Publications

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isolation and preliminary characterization of temperature-sensitive mutants of lumbo virus. 1978619489
[rna polymerase activity associated with a bunya virus (lumbo)].rna dependent rna polymerase has been demonstrated in purified lumbo virus (bunyavirus) which contains a single stranded segmented rna. divalent cations (mn++ and mg++) are required for optimal in vitro activity. reaction products can be specifically annealed with the viral genome.1975808281
[demonstration of circular ribonucleic acid in the lumbo virus (bunyavirus)].electron microscopy study of rna extracted from lumbovirus shows the existence of circular molecules, which are not susceptible to thermal or chemical denaturation. these facts suggest that the rna is present inside the virion in a covalently closed circular form.1976820471
studies on lumbo virus replication. ii. properties or viral ribonucleoproteins and characterization of messenger rnas. 1976936468
three-segment rna genome of lumbo virus (bunyavirus). 19734210363
the s rna genome segments of batai, cache valley, guaroa, kairi, lumbo, main drain and northway bunyaviruses: sequence determination and analysis.bunyaviruses have a genome comprising three segments of negative-sense rna. the smallest rna segment, s, encodes the nucleocapsid protein, n, and a nonstructural protein, nss, in overlapping reading frames. the sequences of the s genome rna segments of seven bunyaviruses (batai, cache valley, guaroa, kairi, main drain, northway and lumbo) were determined from cloned cdnas obtained using a one-step reverse transcription-pcr protocol. these sequences were compared to those of six viruses previousl ...19948126455
inkoo and tahyna, the european california serogroup bunyaviruses: sequence and phylogeny of the s rna segment.inkoo (ink) and tahyna (tah) viruses, european representatives of the california serogroup (cal), genus bunyavirus, family bunyaviridae, are transmitted by mosquitoes and frequently infect man. the s segments of ink and tah prototype strains were amplified, cloned and sequenced. ink s consists of 986 and tah s of 977 nucleotides (nt) coding for a nucleocapsid protein of 235 amino acids (aa) and, in an overlapping reading frame, for a nonstructural protein of 92 or 97 aa, respectively. by s segme ...19968760424
the bunyamwera virus nonstructural protein nss inhibits viral rna synthesis in a minireplicon system.the small (s) genomic segment of bunyamwera virus (family bunyaviridae, genus bunyavirus) encodes the nucleocapsid protein, n, and a nonstructural protein, nss, in overlapping reading frames. in order to elucidate the function of nss, we established a plasmid-based minireplicon system using mammalian cells that express large amounts of t7 rna polymerase. expression of n, the viral polymerase protein (l), and a minireplicon containing a reporter gene was sufficient to reconstitute functional viru ...200111222097
studies on lumbo virus replication. i. rna-dependent rna polymerase associated with virions. 19761896
isolation of viruses from mosquitoes collected at lumbo, mozambique. i. lumbo virus, a new virus isolated from aedes (skusea) pembaensis theobald. 196214034311
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