| in vitro encystation and excystation of blastocystis ratti. | cysts of blastocystis ratti were produced in vitro by culturing the parasite in iscove's modified dulbecco's medium (imdm) with increasing concentrations of horse serum. yields up to 3 x 10(6) cysts/ml of culture medium were obtained after 72 h. encystation efficiency was time, strain and inoculum size dependent. a viability of > 70% was determined by flow cytometry employing fluorescein diacetate and propidium iodide staining. the presence of chitin as a cyst wall component was demonstrated by ... | 1999 | 10028529 |
| blastocystis ratti induces contact-independent apoptosis, f-actin rearrangement, and barrier function disruption in iec-6 cells. | blastocystis is an enteric protozoan purportedly associated with numerous clinical cases of diarrhea, flatulence, vomiting, and other gastrointestinal symptoms. despite new knowledge of blastocystis cell biology, genetic diversity, and epidemiology, its pathogenic potential remains controversial. numerous clinical and epidemiological studies either implicate or exonerate the parasite as a cause of intestinal disease. therefore, the aim of this study was to investigate the pathogenic potential of ... | 2006 | 16790785 |
| blastocystis ratti contains cysteine proteases that mediate interleukin-8 response from human intestinal epithelial cells in an nf-kappab-dependent manner. | blastocystis is a ubiquitous enteric protozoan found in the intestinal tracts of humans and a wide range of animals. evidence accumulated over the last decade suggests association of blastocystis with gastrointestinal disorders involving diarrhea, abdominal pain, constipation, nausea, and fatigue. clinical and experimental studies have associated blastocystis with intestinal inflammation, and it has been shown that blastocystis has potential to modulate the host immune response. blastocystis is ... | 2008 | 18156286 |
| high prevalence of subtype 4 among isolates of blastocystis hominis from symptomatic patients of a health district of valencia (spain). | in order to know the genetic diversity of blastocystis hominis from a health district of valencia (spain) 51 clinical isolates from symptomatic patients, 31 axenic and 20 monoxenic, were ribotyped by analysing the restriction fragment length polymorphism (rflp) of amplicons obtained by polymerase chain reaction (pcr) of small-subunit of ribosomal dna genes (ssu-rdna). for this purpose, dna was subjected to two independent pcr (rd3-rd5, f1-r1) and to three independent treatments with restrictases ... | 2009 | 19471964 |
| blastocystis sp. and blastocystis ratti in a brazilian porcupine (coendou prehensilis) with diarrhea. | a hand-raised, 5-mo-old, intact male brazilian porcupine (coendou prehensilis) was evaluated for chronic diarrhea, failure to thrive, and anorexia. on presentation the porcupette was dull, dehydrated, and passing yellow, malodourous, watery diarrhea. cytologic examination of feces revealed a large number of organisms, morphologically consistent with blastocystis. blastocystis polymerase chain reaction (pcr) performed on feces was positive. direct sequencing on two sequential samples confirmed th ... | 2016 | 27468042 |