutaneous abscess form ... | 2006 | 16716160 |
| efficacy of voriconazole in a guinea pig model of invasive trichosporonosis. | we have evaluated the efficacy of voriconazole (vrc) in a systemic infection by trichosporon asahii in immunosuppressed guinea pigs. vrc was more effective than amphotericin b in prolonging survival and reducing tissue burden. the best results were obtained with vrc at 10 mg/kg of body weight/day. | 2006 | 16723595 |
| [emerging deep-seated fungal infection, trichosporonosis]. | deep-seated trichosporonosis is a lethal opportunistic infection occasionally found in immunocompromised patients, particularly those who are neutropenic due to cytotoxic therapy for hematological malignancies. trichosporon asahii is considered the principal etiologic agent of non-candida fungemia and disseminated trichosporonosis in japan. this infection may disseminate to multiple organs and difficult to diagnosis and treat. because clinical findings and courses of trichosporonosis are similar ... | 2006 | 16780125 |
| successful treatment of breakthrough trichosporon asahii fungemia with voriconazole in a patient with acute myeloid leukemia. | we describe a 55-year-old man with acute myelogenous leukemia who developed breakthrough trichosporon asahii fungemia during 5 days of micafungin treatment. although the patient's clinical condition improved considerably after the start of voriconazole treatment, blood culture results remained positive for t. asahii for 3 days, and fever persisted for 7 days thereafter. the patient achieved complete hematological remission, and he received successful consolidation chemotherapy without developing ... | 2006 | 16838224 |
| [nosocomial infection due to trichosporon asahii: clinical revision of 22 cases]. | twenty two cases of nosocomial infection caused by trichosporon asahii, detected during a period of six years (1999-2005) is described. the patients were predominantly males with an average age of 47.3 years-old. the predominant diseases in the study group were respiratory insufficiency, cancer, diabetes, chronic renal insufficiency, cirrhosis and aids. the main predisposing conditions were antibiotic therapy, mechanical ventilation, urethral catheterization, catheter, corticoids, transplant, im ... | 2006 | 16854183 |
| trichosporon asahii. blood-stream infection in a non-cancer patient receiving combination antifungal therapy. | | 2006 | 16964817 |
| [investigation of some virulence factors of trichosporon asahii strains isolated from the clinical samples of hospitalized patients]. | in this study, acid proteinase, phospholipase and esterase activities, slime production and hydrophobic properties of a total of 48 trichosporon asahii strains isolated from urine (43), peritoneal fluid (1), tongue swab sample (1), blood (1), nephrostomy (1) and one saprophyte strain from nail sample, were investigated. in none of the 48 strains, acid proteinase and phospholipase activities could be detected, while all were found to be esterase positive. in hydrocarbon assay performed with n-hex ... | 2006 | 17001852 |
| biofilm formation by the emerging fungal pathogen trichosporon asahii: development, architecture, and antifungal resistance. | trichosporon asahii is the most common cause of fatal disseminated trichosporonosis, frequently associated with indwelling medical devices. despite the use of antifungal drugs to treat trichosporonosis, infection is often persistent and is associated with high mortality. this drove our interest in evaluating the capability of t. asahii to form a biofilm on biomaterial-representative polystyrene surfaces through the development and optimization of a reproducible t. asahii-associated biofilm model ... | 2006 | 17005804 |
| black piedra: report of a french case associated with trichosporon asahii. | | 2006 | 17040463 |
| disseminated, fatal trichosporon asahii infection in a bone marrow transplant recipient. | trichosporon asahii is the most important species regularly isolated from systemic mycoses and shows a predilection for hematogenous dissemination. this report describes the first fatal case of disseminated trichosporonosis caused by t. asahii in a patient with familial aplastic anemia (aa). an 11-year-old girl with familial aa received chemoradiotherapy and immunosuppressive therapy for bone marrow transplantation. she was neutropenic and suffered from fever, cough, and severe mouth ulcers. t. ... | 2006 | 17066207 |
| autopsy case of disseminated trichosporon inkin infection identified with molecular biological and biochemical methods. | trichosporon species are usually opportunistic pathogens. disseminated trichosporonosis is uncommon but is increasingly reported with a high mortality rate, especially in immunocompromised patients. although trichosporon asahii and t. mucoides are known as the most common pathogens of disseminated trichosporonosis, cases of systemic infection due to t. inkin have been reported recently. however, no autopsy case of disseminated t. inkin infection has been reported. herein is presented an autopsy ... | 2006 | 17096731 |
| superficial cutaneous trichosporon asahii infection in an immunocompetent host. | | 2006 | 17184246 |
| urinary tract infection by trichosporon asahii. | trichosporon asahii is a basidiomycetous yeast which causes white piedra and onychomycosis in immunocompetent hosts as well as various localized and disseminated invasive infections in immunodeficient hosts. urinary tract infection caused by trichosporon asahii is rare. one month after posterior urethral valve surgery a seven-month-old male child presented with fever, severe vomiting and crying on micturition for five--to six days. yeast-like fungus was isolated in pure cultures from three conse ... | 2006 | 17185852 |
| breakthrough infection of trichosporon asahii in a patient with chronic lymphocytic leukemia. | | 2007 | 17322000 |
| genotypic study of strains belonging to the genus trichosporon. | trichosporon asahii is an opportunistic fungus considered the leading etiologic agent of trichospornosis, a disease that causes great morbidity/mortality among affected patients. the identification of the etiologic agent is generally obtained through physiological and morphological studies. molecular investigations, such as species-specific primers (pcr), have recently been developed with the aim of applying a more simple, specific, and faster technology for mycological diagnosis. the genetic ma ... | 2007 | 17325944 |
| invasive trichosporonosis due to trichosporon asahii in a non-immunocompromised host: a rare case report. | a case of invasive trichosporonosis due to trichosporon asahii in an otherwise healthy young adult male presenting as meningoencephalitis and pneumonia is reported here. t. asahii was isolated from cerebrospinal fluid and sputum of the patient and morphologic forms of organism was also demonstrated on direct gram stain of sputum. the isolate was identified as t. asahii. the patient partially responded to fluconazole therapy. our case suggests that t. asahii can no longer be linked with trichospr ... | 2007 | 17377356 |
| genotype distribution of clinical isolates of trichosporon asahii based on sequencing of intergenic spacer 1. | the sequence polymorphisms of intergenic transcribed spacer and the antifungal susceptibility profile of 18 trichosporon asahii isolates from spain, argentina, and brazil together with 43 intergenic transcribed spacer 1 sequences deposited in the genbank were analyzed. six genotypes were detected instead of 5 genotypes described previously. genotype 1 was the most common found comprising 57.3% of all strains, followed by genotype 3 (14.7%) and genotype 5 (13.1%). spanish strains had members in a ... | 2007 | 17509802 |
| ce separation of proteins and yeasts dynamically modified by peg pyrenebutanoate with fluorescence detection. | the optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside peg pyrenebutanoate, were applied in cze and cief with the acidic gradient in ph range 2-5.5, both with fluorescence detection. peg pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. the narrow peaks of modified analytes were detected. the values of the pi's of the labeled proteins were calculated using new fluorescen ... | 2007 | 17557360 |
| real-time pcr assay to detect dna in sera for the diagnosis of deep-seated trichosporonosis. | deep-seated trichosporonosis due to trichosporon asahii is life-threatening and has high mortality. a real-time pcr assay to detect t. asahii dna in sera for diagnosis of this fungal infection was developed. the assay showed a higher sensitivity than polysaccharide antigen detection method. our new real-time pcr assay may be used for diagnosing deep-seated trichosporonosis due to t. asahii. | 2007 | 17579275 |
| trichosporon asahii as an emerging etiologic agent of disseminated trichosporonosis: a case report and an update. | to report a fatal case of disseminated trichosporonosis caused by trichosporon asahii in a patient with acute myeloblastic leukemia (aml) and to present an update on systemic trichosporonosis with special reference to india. | 2004 | 17642680 |
| [summer-type hypersensitivity pneumonitis-immunological examinations in onset and non-onset members]. | we examined immunological differences by non-specific and specific stimuli, using trichosporon asahii (ta), in 5 subjects in one family. the mother and her eldest daughter, among the 5 subjects in the family, were given diagnoses of summer-type hypersensitivity pneumonitis (hp) as a result of imaging, serological and histological examinations. the symptoms in both cases improved immediately after systemic corticosteroid therapy. their home environment was cleaned up, and there has been no recurr ... | 2007 | 17682469 |
| breakthrough infection of trichosporon asahii during posaconazole treatment in a patient with acute myeloid leukaemia. | a neutropenic patient with acute myeloid leukaemia experienced a breakthrough infection of trichosporon asahii during posaconazole treatment. after treatment was changed to a combination therapy with voriconazole and liposomal amphotericin b, the infection resolved. posaconazole works effectively as an antifungal prophylaxis and salvage therapy in rare invasive fungal infections. this case however illustrates that breakthrough infections with t. asahii may occur during posaconazole treatment. | 2007 | 17690928 |
| yeast diversity in rice-cassava fermentations produced by the indigenous tapirapé people of brazil. | the tapirapé people of the tapi'itãwa tribe of brazil produce several fermented foods and beverages, one of which is called 'cauim'. this beverage usually makes up the main staple food for adults and children. several substrates are used in its production, including cassava, rice, corn, maize and peanuts. a fermentation using rice and cassava was conducted, and samples were collected at 4-h intervals for microbial analysis. the yeast population was low at the beginning of the fermentation and re ... | 2007 | 17697080 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| breakthrough trichosporon asahii fungemia in neutropenic patient with acute leukemia while receiving caspofungin. | a 47-year-old man with newly diagnosed acute myeloblastic leukemia and non-insulin-dependent diabetes mellitus developed trichosporon asahii fungemia while receiving caspofungin as empirical antifungal therapy. the diagnosis was based on repeated isolation of t. asahii in culture of blood for three times. despite treatment with amphotericin b and voriconazole, the patient died. the in vitro antifungal susceptibilities of the t. asahii isolates were only available after the patient died. in vitro ... | 2008 | 17882360 |
| granuloma associated with trichosporon asahii infection in the lung: unusual pathological findings and pcr detection of trichosporon dna. | we describe the first case of a trichosporon asahii infection of the lung characterized by an epithelioid granuloma with multinucleated giant cells. the patient died of respiratory failure due to lung fibrosis and lung cancer. autopsy showed primary lung cancer with metastasis, lung fibrosis, and a focal infectious lesion containing the filamentous and yeast forms of a fungus. from its shape the fungus was judged to be trichosporon, but unusual pathological findings revealed an epithelioid granu ... | 2007 | 17885944 |
| successful treatment of septic shock with purpura fulminans caused by trichosporon asahii in an immunocompetent patient. | trichosporon asahii is an emerging mycosis characterized by high mortality rate in immunologically compromised patients. only a few cases have been reported in immunocompetent subjects. we report a 46-yr-old man who had been healthy and who presented with septic shock and purpura fulminans caused by trichosporon asahii. he responded well to antifungal therapy with amphotericin b and voriconazole. | 2007 | 18000295 |
| [identification and susceptibility against fluconazole and albaconazole of 100 yeasts' strains isolated from vaginal discharge]. | vulvovaginal candidiasis is a condition that affects a great number of fertile women. it is considered the second cause of genital infection after vaginosis due to gam complex. candida albicans is the most frequent isolated species from vaginal discharge. however, sometimes more than one yeast species could be found in the same clinical sample that are more resistant to antifungal drugs. nowadays, it is necessary to identify properly up to species level the isolated microorganism and to determin ... | 2007 | 18095765 |
| trichosporon asahii following polymicrobial infection in peritoneal dialysis-associated peritonitis. | | 2008 | 18178957 |
| [fungal infections of the lower urinary tracts in urological patients]. | to examine etiological structure of fungal infections of the lower urinary tract (luti) in urological patients. | 2007 | 18219974 |
| postoperative trichosporon asahii spondylodiscitis after open lumbar discectomy: a case report. | a case report of trichosporon asahii spondylodiscitis. | 2008 | 18277860 |
| recurrent trichosporon asahii glossitis: a case report. | this case report aims at describing an infection of the tongue as a manifestation of a trichosporon asahii infection, its association with bronchial asthma and steroid administration, and to present a review of the literature pertaining to its antifungal susceptibility profile. | 2008 | 18335127 |