Publications

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isolation and characterization of gas vesicles from microcyclus aquaticus.intact gas vesicles of microcyclus aquaticus s1 were isolated by using centrifugally accelerated flotation of vesicles and molecular sieve chromatography. isolated gas vesicles were cylindrical organelles with biconical ends and measured 250x100nm. the gas vesicle membrane was composed almost entirely of protein; neither lipid nor carbohydrate was detected, although one mole of phosphate per mole of protein was found. amino acid analysis indicated that the protein contained 54.6% hydrophobic ami ...1977403898
ribosomal ribonucleic acid cistron homologies among hyphomicrobium and various other bacteria.the extent of hybrid formation between the ribosomal ribonucleic acid (r-rna) of hyphomicrobium strain b-522 and deoxyribonucleic acid (dna) from bacteria of 21 different genera was examined. three generalized groupings were formed. group i (72-100%) consisted entirely of other strains of hyphomicrobium. representatives of the genera rhodopseudomonas, chromatium, caulobacter, prosthecomicrobium, rhodomicrobium, hyphomonas, and hyphomicrobium made up group ii (49-69%). the remaining gram-negative ...1977861853
fatty acid composition of selected prosthecate bacteria.the cellular fatty acid composition of 14 strains of caulobacter speices and types, two species of prosthecomicrobium, and two species of asticcacaulis was determined by gas-liquid chromatography. in most of these bacteria, the major fatty acids were octadecenoic acid (c18:1), hexadecenoic acid (c16:1) and hexadecanoic acid (c16:0). some cyclopropane and branched chain fatty acids were detected in addition to the straight chained acids. hydroxytetradecanoic acid was an important component of p.e ...19761015941
[physiological and biochemical characteristics of the prosthecate bacteria, stella humosa and prosthecomicrobium sp]. 19744464304
prosthecomicrobium and ancalomicrobium: new prosthecate freshwater bacteria.direct microscopic examination of natural freshwater samples reveals a variety of small microorganisms having elaborate cellular appendages. several strains have been isolated from crude cultures containing low concentrations of organic nutrients. all of the isolates are procaryotic. they are aerobic chemoorganotrophs that require vitamins for growth. because they cannot be assigned to any of the existing bacterial genera, two new genera are proposed: ancalomicrobium for organisms which have sev ...19684870285
incidence of prosthecate bacteria in a polluted stream.water samples were collected aseptically several times throughout the year at nine stations on the red cedar river, a stream flowing through farmland and receiving effluent from several municipalities in central michigan. total prosthecate bacteria were enumerated by both direct and viable counting techniques. by direct techniques, these bacteria accounted for 0.62 to 1.1% of the total microflora during the study. the predominant type of appendaged bacteria was the caulobacters (caulobacter, ast ...19714943266
deoxyribonucleic acid base sequence homologies of some budding and prosthecate bacteria.the genetic relatedness of a number of budding and prosthecate bacteria was determined by deoxyribonucleic acid (dna) homology experiments of the direct binding type. strains of hyphomicrobium sp. isolated from aquatic habitats were found to have relatedness values ranging from 9 to 70% with strain "ea-617," a subculture of the hyphomicrobium isolated by mevius from river water. strains obtained from soil enrichments had lower values with ea-617, ranging from 3 to 5%. very little or no homology ...19725018022
rupture of the cell envelope by induced intracellular gas phase expansion in gas vacuolate bacteria.using a new approach, we estimated the physical strength of the cell envelopes of three species of gram-negative, gas vacuolate bacteria (microcyclus aquaticus, prosthecomicrobium pneumaticum, and meniscus glaucopis). populations of cells were slowly (0.5 to 2.9 h) saturated with argon, nitrogen, or helium to final pressures up to 100 atm (10, 132 kpa). the gas phases of the vesicles remained intact and, upon rapid (1 to 2 s) decompression to atmospheric pressure, expanded and ruptured the cells ...19807204336
kaistia adipata gen. nov., sp. nov., a novel alpha-proteobacterium.a taxonomic study was carried out on chj404t, a bacterial strain isolated from a soil sample collected in an industrial stream near the chung-ju industrial complex in korea. the strain was a gram-negative, aerobic, short rod to coccus-shaped bacterium. it grew well on nutrient agar medium and utilized a broad spectrum of carbon sources. the g+c content of the dna was 67.4 mol% and the major composition of ubiquinone was q-10. the major fatty acid was c18:1. comparative 16s rdna studies showed a ...200415747229
high numbers of prosthecate bacteria in pulp mill waste aeration lagoons.prosthecate bacteria comprised 0.6 to 10.5% of the bacterial community in samples from 11 pulp mill waste aeration lagoons. because of their distinct morphology, the genera ancalomicrobium, caulobacter, prosthecobacter, prosthecomicrobium, stella, and hyphomicrobium or hyphomonas could be identified and enumerated by direct microscopic examination. monthly samples from one lagoon showed that several genera varied from undetectable to predominant among the appendaged organisms. temperature (seaso ...197916345371
pseudoxanthobacter soli gen. nov., sp. nov., a nitrogen-fixing alphaproteobacterium isolated from soil.a gram-type negative or variable, aerobic, rod-shaped, nitrogen-fixing bacterium, designated strain cc4(t), was isolated on nutrient agar from a soil sample collected at a regional agricultural research field station located in kaohsiung county, taiwan. 16s rrna gene sequence analysis demonstrated that this isolate is unique, showing <92.5 % similarity with respect to species of the genera xanthobacter (maximum of 92.2 % similarity), prosthecomicrobium (92.0 %), devosia (91.9 %), kaistia (91.9 % ...200818599697
reclassification of the polyphyletic genus prosthecomicrobium to form two novel genera, vasilyevaea gen. nov. and bauldia gen. nov. with four new combinations: vasilyevaea enhydra comb. nov., vasilyevaea mishustinii comb. nov., bauldia consociata comb. nov. and bauldia litoralis comb. nov.species of the genus prosthecomicrobium are noted for their numerous cellular appendages or prosthecae that extend from the cells. this investigation confirms that the genus is polyphyletic based on an extensive analysis of the 16s rrna gene sequences of several named species of the genus. the analyses indicate that some prosthecomicrobium species are more closely related to non-prosthecate genera, including devosia, labrenzia, blastochloris, methylosinus, mesorhizobium and kaistia, than they ar ...201020118292
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