Publications

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interspecific differentiation of trypanosoma cruzi, trypanosoma conorhini and trypanosoma rangeli by lectins in combination with complement lysis.four-day-old epimastigote culture forms of trypanosoma cruzi, trypanosoma rangeli and trypanosoma conorhini were tested with 21 lectins. furthermore t. conorhini was incubated with the following sera: rat, wistar han, germ free; normal fresh hen, rat and human serum. t. rangeli was agglutinated only by the d-mannose specific lectins from canavalia ensiformis and pisum sativum. t. cruzi and t. conorhini could be distinguished by the lectin from tridacna crocea. the epimastigote culture forms of t ...19846143480
differentiation of trypanosoma cruzi chagas, 1909 and trypanosoma vespertilionis battaglia, 1904 by various lectins.four-days-old culture forms of trypanosoma cruzi (strain téhuantépéc, guatemala) and trypanosoma vespertilionis (strain p-14, p-9) were tested by 19 carbohydrate-specific agglutinins. the t. cruzi strains are interspecifically distinguishable with the lectins from euonymus europaeus, tridacna crocea, tridacna maxima and the human blood-group testserum anti-b from the t. vespertilionis strains. while the t. vespertilionis strains did react with anti-b and e. europaeus, the t. cruzi strains did no ...19836349060
phylogeny of gregarines (apicomplexa) as inferred from small-subunit rdna and beta-tubulin.gregarines are thought to be deep-branching apicomplexans. accordingly, a robust inference of gregarine phylogeny is crucial to any interpretation of apicomplexan evolution, but molecular sequences from gregarines are restricted to a small number of small-subunit (ssu) rdna sequences from derived taxa. this work examines the usefulness of ssu rdna and beta-tubulin sequences for inferring gregarine phylogeny. ssu rrna genes from lecudina (mingazzini) sp., monocystis agilis stein, leidyana migrato ...200312656194
morula-like cells in photo-symbiotic clams harboring zooxanthellae.symbiosis is observed between zooxanthellae, symbiotic dinoflagellates, and giant clams and related clams which belong to the families tridacnidae and cardiidae. we have previously shown that a photo-symbiotic clam tridacna crocea has three types of hemocytes, the eosinophilic granular hemocyte with phagocytic activity, the agranular cell with electron lucent granules, and the morula-like cell with large (ca. 2 mum in diameter) colorless granules. the function of the morula-like cell is not clea ...199818465996
perkinsus olseni detected in vietnamese aquacultured reef clams tridacna crocea imported to the usa, following a mortality event.morbidity and mortality were observed in a group of 30 reef clams tridacna crocea that were imported to florida, usa, from a vietnamese culture facility and held in research facility aquaria. clinical signs included an incompletely extended mantle, slow mantle responses to stimuli, and sloughing of byssal tissue beginning 2 to 5 d prior to death. necropsy findings included emaciation, visceral mass edema, and rare multifocal 1 mm off-white to light-tan gill nodules. histopathology revealed marke ...200818589999
exotic perkinsus sp. protozoa in an imported vietnamese ornamental clam (tridacna crocea) maintained in a home aquarium.an adult, hermaphroditic tridacna crocea ornamental clam imported from vietnam into the usa became terminally moribund with sloughed byssal tissue and incomplete extension of the poorly responsive mantle and was necropsied. necropsy findings included emaciation, visceral mass edema, and rare multifocal, 1-mm diameter, off-white to light tan gill nodules. histopathology revealed marked inflammation and necrosis within the visceral mass and gills, with interstitial edema and atrophy of glandular, ...200919368253
differential production of active oxygen species in photo-symbiotic and non-symbiotic bivalves.we investigated the generation of active oxygen species in the bivalves, crassostrea gigas, fulvia mutica and tridacna crocea in order to understand the defensive mechanisms in giant clams that allow a stable association with symbiotic zooxanthellae. c. gigas produced active oxygens, superoxide anion and nitric oxide upon stimulation by phorbol myristate acetate. f. mutica generated a little amount of superoxide anion and nitric oxide, and contained significant phenoloxidase activity which catal ...20139639085
margolisiella islandica sp. nov. (apicomplexa: eimeridae) infecting iceland scallop chlamys islandica (müller, 1776) in icelandic waters.wild iceland scallops chlamys islandica from an icelandic bay were examined for parasites. queen scallops aequipecten opercularis from the faroe islands and king scallops pecten maximus and queen scallops from scottish waters were also examined. observations revealed heavy infections of eimeriorine parasites in 95-100% of c. islandica but not the other scallop species. all life stages in the apicomplexan reproduction phases, i.e. merogony, gametogony and sporogony, were present. trophozoites and ...201121856309
cellular identity of a novel small subunit rdna sequence clade of apicomplexans: description of the marine parasite rhytidocystis polygordiae n. sp. (host: polygordius sp., polychaeta).a new species of rhytidocystis (apicomplexa) is characterized from north american waters of the atlantic ocean using electron microscopy and phylogenetic analyses of small subunit (ssu) rdna sequences. rhytidocystis polygordiae n. sp. is a parasite of the polychaete polygordius sp. and becomes the fourth described species within this genus. the trophozoites of r. polygordiae were relatively small oblong cells (l=35-55 microm; w=20-25 microm) and distinctive in possessing subterminal indentations ...200616872296
specificity of a model cnidarian-dinoflagellate symbiosis.to understand the flexibility of symbiotic associations in coral reefs, we investigated the specificity of the aiptasia (cf. insignis)-symbiodinium association in the laboratory by rendering the anemones aposymbiotic and inoculating them with different isolates of symbiodinium: infective algal symbionts were monitored over 3 months by re-isolation and identification using denaturing-gradient gel electrophoresis and sequence comparison of their amplified 18s rrna hypervariable v1 + v2 gene region ...200211842017
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