| transfer of several phytopathogenic pseudomonas species to acidovorax as acidovorax avenae subsp. avenae subsp. nov., comb. nov., acidovorax avenae subsp. citrulli, acidovorax avenae subsp. cattleyae, and acidovorax konjaci. | dna-rrna hybridizations, dna-dna hybridizations, polyacrylamide gel electrophoresis of whole-cell proteins, and a numerical analysis of carbon assimilation tests were carried out to determine the relationships among the phylogenetically misnamed phytopathogenic taxa pseudomonas avenae, pseudomonas rubrilineans, "pseudomonas setariae," pseudomonas cattleyae, pseudomonas pseudoalcaligenes subsp. citrulli, and pseudomonas pseudoalcaligenes subsp. konjaci. these organisms are all members of the fami ... | 1992 | 1371056 |
| [isolation of protoplasts of xanthomonas rubrilineans and their use in the study of localization of aminopeptidases]. | the culture of xanthomonas rubrilineans was able to synthesize a number of intracellular aminopeptidases. to study localization of the enzymes in the cells, a protoplasting procedure was developed providing the yield of 99.7 per cent. the following subcellular fractions were isolated: periplasmic, cytoplasmic and membranous. it was shown that alanine aminopeptidase was a cytoplasmic enzyme and glutamate peptidase was a membrane-bound enzyme. | 1992 | 1417307 |
| [relation between the morphogenesis of xanthomonas rubrilineans and biosynthesis of aminopeptidase in the process of growth and development of its producer]. | the dynamics of growth and development of xanthomonas rubrilineans, a culture producing intracellular aminopeptidase, was studied. a difference between the growth rate determined by intensity of the total biomass accumulation and the rate of the culture multiplication was found. the difference was due to the presence of two phases in the culture development during the exponential growth: the phase of increasing the linear sizes of the cells and the phase of the culture intensive multiplication. ... | 1992 | 1417308 |
| [intracellular aminopeptidase from xanthomonas rubrilineans, hydrolyzing alpha-amino acid esters and cefalexin]. | the aminopeptidase was isolated from cell-free extracts of xanthomonas rubrilineans by protein precipitation by isopropyl ester with subsequent purification by affinity chromatography on cabs-sepharose, bacitracin-sepharose, gel filtration through sephadex g-200 and ultrafiltration, the total yield being 32% with 2200-fold purification. the enzyme was homogeneous during sds-paag electrophoresis. apart from the broad spectrum of the peptidase activity, aminopeptidase possesses a hydrolase activit ... | 1990 | 2096952 |
| r-body-producing bacteria. | until 10 years ago, r bodies were known only as diagnostic features by which endosymbionts of paramecia were identified as kappa particles. they were thought to be limited to the cytoplasm of two species in the paramecium aurelia species complex. now, r bodies have been found in free-living bacteria and other paramecium species. the organisms now known to form r bodies include the cytoplasmic kappa endosymbionts of p. biaurelia and p. tetraurelia, the macronuclear kappa endosymbionts of p. cauda ... | 1989 | 2651865 |
| sensitivity of pseudomonas rubrilineans to antibiotics and fungicides. | | 1973 | 4804334 |
| transformation of pbr322-derived plasmids in phytopathogenicpseudomonas avenae and enhanced transformation in itsproline-auxotrophic mutant | efficient transformation of pbr322 and its derivedplasmids, which have been widely used as cloning vectors in escherichiacoli, was observed in pseudomonas avenae (k1), the pathogen ofleaf blight disease in cereals. moreover, there was a 10- to 50-foldtransformation efficiency (1.3-3.0 x 10(6)/&mgr;g dna) in theproline-auxotrophic mutant (pr47), whose virulence to rice seedlingsdecreased. similar enhancement of the frequency of transfer by mobilizationof rsf1010, a broad host range plasmid, was o ... | 1997 | 9009065 |
| [biosynthesis of leucine aminopeptidase by xanthomonas rubrilineans 67 in culture with various concentrations of nitrogen containing compounds]. | the dynamics of consumption of amine and ammonium nitrogen and glucose in the process of xanthomonas rubrilineans 67 growth and biosynthesis of leucine aminopeptidase was studied. it was shown that the rate of leucine, alanine and glycine consumption as a source of amine nitrogen out of 16 amino acids was the highest during the fermentation. addition of these three amino acids or their mixtures to the medium at definite stages of the fermentation process increased the leucine aminopeptidase bios ... | 1998 | 9777099 |
| [hydrolase from xanthomonas rubrilineans for synthesis of cefalexin]. | the use of peptide hydrolase (ec 3.4.13.1) from xanthomonas rubrilineans for synthesis of the antibiotic cephalexin from 7-aminodesacetoxycephalosporanic acid was studied. the optimum conditions for production of cephalexin were determined, and the yield exceeded 80%. a method for monitoring the synthesis of this antibiotic synthesis by means of a conventional amino acid analyzer is proposed. | 2000 | 10867949 |
| flagellin from an incompatible strain of pseudomonas avenae induces a resistance response in cultured rice cells. | the host range of pseudomonas avenae is wide among monocotyledonous plants, but individual strains can infect only one or a few host species. the resistance response of rice cells to pathogens has been previously shown to be induced by a rice-incompatible strain, n1141, but not by a rice-compatible strain, h8301. to clarify the molecular mechanism of the host specificity in p. avenae, a strain-specific antibody that was raised against n1141 cells and then absorbed with h8301 cells was prepared. ... | 2000 | 10922369 |
| characterization of maize cytochrome p450 monooxygenases induced in response to safeners and bacterial pathogens. | plants use a diverse array of cytochrome p450 monooxygenases in their biosynthetic and detoxification pathways. to determine the extent to which various maize p450s are induced in response to chemical inducers, such as naphthalic anhydride (na), triasulfuron (t), phenobarbital, and bacterial pathogens (erwinia stuartii, acidovorax avenae), we have analyzed the response patterns of seven p450 transcripts after treatment of seedlings with these inducers. each of these p450 transcripts has distinct ... | 2001 | 11161067 |
| method for qualifying microbial removal performance of 0.1 micron rated filters. part i: characterization of water isolates for potential use as standard challenge organisms to qualify 0.1 micron rated filters. | although 0.1 microm rated filters intended for pharmaceutical sterilization applications have been commercially available for at least 15 years, there is no industry-wide standard for qualifying the microbial removal performance of these filters. in this article, we report on the bacterial challenge methodology used to screen four bacterial species for potential utility as a standard challenge organism to qualify 0.1 microm rated filters. these isolates were, in their natural state, demonstrated ... | 2001 | 11766822 |
| relationships within the proteobacteria of plant pathogenic acidovorax species and subspecies, burkholderia species, and herbaspirillum rubrisubalbicans by sequence analysis of 16s rdna, numerical analysis and determinative tests. | sequence data for 16s rdna of the type strains of acidovorax avenae subsp. avenae, a. avenae subsp. cattleyae, a. avenae subsp. citrulli, a. konjaci and herbaspirillum rubrisubalbicans were compared with genbank library accessions of burkholderia spp., comamonas sp., ralstonia solanacearum and variovorax sp. maximum parsimony analysis produced two clusters: 1. acidovorax spp., comamonas sp., and variovorax sp. (all in the comamonadaceae), and 2. burkholderia spp., ralstonia solanacearum, and her ... | 2001 | 11827206 |
| flagellin from an incompatible strain of acidovorax avenae mediates h2o2 generation accompanying hypersensitive cell death and expression of pal, cht-1, and pbz1, but not of lox in rice. | acidovorax avenae causes a brown stripe disease in monocot plants. we recently reported that a rice-incompatible strain of a. avenae caused hypersensitive cell death in rice and that the flagellin of the incompatible strain was involved in this response. the incompatible strain induced the rapid generation of h2o2 accompanying hypersensitive cell death and the expression of defense genes such as pal, cht-1, pbz1, and lox, whereas the compatible strain did not. the purified incompatible flagellin ... | 2003 | 12744513 |
| rice cdna microarray-based gene expression profiling of the response to flagellin perception in cultured rice cells. | incompatible strains of acidovorax avenae elicit an immune response in cultured rice cells, with immunity specifically induced by the flagellin of the incompatible strain. to identify genes regulated by flagellin perception signaling in cultured rice cells, gene expression patterns were analyzed with rice cdna microarrays, including 3,353 independent rice cdna clones. in all, 131 genes were differentially expressed between incompatible and compatible interactions. k-means clustering showed that ... | 2004 | 15384489 |
| [hydrolysis of peptides by immobilized bacterial peptide hydrolases]. | the feasibility of hydrolysis of a mixture of peptides with an enzyme from the bacterium xanthomonas rubrilineans, displaying a peptidase activity and immobilized on aluminum oxide, was studied. kinetic schemes and equations allowing for approaching quantitative description of peptide hydrolysis in complex mixtures containing free amino acids and peptides were obtained. it was demonstrated that as a result of hydrolysis, the content of free amino acids in hydrolysates decreased 2.5- to 3-fold an ... | 2004 | 15455716 |
| degradation of chlorobenzenes by a strain of acidovorax avenae isolated from a polluted aquifer. | a subsurface microbial community was isolated from a polluted site of suquía river (córdoba-argentina), acclimated during 15 days in aerobic conditions using 1,2-dichlorobenzene (1,2-dcb) as the sole carbon source. from this acclimated community, we isolated and identified by 16s rdna analysis a strain of acidovorax avenae, which was able to perform the complete biodegradation of 1,2-dcb in two days affording stoichiometric amounts of chloride. this pure strain was also tested for biodegradation ... | 2005 | 16157172 |
| expression of a bacterial flagellin gene triggers plant immune responses and confers disease resistance in transgenic rice plants. | flagellin is a component of bacterial flagella and acts as a proteinaceous elicitor of defence responses in organisms. flagellin from a phytopathogenic bacterium, acidovorax avenae strain n1141, induces immune responses in suspension-cultured rice cells. to analyse the function of flagellin in rice, we fused the n1141 flagellin gene to the cauliflower mosaic virus 35s promoter and introduced it into rice. many of the resulting transgenic rice plants accumulated flagellin at various levels. the t ... | 2008 | 18705865 |
| role of blossoms in watermelon seed infestation by acidovorax avenae subsp. citrulli. | abstract the role of watermelon blossom inoculation in seed infestation by acidovorax avenae subsp. citrulli was investigated. approximately 98% (84/87) of fruit developed from blossoms inoculated with 1 x 10(7) or 1 x 10(9) cfu of a. avenae subsp. citrulli per blossom were asymptomatic. using immunomagnetic separation and the polymerase chain reaction, a. avenae subsp. citrulli was detected in 44% of the seed lots assayed, despite the lack of fruit symptoms. furthermore, viable colonies were re ... | 2003 | 18942974 |
| biological control to protect watermelon blossoms and seed from infection by acidovorax avenae subsp. citrulli. | abstract the efficacy of biological control seed treatments with pseudomonas fluorescens (a506), acidovorax avenae subsp. avenae (aaa 99-2), and an unidentified gram-positive bacterium recovered from watermelon seed (ws-1) was evaluated for the management of bacterial fruit blotch (bfb) of watermelon. in growth chamber and greenhouse experiments, seed treated with aaa 99-2 displayed superior disease suppression, reducing bfb transmission by 96.5%. aaa 99-2, p. fluorescens a506, and kocide also s ... | 2005 | 18943044 |
| investigating intraspecific variation of acidovorax avenae subsp. citrulli using dna fingerprinting and whole cell fatty acid analysis. | abstract to assess the diversity of acidovorax avenae subsp. citrulli, 121 strains from watermelon, cantaloupe, and pumpkin were compared using pulse field gel electrophoresis of spei-digested dna and gas chromatographic analysis of fatty acid methyl esters. twenty-nine unique dna fragments resulted from dna digestion, and 14 distinct haplotypes were observed. based on cluster analysis, two subgroups, i and ii, were recognized, which accounted for 84.8% (eight haplotypes) and 15.2% (six haplotyp ... | 2000 | 18944608 |
| analysis of flagellin perception mediated by flg22 receptor osfls2 in rice. | plants have sensitive perception systems that recognize various pathogen-derived molecules. we previously reported that rice detects flagellin from a rice-incompatible strain of gram-negative phytopathogenic bacterium, acidovorax avenae, which induces subsequent immune responses involving cell death. the mechanism of flagellin perception in rice, however, has remained obscure. in this study, we found that flg22, a peptide derived from the flagellin n-terminus, induced weak immune responses witho ... | 2008 | 18986259 |
| reclassification of subspecies of acidovorax avenae as a. avenae (manns 1905) emend., a. cattleyae (pavarino, 1911) comb. nov., a. citrulli schaad et al., 1978) comb. nov., and proposal of a. oryzae sp. nov. | the bacterium acidovorax avenae causes disease in a wide range of economically important monocotyledonous and dicotyledonous plants, including corn, rice, watermelon, anthurium, and orchids. genotypic and phenotypic relatedness among strains of phytopathogenic a. avenae subsp. avenae, a. avenae subsp. citrulli, a. avenae subsp. cattleyae and a. konjaci, as well as all other acidovorax species, including a. facilis, the type strain of acidovorax, was determined. the 16s rdna sequencing confirmed ... | 2008 | 18993005 |
| simultaneous detection of acidovorax avenae subsp. citrulli and didymella bryoniae in cucurbit seedlots using magnetic capture hybridization and real-time polymerase chain reaction. | to improve the simultaneous detection of two pathogens in cucurbit seed, a combination of magnetic capture hybridization (mch) and multiplex real-time polymerase chain reaction (pcr) was developed. single-stranded dna hybridization capture probes targeting dna of acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, and didymella bryoniae, causal agent of gummy stem blight, were covalently attached to magnetic particles and used to selectively concentrate template dna from c ... | 2009 | 19453225 |
| phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control. | to study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. | 2009 | 19486410 |
| type iv pili are required for virulence, twitching motility, and biofilm formation of acidovorax avenae subsp. citrulli. | acidovorax avenae subsp. citrulli is the causal agent of bacterial fruit blotch (bfb), a threatening disease of watermelon, melon, and other cucurbits. despite the economic importance of bfb, relatively little is known about basic aspects of the pathogen's biology and the molecular basis of its interaction with host plants. to identify a. avenae subsp. citrulli genes associated with pathogenicity, we generated a transposon (tn5) mutant library on the background of strain m6, a group i strain of ... | 2009 | 19589067 |
| multilocus sequence typing reveals two evolutionary lineages of acidovorax avenae subsp. citrulli. | acidovorax avenae subsp. citrulli, causal agent of bacterial fruit blotch, has caused considerable damage to the watermelon and melon industry in china and the united states. understanding the emergence and spread of this pathogen is important for controlling the disease. to build a fingerprinting database for reliable identification and tracking of strains of a. avenae subsp. citrulli, a multilocus sequence typing (mlst) scheme was developed using seven conserved loci. the study included 8 orig ... | 2009 | 19594310 |
| implanted-port-catheter-related sepsis caused by acidovorax avenae and methicillin-sensitive staphylococcus aureus. | acidovorax avenae is a gram-negative rod in the family comamonadaceae and a phytopathogen found in the environment. human infections caused by members of the comamonadaceae are extremely rare. we report a case of implanted-port-catheter-related sepsis caused by acidovorax avenae and methicillin (meticillin)-sensitive staphylococcus aureus (mssa). | 2009 | 19710279 |
| acrylic acid removal from synthetic wastewater and industrial wastewater using ralstonia solanacearum and acidovorax avenae isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber. | ralstonia solanacearum and acidovorax avenae were isolated from a wastewater treatment system manufactured with polyacrylonitrile fiber. the investigation goal is to elucidate the effectiveness of ralstonia solanacearum and acidovorax avenae in treating acrylic acid from synthetic wastewater and industrial wastewater. the results reveal that ralstonia solanacearum and acidovorax avenae could utilize acrylic acid from synthetic wastewater for growth, when the initial acrylic acid concentration wa ... | 2009 | 19934523 |
| electron microscopic analysis of the h(2)o(2) accumulation preceding hypersensitive cell death induced by an incompatible strain of pseudomonas avenae in cultured rice cells. | summary infection of cultured rice cells with an incompatible strain of pseudomonas avenae induces a hypersensitive reaction of the host, while compatible strain infection produces no such reaction. the induction of h(2)o(2) generation in cultured rice cells by the incompatible strain of p. avenae precedes cell death. to examine the distribution of h(2)o(2) generation sites, cultured rice cells were incubated following infection with a cerium solution. detection of the reaction product, ce(oh)(2 ... | 2002 | 20569303 |
| development of surface plasmon resonance imaging for detection of acidovorax avenae subsp. citrulli (aac) using specific monoclonal antibody. | an immunosensor based on surface plasmon resonance imaging (spr imaging) using a specific monoclonal antibody 11e5 (mab 11e5) was developed for the detection of the seed-borne bacterium acidovorax avenae subsp. citrulli (aac), which causes fruit blotch in watermelons and cantaloupes, and compared to the conventional elisa technique. the 1:40 mixed self-assembled monolayer (mixed sam) surface was used for the immobilized mab 11e5 on sensor surface for the detection of aac. both whole cells and br ... | 2010 | 21093249 |
| new detection systems of bacteria using highly selective media designed by smart: selective medium-design algorithm restricted by two constraints. | culturing is an indispensable technique in microbiological research, and culturing with selective media has played a crucial role in the detection of pathogenic microorganisms and the isolation of commercially useful microorganisms from environmental samples. although numerous selective media have been developed in empirical studies, unintended microorganisms often grow on such media probably due to the enormous numbers of microorganisms in the environment. here, we present a novel strategy for ... | 2011 | 21304596 |
| rapid on-site detection of acidovorax avenae subsp. citrulli by gold-labeled dna strip sensor. | acidovorax avenae subsp. citrulli (aac) is one of the most harmful diseases in cucurbit production. a rapid and sensitive dna strip sensor was constructed based on gold nanoparticle-labeled oligonucleotide probes for the detection of aac. both the qualitative and semi-quantitative detections of target dna were successfully achieved using the developed dna strip sensor. the qualitative limit of detection (lod) of the strip sensor was determined as 4nm. the lod for the semi-quantitative detection ... | 2011 | 21536424 |
| glycosylation regulates the specific induction of rice immune responses by acidovorax avenae flagellin. | plants have a sensitive system that detects various pathogen-derived molecules to protect against infection. flagellin, a main component of the bacterial flagellum, from the rice avirulent n1141 strain of gram-negative phytopathogenic bacterium, acidovorax avenae, induces plant immune responses including h(2)o(2) generation, while flagellin from the rice virulent k1 strain of a. avenae does not induce these immune responses. to clarify the molecular mechanism that leads to these differing respon ... | 2011 | 21628471 |
| genome sequence of the rice-pathogenic bacterium acidovorax avenae subsp. avenae rs-1. | acidovorax avenae subsp. avenae is a phytobacterium which is the causative agent of several plant diseases with economic significance. here, we present the draft genome sequence of strain rs-1, which was isolated from rice shoots in a rice field in china. this strain can cause bacterial stripe of rice. | 2011 | 21742879 |
| transcriptome analysis of acidovorax avenae subsp. avenae cultivated in vivo and co-culture with burkholderia seminalis. | response of bacterial pathogen to environmental bacteria and its host is critical for understanding of microbial adaption and pathogenesis. here, we used rna-seq to comprehensively and quantitatively assess the transcriptional response of acidovorax avenae subsp. avenae strain rs-1 cultivated in vitro, in vivo and in co-culture with rice rhizobacterium burkholderia seminalis r456. results revealed a slight response to other bacteria, but a strong response to host. in particular, a large number o ... | 2014 | 25027476 |
| simultaneous detection of three bacterial seed-borne diseases in rice using multiplex polymerase chain reaction. | burkholderia glumae (bacterial grain rot), xanthomonas oryzae pv. oryzae (bacterial leaf blight), and acidovorax avenae subsp. avenae (bacterial brown stripe) are major seedborne pathogens of rice. based on the 16s and 23s rdna sequences for a. avenae subsp. avenae and b. glumae, and transposase a gene sequence for x. oryzae pv. oryzae, three sets of primers had been designed to produce 402 bp for b. glumae, 490 bp for x. oryzae, and 290 bp for a. avenae subsp. avenae with the 63°c as an optimum ... | 2016 | 27904465 |
| differential expression of in vivo and in vitro protein profile of outer membrane of acidovorax avenae subsp. avenae. | outer membrane (om) proteins play a significant role in bacterial pathogenesis. in this work, we examined and compared the expression of the om proteins of the rice pathogen acidovorax avenae subsp. avenae strain rs-1, a gram-negative bacterium, both in an in vitro culture medium and in vivo rice plants. global proteomic profiling of a. avenae subsp. avenae strain rs-1 comparing in vivo and in vitro conditions revealed the differential expression of proteins affecting the survival and pathogenic ... | 2012 | 23166741 |
| glycan moiety of flagellin in acidovorax avenae k1 prevents the recognition by rice that causes the induction of immune responses. | abstract recognition of pathogen-associated molecular patterns (pamps) such as flagellin, a main component of the bacterial flagellum, constitutes the first layer of plant immunity and is referred to as pamp-triggered immunity (pti). the rice avirulent n1141 strain of gram-negative phytopathogenic bacterium, acidovorax avenae, induces pti including h2o2 generation, while flagellin from the rice virulent k1 strain of a. avenae does not induce these immune responses. mass spectrometry analyses rev ... | 2014 | 25482815 |
| frameshift mutation confers function as virulence factor to leucine-rich repeat protein from acidovorax avenae. | many plant pathogens inject type iii (t3ss) effectors into host cells to suppress host immunity and promote successful infection. the bacterial pathogen acidovorax avenae causes brown stripe symptom in many species of monocotyledonous plants; however, individual strains of each pathogen infect only one host species. t3ss-deleted mutants of a. avenae k1 (virulent to rice) or n1141 (virulent to finger millet) caused no symptom in each host plant, suggesting that t3ss effectors are involved in the ... | 2016 | 28101092 |
| 3d structure modeling of alpha-amino acid ester hydrolase from xanthomonas rubrilineans. | alpha-amino acid ester hydrolase (ec 3.1.1.43, aeh) is a promising biocatalyst for the production of semi-synthetic β-lactam antibiotics, penicillins and cephalosporins. the aeh gene from xanthomonas rubrilineans (xraeh) was recently cloned in this laboratory. the three-dimensional structure of xraeh was simulated using the homology modeling method for rational design experiments. the analysis of the active site was performed, and its structure was specified. the key amino acid residues in the a ... | 2013 | 24455184 |
| the novel kasugamycin 2'-n-acetyltransferase gene aac(2')-iia, carried by the incp island, confers kasugamycin resistance to rice-pathogenic bacteria. | kasugamycin (ksm), a unique aminoglycoside antibiotic, has been used in agriculture for many years to control not only rice blast caused by the fungus magnaporthe grisea but also rice bacterial grain and seedling rot or rice bacterial brown stripe caused by burkholderia glumae or acidovorax avenae subsp. avenae, respectively. since both bacterial pathogens are seed-borne and cause serious injury to rice seedlings, the emergence of ksm-resistant b. glumae and a. avenae isolates highlights the urg ... | 2012 | 22660700 |
| gene expression of type vi secretion system associated with environmental survival in acidovorax avenae subsp. avenae by principle component analysis. | valine glycine repeat g (vgrg) proteins are regarded as one of two effectors of type vi secretion system (t6ss) which is a complex multi-component secretion system. in this study, potential biological roles of t6ss structural and vgrg genes in a rice bacterial pathogen, acidovorax avenae subsp. avenae (aaa) rs-1, were evaluated under seven stress conditions using principle component analysis of gene expression. the results showed that growth of the pathogen was reduced by h₂o₂ and paraquat-induc ... | 2015 | 26378528 |
| sugarcane transcriptome analysis in response to infection caused by acidovorax avenae subsp. avenae. | sugarcane is an important tropical crop mainly cultivated to produce ethanol and sugar. crop productivity is negatively affected by acidovorax avenae subsp avenae (aaa), which causes the red stripe disease. little is known about the molecular mechanisms triggered in response to the infection. we have investigated the molecular mechanism activated in sugarcane using a rna-seq approach. we have produced a de novo transcriptome assembly (tr7) from sugarcane rna-seq libraries submitted to drought an ... | 2016 | 27936012 |
| avrrxo1 is a bifunctional type iii secreted effector and toxin-antitoxin system component with homologs in diverse environmental contexts. | toxin-antitoxin (ta) systems are ubiquitous bacterial systems that may function in genome maintenance and metabolic stress management, but are also thought to play a role in virulence by helping pathogens survive stress. we previously demonstrated that the xanthomonas oryzae pv. oryzicola protein avrrxo1 is a type iii-secreted virulence factor that has structural similarities to the zeta family of ta toxins, and is toxic to plants and bacteria in the absence of its predicted chaperone arc1. in t ... | 2016 | 27391081 |
| regulatory role of tetr gene in a novel gene cluster of acidovorax avenae subsp. avenae rs-1 under oxidative stress. | acidovorax avenae subsp. avenae is the causal agent of bacterial brown stripe disease in rice. in this study, we characterized a novel horizontal transfer of a gene cluster, including tetr, on the chromosome of a. avenae subsp. avenae rs-1 by genome-wide analysis. tetr acted as a repressor in this gene cluster and the oxidative stress resistance was enhanced in tetr-deletion mutant strain. electrophoretic mobility shift assay demonstrated that tetr regulator bound directly to the promoter of thi ... | 2014 | 25374564 |
| inhibitory effect of camptothecin against rice bacterial brown stripe pathogen acidovorax avenae subsp. avenae rs-2. | camptothecin (cpt) has anticancer, antiviral, and antifungal properties. however, there is a dearth of information about antibacterial activity of cpt. therefore, in this study, we investigated the inhibitory effect of cpt on acidovorax avenae subsp. avenae strain rs-2, the pathogen of rice bacterial brown stripe, by measuring cell growth, dna damage, cell membrane integrity, the expression of secretion systems, and topoisomerase-related genes, as well as the secretion of effector protein hcp. r ... | 2016 | 27472315 |
| non-destructive evaluation of bacteria-infected watermelon seeds using visible/near-infrared hyperspectral imaging. | there is a need to minimize economic damage by sorting infected seeds from healthy seeds before seeding. however, current methods of detecting infected seeds, such as seedling grow-out, enzyme-linked immunosorbent assays, the polymerase chain reaction (pcr) and the real-time pcr have a critical drawbacks in that they are time-consuming, labor-intensive and destructive procedures. the present study aimed to evaluate the potential of visible/near-infrared (vis/nir) hyperspectral imaging system for ... | 2017 | 27264863 |
| relationship of soil properties and sugarcane yields to red stripe in louisiana. | symptoms of red stripe disease caused by acidovorax avenae subsp. avenae in louisiana between 1985 and 2010 were limited to the leaf stripe form, which caused no apparent yield loss. during 2010, the more severe top rot form was observed, and a study was initiated to investigate the distribution of red stripe in the field and determine its effects on cane and sugar yields. soil properties data, red stripe incidence, and sugarcane yields were all highly variable and were not randomly distributed ... | 2016 | 27003508 |
| erratum for fontana et al., genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | | 2016 | 26988059 |
| new insights into virulence mechanisms of rice pathogen acidovorax avenae subsp. avenae strain rs-1 following exposure to ß-lactam antibiotics. | recent research has shown that pathogen virulence can be altered by exposure to antibiotics, even when the growth rate is unaffected. investigating this phenomenon provides new insights into understanding the virulence mechanisms of bacterial pathogens. this study investigates the phenotypic and transcriptomic responses of the rice pathogenic bacterium acidovorax avenae subsp. avenae (aaa) strain rs-1 to ß-lactam antibiotics especially ampicillin (amp). our results indicate that exposure to amp ... | 2016 | 26915352 |
| multiplex pcr assay for simultaneous detection of six major bacterial pathogens of rice. | the aim of this study was to develop a multiplex pcr (mpcr) assay for rapid, sensitive and simultaneous detection of six important rice pathogens: xanthomonas oryzae pv. oryzae, x. oryzae pv. oryzicola, pseudomonas fuscovaginae, burkholderia glumae, burkholderia gladioli and acidovorax avenae subsp. avenae. | 2016 | 26864896 |
| genome sequence of acidovorax avenae strain t10_61 associated with sugarcane red stripe in argentina. | red stripe of sugarcane in argentina is a bacterial disease caused by acidovorax avenae. the genome sequence from the first isolate of this bacterium in argentina is presented here. the draft genome of the a. avenae t10_61 strain contains 5,646,552 bp and has a g+c content of 68.6 mol%. | 2016 | 26847889 |
| iren, a novel ef-hand motif-containing nuclease, functions in the degradation of nuclear dna during the hypersensitive response cell death in rice. | the hypersensitive response (hr), a type of programmed cell death that is accompanied by dna degradation and loss of plasma membrane integrity, is a common feature of plant immune responses. we previously reported that transcription of iren which encodes a novel ef-hand containing plant nuclease is controlled by osnac4, a key positive regulator of hr cell death. transient overexpression of iren in rice protoplasts also led to rapid dna fragmentation, while suppression of iren using rna interfere ... | 2016 | 26766411 |
| membrane protein profiling of acidovorax avenae subsp. avenae under various growth conditions. | membrane proteins (mps) of plant pathogenic bacteria have been reported to be able to regulate many essential cellular processes associated with plant disease. the aim of the current study was to examine and compare the expression of mps of the rice bacterial pathogen acidovorax avenae subsp. avenae strain rs-1 under luria-bertani (lb) medium, m9 medium, in vivo rice plant conditions and leaf extract (le) medium mimicking in vivo plant condition. proteomic analysis identified 95, 72, 75, and 87 ... | 2015 | 25763989 |
| cd2-1, the c-terminal region of flagellin, modulates the induction of immune responses in rice. | flagellin from the rice avirulent n1141 strain of acidovorax avenae functions as a pathogen-associated molecular pattern (pamp) and induces pamp-triggered immunity (pti) in rice. to study the recognition mechanism of flagellin in rice, we attempted to define one or more regions of the flagellin protein required to activate the pti response. based on domain classification, we produced four fragments of n1141 flagellin: n-terminal d0, d1. and d2 domains (nd0-2), n-terminal d2, d3, and c-terminal d ... | 2015 | 25625819 |
| rice osfls2-mediated perception of bacterial flagellins is evaded by xanthomonas oryzae pvs. oryzae and oryzicola. | bacterial flagellins are often recognized by the receptor kinase flagellin sensitive2 (fls2) and activate mamp-triggered immunity in dicotyledonous plants. however, the capacity of monocotyledonous rice to recognize flagellins of key rice pathogens and its biological relevance remain poorly understood. we demonstrate that ectopically expressed osfls2 in arabidopsis senses the eliciting flg22 peptide and in vitro purified acidovorax avenae (aa) flagellin in an expression level-dependent manner, b ... | 2015 | 25617720 |
| two types of genetic carrier, the incp genomic island and the novel incp-1β plasmid, for the aac(2')-iia gene that confers kasugamycin resistance in acidovorax avenae ssp. avenae. | a unique aminoglycoside antibiotic, kasugamycin (ksm), has been used to control many plant bacterial and fungal diseases in several countries. the emergence of ksm-resistant acidovorax avenae ssp. avenae and burkholderia glumae, which cause rice bacterial brown stripe and rice bacterial grain and seedling rot, respectively, is a serious threat for the effective control of these diseases. previously, we have identified the aac(2')-iia gene, encoding a ksm 2'-n-acetyltransferase, from both ksm-res ... | 2015 | 25131295 |
| inhibitory effect and mode of action of chitosan solution against rice bacterial brown stripe pathogen acidovorax avenae subsp. avenae rs-1. | inhibitory effect and mode of action of chitosan solution against rice bacterial brown stripe pathogen acidovorax avenae subsp. avenae (aaa) strain rs-1 was examined in this study. result from this study indicated that chitosan solutions at 0.10, 0.20, and 0.40mg/ml inhibited the in vitro growth of aaa strain rs-1, and in general the inhibitory efficiency increased with the increase of both chitosan concentration and the incubation time. antibacterial activity of chitosan in this study may be ma ... | 2014 | 24785387 |
| two distinct ef-tu epitopes induce immune responses in rice and arabidopsis. | plants sense potential pathogens by recognizing conserved pathogen-associated molecular patterns (pamps) that cause pamp-triggered immunity (pti). we previously reported that rice recognizes flagellin from the rice-incompatible n1141 strain of acidovorax avenae and subsequently induces immune responses. cell extracts isolated from flagellin-deficient n1141 (δfla1141) still induced pti responses, suggesting that δfla1141 possesses an additional pamp distinct from flagellin. here, we show that elo ... | 2014 | 24200076 |
| description of acidovorax wautersii sp. nov. to accommodate clinical isolates and an environmental isolate, most closely related to acidovorax avenae. | three gram-negative strains, nf 1078(t), nf 1598 and nf 1715, were isolated from clinical (two) and environmental (one) samples, respectively. sequence analysis of the 16s rrna genes revealed similarity of 100% among the three strains and next highest similarity to the type strain of acidovorax avenae (98.16%). the three strains were able to acidify lactose and rhamnose on low peptone phenol red agar and to alkalinize citrate on simmons' agar and were negative for nitrate reduction. the dna g+c ... | 2013 | 23148096 |
| role of individual positive charges in the membrane orientation and activity of transporters of the small multidrug resistance family. | the effect of individual positively charged residues on the orientation in the membrane was analyzed in three dual-topology transporters of the small multidrug resistance (smr) family: aave4701aave of acidovorax avenae, emreecol of escherichia coli, and rrua0272rrub of rhodospirillum rubrum. it is shown that (i) individual positive charges have different impacts on the orientation, (ii) positive charges that are conserved in the three different proteins do not have the same impact on the orienta ... | 2012 | 23043311 |
| genetic organization of the hrp gene cluster in acidovorax avenae strain n1141 and a novel effector protein that elicits immune responses in rice (oryza sativa l.). | the immune system of plants consists of two main arms, pathogen-associated molecular pattern (pamp)-triggered immunity (pti) and effector-triggered immunity (eti). the multiple effectors that trigger eti are translocated into plant cells by the type iii secretion system (t3ss) of pathogenic bacteria. the rice-avirulent n1141 strain of acidovorax avenae causes eti in rice, including hypersensitive response (hr) cell death. sequence analysis indicated that the n1141 genome contains the hrp gene cl ... | 2012 | 22232252 |