Publications

TitleAbstractYear
Filter
PMID
Filter
mitochondrial genomics of gadine fishes: implications for taxonomy and biogeographic origins from whole-genome data sets.phylogenetic analysis of 13 substantially complete mitochondrial dna genome sequences (14,036 bp) from 10 taxa of gadine codfishes and pollock provides highly corroborated resolution of outstanding questions on their biogeographic evolution. of 6 resolvable nodes among species, 4 were supported by >95% of bootstrap replications in parsimony, distance, likelihood, and similarly high posterior probabilities in bayesian analyses, one by 85%-95% according to the method of analysis, and one by 99% by ...200617110992
radionuclides in marine fishes and birds from amchitka and kiska islands in the aleutians: establishing a baseline.amchitka island (51degrees n lat, 179 degrees e long) was the site of three underground nuclear tests from 1965-1971. there have been no substantive studies of radionuclides in marine fishes and birds in the area since the mid-1970's. in this study, levels of 60co, 52eu, 90sr, 99tc, 129i, 137cs, and the actinides (241am, 238pu, 239,240pu, 234u, 235u, 236u, and 238u) were studied in ten marine fish species (including pacific cod gadus macrocephalus and pacific halibut hippoglossus stenolepis) and ...200717293699
a biomonitoring plan for assessing potential radionuclide exposure using amchitka island in the aleutian chain of alaska as a case study.with the ending of the cold war, the us and other nations were faced with a legacy of nuclear wastes. for some sites where hazardous nuclear wastes will remain in place, methods must be developed to protect human health and the environment. biomonitoring is one method of assessing the status and trends of potential radionuclide exposure from nuclear waste sites, and of providing the public with early warning of any potential harmful exposure. amchitka island (51 degrees n lat, 179 degrees e long ...200717683832
[investigation on the nematode of hysterothylacium aduncum (anisakidae) from bohai sea and yellow sea in china].to investigate hysterothylacium aduncum (anisakidae) infection in marine fishes from bohai sea and yellow sea.200718441985
anisakis simplex larvae: infection status in marine fish and cephalopods purchased from the cooperative fish market in busan, korea.the infection status of marine fish and cephalopods with anisakis simplex third stage larva (l3) was studied over a period of 1 year. a total of 2,537 specimens, which consisted of 40 species of fish and 3 species of cephalopods, were purchased from the cooperative fish market in busan, korea, from august 2006 to july 2007. they were examined for a. simplex l3 from the whole body cavity, viscera, and muscles. a. simplex l3 were confirmed by light microscopy. the overall infection rate reached 34 ...201121461267
molecular analysis of skin bacterial assemblages from codfish and pollock after dry-salted fish production.dry-salted codfish and pollock are commercially important food products with a relatively long shelf life. to date, bacterial assemblages present in these products that are relevant for food safety have been monitored using only classical molecular and/or cultivation methods. the present study employed a rapid and accurate identification method involving pcr with denaturing gradient gel electrophoresis and pyrosequencing to characterize the bacterial assemblages in the skin of three closely rela ...201525951394
cloning and expression analysis of interferon regulatory factor 7 in the pacific cod, gadus macrocephalus.interferon regulatory factor 7 (irf7) plays an important role in regulating the response of type i interferon (ifn) to viral infection. to understand the mechanisms underlying immune reactions in the pacific cod, gadus macrocephalus, the gene encoding g. macrocephalus irf7 was cloned and characterized. the cdna of g. macrocephalus irf7 was also cloned and sequenced. a cdna sequence of 2032 bp was assembled using polymerase chain reaction (pcr) products. it contains an open reading frame of 1323  ...201626702560
transcriptomic analysis and biomarkers (rag1 and igμ) for probing the immune system development in pacific cod, gadus macrocephalus.mortality (>90%) is a big concern in larval rearing facilities of pacific cod, gadus macrocephalus, limiting its culture presently still in the experimental stages. understanding the immune system development of g. macrocephalus is crucial to optimize the aquaculture of this species, to improve the use of economic resources and to avoid abuse of antibiotics. for the transcriptome analysis, using an illumina sequencing platform, 61,775,698 raw reads were acquired. after a de novo assembly, 77,561 ...201525842179
heavy metals in pacific cod (gadus macrocephalus) from the aleutians: location, age, size, and risk.considerable attention has been devoted to the risks from mercury (hg) and polychlorinated biphenyls (pcb) to high-level predators (including humans) who consume freshwater fish. although the u.s. food and drug administration (fda) issued advisories because of hg for four marine fish species, there are few data on lead (pb), cadmium (cd), or other metals in bering sea fish generally, or on the risk these levels pose to the fish themselves or to consumers of marine fish. levels of arsenic (as), c ...200717966061
oscillating trophic control induces community reorganization in a marine ecosystem.understanding how climate regulates trophic control may help to elucidate the causes of transitions between alternate ecosystem states following climate regime shifts. we used a 34-year time series of the abundance of pacific cod (gadus macrocephalus) and five prey species to show that the nature of trophic control in a north pacific ecosystem depends on climate state. rapid warming in the 1970s caused an oscillation between bottom-up and top-down control. this shift to top-down control apparent ...200717883409
risk to consumers from mercury in pacific cod (gadus macrocephalus) from the aleutians: fish age and size effects.while there has been considerable attention devoted to the risks to high level consumers from mercury in freshwater fish, relatively little attention has been devoted to saltwater fish. although the u.s. food and drug administration has issued advisories based on mercury for four saltwater species or groups of fish, there are few data on mercury levels generally, or on the risk these levels pose to the fish themselves or to consumers of marine fish. we examined total mercury levels in liver and ...200717599825
identification of gadoid species (pisces, gadidae) by pcr-rflp analysis.a rapid pcr-rflp analysis was optimized to identify the presence of 3 closely related gadoid fish species: alaska pollack theragra chalcogramma, pacific cod gadus macrocephalus and atlantic cod gadus morhua in commercial seafood products. gadoid universal primers were designed for pcr amplification of a 558-bp fragment encoding the mitochondrial cytochrome b gene. without purification of the pcr products, double digestion with eco32i and eco105i restriction enzymes generated reproducible species ...200515741666
miltpain, a cysteine proteinase, from milt of pacific cod (gadus macrocephalus): purification and characterization.miltpain (ec.3.4.22.-) is a cysteine proteinase that preferentially hydrolyzes basic proteins, previously found in the milt of chum salmon. here we report a similar cysteine proteinase in the milt of the marine pacific cod. the enzyme was isolated and purified 6900-fold and with an estimated mass of 63 kda by gel filtration chromatography and 72 kda by sds/page. cod miltpain has an optimum ph of 6.0 for z-arg-arg-mca hydrolysis, and km of 11.5 microm and kcat of 19.0 s-1 with z-arg-arg-mca. it r ...200010904866
description of electrophoretic loci in atlantic cod, gadus morhua, and comparison with pacific cod, gadus macrocephalus. 19883372261
oxidative deterioration of flesh lipids of pacific cod (gadus macrocephalus). 19676039776
evidence for and characterization of nervous necrosis virus infection in pacific cod (gadus macrocephalus).a mortality rate higher than 90% was observed in a larva-rearing facility for pacific cod, gadus macrocephalus, in china. larvae showing clinical signs of infection were collected. initial suspicion of nervous necrosis virus (nnv) infection was confirmed by sequencing, absolute quantification real-time pcr (a-qpcr), and electron microscopy. the nucleotide sequence of rna2 was 1,375 bases long (genbank no. km576685), coding for a single orf corresponding to the capsid protein from residues 21 to ...201526115691
free radical scavenging and angiotensin-i converting enzyme inhibitory peptides from pacific cod (gadus macrocephalus) skin gelatin.potent antioxidative peptides were purified from pacific cod (gadus macrocephalus) skin gelatin using alcalase, neutrase, papain, trypsin, pepsin, and α-chymotrypsin. among them, the papain hydrolysate exhibited the highest antioxidant activity. therefore, it was further purified and obtained two peptides with amino acid sequences of thr-cys-ser-pro (388 da) and thr-gly-gly-gly-asn-val (485.5 da). the antioxidant activity of the purified peptides was performed by electron spin resonance techniqu ...201121945677
characterization of the capsid protein gene from a nodavirus strain affecting the atlantic halibut hippoglossus hippoglossus and design of an optimal reverse-transcriptase polymerase chain reaction (rt-pcr) detection assay.a 1349 nucleotide fragment of the rna2 from a nodavirus affecting atlantic halibut hippoglossus hippoglossus was characterised and the nuclotide sequence (accession no. aj245641) was employed to develop an optimal reverse-transcriptase polymerase chain reaction (rt-pcr) detection assay. the sequenced part of the rna2 of atlantic halibut nodavirus (strain ah95nora) was highly similar in organisation to that of the rna2 of striped jack nervous necrosis virus (sjnnv), and comprised features common ...200010715813
successful aquatic animal disease emergency programmes.the authors provide examples of emergency programmes which have been successful in eradicating or controlling certain diseases of aquatic animals. the paper is divided into four parts. the first part describes the initial isolation of viral haemorrhagic septicaemia (vhs) virus in north america in the autumn of 1988 from feral adult chinook (oncorhynchus tshawytscha) and coho salmon (o. kisutch) returning for spawning. the fish disease control policies at both state and federal levels in the unit ...199910190216
organochlorine contaminants in fishes from coastal waters west of amukta pass, aleutian islands, alaska, usa.organochlorines were examined in liver and stable isotopes in muscle of fishes from the western aleutian islands, alaska, in relation to islands or locations affected by military occupation. pacific cod (gadus macrocephalus), pacific halibut (hippoglossus stenolepis), and rock greenling (hexagrammos lagocephalus) were collected from nearshore waters at contemporary (decommissioned) and historical (world war ii) military locations, as well as at reference locations. total (sigma) polychlorinated ...200919374473
[detection of pacific cod and capelin roes in alaska pollack roe product by real-time pcr assay].a rapid and sensitive taqman real-time pcr assay for the detection of pacific cod (gadus macrocephalus) and capelin (mallotus villosus) roes in alaska pollack (theragra chalcogramma) roe product was developed. the primers and the taqman mgb (minor groove binder) probes were designed based on the gene encoding cytochrome b for the specific detection of alaska pollack, pacific cod and capelin. this real-time pcr assay had the detection limit of 0.002 ng/microl mitochondrial dna and showed no cross ...201020595791
multiple ice-age refugia in pacific cod, gadus macrocephalus.pleistocene ice-ages greatly influenced the historical abundances of pacific cod, gadus macrocephalus, in the north pacific and its marginal seas. we surveyed genetic variation at 11 microsatellite loci and mitochondrial (mt) dna in samples from twelve locations from the sea of japan to washington state. both microsatellite (mean h = 0.868) and mtdna haplotype (mean h = 0.958) diversities were large and did not show any geographical trends. genetic differentiation between samples was significant ...201020819160
remarkably low mtdna control-region diversity and shallow population structure in pacific cod gadus macrocephalus.to investigate the genetic diversity and describe the population structure in gadus macrocephalus, a 452 base pair (bp) fragment of the mitochondrial dna control region was analysed in 259 individuals. the results showed remarkably low nucleotide diversity and a lack of genealogical structure. small but significant genetic differentiations, however, were detected among north-western pacific populations, but no large-scale regional differences were detected. these results indicate that population ...201021039491
simple preparation of pacific cod trypsin for enzymatic peptide synthesis.trypsin from the pyloric caeca of pacific cod (gadus macrocephalus) was easily prepared by affinity chromatography on benzamidine sepharose 6b and gel filtration on superdex 75. pacific cod trypsin was composed of three isozymes, and their molecular masses were estimated 23,756.34 da, 23,939.62 da, and 24,114.81 da by desorption/ionization time-of-flight mass spectroscopy (maldi/tof-ms) and their isoelectric points (pis) were approximately 5.1, 6.0, and 6.2, respectively. the isolated pacific co ...201122312475
diel vertical migration of adult pacific cod gadus macrocephalus in alaska.the diel vertical migration (dvm) of pacific cod gadus macrocephalus was examined using depth and temperature data from 250 recaptured archival tags deployed on g. macrocephalus in the eastern bering sea and in the gulf of alaska near kodiak island. dvm of two types, deeper during daytime (type i) and deeper during night-time (type ii), occurred frequently (15-40% of all days) in g. macrocephalus released at all sites. most individuals displayed both diel types, with each type of behaviour lasti ...201323808699
temperature-mediated survival, development and hatching variation of pacific cod gadus macrocephalus eggs.laboratory-validated data on the survival, development and hatching responses of fertilized pacific cod gadus macrocephalus eggs from the northern japan stock were determined through an incubation experiment. the optimum temperature for survival until hatching ranged from 4 to 8°c. no significant difference in development rates was found between the populations from mutsu bay, japan, and western canadian coastal waters even though the samples may belong to different g. macrocephalus stocks. gadu ...201424344879
envelope surface ultrastructure and specific gravity of artificially fertilized pacific cod gadus macrocephalus eggs.the envelope surface ultrastructure and specific gravity of artificially fertilized eggs of the pacific cod gadus macrocephalus were examined. the unfertilized, demersal and slightly adhesive eggs of g. macrocephalus were almost spherical and had no oil globules. wrinkled envelope surface with elaborated hexagonal reticulated patterns and type i micropyle were observed under a scanning electron microscope. the adhesiveness of the eggs was lost at the blastodermal-cap stage after fertilization. t ...201424417391
[formation of genetic diversity in populations of pacific cod (gadus macrocephalus tilesius) (gadidae)].the variability of the gmo3, gmo34, gmo35, pgmo32, gmo19 microsatellite dna loci was examined in the population samples of pacific cod from different parts of the north pacific. the statistical tests showed that the differentiation of the population was the result of different levels of reproductive autonomy of the individual pacific cod populations, formed in the pleistocene-holocene period with the specific features of transgressions and regressions of the world ocean, and supported by the exi ...201325470931
[formation of genetic diversity in populations of pacific cod (gadus macrocephalus tilesius) (gadidae)].the variability of the gmo3, gmo34, gmo35, pgmo32, gmo19 microsatellite dna loci was examined in the population samples of pacific cod from different parts of the north pacific. the statistical tests showed that the differentiation of the population was the result of different levels of reproductive autonomy of the individual pacific cod populations, formed in the pleistocene-holocene period with the specific features of transgressions and regressions of the world ocean, and supported by the exi ...201325508558
genetic differentiation of pacific cod gadus macrocephalus in the sea of okhotsk and in the bering sea.genetic differentiation of the pacific cod gadus macrocephalus was studied. samples from six regions of the sea of okhotsk and the bering sea were analyzed with two mtdna genetic markers-gene of cytochrome 1 and the control region (d-loop). comparative analysis showed significant genetic differentiation between the two groups of samples. the first group included samples from tauiskaya bay and waters of western kamchatka. the second group consisted of the samples collected in the waters of the it ...201526728732
transcriptomic features associated with energy production in the muscles of pacific bluefin tuna and pacific cod.bluefin tuna are high-performance swimmers and top predators in the open ocean. their swimming is grounded by unique features including an exceptional glycolytic potential in white muscle, which is supported by high enzymatic activities. here we performed high-throughput rna sequencing (rna-seq) in muscles of the pacific bluefin tuna (thunnus orientalis) and pacific cod (gadus macrocephalus) and conducted a comparative transcriptomic analysis of genes related to energy production. we found that ...201626924100
restricted gene flow for gadus macrocephalus from yellow sea based on microsatellite markers: geographic block of tsushima current.the pacific cod gadus macrocephalus is a demersal, economically important fish in the family gadidae. population genetic differentiation of pacific cod was examined across its northwestern pacific range by screening variation of eight microsatellite loci in the present study. all four populations exhibited high genetic diversity. pairwise fixation index (fst) suggested a moderate to high level of genetic differentiation among populations. population of the yellow sea (ys) showed higher genetic d ...201627043534
protective effect of cod (gadus macrocephalus) skin collagen peptides on acetic acid-induced gastric ulcer in rats.this research was performed to explore the protective effect of cod skin collagen peptides (ccp) on gastric ulcer induced by acetic acid. the ccp were fractionated into low molecular ccp (lmccp, mw < 3 kda) and high molecular ccp (hmccp, mw > 3 kda). in hmccp and lmccp, glycine of accounted for about one-third of the total amino acids without cysteine and tryptophan, and hydrophobic amino acids accounted for about 50%. after 21 d ccp treatment (60 or 300 mg/kg, p.o./daily), the healing effects o ...201627219644
protective effect of gelatin polypeptides from pacific cod (gadus macrocephalus) against uv irradiation-induced damages by inhibiting inflammation and improving transforming growth factor-β/smad signaling pathway.exposure to ultraviolet (uv) radiation results in skin damage, collagen reduction in the dermis, and consequently, premature skin aging (photoaging). the goal of this study was to examine the effect of gelatin hydrolysate (ch) from pacific cod (gadus macrocephalus) skin on uv irradiation-induced inflammation and collagen reduction of photoaging mouse skin. the effect of ch on the activities of endogenous antioxidant enzymes was investigated. the expressions of nuclear factor-κb (nf-κb), proinfla ...201627491029
genetic variation and local differences in pacific cod gadus macrocephalus around japan.the population structure of the pacific cod gadus macrocephalus was examined using 15 microsatellite loci and mitochondrial dna (nd2 region). in total, 274 individuals were sampled from 16 locations around japan to estimate the level of genetic differentiation and effective population size (ne ). pairwise fst , analysis of molecular variance and bayesian clustering analysis suggested the presence of two genetically distinct groups in waters around japan, with a higher ne value in the eastern gro ...201727723107
effect of calcium-binding peptide from pacific cod (gadus macrocephalus) bone on calcium bioavailability in rats.bone collagen peptide with high affinity to ca was extracted from pacific cod (gadus macrocephalus) bone. ftir spectra of calcium-binding bone collagen peptide showed that band at 3381cm(-1) shifted to 3361cm(-1), 1455cm(-1) moved to 1411cm(-1), and amide ii became deeper valley, compared with that of bone collagen peptide. this peptide was sequenced by q-tof-ms and sequences of gly-pro-glu-gly, gly-glu-lys, gly-pro-leu-gly and gly-leu-pro-gly appeared repeatedly in some peptides. from sem, afte ...201727979216
mercury in tunas and blue marlin in the north pacific ocean.models and data from the north pacific ocean indicate that mercury concentrations in water and biota are increasing in response to (global or hemispheric) anthropogenic mercury releases. in the present study, we provide an updated record of mercury in yellowfin tuna (thunnus albacares) caught near hawaii that confirms an earlier conclusion that mercury concentrations in these fish are increasing at a rate similar to that observed in waters shallower than 1000 m. we also compiled and reanalyzed d ...201728264147
Displaying items 1 - 37 of 37