| [influence of oxygen and metabolic inhibitors upon both vegetative growth and development of mitochondria in peniophora gigantea (fr.) massee]. | | 1970 | 4100819 |
| purification and characterization of a pyranose oxidase from the basidiomycete peniophora gigantea and chemical analyses of its reaction products. | a pyranose oxidase was isolated from mycelium extracts of the basidiomycete peniophora gigantea. this enzyme was purified 104-fold to apparent homogeneity with a yield of about 75% by steps involving fractionated ammonium sulphate precipitation, chromatography on deae-sephacel, sephacryl s 300, s sepharose and q sepharose. the native pyranose oxidase has a relative molecular mass (m(r)) of 322,800 +/- 18,300 as determined on the basis of its stokes' radius (rs = 6.2 nm) and sedimentation coeffic ... | 1993 | 8319689 |
| southern blot screening for lignin peroxidase and aryl-alcohol oxidase genes in 30 fungal species. | screening to detect genes encoding lignin peroxidase (lip) and aryl-alcohol oxidase (aao) has been carried out with 30 fungal strain using dna probes from genes lpo of phanerochaete chrysosporium (encoding lip isoenzyme h8) and aao of pleurotus eryngii. evidence for the presence of genes closely related to lpo was found in bjerkandera adusta, fomes fomentarius, ganoderma applanatum, ganoderma australe, lentinula degener, peniophora gigantea, p. chrysosporium, phanerochaete flavido-alba and trame ... | 2000 | 11051421 |
| engineering of pyranose 2-oxidase from peniophora gigantea towards improved thermostability and catalytic efficiency. | to improve the stability and catalytic efficiency of pyranose 2-oxidase (p2ox) by molecular enzyme evolution, we cloned p2ox cdna by race-pcr from a cdna library derived from the basidiomycete peniophora gigantea. the p2ox gene was expressed in escherichia coli bl21(de3), yielding an intracellular and enzymatically active p2oxb with a volumetric yield of 500 units/l. site-directed mutagenesis was employed to construct the p2ox variant e540k (termed p2oxb1), which exhibited increased thermo- and ... | 2005 | 15660220 |
| direct analysis of ribosomal dna in denaturing gradients: application on the effects of phlebiopsis gigantea treatment on fungal communities of conifer stumps. | the aim of this study was to test the usefulness of direct pcr-amplification in analysing fungal diversity in stumps. the analysis was conducted on stumps treated against heterobasidion spp. using a commercial formulation of phlebiopsis gigantea (rotstop), and carried out using denaturing gradient gel electrophoresis (dgge) of small subunit (ssu) ribosomal dna (rdna) fragments pcr-amplified directly from wood dna samples using two separate fungus-specific primer pairs. on average, two (range 0-9 ... | 2005 | 15736868 |
| purification by immunoaffinity chromatography, characterization, and structural analysis of a thermostable pyranose oxidase from the white rot fungus phlebiopsis gigantea. | a moderately thermostable pyranose oxidase (prod) was purified to apparent homogeneity with a yield of 71% from mycelium extracts of the white rot fungus phlebiopsis gigantea by an efficient three-step procedure that included heat treatment, immunoaffinity chromatography, and gel filtration on superdex 200. prod of p. gigantea is a glycoprotein with a pi between ph 5.3 and 5.7. the relative molecular weight (m(infr)) of native prod is 295,600 (plusmn) 5% as determined by four independent methods ... | 1996 | 16535364 |
| biological processing of pine logs for pulp and paper production with phlebiopsis gigantea. | phlebiopsis gigantea (=phanerochaete gigantea) is a white rot fungus that rapidly colonizes cut stumps, stems, and branches of pine. two laboratory and several field studies showed that inoculation of red pine logs, pinus resinosa, with p. gigantea reduced the pitch content of wood, facilitated bark removal, modified wood cells, and controlled detrimental sapstain. isolations from inoculated logs revealed up to 100 and 80% colonization of the sapwood by p. gigantea after 8 weeks in the field and ... | 1997 | 16535609 |
| identification and analysis of differentially expressed cdnas during nonself-competitive interaction between phlebiopsis gigantea and heterobasidion parviporum. | the molecular factors regulating interspecific interaction between the saprotrophic biocontrol fungus phlebiopsis gigantea and the conifer pathogen heterobasidion parviporum were investigated. we constructed cdna libraries and used expressed sequence tag analysis for the identification and characterization of genes expressed during the self and nonself-hyphal interaction. cdna clones from either the pathogen or biocontrol agent were arrayed on nylon membrane filters and differentially screened w ... | 2006 | 16819947 |
| fungal biotransformation products of dehydroabietic acid. | dehydroabietic acid (dha) (1) is one of the main compounds in scots pine wood responsible for aquatic and microbial toxicity. the degradation of 1 by trametes versicolor and phlebiopsis gigantea in liquid stationary cultures was followed by hplc-dad-elsd. both fungi rapidly degraded dha relative to a control. more breakdown products were observed for t. versicolor than for p. gigantea. after 13 days, four compounds were identified by means of spectroscopic methods in p. gigantea cultures: 1beta- ... | 2007 | 17315956 |
| wood-decay fungi in fine living roots of conifer seedlings. | the mycorrhizal basidiomycetes are known to have multiple, independent evolutionary origins from saprotrophic ancestors. to date, a number of studies have revealed functional resemblance of mycorrhizal fungi to free-living saprotrophs, but information on the ability of saprotrophic fungi to perform as mycorrhizal symbionts is scarce. here, the objective was to investigate the ability of three wood-decay fungi, phlebiopsis gigantea, phlebia centrifuga and hypholoma fasciculare, to colonize fine r ... | 2007 | 17388906 |
| bioreactor for solid-state cultivation of phlebiopsis gigantea. | phlebiopsis gigantea fungus used in biological control of root rot is currently cultivated commercially in disposable, sterilizable plastic bags. a novel packed bed bioreactor was designed for cultivating p. gigantea and compared to the plastic bag method and to a tray bioreactor. the spore viability of 5.4 x 10(6) c.f.u./g obtained with the packed bed bioreactor was of the same order of magnitude as the viabilities obtained with the other cultivation methods. furthermore, the packed bed bioreac ... | 2008 | 17891458 |
| molecular characterisation of two novel double-stranded rna elements from phlebiopsis gigantea. | the incomplete sequences of two large, 10-12 kbp, double-stranded rnas (dsrnas) found in the tw-2 isolate of the saprophytic fungus, phlebiopsis gigantea (pg) are reported. both pgv-tw2 dsrna1 and dsrna2 potentially encode fusion proteins which are apparently expressed by a translational frameshifting mechanism. the c-terminal region of both predicted proteins was 21% identical and contained the eight motifs conserved in rna-dependent rna polymerases of dsrna mycoviruses and had highest similari ... | 2009 | 19430898 |
| use of a breeding approach for improving biocontrol efficacy of phlebiopsis gigantea strains against heterobasidion infection of norway spruce stumps. | sixty-four wild heterokaryotic isolates of phlebiopsis gigantea were analysed for asexual spore production, growth rate and competitive ability against heterobasidion in vitro, as well as growth rate in norway spruce wood. these p. gigantea traits were considered important for controlling infection of norway spruce stumps by spores of heterobasidion spp. ten most promising p. gigantea isolates were crossed with each other and 172 f(1) progeny heterokaryons were analysed for the above-mentioned t ... | 2009 | 19496817 |
| plant-feeding insects harbor double-stranded rna viruses encoding a novel proline-alanine rich protein and a polymerase distantly related to that of fungal viruses. | novel double-stranded rnas (approximately 8 kbp) were isolated from threecornered alfalfa hopper (spissistilus festinus) and beet leafhopper (circulifer tenellus), two plant-feeding hemipteran insect pests. the two new viruses, designated spissistilus festinus virus 1 (spfv1) and circulifer tenellus virus 1 (citv1), do not appear to be encapsidated in conventional virions and shared a genome organization similar to that of several unclassified fungal viruses. spfv1 and citvl encode a proline-ala ... | 2010 | 20541786 |
| conifer root and butt rot caused by heterobasidion annosum (fr.) bref. s.l. | summary the root and butt rot caused by heterobasidon annosum is one of the most destructive diseases of conifers in the northern temperate regions of the world, particularly in europe. economic losses attributable to heterobasidion infection in europe are estimated at 800 million euros annually. the fungus has been classified into three separate european intersterile species p (h. annosum), s (h. parviporum) and f (h. abietinum) based on their main host preferences: pine, spruce and fir, respec ... | 2005 | 20565666 |
| improved molecular methods to characterise serpula lacrymans and other basidiomycetes involved in wood decay. | early detection of indoor wood-decay fungi is crucial to prevent building deterioration and thereby avoid considerable economic loss. due to their increased sensitivity, two reliable dna-based fingerprinting techniques, capillary electrophoresis single-strand conformation polymorphism (ce-sscp) and denaturing high-performance liquid chromatography (dhplc), were used to identify serpula lacrymans and to profile wood-rot basidiomycetes in the built environment. molecular fungal diversity was asses ... | 2010 | 21146565 |
| amplification of mitochondrial small subunit ribosomal dna of polypores and its potential for phylogenetic analysis. | there has been a systematic need to seek adequate phylogenetic markers that can be applied in phylogenetic analyses of fungal taxa at various levels. the mitochondrial small subunit ribosomal dna (mt ssu rdna) is generally considered to be one of the molecules that are appropriate for phylogenetic analyses at a family level. in order to obtain universal primers for polypores of hymenomycetes, mt ssu rrna genes were cloned from bjerkandera adusta, ganoderma lucidum, phlebiopsis gigantea, and phel ... | 2002 | 21156556 |
| characterization of a novel dsrna element in the pine endophytic fungus diplodia scrobiculata. | diplodia scrobiculata and diplodia pinea are endophytic fungi associated with dieback and cankers of mainly pinus spp. in many parts of the world. these two fungi are closely related and have, in the past, been considered to represent two morphological forms (a and b morphotypes) of d. pinea. dsrna elements are known to occur in both d. scrobiculata and d. pinea. two dsrna elements from d. pinea, ssrv1 and ssrv2, have been characterized previously. the aim of this study was to characterize a thi ... | 2011 | 21442227 |
| response of living tissues of pinus sylvestris to the saprotrophic biocontrol fungus phlebiopsis gigantea. | the saprotrophic fungus phlebiopsis gigantea has been used for several years as a biocontrol agent against the conifer pathogen heterobasidion annosum. although the effectiveness of p. gigantea in biocontrol has been shown empirically, the long-term effect on living conifer trees as well as the mechanism underlying its antagonistic activity is still unknown. an additional concern is the potential of p. gigantea to acquire a necrotrophic habit through adaptation to living wood tissues. by using a ... | 2011 | 21551358 |
| incidence of phlebiopsis gigantea large virus-1 in a collection of phlebiopsis gigantea isolates. | eighty six phlebiopsis gigantea isolates from at least 9 different tree species from various locations in 12 different european countries and north america were screened for the presence of large molecular weight dsrna >10-ákbp in size. in 7 isolates, which contained large dsrnas, the presence of phlebiopsis gigantea large virus-1 (pglv-1) was suggested following the sequencing of the rt-pcr amplicons generated with pglv-1 specific oligonucleotide primers which also revealed little genetic diver ... | 2011 | 21818645 |
| The pathogenic white-rot fungus Heterobasidion parviporum triggers non-specific defence responses in the bark of Norway spruce. | Norway spruce [Picea abies (L.) Karst.] is one of the economically most important conifer species in Europe. The major pathogen on Norway spruce is Heterobasidion parviporum (Fr.) Niemelä & Korhonen. To achieve a better understanding of Norway spruce's defence mechanisms, transcriptional responses in bark to H. parviporum infection were compared with the response to wounding using cDNA-amplified fragment length polymorphism. The majority of the recovered transcript-derived fragments (TDFs) showe ... | 2011 | 22084022 |
| sesquiterpenes from the conifer root rot pathogen heterobasidion occidentale. | investigation of the production of secondary metabolites of heterobasidion occidentale led to the isolation and identification of six sesquiterpenes (illudolone a and b, illudolactone a and b, deoxyfomannosin a and b) along with the well-known sesquiterpene fomannosin and the previously described benzohydrofuran fomannoxin. the structures and relative configurations of the compounds were determined by 1d and 2d nmr spectroscopic analysis as well as by hrms. their absolute configuration and biosy ... | 2012 | 22831894 |
| cypome of the conifer pathogen heterobasidion irregulare: inventory, phylogeny, and transcriptional analysis of the response to biocontrol. | the molecular mechanisms underlying the interaction of the pathogen, heterobasidion annosum s.l., the conifer tree and the biocontrol fungus, phlebiopsis gigantea have not been fully elucidated. members of the cytochrome p450 (cyp) protein family may contribute to the detoxification of components of chemical defence of conifer trees by h. annosum during infection. additionally, they may also be involved in the interaction between h. annosum and p. gigantea. a genome-wide analysis of cyps in hete ... | 2017 | 28089047 |
| molecular characterization of a new monopartite dsrna mycovirus from mycorrhizal thelephora terrestris (ehrh.) and its detection in soil oribatid mites (acari: oribatida). | a novel dsrna virus was identified in the mycorrhizal fungus thelephora terrestris (ehrh.) and sequenced. this virus, named thelephora terrestris virus 1 (ttv1), contains two reading frames in different frames but with the possibility that orf2 could be translated as a fusion polyprotein after ribosomal -1 frameshifting. picornavirus 2a-like motif, nudix hydrolase, phytoreovirus s7, and rdrp domains were found in a unique arrangement on the polyprotein. a new genus named phlegivirus and containi ... | 2016 | 26700067 |
| molecular characterization of a novel mycovirus from rhizoctonia fumigata ag-ba isolate c-314 baishi. | the complete genome sequence of a novel dsrna virus isolated from rhizoctonia fumigata ag-ba isolate c-314 baishi (designated as rhizoctonia fumigata virus 1, rfv1) was determined. the rfv1 genome was 9,907 bp in length and contained two open reading frames (orfs). orf1 potentially coded for a 198.10-kda protein (p1). p1 shared low but significant amino acid sequence similarity to the putative protein encoded by lentinula edodes mycovirus (lev) orf1. p1 contained a nudix domain, which was also p ... | 2015 | 26133296 |
| increased delignification by white rot fungi after pressure refining miscanthus. | pressure refining, a pulp making process to separate fibres of lignocellulosic materials, deposits lignin granules on the surface of the fibres that could enable increased access to lignin degrading enzymes. three different white rot fungi were grown on pressure refined (at 6 bar and 8 bar) and milled miscanthus. growth after 28 days showed highest biomass losses on milled miscanthus compared to pressure refined miscanthus. ceriporiopsis subvermispora caused a significantly higher proportion of ... | 2015 | 25864034 |
| analysis of the phlebiopsis gigantea genome, transcriptome and secretome provides insight into its pioneer colonization strategies of wood. | collectively classified as white-rot fungi, certain basidiomycetes efficiently degrade the major structural polymers of wood cell walls. a small subset of these agaricomycetes, exemplified by phlebiopsis gigantea, is capable of colonizing freshly exposed conifer sapwood despite its high content of extractives, which retards the establishment of other fungal species. the mechanism(s) by which p. gigantea tolerates and metabolizes resinous compounds have not been explored. here, we report the anno ... | 2014 | 25474575 |
| evolutionary analysis of hydrophobin gene family in two wood-degrading basidiomycetes, phlebia brevispora and heterobasidion annosum s.l. | hydrophobins are small secreted cysteine-rich proteins that play diverse roles during different phases of fungal life cycle. in basidiomycetes, hydrophobin-encoding genes often form large multigene families with up to 40 members. the evolutionary forces driving hydrophobin gene expansion and diversification in basidiomycetes are poorly understood. the functional roles of individual genes within such gene families also remain unclear. the relationship between the hydrophobin gene number, the geno ... | 2013 | 24188142 |
| identification of novel double-stranded rna mycoviruses of fusarium virguliforme and evidence of their effects on virulence. | virulence and double-stranded rna (dsrna) profiles of 44 isolates of fusarium virguliforme were compared. when grouped according to dsrna profiles, isolates with large dsrnas were significantly (p≤0.05) less virulent than isolates without dsrnas. high-throughput sequence analysis of total rna prepared from cultures with large dsrnas identified two novel rna viruses with genome sequences of approximately 9.3 kbp, which were named fusarium virguliforme dsrna mycovirus 1 and fusarium virguliforme d ... | 2014 | 24009061 |
| expression analysis of the impact of culture filtrates from the biocontrol agent, phlebiopsis gigantea on the conifer pathogen, heterobasidion annosum s.s. transcriptome. | phlebiopsis gigantea has been routinely used as the biological control agent for the conifer pathogen heterobasidion annosum sensu lato, but the actual mechanism for the biocontrol process is not known. to investigate the effect of secreted molecules from culture filtrate produced by p. gigantea on the gene expression profile of h. annosum s.s., microarray analysis was used. analysis of the differentially expressed genes led to the identification of genes with diverse functions. a major proporti ... | 2013 | 23812104 |
| protoilludane sesquiterpenes from the wood decomposing fungus granulobasidium vellereum (ellis & cragin) jülich. | the secondary metabolites of the saprotrophic wood-decay basidiomycete fungus granulobasidium vellereum were studied. six sesquiterpenes were obtained; 2-hydroxycoprinolone (1), 8-deoxy-4a-hydroxytsugicoline (2), 8-deoxydihydrotsugicoline (3), which were previously not described, radulone a and b, and coprinolone ketodiol. additionally, base-treatment of 1 yielded the diagnostic degradation products 1a and 1b, whereas radulone a was found to form 4 under mild acidic conditions. the structures we ... | 2013 | 23517685 |
| the primary module in norway spruce defence signalling against h. annosum s.l. seems to be jasmonate-mediated signalling without antagonism of salicylate-mediated signalling. | a key tree species for the forest industry in europe is norway spruce [picea abies (l.) karst.]. one of its major diseases is stem and butt rot caused by heterobasidion parviporum (fr.) niemelä & korhonen, which causes extensive revenue losses every year. in this study, we investigated the parallel induction of norway spruce genes presumably associated with salicylic acid- and jasmonic acid/ethylene-mediated signalling pathways previously observed in response to h. parviporum. relative gene expr ... | 2013 | 23223898 |
| controlled feeding of hydrogen peroxide as oxygen source improves production of 5-ketofructose from l-sorbose using engineered pyranose 2-oxidase from peniophora gigantea. | 5-keto-d-fructose is a useful starting material for the synthesis of pyrrolidine iminosugars. it can be prepared by regioselective oxidation of l-sorbose using pyranose 2-oxidase (p2ox) and o(2) as a cosubstrate. as the solubility of o(2) in aqueous solution is low and the affinity of p2ox for o(2) is poor, we developed a new and efficient process for the production of 5-keto-d-fructose based on engineered p2ox from peniophora gigantea and in situ generation of o(2) from h(2) o(2) with catalase. ... | 2012 | 22684857 |
| differential expression of two hydrophobin genes (pgh1 and pgh2) from the biological control agent phlebiopsis gigantea. | phlebiopsis gigantea has been widely used as the biocontrol fungus against the root and butt rot disease of conifers caused by heterobasidion annosum. we investigated the regulation of two hydrophobin genes (pgh1 and pgh2) in strong and weak antagonistic isolates of the biological control agent p. gigantea under diverse substrate conditions. transcript abundance of pgh1 was higher in single cultures of strong performing isolates than in the weak performing isolates at the early and late stages o ... | 2012 | 22559922 |
| occurrence and impact of the root-rot biocontrol agent phlebiopsis gigantea on soil fungal communities in picea abies forests of northern europe. | the aim of this study was to assess belowground occurrence, persistence and possible impact of the biocontrol agent phlebiopsis gigantea (fr.) jülich on soil fungi. sampling of soil and roots of picea abies (l.) h. karst. was carried out at 12 p. gigantea-treated and five nontreated control sites representing 1- to 60-month-old clear-cuts and thinned forest sites in finland and latvia. the 454-sequencing of its rrna from fine roots, humus and mineral soil resulted in 8626 high-quality fungal seq ... | 2012 | 22443512 |
| molecular characterization of a novel mycovirus in the cultivated mushroom, lentinula edodes. | in the 1970s, mycoviruses were identified that infected the edible mushroom lentinula edodes (shiitake), but they were not regarded as causal agents for mushroom diseases. none of their genes has been sequenced. in this study, the dsrna genome of a mycovirus recently found in a shiitake commercial strain was sequenced and its molecular structure was characterized. | 2012 | 22390839 |