| mucus ph of the tiger puffer takifugu rubripes is an important factor for host identification by the monogenean heterobothrium okamotoi. | we examined a host-finding factor of the monogenean heterobothrium okamotoi oncomiracidia to develop an alternative prophylaxis. h. okamotoi oncomiracidia attached preferentially to gill filaments and skin mucus from the tiger puffer takifugu rubripes compared with corresponding material from other tested fishes (amber jack seriola dumerili, red sea bream pagrus major, japanese flounder paralichthys olivaceus and spotted halibut verasper variegatus). the body mucus ph of the tiger puffer was 6.4 ... | 2003 | 12964825 |
| two species of parasitic copepods (caligidae) new to japan. | two species of parasitic copepods (siphonostomatoida: caligidae), caligus sclerotinosus roubal, armitage & rohde, 1983, parasitic on red seabream pagrus major (temminck & schlegel), and lepeophtheirus longiventralis yü & wu, 1932, parasitic on spotted halibut verasper variegatus (temminck & schlegel), are redescribed based on material found on their respective hosts cultured in japan. both species are new to japan. preliminary observation on the occurrence of l. longiventralis indicates that the ... | 2004 | 14739672 |
| distribution of marine birnavirus in cultured marine fish species from kagawa prefecture, japan. | to determine the distribution of marine birnavirus (mabv) in cultured populations of different marine fish species, 1291 pooled tissue samples from 2672 fish belonging to 22 species and one hybrid were collected from kagawa prefecture, japan, during 1999-2001. using cell-culture mabv was isolated from three species: yellowtail, seriola quinqueradiata temminck & schlegel (positive number/sample number, 10/419), amberjack, s. dumerili (risso) (4/72), and japanese flounder, paralichthys olivaceus ( ... | 2004 | 15009250 |
| production of symmetrical flatfish by controlling the timing of thyroid hormone treatment in spotted halibut verasper variegatus. | the thyroid hormone plays an essential role in the metamorphosis in flatfish, during which external asymmetry (for example, eye relocation and pigmentation) is established. however, no information is available on the expression mechanisms of metamorphic asymmetry. since the presence of malformed juveniles (having either ocular or blind side characteristics on both sides) was known in spotted halibut (verasper variegatus), the effect of the timing of thyroid hormone treatment was investigated. wh ... | 2005 | 15748720 |
| response of neobenedenia girellae (monogenea) oncomiracidia to brightness and black-and-white contrast. | neobenedenia girellae, a capsalid monogenean, is a significant pathogen due to both its ability to cause high mortality in fishes and its low host specificity. established control methods have both advantages and disadvantages. biological control measures with no unfavourable effects on the environment should be incorporated into the control strategy. the response of n. girellae oncomiracidia to brightness and black-and-white contrast was investigated to search for an alternative approach of dis ... | 2007 | 17599782 |
| characterization of highly concentrated serum lectins in spotted halibut verasper variegatus. | mannose-binding lectins were purified from flatfish spotted halibut (verasper variegatus) serum. these lectins, which we named vvl (verasper variegatus lectin)-alpha (approximately 33 kda) and vvl-beta (approximately 30 kda) (vvls), under non-reducing sds-page, were surprisingly highly concentrated in serum (1.92+/-0.55 mg/ml; n=5), compared with other serum lectins. both vvls are heterodimers comprised of 2 types of subunit via inter-subunit disulfide bonds, and one subunit of vvl-alpha has a n ... | 2008 | 18076773 |
| lipid content and fatty acid composition of the monogenean neobenedenia girellae and comparison between the parasite and host fish species. | neobenedenia girellae, a capsalid monogenean, is a destructive fish parasite. we studied the lipid content and fatty acid composition of n. girellae and the skin and cutaneous mucus of a host fish, the amberjack seriola dumerili (carangidae). the lipid content of adult n. girellae was less than one-fourth that of both the skin and cutaneous mucus of its host. adult n. girellae, s. dumerili skin and mucus had a relatively high weight-percentage of c16:0, c18:1(n-9), c18:0 and c22:6(n-3) fatty aci ... | 2008 | 18598577 |
| development of polymorphic microsatellite markers in barfin flounder (verasper moseri) and spotted halibut (verasper variegatus) by the cross-species amplification. | barfin flounder (verasper moseri) and spotted halibut (verasper variegatus) are two commercially important flatfish species in the northeast asia. in the present study, we reported polymorphic microsatellite markers in v. moseri and v. variegatus by the cross-species amplification of microsatellite primers developed previously in two other related marine fish species. a total of 244 polymorphic microsatellite markers were selected for cross-species amplification in v. moseri and v. variegatus, o ... | 2010 | 21132381 |
| a hepatic metabolomics-based diagnostic approach to assess lethal toxicity of dithiocarbamate fungicide polycarbamate in three marine fish species. | the present study was performed to evaluate the toxic effect of the dithiocarbamate fungicide polycarbamate (pc) on the hepatic metabolic profiles of three marine fish species, red sea bream (pagrus major), spotted halibut (verasper variegatus), and marbled flounder (pleuronectes yokohamae). first, juvenile fish were exposed to graded concentrations of pc for 96h; the 96-h lc50 values obtained were 22-29, 239-553, and 301-364µgl(-1) for red sea bream, spotted halibut, and marbled flounder, respe ... | 2017 | 28011422 |
| isolation of novel microsatellite markers from paralichthys lethostigma (paralichthyidae) and their cross-species application in pleuronectiformes. | we investigated the genetic diversity of the southern flounder paralichthys lethostigma. microsatellite-enriched libraries were constructed and novel microsatellite markers were developed and applied for genetic detection of wild populations. cross-species amplification was also conducted in five pleuronectiforme species. of 45 randomly selected and sequenced clones, 43 contained a ca or ga repeat motif. fourteen pairs of primers were designed to investigate the polymorphism and genetic structur ... | 2013 | 24391024 |
| molecular cloning and differential expression of three gnrh genes during ovarian maturation of spotted halibut, verasper variegatus. | in this study, the gonadotropin-releasing hormone (gnrh) genes in spotted halibut were cloned and sequenced by isolating their cdnas. the species expressed three molecular forms of gnrh in the brain: chicken-type gnrh-ii (cgnrh-ii), seabream-type gnrh (sbgnrh), and salmon-type gnrh (sgnrh). phylogenetic analysis divided the molecular forms of gnrhs into three branches: cgnrh-ii branch, sgnrh branch, and fish-specific gnrh branch. the spatial expression showed that they had the highest expression ... | 2012 | 22674773 |