| radiocopper in l-tryptophan 2,3-dioxygenase isolated from pseudomonas acidovorans grown in the presence of 64cu (ii). | l-tryptophan 2,3-dioxygenase (ec 1.13.11.11), isolated from l-tryptophan-induced pseudomonas acidovorans, atcc 11299b, which has been grown in a medium containing 64cu(no3)2, has been shown to contain radiocopper. at several stages of purification of the enzyme samples were taken, and these were subjected to disc acrylamide gel electrophoresis in the presence of 10 mm l-tryptophan. after electrophoresis the position of the yellow heme band, corresponding to tryptophan oxygenase, was visually loc ... | 1975 | 49351 |
| humic-like substances of bacterial origin. i. some aspects of the formation and nature of humic-like substances produced by pseudomonas. | two bacterial strains pseudomonas acidovorans no 26 and pseudomonas sp. no 4 grown in conn and yeast extract-glucose media, or in the media enriched with tyrosine, were found to produce dark brown pigment. it was shown that in the bacterial cultures numerous phenolic and quinone-type compounds were formed and transformed to humic-like polymers. formation of humic-like substances started in the bacterial cells and was accompanied by the presence of phenyloxidases in the bacterial cultures. the ba ... | 1977 | 75666 |
| humic-like substances of bacterial origin. ii. fractionation of the bacterial humic-like substances by gel filtration on sephadex gels. | humic-like substances obtained from cells of pseudomonas acidovorans were separated on sephadex g-25 into two groups of substances of different molecular weight. the substances of the molecular weight greater than 5000 were successively separated on sephadex gels g-50, g-75, g-100. five fractions of different molecular weight were obtained, the percentage of which varied depending on the media used and time of incubation of the bacteria. most (38%--46%) of the compounds contained in the bacteri ... | 1977 | 75667 |
| humic-like substances of bacterial origin. iii. production of humic-like substances by pseudomonas acidovorans in media containing certain benzene derivatives. | | 1977 | 75668 |
| fructose metabolism in four pseudomonas species. | 1. atp-dependent phosphorylation of fructose could not be detected in extracts of fructose-grown cells of pseudomonas extorquens strain 16, pseudomonas 3a2, pseudomonas acidovorans and pseudomonas fluorescens. instead, phosphorylation of fructose to fructose-1-phosphate was found to occur when cell-free extracts were incubated with fructose and phosphoenolpyruvate. such an activity could not be detected in cell-free extracts of succinate-grown cells. 2. high levels of 1-phosphofructokinase were ... | 1977 | 143919 |
| dissimilation of the lignin model compound veratrylglycerol-beta-(o-methoxyphenyl) ether by pseudomonas acidovorans: initial transformations. | the initial transformadomonas acidovorans are demethylation of the 4-methoxyl group of the 3,4-dimethoxyphenyl portion of the molecule, and a nad+-linked dehydrogenation of the benzyl alcohol group. based on these findings and those described before, an overall degradation scheme is postulated. | 1975 | 164269 |
| tryptophan 2,3-dioxygenase: a review of the roles of the heme and copper cofactors in catalysis. | l-tryptophan, 2,3-dioxygenase (ec 1.13.11.11) has been purified to homogenity from l-tryptophan induced pseudomonas acidovorans (atcc 11299b) and from l-tryptophan and cortisone induced rat liver. the enzyme from both sources is composed of four subunits and contains two g-atoms copper and two moles heme per mole tetramer. the proteins from the two sources are not identical. three oxidation states of tryptophan oxygenase have been isolated: (1) fully oxidized, [cu(ii)]2[ferriheme]2; (2) half red ... | 1975 | 178384 |
| metabolism of carbohydrate derivatives by pseudomonas acidovorans. | wild-type pseudomonas acidovorans strain a1 was unable to grow on glycerol or glucose as sole source of carbon and energy although it grew well on gluconate. spontaneous glycerol-positive mutants, which apparently had become permeable to glycerol, were readily isolated, but glucose-positive mutants did not occur. p. acidovorans lacked glucose dehydrogenase and glucokinase, which were sufficient to account for its inability to grow on glucose. gluconate was degraded exclusively via a noncoordinat ... | 1979 | 220214 |
| metabolic function and properties of 4-hydroxyphenylacetic acid 1-hydroxylase from pseudomonas acidovorans. | the enzyme 4-hydroxyphenylacetate, nad(p)h:oxygen oxidoreductase (1-hydroxylating) (ec 1.14.13 ...; 4-hydroxyphenylacetate 1-monooxygenase; referred to here as 4-hpa 1-hydroxylase) was induced in pseudomonas acidovorans when 4-hydroxyphenylacetate (4-pha) was utilized as carbon source for growth; homogentisate and maleylacetoacetate were intermediates in the degradation of 4-hpa. a preparation of the hydroxylase that was free from homogentisate dioxygenase and could be stored at 4 c in the prese ... | 1975 | 234937 |
| characterization of an inducible amidase from pseudomonas acidovorans ae1. | the main molecular and catalytic properties of an acetanilide-hydrolyzing enzyme from pseudomonas acidovorans ae 1, purified to a homogeneous state, were investigated. the molecular weight was 57 500 as determined by gel filtration and 55 300 as computed from the amino acid composition. by polyacrylamide gel electrophoresis in dodecylsulfate a polypeptide chain weight of 56 700 was obtained. based on the reaction of the highly purufied enzyme with diethyl-4-nitrophenyl phosphate an equivalent we ... | 1975 | 237757 |
| structural, kinetic, and renaturation properties of an induced hydroxypyruvate reductase from pseudomonas acidovorans. | a hydroxypyruvate reductase has been induced in pseudomonas acidovorans by growth on glyoxylate. the enzyme has been purified to homogeneity as assessed by the criteria of analytical ultracentrifugation and analytical disc gel electrophoresis. it has a molecular weight of approximately 85,000 and is composed of two identical subunits. the subunits are not interconnected by disulfide bonds although the enzyme has 4 mol of half-cystine per mol of enzyme. the enzyme catalyzes the reversible convers ... | 1975 | 238981 |
| ph dependence of the cooperative interactions and conformation of tryptophan oxygenase. | allosteric interactions in the cupro-heme enzyme tryptophan oxygenase (ec 1.13.11.11) of pseudomonas acidovorans are shown to be ph-dependent. increasing the assay ph from 6.0 to 8.0 progressively desensitizes the enzyme from both homotropic and heterotropic ligand interactions. this ph-dependent reversible transition has a pk of 6.2. hill coefficients for the substrate l-tryptophan of 2.0 and 1.4 were measured at ph 6.0 and ph 7.0, respectively. in attempting to identify the enzymatic residue ( ... | 1975 | 239940 |
| amber suppressor mutations in pseudomonas acidovorans. | almost 50% of the clones of pseudomonas acidovorans(plm2) selected for resistance to tetracycline supported the growth of an amber mutant of bacteriophage prd1. | 1979 | 370094 |
| incompatibility group p-1 bla+ plasmids do not increase penicillin resistance of pseudomonas acidovorans. | incompatibility group p-1 plasmids with the bla+ genotype were transferred from various escherichia coli strains to pseudomonas acidovorans strain 29. when resistance to ampicillin was used as the criterion, none of these plasmids appeared able to express their bla+ phenotype in this host. when the plasmids were subsequently transferred back from these ampicillin-sensitive p. acdiovorans transcipients to e. coli strains, it was found that the bla+ phenotype was again expressed. although beta-lac ... | 1979 | 383693 |
| coexistence of different pathways in the metabolism of n-propylbenzene by pseudomonas sp. | pseudomonas desmolytica s449b1 and pseudomonas convexa s107b1 grown on n-propylbenzene oxidized n-propylbenzene to beta-phenylpropionic acid and benzoic acid by initial oxidation of the n-propyl side chain and the following beta-oxidation, respectively. the same strains also oxidized n-propylbenzene to 3-n-propylcatechol by initial oxidation of positions 2 and 3 of the aromatic nucleus. a ring fission product, 2-hydroxy-6-oxononanoic acid, was also isolated from the culture broth. together with ... | 1979 | 543699 |
| the active site of an inducible arylacylamidase from pseudomonas acidovorans. | though very effectively ibhibited by both sh-group directed reagents and inhibitors typical for serine hydrolases, the inducible arylacylamidase from pseudomonas acidovarans is a serine enzyme rather than a sulfhydryl enzyme. the amino acid sequence around the reactive serine residue is gly-ser-ile. the sequence of a larger peptide from the active site is described. | 1978 | 631987 |
| newly discovered polyamine, 2-hydroxyspermidine, in pseudomonas acidovorans. | a previously unknown hydroxylated polyamine has been recovered from pseudomonas acidovorans 29. it has been identified as 2-hydroxyspermidine, n4-(3-aminopropyl)-1,4-diaminobutane-2-ol, by its chromatographic behavior, electrophoretic mobility, and reaction with metaperiodate. it can be synthesized enzymatically from 2-hydroxyputrescine by cell-free preparations from escherichia coli or p. acidovorans 29 which contain propylamine transferase. it is interesting to note that the naturally occurrin ... | 1978 | 690077 |
| susceptibility of nonfermentative gram-negative bacilli to tobramycin. | there has been increasing interest in the pathogenic role of nonfermentative gram-negative bacilli in human infections. except for pseudomonas aeruginosa, the susceptibility pattern of these organisms to tobramycin has not been evaluated thoroughly. the activity of tobramycin, as compared with that of gentamicin, was tested by the serial broth dilution technique against 178 isolates of nonfermentative gram-negative bacilli obtained from various sources. p. aeruginosa, pseudomonas stutzeri, acine ... | 1976 | 823278 |
| purification and properties of l-glutaminase-l-asparaginase from pseudomonas acidovorans. | an enzyme that catalyzes the hydrolysis of both glutamine and asparagine has been purified to homogeneity from extracts of pseudomonas acidovorans. the enzyme having a ratio of glutaminase to asparaginase of 1.45:1.0 can be purified by a relatively simple procedure and is stable upon storage. the glutaminase-asparaginase has a relatively high affinity for l-asparagine (km=1.5 x 10(-5) m) and l-glutamine (km=2.2 x 10(-5) m) and has a molecular weight of approximately 156,000 the subunit molecular ... | 1977 | 845119 |
| polyamines in bacteriophage phi w-14 and in phi w-14-infected pseudomonas acidovorans. | bacteriophage phi w-14 is sensitive to osmotic shock. it contain sufficient free putrescine, 2-hydroxyputrescine and spermidine to neutralize about 15% of the dna phosphates. the alpha-putrescinylthymine residues of the dna could neutralize a further 25% of the phosphates. label is transferred from ornithine to the alpha-putrescinyl residues of phi w-14 dna. the rates of polyamine synthesis in pseudomonas acidovorans are increased by phi w-14 infection. | 1976 | 978181 |
| isolation of an inducible amidase from pseudomonas acidovorans ae1. | a bacterial strain, aei, which hydrolysed acetanilide, was isolated from soil and identified as pseudomonas acidovorans. numerous amides, esters and enzyme inhibitors were tested as amidase inducers. phenacetin was chosen as inducer for the large scale cultivation of these organisms because it was less toxic to the bacteria than acetanilide. the induction increased the enzymic activity 250-fold. in comparison, the type culture strain of p. acidovorans, attcci5668, had no amidase activity which c ... | 1975 | 1141856 |
| studies on the subunit structure of 4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating) from pseudomonas acidovorans. | 1. homogeneous preparations of d-4-deoxy-5-oxoglutarate hydro lyase (decarboxylating)(ec4.2.1.41) were analysed in the ultracentrifuge by the high-speed sedimentation-equilibrium method of yphantis (1964). the molecular weight in 0.1 m-potassium phosphate buffer, ph 7.2, in 6m-guanidine hydrochloride and in 0.1 m-beta-mercaptoethanol in 6m-guanidine hydrochloride was 113,000, 56,000 and 30,400 respectively. polyacrylamidegel electrophoresis in the presence of sodium dodecyl sulphate indicated a ... | 1975 | 1156361 |
| purification and properties of xanthine dehydrogenase from pseudomonas acidovorans. | xanthine dehydrogenase (ec 1.2.1.37) from pseudomonas acidovorans has been purified to near homogeneity (approx. 65-fold). the enzyme has a molecular weight of about 275 000. electrophoresis in gels containing sodium dodecyl sulphate showed the presence of two types of subunit with molecular weights of about 81 000 and 63 000. thus the intact molecule probably contains two of each type of subunit. xanthine and hypoxanthine are good substrates, and nad+ is an effective electron acceptor. with xan ... | 1975 | 1191668 |
| alternative routes of aromatic catabolism in pseudomonas acidovorans and pseudomonas putida: gallic acid as a substrate and inhibitor of dioxygenases. | when 3,4-dihydroxyphenylacetic acid (homoprotocatechuic acid) was added to pseudomonase acidovorans growing at the expense of succinate, enzymes required for degrading homoprotocatechuate to pyruvate and succinate semialdehyde were strongly induced. these enzymes were effectively absent from cell extracts of the organism grown with 4-hydroxyphenylacetic acid, and this substrate was metabolized by the catabolic enzymes of the homogentisate pathway. two separate ring-fission dioxygenases for 3,4,5 ... | 1975 | 1194238 |
| isolation of regulatory mutants of pseudomonas acidovorans by use of amino acid analogs. | mutants resistance to various combinations of threonine, lysine and/or their analogs were obtained and characterized in pseudomonas acidovorans. in particular, mutants resistant to aminoethylcysteine had a dihydrodipicolinate synthetase insensitive to lysine inhibition whereas mutants resistant to threonine plus a low concentration of aminoethylcysteine had a feedback-insensitive aspartokinase. | 1976 | 1259520 |
| identification of xenobiotic-degrading isolates from the beta subclass of the proteobacteria by a polyphasic approach including 16s rrna partial sequencing. | nineteen gram-negative, aerobic, biodegradative isolates were identified by using a polyphasic taxonomic approach. the presence of the specific polyamine 2-hydroxyputrescine and the presence of a ubiquinone with eight isoprenoid units in the side chain (ubiquinone q-8) allowed allocation of these organisms to the beta subclass of the proteobacteria. on the basis of the results of additional characterization experiments (i.e., api 20ne tests, determinations of soluble protein patterns, and dna-dn ... | 1992 | 1371062 |
| topology of the anion-selective porin omp32 from comamonas acidovorans. | limited proteolysis experiments were performed with outer membranes from comamonas acidovorans to probe the topology of its major protein component, the anion-selective porin omp32. proteinase k treatment above a critical temperature of 42 degrees c cleaved the surface-exposed regions of the porin, yielding membrane-embedded fragments which were separated by sds polyacrylamide gel electrophoresis or reversed phase chromatography. the identification of the proteinase k-sensitive sites was perform ... | 1992 | 1373289 |
| characterization of isofunctional ring-cleaving enzymes in aniline and 3-chloroaniline degradation by pseudomonas acidovorans ca28. | during degradation of aniline and 3-chloroaniline, respectively, by pseudomonas acidovorans ca28, selective induction of two catechol 1,2-dioxygenases (c12o) was observed. c12o i activity was the sole ring-cleaving enzyme detectable in cell-free extracts after growth on aniline, while c12o ii was exclusively found after growth on 3-chloroaniline. both enzymes were clearly differentiated by their elution behaviour on deae-cellulose and their substrate specificities. for c12o i high activity was d ... | 1992 | 1427016 |
| novel degradative pathway of 4-nitrobenzoate in comamonas acidovorans nba-10. | a comamonas acidovorans strain, designated nba-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. when grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. in cell extracts with nadph present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-ni ... | 1992 | 1527502 |
| chemical and cultural characterization of cdc group wo-1, a weakly oxidative gram-negative group of organisms isolated from clinical sources. | ninety-six strains of weakly oxidative gram-negative rods isolated primarily from clinical specimens form a distinct group that has been designated centers for disease control (cdc) group wo-1 (wo stands for weak oxidizer). the phenotypic characteristics of cdc group wo-1 were most similar to those of comamonas acidovorans, pseudomonas mallei, and cdc pink coccoid group iii. the wo-1 group can be differentiated from c. acidovorans by the oxidation of glucose (often weak and sometimes delayed), m ... | 1992 | 1537895 |
| two-dimensional crystallization of a bacterial surface protein on lipid vesicles under controlled conditions. | the solubilized surface protein of the gram-negative bacterium comamonas acidovorans was reconstituted on lipid vesicles by means of controlled dialysis. to this end, a multichamber dialysis apparatus was built which allows one to control the temperature and the dialysis rate, to apply various temperatures or buffer systems and sample conditions in a single experiment, and to monitor the turbidity of the sample by means of light scattering. the reconstitution conditions were optimized such that ... | 1992 | 1540688 |
| pseudomonas infections in patients with aids and aids-related complex. | we identified and reviewed retrospectively all the cases of infection by pseudomonas and related genera in patients with aids and aids-related complex (arc) who were hospitalized at our institution over a 36-month period. we recorded 48 episodes of infection in 34 of 355 patients with aids, and in two of 73 patients with arc: 25 pneumonias (9 community-acquired and 16 of nosocomial origin). 20 urinary tract infections, two soft tissue infections and one sepsis. in 14 of 16 patients with nosocomi ... | 1992 | 1588272 |
| inducible beta-lactamases in clinical isolates of non-aeruginosa pseudomonas. | the incidence of antimicrobial resistance and expression of imipenem-inducible beta-lactamase were examined in 22 strains of non-aeruginosa pseudomonas isolated from clinical specimens. the percentage of strains resistant to form one to eight antibiotics was 45. the most active antibiotics against all strains were norfloxacin, ciprofloxacin and imipenem. eighteen out of the 22 strains were positive for beta-lactamase in a spectrophotometric assay using nitrocefin as substrate. a low inducible be ... | 1992 | 1610551 |
| outer membranes of gram-negative bacteria are permeable to steroid probes. | the permeability of bacterial outer membranes was assayed by coupling the influx of highly hydrophobic probes, 3-oxosteroids, with their subsequent oxidation catalysed by 3-oxosteroid delta 1-dehydrogenase, expressed from a gene cloned from pseudomonas testosteroni. in salmonella typhimurium producing wild-type lipopolysaccharide, the permeability coefficients for uncharged steroids were 0.45 to 1 x 10(-5) cm s-1, and the diffusion appeared to occur mainly through the lipid bilayer domains of th ... | 1992 | 1640833 |
| the three-dimensional structure of the regular surface protein of comamonas acidovorans derived from native outer membranes and reconstituted two-dimensional crystals. | the three-dimensional structure of the regular surface protein (p4 symmetry, lattice constant a = b = 10.5 nm) of comamonas acidovorans has been determined to a resolution of about 1.5 nm by means of electron microscopy and image processing. three-dimensional reconstructions were performed using native outer membranes and artificial two-dimensional crystals of the surface protein, which was selectively solubilized by deoxycholate and recrystallized on carbon films. the two-fold symmetric morphol ... | 1991 | 1719335 |
| nucleotide and derived amino acid sequences of the major porin of comamonas acidovorans and comparison of porin primary structures. | the dna sequence of the gene which codes for the major outer membrane porin (omp32) of comamonas acidovorans has been determined. the structural gene encodes a precursor consisting of 351 amino acid residues with a signal peptide of 19 amino acid residues. comparisons with amino acid sequences of outer membrane proteins and porins from several other members of the class proteobacteria and of the chlamydia trachomatis porin and the neurospora crassa mitochondrial porin revealed a motif of eight r ... | 1991 | 1848840 |
| ocular infections associated with comamonas acidovorans. | comamonas acidovorans (pseudomonas acidovorans) is a ubiquitous gram-negative rod. although generally considered nonpathogenic, we found c. acidovorans to be associated with six cases of ocular infections. the organism was the only isolate in three cases, whereas an association of other organisms was present in three cases. the multiple resistance patterns of these strains to antibiotic susceptibility testing emphasizes the need for culturing ocular infections. we recommend the identification an ... | 1991 | 1882921 |
| electron microscopy and image analysis reveal common principles of organization in two large protein complexes: groel-type proteins and proteasomes. | in an attempt to settle the question of whether the multicatalytic proteinase or proteasome exist in all three kingdoms of life--eukaryotes, archaebacteria, and eubacteria--we have undertaken a search for them in the eubacterium comamonas acidovorans. we have, in fact, isolated and purified a cylinder-shaped particle. however, according to various structural and biochemical criteria this turned out to be more reminiscent of the groel protein from escherichia coli and its homologs than to proteas ... | 1990 | 1979749 |
| gentisate 1,2-dioxygenase from pseudomonas. purification, characterization, and comparison of the enzymes from pseudomonas testosteroni and pseudomonas acidovorans. | the 3-hydroxybenzoate inducible gentisate 1,2-dioxygenases have been purified to homogeneity from p. acidovorans and p. testosteroni, the two divergent species of the acidovorans group of pseudomonas. both enzymes exhibit a 40-fold higher specific activity than previous preparations and have an (alpha fe)4 quaternary structure (holoenzyme mr = 164,000 and 158,000, respectively). the enzymes have different amino terminal sequences, amino acid contents, and isoelectric points. each enzyme contains ... | 1990 | 2156846 |
| gentisate 1,2-dioxygenase from pseudomonas. substrate coordination to active site fe2+ and mechanism of turnover. | gentisate 1,2-dioxygenase catalyzes the oxygenolytic ring cleavage of gentisate (2,5-dihydroxybenzoate) between carbons 1 and 2 to form maleylpyruvate. the essential active site fe2+ of the enzyme binds no to yield an epr-active (s = 3/2) complex. hyperfine broadening from 17o (i = 5/2) is observed in the spectrum of the enzyme-nitrosyl complex prepared in 17o-enriched water, demonstrating that water is an iron ligand. association of gentisate with the enzyme-nitrosyl complex causes the broadeni ... | 1990 | 2266121 |
| gentisate 1,2-dioxygenase from pseudomonas acidovorans. | | 1990 | 2280694 |
| endocarditis associated with comamonas acidovorans. | a case of endocarditis caused by comamonas acidovorans (pseudomonas acidovorans) in a 42-year-old intravenous-drug abuser is described. this article appears to be the first detailed report of the isolation of this organism from a systemic clinical infection and its identification as a pathogen. | 1990 | 2298872 |
| isolation and characterization of monoclonal antibodies against alkaline phosphatase of pseudomonas aeruginosa. | monoclonal antibodies against the alkaline phosphatase of pseudomonas aeruginosa were produced from spleen cells of balb/c mice primed with purified alkaline phosphatase of p. aeruginosa atcc 10145 and sp20/ag-14 myeloma cells. the eight stable clones established produced antibodies that reacted by enzyme-linked immunosorbent and indirect immunofluorescence assays with all bacterial strains of p. aeruginosa, including the 17 serotypes and two nontypable strains. three of the clones cross-reacted ... | 1989 | 2501343 |
| [a sarcoma-static new species of pseudomonas, pseudomonas jinanensis sp. nov]. | a strain of gram negative bacteria was isolated from the surface soil of wuying hill at jinan, shandong province with gause's medium in 1973. it is a strain of antagonistic bacteria for hysterocervicoma, hepatoma and melanoma of mice screened from 2100 strains of bacteria. it is also antagonistic to staphylococcus aureus, bacillus subtilis and micrococcus. it is a gram negative bacterium with lophotrichous polar flagella. straight rods in shape or with a little slightly curved rods, 0.5-0.6 x 1- ... | 1989 | 2781786 |
| cosmid cloning of five zymomonas trp genes by complementation of escherichia coli and pseudomonas putida trp mutants. | a library of zymomonas mobilis genomic dna was constructed in the broad-host-range cosmid plafr1. the library was mobilized into a variety of escherichia coli and pseudomonas putida trp mutants by using the helper plasmid prk2013. five z. mobilis trp genes were identified by the ability to complement the trp mutants. the trpf, trpb, and trpa genes were on one cosmid, while the trpd and trpc genes were on two separate cosmids. the organization of the z. mobilis trp genes seems to be similar to th ... | 1988 | 2838460 |
| phenylalanine hydroxylase and isozymes of 3-deoxy-d-arabino-heptulosonate 7-phosphate synthase in relationship to the phylogenetic position of pseudomonas acidovorans (ps. sp. atcc 11299a). | the evolution of aromatic amino acid biosynthesis and its regulation is under study in a large assemblage of prokaryotes (superfamily a) whose phylogenetic arrangement has been constructed on the criterion of oligonucleotide cataloging. one section of this superfamily consists of a well defined (rrna homology) cluster denoted as group iii pseudomonads. pseudomonas acidovorans atcc 11299a, a group iii member, was chosen for indepth studies of 3-deoxy-d-arabino-heptulosonate 7-phosphate (dahp) syn ... | 1985 | 2859843 |
| the cefoperazone-sulbactam combination. in vitro qualities including beta-lactamase stability, antimicrobial activity, and interpretive criteria for disk diffusion tests. | three concentrations of the penicillanic acid sulfone, sulbactam were tested in combination with cefoperazone against 632 recent clinical bacterial isolates. cefoperazone was effective alone (less than or equal to 16 micrograms/ml) against 95% of enterobacteriaceae and combined with 4 micrograms/ml sulbactam inhibited 99.5% of strains. this coverage of enteric bacilli was superior to timentin (99.1%), ceftazidime (98.2%), and tobramycin (90.9%). the minimum inhibitory concentrations (mics) of ce ... | 1985 | 2994461 |
| in vitro antimicrobial activity of cefoperazone-sulbactam combinations against 554 clinical isolates including a review and beta-lactamase studies. | cefoperazone was tested against 554 clinical isolates alone and with sulbactam in three combinations. the addition of sulbactam in low concentrations (less than or equal to 4 micrograms/ml) improved the spectrum of cefoperazone principally against gram-negative bacilli such as acinetobacter species, some pseudomonas species, and beta-lactamase-positive enterobacteriaceae. nearly all of the spectrum increase was achieved at a sulbactam level of less than or equal to 2 micrograms/ml. sulbactam was ... | 1985 | 2998694 |
| biochemical and genetic studies of bacteria metabolizing lignin-related compounds. | the ability of bacterial strains to metabolize lignin model compounds was studied. strains examined were non-filamentous bacterial isolates obtained from decaying wood and the actinomycete streptomyces viridosporus t7a. model compounds included dimers containing either the beta-1 (1,2-diarylethane) or the beta-o-4 (arylglycerol-beta-aryl ether) type of linkage. pseudomonas fluorescens biovar i a1 proliferated on anisoin (4,4'-dimethoxybenzoin) accumulating anisic acid temporarily. cleavage at th ... | 1988 | 3154864 |
| cefetamet pivoxil: bacteriostatic and bactericidal activity of the free acid against 355 gram-negative rods. | the in vitro activity of the free acid of cefetamet pivoxil (ro 15-8075) was tested against 355 clinical isolates, namely enteropathogenic bacteria, glucose non-fermentative gram-negative rods (excluding pseudomonas aeruginosa) and legionella pneumophila. ceftriaxone was included in the study as reference compound. although the free acid of the orally active cephalosporin was generally weaker than ceftriaxone, it inhibited 88.2% and 94.5% of enterobacteriaceae and vibrionaceae at a concentration ... | 1988 | 3403040 |
| infrared spectra of carbon monoxide complexes of indoleamine 2,3-dioxygenase and l-tryptophan 2,3-dioxygenases. effects of substrates on the co-stretching frequencies. | carbonmonoxy indoleamine 2,3-dioxygenase from rabbit small intestine exhibited two co stretch bands at 1953 and 1933 cm-1 with half-band widths (delta v 1/2) of both approximately 15 cm-1. upon addition of an excess amount of l-tryptophan, the substrate, the spectrum changed into that with an intense single band at 1902 cm-1 with the delta v 1/2 of 15 cm-1. carbonmonoxy l-tryptophan 2,3-dioxygenase of pseudomonas acidovorans in the absence of l-tryptophan showed a fused co stretch band which con ... | 1985 | 3871436 |
| effects of dissolved organic carbon and second substrates on the biodegradation of organic compounds at low concentrations. | pseudomonas acidovorans and pseudomonas sp. strain anl but not salmonella typhimurium grew in an inorganic salts solution. the growth of p. acidovorans in this solution was not enhanced by the addition of 2.0 micrograms of phenol per liter, but the phenol was mineralized. mineralization of 2.0 micrograms of phenol per liter by p. acidovorans was delayed 16 h by 70 micrograms of acetate per liter, and the delay was lengthened by increasing acetate concentrations, whereas phenol and acetate were u ... | 1985 | 3890738 |
| models for the kinetics of biodegradation of organic compounds not supporting growth. | we developed 12 models of kinetics to describe the metabolism of organic substrates that are not supporting bacterial growth. these models can be used to describe the biodegradation of organic compounds that are not supporting growth when the responsible populations are growing logistically, logarithmically, or linearly or are not increasing in numbers. nonlinear regression analysis was used to fit patterns of mineralization by two bacteria to these kinetic models. pseudomonas acidovorans minera ... | 1985 | 3901918 |
| [plasmid participation in the degradation of alpha-methylstyrene]. | pseudomonas acidovorans 9 transforming alpha-methylstyrene into acetophenone contains four types of plasmid dna with molecular masses of 130, 110, 36 and 54 md. the loss of the "growth on alpha-methylstyrene" property by this strain correlates with the absence of plasmids with the molecular masses of 130 and 110 md from the cells. all the types of plasmid dna are found in transconjugants growing on alpha-methylstyrene and produced by crossing the parent p. acidovorans strain with the plasmidless ... | 1985 | 3937036 |
| tartaric acid metabolism. iv. crystalline l-malic dehydrogenase from pseudomonas acidovorans. | | 1968 | 4297260 |
| the role of imidazol-5-yl-lactate-nicotinamide-adenine dinucleotide phosphate oxidoreductase and histidine-2-oxoglutarate aminotransferase in the degradation of imidazol-5-yl-lactate by pseudomonas acidovorans. | | 1969 | 4303364 |
| tryptophan oxygenase of pseudomonas acidovorans. purification, composition, and subunit structure. | | 1969 | 4307451 |
| the metabolism of d-glucarate by pseudomonas acidovorans. | 1. dehydratases that converted d-glucarate into 4-deoxy-5-oxoglucarate were partially purified from klebsiella aerogenes and pseudomonas acidovorans. 2. when d-glucarate was metabolized to 2,5-dioxovalerate it appeared that water and carbon dioxide were removed from 4-deoxy-5-oxoglucarate in one enzymic step: 4,5-dihydroxy-2-oxovalerate was not an intermediate in this reaction. 3. a method for the enzymic determination of d-glucarate is described. | 1969 | 4311826 |
| the degradation of trans-ferulic acid by pseudomonas acidovorans. | | 1970 | 4312851 |
| pyrimidine metabolism in pseudomonas acidovorans. | | 1974 | 4364139 |
| studies on glucarate catabolism: the oxodeoxyglucarate aldolase activity of glucarate hydro-lyase from pseudomonas acidovorans. | glucarate hydro-lyase was isolated and purified to near homogeneity from cells of pseudomonas acidovorans grown on glucarate. by using gel filtration and ion-exchange chromatography, it was shown that the oxodeoxyglucarate aldolase activity observed in such extracts is associated with the glucarate hydro-lyase protein. | 1974 | 4447622 |
| studies on the biosynthesis of alpha-putrescinylthymine in bacteriophage phi w-14-infected pseudomonas acidovorans. | the alpha-putrescinylthymine (putthy) in bacteriophage phiw-14 dna is synthesized at the mononucleotide level: it is labeled by uracil or deoxyuridine but not by thymidine, and it appears in the acid-soluble pool of infected cells before the onset of phage dna synthesis. the methylene group at the c-5 position of the pyrimidine moiety of putthy is derived in vivo from a c(1) unit. extracts of a phage infected thymidine auxotroph of the host, pseudomonas acidovorans, apparently contain a phage-sp ... | 1973 | 4586777 |
| the regulation of the -ketoadipate pathway in pseudomonas acidovorans and pseudomonas testosteroni. | | 1972 | 4657135 |
| obligate thymidine auxotrophs of pseudomonas acidovorans. | the aminopterin technique was adapted for the isolation of thymidine auxotrophs of pseudomonas acidovorans. all the mutants isolated responded to thymidine but not to thymine. | 1973 | 4688666 |
| polyamines of pseudomonas acidovorans. | pseudomonas acidovorans contains putrescine, 2-hydroxyputrescine, and spermidine. | 1973 | 4712573 |
| precursors of polyamines in pseudomonas acidovorans. | | 1974 | 4828866 |
| purification and properties of d-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating). | 1. an enzyme extracted from pseudomonas acidovorans was purified and shown to catalyse the simultaneous dehydration and decarboxylation of d-4-deoxy-5-oxoglucarate. it is proposed to name the enzyme d-4-deoxy-5-oxoglucarate hydro-lyase (decarboxylating), trivial name ;deoxyoxoglucarate dehydratase'. 2. no added cofactors were required, and the enzyme was inactivated when incubated with its substrate in the presence of sodium borohydride. under these conditions the substrate and enzyme appeared t ... | 1969 | 4982840 |
| incorporation of molecular oxygen during the biosynthesis of ubiquinone in an aerobic bacterium, pseudomonas desmolytica. | | 1972 | 5006906 |
| the physical properties and mechanism of action of deoxyoxoglucarate dehydratase from pseudomonas acidovorans. | | 1969 | 5343786 |
| the function of the beta-ketoadipate pathway in pseudomonas acidovorans. | | 1969 | 5352018 |
| isolation and properties of a pseudomonas acidovorans bacteriophage. | | 1970 | 5415489 |
| enzymatic degradation of ureidoglycine by pseudomonas acidovorans. | | 1970 | 5423834 |
| synergistic and product induction of the enzymes of tryptophan metabolism in pseudomonas acidovorans. | the process of induction of tryptophan oxygenase in pseudomonas acidovorans is typical of many microbial enzyme induction systems, in that it (i) requires cell multiplication and de novo protein synthesis, (ii) is subject to catabolite repression, (iii) results in the formation of a stable enzyme, whose level, upon removal of inducer, is diluted out by cell proliferation, and (iv) exhibits product induction. l-kynurenine was more effective than l-tryptophan as an inducer of both tryptophan oxyge ... | 1969 | 5773024 |
| product induction in pseudomonas acidovorans of a permease system which transports l-tryptophan. | growth of pseudomonas acidovorans in the presence of l-tryptophan resulted in the appearance of a tryptophan transport system which was extremely sensitive to sodium azide or 2,4-dinitrophenol. asparagine-grown cells possessed no detectable tryptophan "permease" activity. substitution of l-kynurenine for l-tryptophan in the growth medium also induced the tryptophan permease activity, along with tryptophan oxygenase and kynurenine formamidase. this is the first reported example of the product ind ... | 1969 | 5773025 |
| oxidation of indole to indigotin by pseudomonas indoloxidans. | | 1965 | 5861333 |
| degradation of allantoin by pseudomonas acidovorans. | | 1966 | 5942430 |
| interaction between bacterial metabolites and some pesticides. i. interaction between the phenolic compounds produced by pseudomonas acidovorans and the herbicide venzar. | the phenolic compounds produced by pseudomonas acidovorans interacted with the herbicide venzar changing its phytotoxicity. bioassay with chlorella vulgaris and lettuce showed that the interaction of the bacterial phenols and venzar was synergistic or antagonistic depending on the concentration of the phenolic compounds its chemical structure and ph of the environment. the humic-like polymers isolated from the bacterial culture reduced to a small extent the phytotoxicity of venzar. it was found ... | 1984 | 6209926 |
| antimicrobial activity of desacetylcefotaxime alone and in combination with cefotaxime: evidence of synergy. | the level of antimicrobial activity of 3-desacetylcefotaxime was found to be approximately eightfold lower than that of cefotaxime. variation in the inhibitory effects of desacetylcefotaxime was species dependent. desacetylcefotaxime was 1-2 log2 dilutions more active than cefotaxime against pseudomonas acidovorans and pseudomonas cepacia. desacetylcefotaxime and cefotaxime were bactericidal, and their minimal inhibitory concentrations were influenced only slightly by increases in the inoculum c ... | 1982 | 6294785 |
| synthesis of thymine and alpha-putrescinylthymine in bacteriophage phi w-14-infected pseudomonas acidovorans. | host dna synthesis stopped about 10 min after the infection of pseudomonas acidovorans with bacteriophage phi w-14, but host dna was not degraded to acid-soluble fragments. the synthesis of host but not of phage dna was inhibited by 5-fluorodeoxyuridine. the nucleotide pools of infected cells did not contain dttp, and infection resulted in the appearance of dttpase activity. although ornithine labeled the alpha-putrescinylthymine residues of phi w-14 dna, ornithine-labeled nucleotides were not d ... | 1980 | 6445427 |
| dna synthesis in pseudomonas acidovorans infected with mutants of bacteriophage phi w-14 defective in the synthesis of alpha-putrescinylthymine. | normal levels of the hypermodified pyrimidine, alpha-putrescinylthymine, which is formed from hydhydroxymethyluracil at the polynucleotide level (maltman et al., j. virol. 34:354-359, 1984), are not required in bacteriophage luminal diameterw-14 dna for the dna to serve as a replicative template in luminal diameterw-14-infected cells. | 1984 | 6492260 |
| immunological aspects of humidifier fever. | an epidemiological investigation was made of eight employees working in a defined section of an office building who complained of febrile reactions accompanied by respiratory tract symptoms and fatigue. culture of the water from the humidifier for their section yielded strains of pseudomonas acidovorans and an unidentified pseudomonas species. in vitro studies showed that these strains activated the alternative pathway of the complement system in mg-egta supplemented serum, as evidenced by prope ... | 1984 | 6499837 |
| magnetic and natural circular dichroism of l-tryptophan 2,3-dioxygenases and indoleamine 2,3-dioxygenase. i. spectra of ferric and ferrous high spin forms. | the ferric form of l-tryptophan 2,3-dioxygenases from both pseudomonas acidovorans (atcc 11299b) and rat liver showed magnetic cd spectra ascribable to a high spin protohemoprotein at neutral ph, whereas the ferric indoleamine 2,3-dioxygenase from rabbit intestine exhibited a spectrum due to a mixture of high and low spin states under comparable conditions. upon addition of l-tryptophan, the spectra of the former enzymes changed to another type of high spin spectra, while the latter showed a mar ... | 1983 | 6600455 |
| polypeptide synthesis directed by bacteriophage phi w-14 and by mutants defective in dna synthesis. | the latent period of bacteriophage phi w-14 is approximately 65 min when the doubling time of its host, pseudomonas acidovorans, is 85 min. host protein synthesis is shut off relatively slowly, stopping approximately 25 min after infection. there are several phases of phage-specific polypeptide synthesis during the latent period: early polypeptides appear within 10 min after infection; middle polypeptides start to appear between 10 nd 30 min; late polypeptides appear after 30 min. the lengths of ... | 1984 | 6708173 |
| l-tryptophan 2,3-dioxygenase of a moderate thermophile, bacillus brevis. purification, properties and a substrate-mediated stabilization of the quaternary structure. | l-tryptophan 2,3-dioxygenase (l-tryptophan: oxygen 2,3-oxidoreductase ( decycling ), ec 1.13.11.11) from bacillus brevis, a moderately thermophilic bacteria, was purified to apparent homogeneity. the enzyme had a molecular weight of 110 000 and consisted of four subunits of equal molecular size. the enzyme exhibited the typical absorption spectra of a protohemoprotein . the amino acid composition and catalytic properties of the thermophilic enzyme were almost similar to those of its mesophilic c ... | 1984 | 6712960 |
| thymine metabolism in pseudomonas aeruginosa strain 1: the presence of a salvage pathway. | exogenous thymine was found to be taken up very slowly by pseudomonas aeruginosa in comparison to other pyrimidines, and most of it was catabolized by the cell. the existence of a functional, although inefficient, thymine salvage pathway was demonstrated and this pathway operated more effectively when de novo thymidine nucleotide biosynthesis was inhibited by trimethoprim or methotrexate. the mechanism of thymine salvage by p. aeruginosa appears to be different from that of escherichia coli and ... | 1982 | 6811694 |
| inhibition of p-hydroxybenzoate hydroxylase by anions: possible existence of two anion-binding sites in the site for reduced nicotinamide adenine dinucleotide phosphate. | certain anions were found to inhibit p-hydroxybenzoate hydroxylase from pseudomonas desmolytica. the inhibition was of competitive or mixed type with respect to nadph (apparent ki = 4-30 mm). among the anions, monovalent anions such as halogen ions and azide inhibited ionization of the phenolic hydroxyl group of the substrate (p-hydroxybenzoate) on binding with the enzyme . substrate complex of p-hydroxybenzoate hydroxylase, without dissociating the substrate from the enzyme. on the other hand, ... | 1983 | 6841328 |
| a histidine residue in p-hydroxybenzoate hydroxylase essential for binding of reduced nicotinamide adenine dinucleotide phosphate. | chemical modification with diethylpyrocarbonate (ethoxyformic anhydride) was examined to demonstrate the existence of an essential histidine residue at the nadph-binding site of p-hydroxybenzoate hydroxylase (ec 1.14.13.2) from pseudomonas desmolytica. among some ligands, nadph was noticeable in protecting the enzyme from the modification-caused inactivation. although several amino acid residues were modified during the inactivation process, inhibition of the enzyme could be correlated with modi ... | 1982 | 7061489 |
| degradation of 3-phenylbutyric acid by pseudomonas sp. | pseudomonas sp. isolated by selective culture with 3-phenylbutyrate (3-pb) as the sole carbon source metabolized the compound through two different pathways by initial oxidation of the benzene ring and by initial oxidation of the side chain. during early exponential growth, a catechol substance identified as 3-(2,3-dihydroxyphenyl)butyrate (2,3-dhpb) and its meta-cleavage product 2-hydroxy-7-methyl-6-oxononadioic-2,4-dienoic acid were produced. these products disappeared during late exponential ... | 1982 | 7118830 |
| biochemical genetics of tryptophan synthesis in pseudomonas acidovorans. | sixty independent tryptophan auxotrophs of pseudomonas acidovorans were isolated and characterized for nutritional response to intermediates of the pathway, accumulation of intermediates, and levels of tryptophan-synthetic enzymes. mutants for each of the seven proteins catalyzing the five steps of tryptophan synthesis were obtained. transductional analysis established three unlinked chromosomal regions: trpe, trpgdc, and trpfba. the order of the genes within the two clusters was not determined. ... | 1981 | 7240095 |
| bacteriophage phi w-14-infected pseudomonas acidovorans synthesizes hydroxymethyldeoxyuridine triphosphate. | the infection of pseudomonas acidovorans with bacteriophage phi w-14 leads to the gradual disappearance of dttp from the cells and to the appearance of hydroxymethy dutp (hmdutp). infected-cell contain dump hydroxymethylase and activities converting hmdump to humdudp and hmdutp. hydroxymethylase appears immediately after infection, reaching a maximum 20 min later. thymidylate synthase activity decreases to less than 10% of the preinfection level during the initial 40 min after infection. newly r ... | 1980 | 7373713 |
| regulation of the utilization of 4-hydroxybenzoate and vanillate in batch and continuous cultures of pseudomonas acidovorans. | | 1980 | 7396639 |
| use of a simplified cell blot technique and 16s rrna-directed probes for identification of common environmental isolates. | a simple technique in which rrna-targeted oligodeoxynucleotide probes are used to identify bacteria immobilized on membranes is described. by using colony lifts, bacteria are directly transferred from plates to untreated nitrocellulose membranes. alternatively, cells resuspended from colonies can be applied to membranes by using a vacuum manifold under high-salt conditions. blotted cells are baked and hybridized under stringent conditions by using standard protocols. treatment of blotted cells w ... | 1993 | 7504429 |
| isolation and characterization of a bacterium which utilizes polyester polyurethane as a sole carbon and nitrogen source. | various soil samples were screened for the presence of microorganisms which have the ability to degrade polyurethane compounds. two strains with good polyurethane degrading activity were isolated. the more active strain was tentatively identified as comamonas acidovorans. this strain could utilize polyester-type polyurethanes but not the polyether-type polyurethanes as sole carbon and nitrogen sources. adipic acid and diethylene glycol were probably the main degradation products when polyurethan ... | 1995 | 7781989 |
| central venous catheter-related infection due to comamonas acidovorans in a child with non-hodgkin's lymphoma. | | 1994 | 7811890 |
| microbial synthesis and properties of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in comamonas acidovorans. | comamonas acidovorans ds-17 was isolated from activated sludge and found to produce copolymers of 3-hydroxybutyrate (3hb) and 4-hydroxybutyrate (4hb) at 30 degrees c under growth-limited conditions. when 1,4-butanediol or 4-hydroxybutyric acid was used as the sole carbon source, a p(4hb) homopolymer was produced. random copolymers of 3hb and 4hb units were produced on the addition of glucose or 3-hydroxybutyric acid to the culture solution of 4-hydroxybutyric acid. the physical properties of p(3 ... | 1994 | 8011595 |
| pseudomonas acidovorans: a bacterium capable of mineralizing 2-chloroaniline. | prolonged adaptation of ca-alginate immobilized cells of pseudomonas acidovorans ca28 to a mixture of 3-chloroaniline (3-ca)1) and 2-ca and subsequently to 2-ca as sole substrate led to the isolation of another strain, termed ca50 with the additional capability of utilizing 2-ca as sole source of carbon, nitrogen, and energy. batch-degradation of 190 mg/l of 2-ca at ph 6.1 by this newly isolated strain was achieved within 3 days, at higher concentrations up to 0.6 g/l increasing lag-phases and d ... | 1994 | 8014846 |
| construction of a dna probe to detect isoquinoline-degrading bacteria. | to facilitate the cloning of dna encoding isoquinoline degradation an assay was developed that allowed the rapid visual scoring of the isoquinoline degradation phenotype of single colonies. transposon mutagenesis of one of the isolates. comamonas acidovorans iq3, was performed using tn5, and nine isq-mutants deficient in the ability to utilise isoquinoline as the sole nitrogen source were isolated. these mutants were also incapable of utilising the first metabolite of the isoquinoline degradatio ... | 1994 | 8076251 |
| synthesis and antimicrobial activity of sperabillin derivatives. | modification of sperabillins was carried out. the 2-amidinoethylamino moiety was removed by brief acidic hydrolysis. the 2,4-hexadienoyl moiety was hydrogenated to the hexanoyl moiety and this was cleaved by an enzymatic reaction using the cells of pseudomonas acidovorans ifo 13582. the 2-amidinoethylamino and the 2,4-hexadienoyl moieties were replaced with other groups. the derivative which was prepared by condensation of two molar amounts of dehexadienoylsperabillin a with (e,e)-muconic acid s ... | 1993 | 8514635 |
| degradation of cocaine by a mixed culture of pseudomonas fluorescens mber and comamonas acidovorans mblf. | a mixed culture that could utilize cocaine as the sole source of carbon and energy for growth was isolated by selective enrichment. the individual microorganisms within this mixed culture were identified as pseudomonas fluorescens (termed mber) and comamonas acidovorans (termed mblf). each microorganism was shown to be unable to grow to any appreciable extent on 10 mm cocaine in the absence of the other. c. acidovorans mblf was found to possess an inducible cocaine esterase which catalyzed the h ... | 1996 | 8572717 |
| occurrence of polyhydroxyalkanoic acid granule-associated proteins related to the alcaligenes eutrophus h16 ga24 protein in other bacteria. | fifty different polyhydroxyalkanoic acid (pha)-accumulating bacterial strains were investigated for the occurrence of phasin proteins bound to pha granules and related to the ga24 protein of alcaligenes eutrophus h16, by isolating pha granules and western blot analysis of granule-associated proteins employing antibodies raised against the ga24 protein. it could be demonstrated that th pha granules of many poly(3-hydroxybutyrate)-accumulating bacteria exhibited a similar protein pattern, and a pr ... | 1996 | 8598273 |