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[studies on echinostoma spp. in the chungju reservoir and upper streams of the namhan river].the present study was performed to know the epidemiological status of echinostomiasis in the chungju reservoir and upper streams of namhan river, together with an experimental study on the life history of echinostoma hortense. the stool specimens of 169 inhabitants and 473 junior high school students from 5 different villages revealed 3 (0.5%) echinostomatid egg positive cases. e. hortense adult worms were recovered from one patient after a treatment and purgation. for the other two patients, it ...19902099176
[study on centrocestus armatus in korea. i. infection status of zacco platypus and z. temminckii with the metacercariae of c. armatus].this study was performed to observe the infection rate and infection intensity of fresh water fish such as pale chub (z. platypus) and dark chub (z. temminckii) with the metacercariae of c. armatus. the fish were caught in several rivers and streams from june to september, 1988 and examined by artificial digestion technique. total 370 fish were caught at 19 rivers. the metacercarial (c. armatus) infection rate and average burden in z. platypus were 86.8% and 224, and those in z. temminckii were ...19892487262
study on centrocestus armatus in korea. ii. recovery rate, growth and development of worms in albino rats.some biological characteristics of centrocestus armatus were studied using albino rats as its experimental host. the metacercariae were collected from zacco platypus by artificial digestion method. laboratory rats (wistar) were fed each 100 or 200 metacercariae and sacrificed on 1, 2, 3, 4, 5, 6, 8, 14 and 28 days after infection to recover worms of various ages. the average recovery rate was 10.7% from 82 rats. the rate decreased rather slowly for the first 8 days but showed a steep decrease th ...19892487263
clonorchis sinensis: life cycle, intermediate hosts, transmission to man and geographical distribution in korea.the snail host of clonorchis sinensis in korea is referred to as parafossarulus manchouricus, but it has been recorded also as bulimus striatulus japonicus in literature published up to late 1950. the specific name of the snail was corrected such that bulimus striatulus of china, japan, and korea should be incorporated in the species of p. manchouricus. in general, the snail exists in limited areas of the rivers han, kum, mankyung, naktong, and yeongsan, and the infestation of the snail with the ...19846391501
clonorchiasis and metagonimiasis in the inhabitants along talchongang (river), chungwon-gun.to evaluate the status of clonorchiasis and metagonimiasis of the inhabitants near talchongang (river) in chungwon-gun, chungchongbuk-do, the stools of 67 inhabitants were examined by formalin-ether sedimentation method from august to september, 1993. also freshwater fish caught in talchongang were examined by slide compression method. the egg positive cases of clonorchis sinensis and metagonimus sp. were 22 (32.8%), and 14 (20.9%), respectively. of 17 species of examined fish. 14 species were i ...19947834244
[intestinal flukes of genus metagonimus and their second intermediate hosts in kangwon-do].this study investigate the epidemiological feature of metagonimus infection in kangwon-do (province). the average infection rate of the surveyed inhabitants was 7.8% (83 positives out of 1, 067 examinees) by stool examination; male, 11.4% and female, 3.2%, respectively. the egg positive rate in residents in the som river area was 7.3%, that of the chuchon river area 6.3%, the pyongchang river area 12.8%, the tong river area 3.8%, the hongchon river area 9.8%, and the ohsip stream area of samchok ...19938297890
an epidemiological study of metagonimiasis along the upper reaches of the namhan river.an epidemiological study of metagonimus infection was undertaken along the upper reaches of the namhan river, with special consideration on the species (type) of the worms collected from humans. eggs of metagonimus spp. were detected from 15 (9.7%) of 154 people examined in umsong-gun, and from each infected person (5 cases) 6,015-24,060 worms (mean 13,233) were recovered after treatment with praziquantel (10 mg/kg). eggs were also detected from 37 (48.1%) of 77 people in yongwol-gun, from whom ...19938343462
classification and host specificity of metagonimus spp. from korean freshwater fish.taxonomic problems of metagonimus spp. in korea were investigated. metacercariae of various freshwater fish species -- plecoglossus altivelis, carassius auratus, zacco platypus, zacco temmincki, opsariichthys bidens -- were collected from different localities in korea and experimentally fed to golden hamsters. observation of recovered adult worms showed that plecoglossus altivelis was infected with metacercariae of both m. yokogawai and m. takahashii. c. auratus was infected with metacercariae o ...19968820736
the infestation states and changing patterns of human infecting metacercariae in freshwater fish in kyongsang-do and kyonggi-do, korea.the infestation rates and abundances of human infecting metacercariae (clonorchis sinensis, metagonismus spp., centrocestus armatus, echinostoma hortense, echinochasmus japonicus, clinostomum complanatum) in freshwater fish were investigated at the three river areas-taewhagang (river), hyongsangang (river), nakdonggang (river) -in kyongsang-do and at four streams-yonpungchon, munsanchon, kyonganchon, and konjiamchon-in kyonggi-do, korea in 1994-1995. the fish caught at taewhagang were heavily in ...19968925250
metagonimus miyatai sp. nov. (digenea: heterophyidae), a new intestinal trematode transmitted by freshwater fishes in japan and korea.metagonimus miyatai n. sp. (digenea: heterophyidae) is described based on adult flukes collected from experimental dogs and hamsters fed with metacercariae encysted in the sweetfish, plecoglossus altivelis, dace, tribolodon hakonensis and t. taczanowskii, common fat-minnow, morocco steindachneri, pale chub, zacco platypus, and dark chub, zacco temmincki, and on those collected from naturally infected humans. the new species was morphologically compared with m. yokogawai and m. takahashii obtaine ...19979446902
expression patterns of proliferating cell nuclear antigen in the small intestine of mice infected with metagonimus yokogawai and metagonimus miyata type.genus metagonimus has been a subject of taxonomic debates for several years. in morphological aspects, m. yokogawai has been thought to have three subtypes, namely yokogawa type (m. yokogawai in strict sense), miyata type and koga type, but differences in the intestinal pathology induced by these subtypes have not been studied yet. in this study we compared the pathological reactions induced by m. yokogawai and metagonimus miyata type using proliferating cell nuclear antigen (pcna) index. metace ...19979446904
development of genotoxicity assay systems that use aquatic organisms.our aim is to develop and evaluate monitoring systems that use aquatic organisms to assess the genotoxicity of water in the field and in the laboratory. in a field study, we have shown that the micronucleus assay is applicable to freshwater and marine fishes and that gill cells are more sensitive than hematopoietic cells to micronucleus-inducing agents. gill cells from carassius sp. (funa) and zacco platypus (oikawa) collected upstream on the tomio river (nara, japan), tended to have lower micro ...19989672655
surface ultrastructure of metagonimus miyatai metacercariae and adults.a scanning electron microscopic study was performed to observe surface ultrastructures of excysted metacercariae and adults of metagonimus miyatai. metacercariae were collected from the scale of the pale chub (zacco platypus), and adult flukes were harvested 1-4 weeks after infection to rats. in excysted metacercariae, the oral sucker was devoid of tegumental spines and had type i and type ii sensory papillae. anteriorly to the ventral sucker, spines were dense and digitated into 5-7 points, whe ...19989868886
a human case of centrocestus armatus infection in korea.a human case of centrocestus armatus (heterophyidae) infection was proved by identifying an adult worm collected after treatment with praziquantel in korea. the case is 42-year old man who resides in a rural area in sanchung-gun, kyeongsangnam-do. the case was concomitantly infected with clonorchis sinensis and had the history of eating raw freshwater fishes including zacco platypus, which are known to be the second intermediate host of c. armatus in korea. this is the first report of natural hu ...198812811033
changing patterns of infection with digenetic larval trematodes from fresh-water fish in river taewha, kyongnam province.recent patterns of infection with digenetic larval trematodes in fresh-water and brackish-water fish were studied in three locations of the river taewha during the period from april to october 1988, and compared with the data reported previously in the same river. of 16 species of fish examined, the encysted larvae of cyathocotyle orientalis were found most frequently from 9 species of fresh-water fish. the metacercariae of echinochasmus sp., metacercaria hasegawai and metagonimus yokogawai were ...198812811040
[epidemiological studies on metagonimus infection along the hongcheon river, kangwon province]the hongcheon river system flows down through the hongcheon area of kangwon-do, and reaches to the cheongpyeong dam in kyonggi-do. stool specimens from the inhabitants residing along the hongcheon river basin were examined to detect infection rate of metagonimus sp., and the intermediate hosts were collected to detect larval stages. the results obtained were as follows: thirty-nine (33 males and 6 females) out of 529 (314 males and 215 females) inhabitants were infected with metagonimus sp., sho ...198812811049
[epidemiological survey on metagonimus yokogawai infection in the eastern coast area of kangwon-province, korea]an epidemiological survey on metagonimus yokogawai infection was performed in the eastern coast area of kangwon province. formalin-ether concentration technique and stoll's dilution egg counting method were employed to figure out the prevalence and intensity of m. yokogawai infection and eating habits of inhabitants with raw freshwater fish. the results are summarized as follows: the prevalence rate of m. yokogawai infection was 6.6% among a total of 2,357 examinees; 8.6% (115 out of 1,345) in m ...198712886083
[survey on encysted cercaria of trematodes from fresh-water fishes in tongjin riverside areas in korea]in an attempt to clarify the epidemiological feature of distomiasis in tongjin riverside area, the prevalence of distomiasis in the residents and infection rates of the metacercariae in fresh-water fishes were investigated at the upper, middle and lower reaches of the river from january to april 1984. the results obtained were summarized as follows: out of a total of 931 fresh-water fishes which composed of 33 different species, 611 fishes (65.6 %) of 31 species were found positive with digeneti ...198412891012
[epidemiological studies on clonorchis sinensis infection along the nam-river in gyeongnam province, korea]an epidemiological study on clonorchis sinensis infection along the nam-river (total length; 186km) flowing in gyeongsang-nam-do, southern part of korea, was carried out. formalin-ether concentration technique and stoll's egg counting method were employed to figure out the prevalence of c. sinensis infection. for the detection of cercariae from parafossarulus sp., the snail host of c. sinensis, each snail was placed in aerated tap water, and examined for expelled cercariae. for observing the met ...198312902647
[the wormicidal substances of fresh water fishes on clonorchis sinensis: ii. preliminary research on the wormicidal substances from mucous substances of various fresh water fishes]the present work which was investigated in july 1979, was to observe the wormicidal effects of the external mucous substances of 9 species of fresh water fishes (cyprinus carpio, parasilurus asotus, anguilla japonica, ophicephalus argus, carassius carassius; golden crusian carp, misgurnus anguillicaudatus, zacco platypus, pseudorasbora parva and carassius carassius; crusian carp) on cercaria, liberated metacercaria and adult of clonorchis sinensis. the mucous substances extracted by ether from t ...198012902746
[experimental studies on the second intermediate hosts of clonorchis sinensis: iii. observations on the relationship between clavate cells of epidermis and infectivity of metacercariae of clonorchis sinensis in fresh-water fish]in order to know relationship between the clavate cells of epidermis and infectivity of metacercariae of c. sinensis. histological investigation of epidermis was carried out on the 26 species of fresh-water fish collected from the main rivers in korea. results were summarized as it follows: 1. in general clavate cell had a single nucleus at its center. however it was observed in liobagrus mediadiposalis and pelteobagrus fuluidraco that clavate cell had two nuclei with and independent so-called l ...197412913471
[epidemiological studies of clonorchis sinensis prevailed in the peoples of kyungpook province]the author studied the infection rates of clonorchiis sinensis in the inhabitants of kyungpook province, and surveyed infestation rates of clonorchis metacercarias in the twelve kinds of fresh water fishes for the purpose of the eradication. plan of clonorchiasis of kyungpook province. the examination was continued from april 1960 to october 1962 with 24,252 inhabitants ranging from four months to over sixty aged. all the family were tested intradermally with clonorchis antigen and then confirme ...196412913603
phylogenetic structure of zacco platypus (teleostei, cyprinidae) populations on the upper and middle chang jiang (=yangtze) drainage inferred from cytochrome b sequences.we examined the genetic structure and phylogenetic relationships of some chinese populations from the chang jiang (=yangtze) drainage of the cyprinid zacco platypus. we sequenced the complete mitochondrial cytochrome b gene of 64 individuals from 6 upper and middle tributaries of the sichuan and hunan provinces to assess their population structure and systematics. the combined analyses of the phylogenetic information and the population structure suggested that chinese z. platypus consist of four ...200415019619
clonorchis sinensis metacercarial infection in the pond smelt hypomesus olidus and the minnow zacco platypus collected from the soyang and daechung lakes.the pond smelt hypomesus olidus and minnow zacco platypus were collected from the soyang and daechung lakes in january 2003, and their metacercarial infections was examined by the muscle compression and artificial digestion techniques. in the soyang lake, 161 metacercariae of clonorchis sinensis (0.35 per fish) were harvested from 459 pond smelts examined. also, 13 metacercariae of c. sinensis (0.43 per fish), 1 of metagonimus sp., 4 of echinostoma sp., 148 of centrocestus armatus and 44 unident ...200415060339
intron polymorphism (epic-pcr) reveals phylogeographic structure of zacco platypus in china: a possible target for aquaculture development.the present study aims at a phylogeographic description of zacco platypus from southeast china, in order to detect subdivisions within the nominal species. two main basins were sampled: the chang jiang (yangstze river) in central and east china (hunan and sichuan provinces) and the xi jiang, the more southern main tributary of the zhu jiang (pearl river, guangxi province). a total of 27 intron systems were tested, five of them were informative and gave 12 interpretable and polymorphic loci. with ...200515940271
infection status of pond smelts, hypomesus olidus, and other freshwater fishes with trematode metacercariae in 6 large lakes.in order to determine the current infection status of pond smelts, hypomesus olidus, and other freshwater fishes with trematode metacercariae, a total of 4,861 pond smelts and 18 other freshwater fishes, collected from the soyang and uiam lakes in gangwon-do, the unam lake in jeollabuk-do, the jangseong lake in jeollanam-do, the uirim-ji (lake) in chungcheongbuk-do, and the andong lake in gyeongsangbuk-do, were individually digested with 1% pepsin-hcl and examined under a dissecting microscope. ...200616969063
infection status of freshwater fish with metacercariae of clonorchis sinensis in korea.this study investigated freshwater fish for their current infection status with metacercariae of clonorchis sinensis in korea. twenty-one species of freshwater fish (n = 677) were collected from 34 regions nationwidely from february 2007 to june 2008. they were individually examined by digestion technique. eight species of freshwater fish from 17 different regions were recognized positive for the metacercariae of c. sinensis. the positive rates (range of metacercariae number per fish) of fish by ...200819127331
rapid determination of arsenic species in freshwater organisms from the arsenic-rich hayakawa river in japan using hplc-icp-ms.speciation analyses of water-soluble arsenicals from freshwater and biological samples collected from the hayakawa river (kanagawa, japan), which contains a high concentration of arsenic, were performed using high performance liquid chromatography/inductively coupled plasma mass spectrometry (hplc-icp-ms). river water contained only arsenate, which is a pentavalent inorganic arsenical. the water bug stenopsyche marmorata contained inorganic arsenicals accounting for 77% of the water-soluble arse ...200919203781
fishborne trematode metacercariae in freshwater fish from guangxi zhuang autonomous region, china.a survey was performed to investigate the infection status of fishborne trematode (fbt) metacercariae in freshwater fish from guangxi zhuang autonomous region, china. a total of 307 freshwater fish of 31 species were collected from 5 administrative regions of guangxi zhuang autonomous region. they were examined by artificial digestion method from july 2003 to august 2004. no metacercariae were detected in fish from fusui-xian. in fish from mashan-xian and a market in nanning, 3 species of metace ...200919724698
[infection status of freshwater fishes with metacercariae of clonorchis sinensis in liujiang river of guangxi].from march 2008 to march 2009, fishes were caught from the rivers in sanjiang, rongan, rongshui, liucheng, liuzhou and xiangzhou counties, and their metacercarial infections were examined by the muscle compression and digestion techniques. a total of 16,204 freshwater fishes of 35 species were collected. c. sinensis metacercariae were found in 32 species of fishes with an overall infection rate of 10.5% and a mean infection intensity of 4.6 metacercariae per gram. the highest prevalence (21.5%) ...201020666325
[spatial and temporal patterns of stream fish assemblages in the qiupu headwaters national wetland park].identifying and clarifying how stream fish assemblage patterns vary spatially and temporally are basic measures for the conservation and management of fish species. based on data collected from 24 wadeable reaches within the qiupu headwaters national wetland park between may and october 2012, we examined the spatial and temporal patterns of the assemblage structures and diversities, collecting a total of 29 fish species belonging to four orders and ten families. the results of our survey showed ...201323913894
prevalence of zoonotic trematode metacercariae in freshwater fish from gangwon-do, korea.the infection status of zoonotic trematode metacercariae was investigated in a total of 2,293 freshwater fish collected from 11 rivers or streams in 9 administrative regions of gangwon-do, korea for 5 years (2009-2013). all fish were collected by netting methods and examined using the artificial digestion methods. clonorchis sinensis metacercariae were detected in 4 fish species, i.e., pungtungia herzi, squalidus japonicus coreanus, acheilognathus rhombeus, and ladislabia taczanowskii, from only ...201425246719
metagonimus yokogawai: metacercariae survey in fishes and its development to adult worms in various rodents.a parasitological survey for metagonimus yokogawai metacercariae was carried out by examining a total of 321 freshwater fish comprising of 7 species. of the 321 fish samples examined, 182 (56.7%) were found to be infected with m. yokogawai metacercariae. the prevalence of m. yokogawai metacercariae in opsariichthys pachycephalus was 93.4% (86/92), zacco platypus 75.0% (30/40), distoechodon turmirostris 61.3% (38/62), varicorhinus barbatulus 56.5% (13/23), hemibarbus labeo 33.3% (1/3), acrossoche ...201323388732
photic and circadian regulations of melatonin rhythms in fishes.photic and circadian regulations of melatonin rhythms in the pineal organ and the retina of several teleosts were studied to investigate the regulatory mechanisms of melatonin rhythms in fishes. in the eyecup preparations of the goldfish, carassius auratus, both time of day and lighting conditions affected melatonin production, with high melatonin production observed only in the dark-treated group incubated during the 'subjective' night. thus, in the goldfish retina, local photoreceptors and an ...20139500660
benzo(a)pyrene-induced cytochrome p4501a expression of four freshwater fishes (oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus).oryzias latipes, danio rerio, cyprinus carpio, and zacco platypus are useful indicator species for cyp1a biomarker studies; however, comparative studies have not been performed. to compare susceptibility, dose- and time-dependent cyp1a induction at the mrna and protein levels in response to benzo(a)pyrene (bap) exposure was analyzed. at the mrna level, a statistically significant difference was found among the four species; however, such was not observed at the protein level. c. carpio showed th ...201525863331
new frontiers for zebrafish management.the zebrafish (danio rerio) is a preeminent model organism with a wide and expanding utility for numerous scientific disciplines. the same features that once endeared this small freshwater minnow to developmental biologists combined with its relatively high genetic similarity to mammals and the advent of new, more efficient methods for genome editing are now helping to spur expanded growth in its usage in various fields, including toxicology, drug discovery, transplant biology, disease modeling, ...201627443941
intraguild indirect effects through trophic cascades between stream-dwelling fishes.1. to examine the strength and role of indirect effects through trophic cascades by omnivorous fish on algal biomass in streams, we introduced one of four fish species (ayu plecoglossus altivelis altivelis, pike gudgeon pseudogobio esocinus esocinus, japanese dace tribolodon hakonensis and pale chub zacco platypus) in experimental pools. we also investigated the indirect effects of gudgeon, dace and chub on the growth of ayu. 2. we chose the four fish species across a continuum of omnivory. ayu ...200616903054
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