| characterization of a new picorna-like virus, himetobi p virus, in planthoppers. | picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy laodelphax striatellus fallen. the virus particles had a buoyant density of 1.352 g/ml in cscl and a sedimentation coefficient of 161 s. the virus capsid proteins consisted of three major polypeptides of m(r)s 36,500, 33,000 and 28,000, and three minor polypeptides. the virus contained a major ssrna of m(r) 2.8 x 10(6) and was also frequently associated with a minor dsrna of m(r) 4 x 10(6). the 3' end of the s ... | 1992 | 1634869 |
| determining the nucleotide sequence and capsid-coding region of himetobi p virus: a member of a novel group of rna viruses that infect insects. | we determined the complete genome sequence of himetobi p virus (hipv), an insect picorna-like virus, which was isolated from the small brown planthopper, laodelphax striatellus. the genome of hipv consists of 9,275 nucleotides excluding the poly (a) tail, and contains two large open reading frames (orfs), which were separated by a 176-nucleotide noncoding region. the deduced amino acid sequence of the first orf contains core motifs of picornaviral helicase, protease, and rna-dependent rna polyme ... | 1999 | 10550677 |
| nucleotide sequence analysis of triatoma virus shows that it is a member of a novel group of insect rna viruses. | triatoma virus (trv) is the only virus described to date that infects triatomines, and has previously been considered to be a member of the family picornaviridae on the basis of physico-chemical properties. the genome of trv was sequenced completely (9010 nt). analysis of the sequence revealed the presence of two large open reading frames (orfs). the predicted amino acid sequence of orf1 (nt 549-5936) showed significant similarity to the non-structural proteins of several animal and plant rna vi ... | 2000 | 10725445 |
| analysis of the complete genome sequence of black queen-cell virus, a picorna-like virus of honey bees. | a virus with picorna-like biophysical properties was isolated from south african honey bees. on the basis of serology, it was identified as an isolate of black queen-cell virus (bqcv). nucleotide sequence analysis revealed an 8550 nt polyadenylated genome containing two large orfs. the 5'-proximal orf (orf 1) represented 4968 nt while the 3'-proximal orf (orf 2) represented 2562 nt. the orfs were separated by a 208 nt intergenic region and were flanked by a 657 nt 5'-untranslated region and a 15 ... | 2000 | 10900051 |
| analysis of the complete genome sequence of acute bee paralysis virus shows that it belongs to the novel group of insect-infecting rna viruses. | the complete genome sequence of acute bee paralysis virus (abpv) was determined. the 9470 nucleotide, polyadenylated rna genome encoded two open reading frames (orf1 and orf2), which were separated by 184 nucleotides. the deduced amino acid sequence of the 5' orf1 (nucleotides 605 to 6325) showed significant similarity to the rna-dependent rna polymerase, helicase, and protease domains of viruses from the picornavirus, comovirus, calicivirus, and sequivirus families, as well as to a novel group ... | 2000 | 11080493 |
| a tertiary structure model of the internal ribosome entry site (ires) for methionine-independent initiation of translation. | cricket paralysis-like viruses have a dicistronic positive-strand rna genome. these viruses produce capsid proteins through internal ribosome entry site (ires)-mediated translation. the ires element of one of these viruses, plautia stall intestine virus (psiv), forms a pseudoknot immediately upstream from the capsid coding sequence, and initiates translation from other than methionine. previously, we estimated that the ires element of psiv consists of seven stem-loops using the program mfold; ho ... | 2001 | 11233983 |
| nucleotide sequence of 3'-end of the genome of taura syndrome virus of shrimp suggests that it is related to insect picornaviruses. | taura syndrome disease, caused by taura syndrome virus (tsv), is one of the most important viral diseases of penaeid shrimp in the western hemisphere resulting in catastrophic disease epidemics in farmed shrimp. we have cloned and sequenced a 3278 bp cdna representing the 3' end of the tsv genome. sequence analyses revealed that frame + 2 had the longest open reading (orf) frame. this frame contained a 5'-terminal 19 non-coding bases followed by an orf from nucleotides 20 to 3053 (encoding 1011 ... | 2001 | 11448031 |
| characterization of a novel satellite virus and a strain of himetobi p virus (dicistroviridae) from the brown planthopper, nilaparvata lugens. | two distinct spherical virus-like particles were purified from the brown planthopper, nilaparvata lugens. one was a geographical isolate of himetobi p virus (cripavirus, dicistroviridae). the other was 30 nm in diameter and contained positive-stranded rna. the rna was 1647 nucleotides in length and encoded only its own capsid protein, indicating that this particle is a satellite virus. transmission tests showed that the satellite was transmitted vertically; however, its helper virus was unknown. ... | 2006 | 16310214 |
| molecular analysis of capsid protein of homalodisca coagulata virus-1, a new leafhopper-infecting virus from the glassy-winged sharpshooter, homalodisca coagulata. | a new virus that infects and causes increased mortality in leafhoppers was isolated from the glassy-winged sharpshooter, homalodisca coagulata (say) (hemiptera: cicadellidae). the virus, named homalodisca coagulata virus -1, hocv-1, was associated with increased mortality of cultured 5(th) instar h. coagulata. to identify the presence of h. coagulata viral pathogens, cdna expression libraries were made from adult and nymphs. analysis using reverse transcriptase pcr demonstrated that the virus wa ... | 2006 | 19537993 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| novel dicistrovirus from bat guano. | a novel dicistrovirus (strain nb-1/2011/hun, kj802403) genome was detected from guano collected from an insectivorous bat (species pipistrellus pipistrellus) in hungary, using viral metagenomics. the complete genome of nb-1 is 9136 nt in length, excluding the poly(a) tail. nb-1 has a genome organization typical of a dicistrovirus with multiple 3b(vpg) and a cripavirus-like intergenic region (igr)-ires. nb-1 shares only 41 % average amino acid sequence identity with capsid proteins of himetobi p ... | 2014 | 25168044 |
| unusual characteristics of dicistrovirus-derived small rnas in the small brown planthopper, laodelphax striatellus. | in this study, sequences of small rna (srna) libraries derived from the insect vector laodelphax striatellus were assembled into contigs and used as queries for database searches. a large number of contigs were highly homologous to the genome sequence of an insect dicistrovirus, himetobi p virus (hipv). interestingly, hipv-derived srnas had a wide size distribution, and were relatively abundant throughout the 18-30 nt size range with only a slight peak at 22 nt. hipv srnas had a strong bias towa ... | 2014 | 24323637 |
| identification of himetobi p virus in the small brown planthopper by deep sequencing and assembly of virus-derived small interfering rnas. | profiling and assembly of virus-derived small interfering rnas (sirnas) using next-generation sequencing technologies have been very useful for identification and diagnosis of a number of plant and invertebrate viruses. in this work, we have conducted high-throughput pyrosequencing and bioinformatic analysis of the small brown planthopper (sbph, laodelphax striatellus), and these analyses unexpectedly showed that the himetobi p virus (hipv) was present in our laboratory cultures. hipv was also f ... | 2014 | 24239755 |