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coagulation in the sand crab (ovalipes bipustulatus).the coagulation mechanism of the sand crab (o. bipustulatus) has been investigated. from the coagulocytes (amoebocytes) present in the crab haemolymph (blood), fibrinogen (coagulogen) was isolated. it was shown to be homogeneous by electrophoresis on s.d.s. polyacrylamide gel and had a molecular weight similar to the a alpha-chain of human fibrinogen. unlike human fibrinogen. unlike human fibrinogen it cannot be dissociated by reduction. in fibrin polymerization, a crosslinking process takes pla ...1979505375
purification and characterization of the sand crab (ovalipes bipustulatus) coagulogen (fibrinogen).from the coagulocytes (amoebocytes) coagulogen (fibrinogen) was isolated, and purified on sephacryl s-200. the cell homogenate contained one major protein species with a minimum molecular weight of 70,000. this protein clotted in the presence of human thrombin, human factor xiii and ca++. the coagulogen contained no free thiol groups, however these were detectable in the reduced protein. using the cyanoethylation procedure, it was estimated that one coagulogen molecule contained two lysine resid ...19817245128
ultrastructure of the scolex and tentacles of the metacestode of polypocephalus species (cestoda: lecanicephalidae) from the blue-swimmer crab portunus pelagicus.the ultrastructure and histochemistry of the scolex of a polypocephalus species is described. this cestode is distinguishable from all known polypocephalus spp. in that it has approximately 400 tentacles and inhabits the musculature of the blue swimmer crab, portunus pelagicus. the metacestode has a large scolex, divided into an anterior par apicalis, and a posterior pars basalis. an elongate cercomer lies posteriorly to the scolex. the pars basalis lacks accessory suckers. the tegument of this ...19958847169
comparative analyses by hplc and the sodium channel and saxiphilin 3h-saxitoxin receptor assays for paralytic shellfish toxins in crustaceans and molluscs from tropical north west australia.the increased frequency and distribution of red tides requires the development of high-throughput detection methods for paralytic shellfish toxins (pst). community ethics also requires that there be a reduced reliance upon the standard mouse bioassay. a biomolecular assay such as the sodium channel 3h-saxitoxin binding assay can satisfy both of these requirements but may be compromised by cross-reactivity with the structurally unrelated tetrodotoxins (ttx). this study utilised the sodium channel ...19989620576
incidence of highly genetically diversified vibrio parahaemolyticus in seafood imported from asian countries.vibrio parahaemolyticus is an important pathogen of humans and aquacultured animals, especially in asian countries. in this study, we examined 686 samples of seafood imported from hong kong, indonesia, thailand and vietnam for v. parahaemolyticus. v. parahaemolyticus was recovered from 315 (45.9%) samples. the incidence of v. parahaemolyticus in products from hong kong and thailand was markedly higher than the incidence in products from indonesia and vietnam. the incidence rates in shrimp, crab, ...199910733249
flux of heavy metal accumulation in various organs of the intertidal marine blue crab, portunus pelagicus (l.) from the kuwait coast after the gulf war.the metal levels of arsenic, chromium, copper, lead, magnesium, manganese, selenium, vanadium, and zinc concentrations were determined in various body organs, viz., hepatopancreas, gills, gonad, gastric stomach, and muscle of the blue crab, portunus pelagicus (crustacea: decapoda) to assess the bioaccumulation of metals associated with petroleum input a decade after the 1991 gulf war oil spillage. sample solutions prepared were analyzed using an atomic absorption spectrophotometry. high concentr ...200111686643
copper acute toxicity tests with the sand crab emerita analoga (decapoda: hippidae): a biomonitor of heavy metal pollution in chilean coastal seawater? 200212177761
bacteriology of unshelled frozen blue swimming crab (portunus pelagicus).in this study, 45 (10 whole specimens and 35 frozen claws) frozen samples of portunus pelagicus imported into sicily (italy) from the west coast of africa were examined to assess their bacteriological characteristics and suitability for consumption. bacteriological examination was performed on two subsamples for each whole crab. the first was the body and claw muscle; the second was a pool of viscera and gills. in the case of frozen claws, each muscle claw was a sample. an aerobic plate count at ...200415083736
isolation and expression analysis of multiple isoforms of putative farnesoic acid o-methyltransferase in several crustacean species.farnesoic acid o-methyltransferase (famet) is the enzyme responsible for the conversion of farnesoic acid (fa) to methyl farnesoate (mf) in the final step of mf synthesis. multiple isoforms of putative famet were isolated from six crustacean species belonging to the families portunidae, penaeidae, scyllaridae and parastacidae. the portunid crabs portunus pelagicus and scylla serrata code for three forms: short, intermediate and long. two isoforms (short and long) were isolated from the penaeid p ...200716999957
biological studies on the blue crab portunus pelagicus and its parasitic infection in kuwaiti waters.the study investigated the parasites of symbiotic fauna of the blue crab, portunus pelagicus and the environmental factors, which was lacking in kuwait. p. pelagicus feeding habits was studied by examination of the digestive tract. the foregut (stomach) contents of 250 crabs (110 male & 140 female) collected from fish-market, souq-sharq, edelyia capital governorate were investigated. a barnacle, balanus sp. and staked barnacle octolasmis sp. (crustacea: cirripedia) were found strongly firmly to ...200717580579
the portuguese man-of-war as a food source for the sand crab (emerita pacifica). 194617733733
genetic heterogeneity of the blue swimming crab (portunus pelagicus) in thailand determined by aflp analysis.genetic diversity and population differentiation of the blue swimming crab (portunus pelagicus) in thailand, originating from ranong and krabi located in the andaman sea (west) and chanthaburi, prachuap khiri khan, and suratthani located in the gulf of thailand (east), were examined by aflp analysis. high genetic diversity of p. pelagicus in thai waters was found (n=72). the four primer combinations generated 227 aflp fragments, and the percentage of polymorphic bands in each geographic sample w ...200717879155
development of a species-diagnostic scar marker of the blue swimming crab (portunus pelagicus). 200718000751
comparative distribution of a putative egg-laying hormone in neural and reproductive tissues of four decapoda crustaceans.evidence for the presence of a putative egg-laying (elh) hormone has been previously described in the black tiger shrimp, penaeus monodon, so a further investigation was carried out to detect its presence in a range of decapoda crustaceans prior to a full molecular analysis. the crustaceans were represented by the australian fresh water yabbie, cherax destructor, the australian southern rock lobster, jasus edwardsii, the snow crab, chionoecetes opilio, and the blue swimmer crab, portunus pelagic ...200919184148
mitigating discard mortality from dusky flathead platycephalus fuscus gillnets.the mortalities and contributing parameters were estimated for key species discarded during commercial gillnetting (80 mm mesh) targeting dusky flathead platycephalus fuscus in a southeastern australian estuary. bycatches (1470 individuals from 16 species over 11 deployments) were assessed for their immediate mortalities onboard the gillnetter, before subsamples (570 individuals from 11 species) were discarded into cages and monitored for their short-term fate over 4 d. appropriate controls were ...200919694175
permanent genetic resources added to molecular ecology resources database 1 may 2009-31 july 2009.this article documents the addition of 512 microsatellite marker loci and nine pairs of single nucleotide polymorphism (snp) sequencing primers to the molecular ecology resources database. loci were developed for the following species: alcippe morrisonia morrisonia, bashania fangiana, bashania fargesii, chaetodon vagabundus, colletes floralis, coluber constrictor flaviventris, coptotermes gestroi, crotophaga major, cyprinella lutrensis, danaus plexippus, fagus grandifolia, falco tinnunculus, fle ...200921564933
parasite infection and sand coarseness increase sand crab (emerita analoga) burrowing time.parasites with indirect life cycles require trophic transmission from intermediate hosts to definitive (vertebrate) hosts. transmission may be facilitated if parasite infection alters the behavior of intermediate hosts such that they are more vulnerable to predation. vulnerability to predation may also be influenced by abiotic factors; however, rarely are the effects of parasites and abiotic factors examined simultaneously. the swash zone of sandy beaches is a particularly harsh environment. san ...201121959036
Permanent Genetic Resources added to Molecular Ecology Resources Database 1 August 2011-30 September 2011.This article documents the addition of 299 microsatellite marker loci and nine pairs of single-nucleotide polymorphism (SNP) EPIC primers to the Molecular Ecology Resources (MER) Database. Loci were developed for the following species: Alosa pseudoharengus, Alosa aestivalis, Aphis spiraecola, Argopecten purpuratus, Coreoleuciscus splendidus, Garra gotyla, Hippodamia convergens, Linnaea borealis, Menippe mercenaria, Menippe adina, Parus major, Pinus densiflora, Portunus trituberculatus, Procontar ...201222136175
identification of the major allergens of charybdis feriatus (red crab) and its cross-reactivity with portunus pelagicus (blue crab).tropomyosin and arginine kinase have been identified as the major allergens in multiple species of crab. charybdis feriatus is an important commercial crab in this country.201223393908
observations on intraspecific variation of total protein, amino acid and total nonprotein compound levels and their fractions in the muscles, hepatopancreas and gonads of two crustaceans, portunus pelagicus (l.) and penaeus kerathurus (l.), in relation to maturation. ii. total nonprotein compounds and their fractions. 20113198021
a kinetic study of dna and basic protein metabolism during spermatogenesis in the sand crab, emerita analoga.cytochemical and radioautographic techniques define and confirm a staging scheme for developing spermatids of the decapod crab, emerita analoga. quantitative photometric data demonstrate that developing spermatids lose a significant proportion of their nuclear proteins, as evidenced by diminishing binding of fluorodinitrobenzene. photometric results also show that much (but not all) of the spermatid nuclear protein loss is in somatic-type histone, as evidenced by a dramatic fall in the histone/d ...20144109924
moult cycle specific differential gene expression profiling of the crab portunus pelagicus.crustacean moulting is a complex process involving many regulatory pathways. a holistic approach to examine differential gene expression profiles of transcripts relevant to the moulting process, across all moult cycle stages, was used in this study. custom cdna microarrays were constructed for portunus pelagicus. the printed arrays contained 5000 transcripts derived from both the whole organism, and from individual organs such as the brain, eyestalk, mandibular organ and y-organ from all moult c ...201121396120
gene expression profiling of cuticular proteins across the moult cycle of the crab portunus pelagicus.crustaceans represent an attractive model to study biomineralization and cuticle matrix formation, as these events are precisely timed to occur at certain stages of the moult cycle. moulting, the process by which crustaceans shed their exoskeleton, involves the partial breakdown of the old exoskeleton and the synthesis of a new cuticle. this cuticle is subdivided into layers, some of which become calcified while others remain uncalcified. the cuticle matrix consists of many different proteins th ...200717925039
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
multifunctional role of β-1, 3 glucan binding protein purified from the haemocytes of blue swimmer crab portunus pelagicus and in vitro antibacterial activity of its reaction product.β-1, 3 glucan binding protein (β-gbp) was isolated from the haemocytes of blue swimmer crab, portunus pelagicus and purified by laminarin coupled sephadex g-100 affinity column chromatography. the purified β-gbp has the molecular mass of 100 kda, confirmed by sds-page. the x-ray diffraction analysis of purified β-gbp indicates the crystalline nature of the protein and also the presence of single peak confirming the existence of β-glucan molecule. the results of agglutination assay showed that th ...201626611720
mercury contamination in some marine biota species from khuzestan shore, persian gulf.in this study, concentrations of mercury (hg) were analyzed in some marine biota species (fish, shrimp, and crab) from khuzestan shore, north part of the persian gulf. it was also our intention to evaluate potential risks to human health associated with seafood consumption. the results indicated that concentrations of hg in the fish and crustacean were different among the species and tissues. liver in fish and hepatopancreas in crustacean exhibited higher hg concentration than the other tissues. ...201625500758
characterization of the complete mitochondrial genome of portunus pelagicus with implications for phylogenomics.this study determined the mitochondrial genome structure of the blue swimming crab (portunus pelagicus), and elucidated its phylogenetic relationships among the species within the order decapoda. the complete mitochondrial genome was 16,155 bp long, and contained 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and 1 dna control region. the gene order of the genome was the same as that found within the family portunidae. twenty-three genes were on the heavy strand and 14 we ...201627706563
localization and identification of crustacean hyperglycemic hormone producing neurosecretory cells in the eyestalk of blue swimmer crab, portunus pelagicus.this study intensely focuses on to the localization and identification of crustacean hyperglycemic hormone (chh) producing neurosecretory cells in the eyestalk of the blue swimmer crab portunus pelagicus. anti-carcinus maenas-chh was used to identify the location of chh neurosecretory cells by immunohistochemistry. ten pairs of eyestalks were collected from intact adult intermoult female crab and fixed in bouin's fixative. eyestalks were serially sectioned and stained with chrome-hematoxylin-phl ...201627460068
chromatographic analysis of low-molecular-mass copper-binding ligands from the crab species scylla serrata and portunus pelagicus.two copper-binding proteins and a zinc-binding ligand were isolated from the hepatopancreas of the crab portunus pelagicus. the copper-binding proteins behave similarly to those from the crabs carcinus maenas and scylla serrata, and were shown to be metallothioneins. reversed-phase high-performance liquid chromatographic (hplc) analysis confirmed the relative purity of both proteins with only cross-contamination between the two different forms of metallothioneins, and offers a good method to sep ...19921618990
variation in the abundance of pseudo-nitzschia and domoic acid with surf zone type.most harmful algal blooms (hab) originate away from the shore and, for them to endanger human health, they must be first transported to shore after which they must enter the surf zone where they can be feed upon by filter feeders. the last step in this sequence, entrance into the surf zone, depends on surf zone hydrodynamics. during two 30-day periods, we sampled pseudo-nitzschia and particulate domoic acid (pda) in and offshore of a more dissipative surf zone at sand city, california (2010) and ...201628073530
the influence of environmental factors on the abundance and recruitment of the sand crab emerita analoga (stimpson 1857): source-sink dynamics?the sandcrab emerita analoga is the dominant species inhabiting sandy beaches along the pacific coast of the american continent. in our study, 10 sandy beaches were sampled seasonally from 2006 to 2011, including coastal planktonic sampling from 2006 to 2008. two major population cores were detected, the first one in the northern part of the study area and the second in the area immediately to the south of the itata river mouth. zoeal stages were found along the entire coastal zone. highest dens ...201323688579
habitat-related divergence among tailfan sensory systems in reptantian decapod crustaceans.squat lobsters (galatheidae) and mole sand crabs (hippidae) differ in posture and locomotion from each other and from crayfish, their surrogate ancestor for neurobehavioral features. galatheids resemble crayfish more closely in general behavior and niche, but are intermediate between crayfish and hippids with respect to morphology and neuromusculature. the tailfan is inverted under the abdomen in both, due to the flexed abdominal posture, but its morphology has diverged considerably. nothing is ...200919494487
associations between shorebird abundance and parasites in the sand crab, emerita analoga, along the california coast.i investigated spatial variation in the prevalence and abundance of 4 species of parasites in the sand crab, emerita analoga, on 8 sandy beaches along 800 km of the california coast, to assess the importance of bird abundance for the distribution of parasites among sand crab populations. i collected sand crabs and counted shorebirds and gulls at each beach during june and november 1994. sand crabs served as intermediate hosts for 4 species of parasites, including a trematode, spelotrema nicolli ...200717539408
neuronal network models of phase separation between limb cpgs of digging sand crabs.ordinary differential equations are used to model a peculiar motor behaviour in the anomuran decapod crustacean emerita analoga. little is known about the neural circuitry that permits e. analoga to control the phase relationships between movements of the fourth legs and pair of uropods as it digs into sand, so mathematical models might aid in identifying features of the neural structures involved. the geometric arrangement of segmental ganglia controlling the movements of each limb provides an ...200616673144
projection and local interneurons in the sixth abdominal ganglion of the sand crab emerita analoga (hippidae).hippid crabs are adapted for life in the surf zone of exposed sandy beaches, and their tailfan differs from the tailfans of other crustaceans with respect to morphology and motor control and in having nonspiking stretch receptors (nsr). to investigate how these crabs' mechanosensory systems are adapted to this turbulent environment, i used axonal back-filling and intracellular recording with dye-filled microelectrodes to describe afferent projections from the telson and morphologies and physiolo ...200415515176
emerita analoga (stimpson)--possible new indicator species for the phycotoxin domoic acid in california coastal waters.blooms of domoic acid (da) synthesizing diatoms (pseudo-nitzschia spp.) have been associated with the death and injury of hundreds of marine shorebirds and mammals, exposed humans to potentially serious health risks, and threatened to significantly impact coastal fisheries and commerce dependent on marine resources. while indicator organisms are widely utilized to monitor for marine biotoxins like paralytic shellfish poisoning toxins, a reliable intertidal indicator species to monitor da remains ...200212220710
emerita analoga (stimpson) as an indicator species for paralytic shellfish poisoning toxicity along the california coast.paralytic shellfish poisoning toxins (pspt) produced by certain harmful algal blooms (habs) pose a serious threat to public health worldwide. along the west coast of north america, mytilus californianus (california sea mussel) has long been used as the primary indicator species for monitoring levels of pspt in the environment. however, because the natural distribution of this species is limited to rocky shores, vast stretches of coastline bordered by sandy beach habitat are not regularly monitor ...200212165323
development of a protocol for determination of domoic acid in the sand crab (emerita analoga): a possible new indicator species.the aim of this study was to begin evaluating the utility of sand crabs (emerita analoga) as an indicator species for the algal neurotoxin, domoic acid (da), in monterey bay, california, usa, a site of recurrent blooms of the da-producing diatom, pseudo-nitzschia. one of the current sentinel organisms, the sea mussel (mytilus californianus), has shown minimal or undetectable toxicity during some local bloom events. as a critical step in assuring the accuracy of da determinations in e. analoga, w ...200211821119
receptor potentials and electrical properties of nonspiking stretch-receptive neurons in the sand crab emerita analoga (anomura, hippidae).receptor potentials and electrical properties of nonspiking stretch-receptive neurons in the sand crab emerita analoga (anomura, hippidae). four nonspiking, monopolar neurons with central somata and large peripheral dendrites constitute the sole innervation of the telson-uropod elastic strand stretch receptor in emerita analoga. we characterized their responses to stretch and current injection, using two-electrode current clamp, in intact cells and in two types of isolated peripheral dendritic s ...199910322084
sensitivity of the sand crab emerita analoga to a weathered oil. 199910094731
digging in sand crabs: coordination of joints in individual legs.sand crabs use their multi-jointed legs to dig into sand. combined movement and electromyogram (emg) analyses showed that the pattern of intra-leg coordination in the legs of two sand crabs of different families (blepharipoda occidentalis and emerita analoga) is similar in legs 2 and 3, but very different in leg 4. for example, the sequence of proximal joint movements in legs 2 and 3 is elevation, retraction, depression and protraction (similar to backward walking in most decapods), but the sequ ...19989639588
distribution and regulation of esterases that hydrolyze methyl farnesoate in homarus americanus and other crustaceans.ester hydrolysis of methyl farnesoate (mf) by crustacean tissue homogenates was measured using the substrate [3h]mf in a radiochemical partition assay. tissues were obtained from the lobster homarus americanus, penaeid shrimp sicyonia ingentis, thalanassid shrimp callianassa californiensis, sand crab emerita analoga, and spider crab pugettia producta. the greatest specific activities were recovered from the hepatopancreas (239 to 11,500 pmol mf/min-mg total protein). hepatopancreatic homogenates ...19979126466
digging in sand crabs (decapoda, anomura, hippoidea): interleg coordinationsand crabs (decapoda, anomura, hippoidea) are highly specialised for digging into sand using their thoracic legs. using video-recording and electromyography, we examined the digging leg movements of three species of sand crabs belonging to two families: blepharipoda occidentalis (albuneidae), lepidopa californica (albuneidae) and emerita analoga (hippidae). the digging patterns of all three species are similar. the ipsilateral legs 2 and 3 are tightly coupled and shovel sand forward from underne ...19979318562
a map of distal leg motor neurons in the thoracic ganglia of four decapod crustacean species.we describe the numbers, central positions, and axonal exit routes of the distal leg motor neurons of four decapod species: squat lobsters (munida quadrispina), spiny sand crabs (blepharipoda occidentalis), mole sand crabs (emerita analoga), and signal crayfish (pacifastacus leniusculus). as predicted by previous physiological and anatomical identification of axons at the periphery in crayfish and lobsters, cobalt backfills reveal about seventeen cell bodies, which are found in four areas in the ...19979063594
replacement of an inherited stretch receptor by a newly evolved stretch receptor in hippid sand crabs.primary sensory neurons that are motoneuron-like in morphology and often nonspiking (transmit afferent signals as graded depolarizations) characterize an unusual type of stretch receptor in decapod crustaceans. nonspiking and spiking receptors occur in similar positions at homologous joints in different species and have been presumed to be homologous, the spiking one considered "primitive". to better understand the evolutionary origin of these stretch receptors and why some are nonspiking, we ex ...19947860798
functional morphology of the telson-uropod stretch receptor in the sand crab emerita analoga.the telson-uropod stretch receptor in emerita analoga belongs to a segmental array of axial-coxal receptors having sensory neurons with central somata. it consists of an elastic strand innervated by four giant, nonspiking, mechanoreceptive neurons. we examined the elastic (sensory) strand by light and electron microscopy to discover whether 1) functional differences between the four sensory neurons could be surmised from their peripheral terminations and 2) the process of mechano-electric transd ...19902358541
factors influencing aggregation patterns in the sand crab emerita analoga (crustacea: hippidae).aggregation patterns in emerita analoga (in southern california) are delineated with respect to their spatial, daily, and seasonal components. both abiotic and biotic factors are found to be associated with patterns of aggregation. spatially, e. analoga aggregates from march through september to a significantly greater degree in the upper one-third area of the wash zone where exposure to wave shock and fish predation are probably decreased. sand crabs are more aggregated on a daily basis during ...198028309568
an endogenous motor program for sand crab uropods.a stereotyped pattern of spontaneous, rhythmic bursting in motoneurons of three principal uropod muscles in the sand carb emerita analoga has been recorded from a deafferented chain of the four most-posterior abdominal ganglia. this endogenous motor program resembles the electromyogram pattern recorded from return-stroke and power-stroke muscles in swimming crabs in that (1) latencies of power-stroke bursts and burst periods are positively correlated with each other and (2) durations of power-st ...1979458439
role of proprioceptive feedback from nonspiking mechanosensory cells in the sand crab, emerita analoga.the two programmes underlying uropod beating in emerita ('swimming' and 'treading water') are distinguished by a difference in timing of the power stroke within the interval between return strokes (cycle). while 'swimming' (cycles approx. 80-400 ms) the phase and while 'treading water' (cycles approx. 100-700 ms) the latency of the power stroke are relatively invariant. analyses of electromyograms of untrestrained emerita, before and after bilateral surgical ablation of the four nonspiking mecha ...1976993703
species diversity and abundance of marine crabs (portunidae: decapoda) from a collapsible crab trap fishery at kung krabaen bay, chanthaburi province, thailand.the diversity and abundance of marine crabs from a collapsible crab trap fishery at kung krabaen bay, gulf of thailand, were observed from august 2012 to june 2013 using 10 sampling stations. the results showed that there were seven families, 11 genera and 17 species (two anomuran and 15 brachyuran crabs). the two anomuran species were clibanarius virescens (1,710 individuals) and clibanarius infraspinatus (558 individuals). for brachyuran crabs, portunidae was the most common family, including ...201728228916
a stock assessment of the blue swimming crab portunus pelagicus (linnaeus, 1758) for sustainable management in kung krabaen bay, gulf of thailand.a stock assessment of blue swimming crabs, portunus pelagicus was conducted with crab gill nets and collapsible crab traps at kung krabaen bay, in the eastern gulf of thailand, from 2008 to 2009. several key indicators show that p. pelagicus population is in crisis. fishing mortality shows an increase to 4.14. the exploitation rate is 0.71, higher than the optimal value of 0.38. the size of the mature females has also decreased from 8.10±0.39 cm to 7.52±1.14 cm. the average fecundity is 0.572×10 ...201425210587
[mass spectrometry identification and immune cross-reactivity of a minor shrimp allergen-sarcoplasmic calcium binding protein from litopenaeus vannamei].to identify sarcoplasmic calcium-binding protein (scp) as a minor shrimp allergen by mass spectrometry, and to analyze the immune cross-reactivity among crustacean scps.201222863586
growth inhibition and antibiofilm potential of ag nanoparticles coated with lectin, an arthropod immune molecule.lectins from the haemolymph of arthropods, including crustaceans, are molecules potentially involved in the immune recognition and phagocytosis. here, lectin was purified from the haemolymph of blue swimmer crab portunus pelagicus, using mannose-coupled sepharose cl-4b affinity column chromatography. in sds-page analysis, lectin showed a molecular mass of approximately 155kda. the synthesis of lectin-coated silver nanoparticles (lectin-coated agnps) was confirmed by uv-vis spectroscopy, xrd, fti ...201728441606
characterization and antimicrobial evaluation of sppr-amp1, a proline-rich antimicrobial peptide from the mud crab scylla paramamosain.antimicrobial peptide (amp) is an important molecule in the innate immune system. here, we report the cloning and functional studies of proline-rich amps (pr-amps) from the three species of mud crab: scylla paramamosain, s. serrata, and the swimming crab portunus pelagicus. the deduced peptides revealed that they contain the putative signal peptides and encode for mature peptides, which contain sequence architecture similar to a 6.5-kda proline-rich amp of the shore crab, carcinus maenas which s ...201728479344
effect of thermal processing on t cell reactivity of shellfish allergens - discordance with ige reactivity.crustacean allergy is a major cause of food-induced anaphylaxis. we showed previously that heating increases ige reactivity of crustacean allergens. here we investigate the effects of thermal processing of crustacean extracts on cellular immune reactivity. raw and cooked black tiger prawn, banana prawn, mud crab and blue swimmer crab extracts were prepared and ige reactivity assessed by elisa. mass spectrometry revealed a mix of several allergens in the raw mud crab extract but predominant heat- ...201728273149
management adaptation of invertebrate fisheries to an extreme marine heat wave event at a global warming hot spot.an extreme marine heat wave which affected 2000 km of the midwest coast of australia occurred in the 2010/11 austral summer, with sea-surface temperature (sst) anomalies of 2-5°c above normal climatology. the heat wave was influenced by a strong leeuwin current during an extreme la niña event at a global warming hot spot in the indian ocean. this event had a significant effect on the marine ecosystem with changes to seagrass/algae and coral habitats, as well as fish kills and southern extension ...201628725352
ige reactivity of blue swimmer crab (portunus pelagicus) tropomyosin, por p 1, and other allergens; cross-reactivity with black tiger prawn and effects of heating.shellfish allergy is a major cause of food-induced anaphylaxis, but the allergens are not well characterized. this study examined the effects of heating on blue swimmer crab (portunus pelagicus) allergens in comparison with those of black tiger prawn (penaeus monodon) by testing reactivity with shellfish-allergic subjects' serum ige. cooked extracts of both species showed markedly increased ige reactivity by elisa and immunoblotting, and clinical relevance of ige reactivity was confirmed by baso ...201323840718
evaluate of heavy metal concentration in shrimp (penaeus semisulcatus) and crab (portunus pelagicus) with inaa method.the level of the heavy metal in green tiger shrimp (penaeus semisulcatus) and crab (portunus pelagicus) caught off the persian gulf near bushehr province were investigated. this study was performed to evaluate instrumental neutron activation analysis (inaa) to analyze heavy metal concentration in crab and shrimp whole body tissue. the order of the swimmer crab and shrimp heavy metal concentrations were zn>fe>as>mn>co and fe>zn>mn>as>co, respectively. the results showed swimmer crab (portunus pel ...201323487484
filtering out parasites: sand crabs (lepidopa benedicti) are infected by more parasites than sympatric mole crabs (emerita benedicti).two digging decapod crustaceans, the sand crab species lepidopa benedicti and the mole crab species emerita benedicti, both live in the swash zone of fine sand beaches. they were examined for two parasites that infect decapod crustaceans in the region, an unidentified nematode previously shown to infect l. benedicti, and cestode tapeworm larvae, polypocephalus sp., previously shown to infect shrimp (litopenaeus setiferus). lepidopa benedicti were almost always infected with both parasite species ...201728951818
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