| reclassification of brevibacterium oxydans (chatelain and second 1966) as microbacterium oxydans comb. nov. | phylogenetic and chemotaxonomic analyses indicate that brevibacterium oxydans is closely related to species of the genus microbacterium, namely microbacterium liquefaciens, microbacterium luteolum and microbacterium saperdae. dna-dna reassociation values of less than 60% between brevibacterium oxydans and these three microbacterium species support the distinctness of this misclassified brevibacterium species, which is reclassified as microbacterium oxydans comb. nov. | 1999 | 10028259 |
| biodegradation of cyclohexylamine by brevibacterium oxydans ih-35a. | a bacterial strain capable of growing on cyclohexylamine (cham) was isolated by using enrichment and isolation techniques. the strain isolated, strain ih-35a, was classified as a member of the genus brevibacterium. the results of growth and enzyme studies are consistent with degradation of cham via cyclohexanone (chnone), 6-hexanolactone, 6-hydroxyhexanoate, and adipate. cell extracts obtained from this strain grown on cham contained cham oxidase, and the model for cham oxidation by this enzyme ... | 1999 | 10224025 |
| catheter-related microbacterium bacteremia identified by 16s rrna gene sequencing. | we describe the application of 16s rrna gene sequencing in defining two cases of catheter-related microbacterium bacteremia. in the first case, a gram-positive bacillus was isolated from both the blood culture and central catheter tip of a 39-year-old woman with chronic myeloid leukemia. the api coryne system identified the isolate as 98.9% aureobacterium or corynebacterium aquaticum. in the second case, a gram-positive bacillus was recovered from five sets of blood cultures from both central ca ... | 2002 | 12089308 |
| bacteremia due to a novel microbacterium species in a patient with leukemia and description of microbacterium paraoxydans sp. nov. | a yellow-pigmented coryneform rod was isolated from the blood of a child with acute lymphoblastic leukemia who was perfused with a central venous catheter. the culture bottles were positive twice, at a 2-month interval. the isolate was identified as a microbacterium sp. and studied along with five other similar strains. phenotypic, chemotaxonomic, and genetic characteristics indicated that they are closely related to microbacterium oxydans but that they belong to a distinct species, for which th ... | 2003 | 12734292 |
| studies on cultured and uncultured microbiota of wild culex quinquefasciatus mosquito midgut based on 16s ribosomal rna gene analysis. | to describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. the culturable microbiota was identified as acinetobacter junii, ac. calcoaceticus, aeromonas culicicola, bacillus thuringiensis, microbac ... | 2004 | 15210998 |
| [microbacterium oxydans, a symbiont of djungarian hamster, displaying probiotic properties]. | a resident microorganism (strain kho-17) was isolated from secretions of the specific glandular structures at the angles of mouth of djungarian hamster (phodopus campbelli). according to cultural, morphological, and physiological properties as well as to the phylogenetic analysis basing on the sequences of 16s rrna gene and analysis of the cell wall the strain was assigned to the species microbacterium oxydans. the bacterium isolated displayed probiotic properties when administered orally as a s ... | 2004 | 15609853 |
| characterisation of the nitrile hydratase gene clusters of rhodococcus erythropolis strains aj270 and aj300 and microbacterium sp. aj115 indicates horizontal gene transfer and reveals an insertion of is1166. | the nitrile metabolising strains aj270, aj300 and aj115 were isolated from the same location. the strains have very similar nitrile metabolising profiles. sequencing of the 16s rrna gene indicates that strains aj270 and aj300 are novel strains of rhodococcus erythropolis while strain aj115 is a novel microbacterium strain very closely related to microbacterium oxydans and microbacterium liquefaciens. analysis of the structure of the nitrile hydratase/amidase gene clusters in the three strains in ... | 2005 | 15803388 |
| the selection of mixed microbial inocula in environmental biotechnology: example using petroleum contaminated tropical soils. | the impact of inorganic n and p additions on a tropical soil contaminated with petroleum hydrocarbons was investigated using molecular and culture techniques. microcosms were incubated for 42 days and sampled at 0, 1, 7, 28 and 42 days. changes in bacterial community structure were determined using denaturing gradient gel electrophoresis (dgge) of the rrna following reverse transcription pcr using primers specific to the v3 region of the 16s rrna gene. to identify which components of the microbi ... | 2006 | 16226327 |
| purification and characterization of a novel cyclohexylamine oxidase from the cyclohexylamine-degrading brevibacterium oxydans ih-35a. | cyclohexylamine oxidase (chao) from a cell extract of brevibacterium grown on cyclohexylamine was purified 50.2-fold, to electrophoretic homogeneity, by serial chromatographies. the molecular mass of the native enzyme was estimated to be approximately 50 kda by gel filtration and sds-page. the optimum ph was 7.4 and the stable ph range was 6.0 to 7.0. the enzyme was thermostable up to 30 degrees c. the enzyme was found to be highly specific for the deamination of alicyclic monoamines such as cyc ... | 1999 | 16232609 |
| microbacterium isolates from the vicinity of a radioactive waste depository and their interactions with uranium. | three oligotrophic bacterial strains were cultured from the ground water of the deep-well monitoring site s15 of the siberian radioactive waste depository tomsk-7, russia. they were affiliated with actinobacteria from the genus microbacterium. the almost fully sequenced 16s rrna genes of two of the isolates, s15-m2 and s15-m5, were identical to those of cultured representatives of the species microbacterium oxydans. the third isolate, s15-m4, shared 99.8% of 16s rrna gene identity with them. the ... | 2007 | 17381522 |
| conversion of puerarin into its 7-o-glycoside derivatives by microbacterium oxydans (cgmcc 1788) to improve its water solubility and pharmacokinetic properties. | microbacterium oxydans strain nj 6 isolated from soil samples converted puerarin into two novel compounds, puerarin-7-o-glucoside and puerarin-7-o-isomaltoside, via an unreported o-glycosylation of the phenolic hydroxyl group at the 7-position of puerarin. sucrose, maltotriose, and maltose could be used as glucosyl donors for glycosylation of puerarin, but uridine-diphosphate glucose, glucose, fructose, lactose, cyclodextrin, and starch could not. regardless of the position of b-ring in the (iso ... | 2008 | 18795283 |
| identities of microbacterium spp. encountered in human clinical specimens. | in the present study, 50 strains of yellow-pigmented gram-positive rods that had been isolated from human clinical specimens and collected over a 5-year period were further characterized by phenotypic and molecular genetic methods. all 50 strains belonged to the genus microbacterium, and together they represented 18 different species. microbacterium oxydans (n = 11), m. paraoxydans (n = 9), and m. foliorum (n = 7) represented more than half of the strains included in the present study. the isola ... | 2008 | 18799696 |
| detection and hazard assessment of pathogenic microorganisms in medical wastes. | this study was undertaken to investigate the types and concentrations of microbial agents in various medical wastes as well as to characterize their survivals in these wastes at different temperatures for microbial risk assessment. medical wastes collected from 5 major hospitals in south korea were classified and stored at three different temperatures (-20, 6, and 30 degrees c). presence of various microorganisms such as pathogenic viruses and bacteria were investigated by both cultivation and b ... | 2009 | 19827491 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| permeabilization of microbacterium oxylans shifts the conversion of puerarin from puerarin-7-o-glucoside to puerarin-7-o-fructoside. | the main product of the conversion of puerarin by unpermeabilized cells of bacterium microbacterium oxydans cgmcc 1788 was puerarin-7-o-glucoside (241 +/- 31.9 microm). permeabilization with 40% ethanol could not increase conversion yield, whereas it resulted in change of main product; a previous trace product became a main product (213 +/- 48.0 microm) which was identified as a novel puerarin-7-o-fructoside by electrospray ionization time-of-flight ms, (13)c nmr, (1)h nmr, and gc-ms analysis of ... | 2010 | 19943046 |
| characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses. | diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ... | 2011 | 20859703 |
| exogenous coproporphyrin iii production by corynebacterium aurimucosum and microbacterium oxydans in erythrasma lesions. | erythrasma is a superficial skin disease caused by gram-positive corynebacterium species. coral-red fluorescence under wood's light, strongly suggestive of erythrasma, can be attributed to the presence of porphyrins. fractionated porphyrin analysis in erythrasma lesions is yet to be reported. we attempted to investigate erythrasma lesions by isolating the responsible bacteria and determining their exogenous porphyrin production by hplc analysis. we observed a 78-year-old woman with erythrasma wh ... | 2011 | 21393451 |
| structural analysis of a novel cyclohexylamine oxidase from brevibacterium oxydans ih-35a. | cyclohexylamine oxidase (chao) is a flavoprotein first described in brevibacterium oxydans strain ih-35a that carries out the initial step of the degradation of the industrial chemical cyclohexylamine to cyclohexanone. we have cloned and expressed in escherichia coli the chao-encoding gene (chaa) from b. oxydans, purified chao and determined the structures of both the holoenzyme form of the enzyme and a product complex with cyclohexanone. chao is a 50 kda monomer with a phbh fold topology. it be ... | 2013 | 23555888 |
| serratia odorifera a midgut inhabitant of aedes aegypti mosquito enhances its susceptibility to dengue-2 virus. | mosquito midgut plays a crucial role in its vector susceptibility and pathogen interaction. identification of the sustainable microflora of the midgut environment can therefore help in evaluating its contribution in mosquito-pathogen interaction and in turn vector competence. to understand the bacterial diversity in the midgut of aedes aegypti mosquitoes, we conducted a screening study of the gut microbes of these mosquitoes which were either collected from fields or reared in the laboratory "cu ... | 2012 | 22848375 |
| serratia odorifera mediated enhancement in susceptibility of aedes aegypti for chikungunya virus. | the susceptibility of the mosquito to the invading pathogen is predominantly dictated by the complex interactions between the mosquito midgut and the surface proteins of the invading pathogen. it is well documented that the midgut microbiota plays an important role in determining the susceptibility of the mosquito to the pathogen. in the present study, we investigated the influence of serratia odorifera, an endogenous cultivable midgut inhabitant of aedes aegypti on the chikungunya virus (chikv) ... | 0 | 25027087 |
| experimental studies and kinetic modeling of the growth of phenol-degrading bacteria in turbulent fluids. | understanding the interaction between microorganisms and fluid dynamics is important for aquatic ecosystems, though only sporadic attention has been focused on this topic in the past. in this study, particular attention was paid to the phenol-degrading bacterial strains microbacterium oxydans ly1 and alcaligenes faecalis ly2 subjected to controlled fluid flow under laboratory conditions. these two strains were found to be able to degrade phenols over a concentration range from 50 to 500 mg/l und ... | 2016 | 27557974 |
| functional role of bacteria from invasive phragmites australis in promotion of host growth. | we hypothesize that bacterial endophytes may enhance the competitiveness and invasiveness of phragmites australis. to evaluate this hypothesis, endophytic bacteria were isolated from p. australis. the majority of the shoot meristem isolates represent species from phyla firmicutes, proteobacteria, and actinobacteria. we chose one species from each phylum to characterize further and to conduct growth promotion experiments in phragmites. bacteria tested include bacillus amyloliquefaciens a9a, achro ... | 2016 | 27260154 |
| [diversity and antimicrobial activities of actinomycetes from pesticide-contaminated spots in shandong peninsula]. | in order to study diversity and find antimicrobial activities of actinomycetes from pesticide-contaminated spots in shandong peninsula. | 2012 | 22799208 |
| distinct gene expression profile of xanthomonas retroflexus engaged in synergistic multispecies biofilm formation. | it is well known that bacteria often exist in naturally formed multispecies biofilms. within these biofilms, interspecies interactions seem to have an important role in ecological processes. little is known about the effects of interspecies interactions on gene expression in these multispecies biofilms. this study presents a comparative gene expression analysis of the xanthomonas retroflexus transcriptome when grown in a single-species biofilm and in dual- and four-species consortia with stenotr ... | 2017 | 27505346 |
| structural characterization of a novel antioxidant pigment produced by a photochromogenic microbacterium oxydans strain. | the microbacteriaceae family, such as microbacterium, is well known for its ability to produce carotenoid-type pigments, but little has been published on the structure of such pigments. here, we isolated the yellow pigment that is responsible for the yellowish color of a microbacterium oxydans strain isolated from a decomposing stump of a resinous tree. the pigment, which is synthesized when the bacterium is grown under light, was purified and characterized using several spectroscopic analyses, ... | 2016 | 27339186 |
| new recombinant cyclohexylamine oxidase variants for deracemization of secondary amines by orthogonally assaying designed mutants with structurally diverse substrates. | to further expand the substrate range of the cyclohexylamine oxidase (chao) from brevibacterium oxydans, a library of diverse mutants was created and assayed toward a group of structurally diverse substrates. among them, mutants t198a and m226a exhibited enhanced activity relative to wt chao for most (s)-enantiomers of primary amines and some secondary amines. while mutants t198i, l199i, l199f, m226i and m226t were more active than wt chao toward the primary amines, mutants t198f, l199t, y321a, ... | 2016 | 27138090 |
| isolation and growth kinetics of a novel phenol-degrading bacterium microbacterium oxydans from the sediment of taihu lake (china). | seven phylogenetically diverse phenol-degrading bacterial strains designated as p1 to p7 were isolated from the industry-effluent dump sites of an industrial area near taihu lake, china. through the 16s rdna sequence analysis, these strains were widely distributed among five different genera: rhodococcus (p1), pseudomonas (p2-p4), acinetobacter (p5), alcaligenes (p6), and microbacterium (p7). all seven isolates were capable of growing with phenol as the sole carbon source. strain p7 was found to ... | 2016 | 27120643 |
| screening of bacterial strains isolated from uranium mill tailings porewaters for bioremediation purposes. | the present work characterizes at different levels a number of bacterial strains isolated from porewaters sampled in the vicinity of two french uranium tailing repositories. the 16s rrna gene from 33 bacterial isolates, corresponding to the different morphotypes recovered, was almost fully sequenced. the resulting sequences belonged to 13 bacterial genera comprised in the phyla firmicutes, actinobacteria and proteobacteria. further characterization at physiological level and metals/metalloid tol ... | 2017 | 27068793 |
| a selective medium for the isolation of microbacterium species in oral cavities. | the genus microbacterium has been isolated from the environment, dairy goods, and human clinical specimens. although, in our previous studies, some microbacterium species were infrequently detected in oral samples collected from humans, there is currently no report that these organisms, which are capable of causing serious systemic infections, were isolated from the human oral cavity. the aim of the present study was to develop a selective medium to isolate the representative microbacterium spec ... | 2015 | 26149625 |
| microbacterium oxydans and microbacterium liquefaciens: a biological alternative for the treatment of ni-v-containing wastes. | the present study evaluated 15 isolates obtained of environmental samples capable of tolerating high ni and v concentrations. those coded as mnsh2-phgii-1, mnsh2-phgii-2 and mv-phgii-2 showed a minimum inhibitory concentration higher than 200 ppm for ni and v and showed removal percentages corresponding to 84, 75 and 26% for ni and 60, 55 and 20.3% for v, respectively, in liquid medium. when spent catalyst was added at 16% (w/v) pulp density, the highest ni and v removal corresponded to mnsh2-ph ... | 2015 | 25837562 |
| three new fusidic acid derivatives and their antibacterial activity. | two steroid acids, cephalosporin p1 and isocephalosporin p1, were isolated from hapsidospora irregularis ferm bp-2511. these compounds are structurally related to fusidic acid. their nmr data were completely assigned on the basis of the 2d nmr spectra. incubation of these two compounds with microbacterium oxydans cgmcc 1788 in luria-bertani broth yielded the same set of three new 3-dehydrogenated products, 3-keto-isocephalosporin p1, 3-keto-cephalosporin p1 and 6-deacetyl-3-keto-cephalosporin p1 ... | 2015 | 25824664 |
| characterization of a selenium-tolerant rhizosphere strain from a novel se-hyperaccumulating plant cardamine hupingshanesis. | a novel selenium- (se-) hyperaccumulating plant, cardamine hupingshanesis, accumulating se as a form of secys2, was discovered in enshi, hubei, china, which could not be explained by present selenocysteine methyltransferase (smt) theory. however, it is interesting to investigate if rhizosphere bacteria play some roles during secys2 accumulation. here, one se-tolerant rhizosphere strain, microbacterium oxydans, was isolated from c. hupingshanesis. phylogenetic analysis and 16s rrna gene sequences ... | 2014 | 25478582 |
| high prevalence of biofilm synergy among bacterial soil isolates in cocultures indicates bacterial interspecific cooperation. | biofilms that form on roots, litter and soil particles typically contain multiple bacterial species. currently, little is known about multispecies biofilm interactions and few studies have been based on environmental isolates. here, the prevalence of synergistic effects in biofilm formation among seven different soil isolates, cocultured in combinations of four species, was investigated. we observed greater biofilm biomass production in 63% of the four-species culture combinations tested than in ... | 2015 | 24936766 |
| complete genome sequence of a novel phage, vb_moxs-isf9, infecting methylotrophic microbacterium: first report of a virulent microbacterium phage. | here, we report the first genome sequence of a new virulent phage of microbacterium oxydans, termed vb_moxs-isf9, which was isolated from sewage. transmission electron microscopy showed that the isolated phage, which has a hexagonal head of about 80 nm in diameter and a long non-contractile tail of about 240 nm, belongs to the family siphoviridae. the vb_moxs-isf9 dna was completely sequenced and found to be 59,254 bp in length, with a g+c content of 62.76% and 120 putative open reading frames ( ... | 2014 | 24777828 |
| [effects of actinomycetes agent on ginseng growth and rhizosphere soil microflora]. | taking the ginseng in xiao xing' an mountains of northeast china as test object, this paper studied the effects of applying streptomyces pactum (act12) on ginseng growth and on the soil microflora in root zone and root surface. after treated with act12, the yield and quality of ginseng' s medicinal part improved, the induced enzyme activities in leaves and the root activity increased, and the numbers and proportions of soil bacteria and actinomycetes increased significantly while those of soil f ... | 2013 | 24380350 |
| microbacterium oxydans, a novel alginate- and laminarin-degrading bacterium for the reutilization of brown-seaweed waste. | there is a growing demand for the efficient treatment of seaweed waste. we identified six bacterial strains from the marine environment for the reutilization of brown-seaweed waste, and the most potentially useful strain, microbacterium oxydans, was chosen and further investigated. plate assays indicated that this bacterial isolate possessed both alginate lyase and laminarinase activities. the optimal inoculum size, ph, temperature and substrate concentration for the degradation of brown-seaweed ... | 2013 | 24076515 |
| bacteria immobilisation on hydroxyapatite surface for heavy metals removal. | selected bacterial strains were immobilised on the surface of hydroxyapatite (ca10(po4)6(oh)2 - hap) of natural origin (fish bones). the capacity of the material, alone and in combination with the bacterial strains to act as heavy metal removers from aqueous streams was assessed. pseudomonas fluorescens (s3x), microbacterium oxydans (ec29) and cupriavidus sp. (1c2) were chosen based on their resistance to heavy metals and capacity of adsorbing the metals. these systems were tested using solution ... | 2013 | 23524400 |
| high level multiple antibiotic resistance among fish surface associated bacterial populations in non-aquaculture freshwater environment. | freshwater fish, alburnus alburnus (bleak), were captured from lake mogan, situated in ankara, during spring. the surface mucus of the fish was collected and associated bacteria were cultured and isolated. by sequencing pcr-amplified 16s rna encoding genes, the isolates were identified as members of 12 different genera: acinetobacter, aeromonas, bacillus, brevundimonas, gordonia, kocuria, microbacterium, mycobacterium, pseudomonas, rhodococcus, and staphylococcus, in addition to one strain that ... | 2012 | 23039919 |