hybridization among cryptic species of the cellar fungus coniophora puteana (basidiomycota).in this study we have analysed the genetic variation and phylogeography in a global sample of the cellar fungus coniophora puteana, which is an important destroyer of wooden materials indoor. multilocus genealogies of three dna regions (beta tubulin, nrdna its and translation elongation factor 1alpha) revealed the occurrence of three cryptic species (ps1-3) in the morphotaxon c. puteana. one of the lineages (ps3) is apparently restricted to north america while the other two (ps1-2) have wider di ...200717217352
characterisation of the initial degradation stage of scots pine (pinus sylvestris l.) sapwood after attack by brown-rot fungus coniophora our study, early period degradation (10 days) of scots pine (pinus sylvestris l.) sapwood by the brown-rot fungus coniophora puteana (schum.: fr.) karst. (bam ebw.15) was followed at the wood chemical composition and ultrastructure-level, and highlighted the generation of reactive oxygen species (ros). an advanced decay period of 50 days was chosen for comparison of the degradation dynamics. scanning uv microspectrophotometry (umsp) analyses of lignin distribution in wood cells revealed that ...201121327804
improved molecular methods to characterise serpula lacrymans and other basidiomycetes involved in wood decay.early detection of indoor wood-decay fungi is crucial to prevent building deterioration and thereby avoid considerable economic loss. due to their increased sensitivity, two reliable dna-based fingerprinting techniques, capillary electrophoresis single-strand conformation polymorphism (ce-sscp) and denaturing high-performance liquid chromatography (dhplc), were used to identify serpula lacrymans and to profile wood-rot basidiomycetes in the built environment. molecular fungal diversity was asses ...201021146565
detection of hydroxyl radicals produced by wood-decomposing fungi.the hydroxyl radical ((*)oh) is believed to act as the small non-enzymatic agent involved in the brown-rot decay of wood. however, knowledge about the relation between hydroxyl radical production and the activity of wood-decomposing fungi in wood or about the significance of these radicals during interactions with other organisms is limited due to a lack of reliable methods for detecting the radicals. a sensitive and specific fluorescence method was developed in this study to detect the producti ...200219709206
effects of leaching on fungal growth and decay of western redcedar.we tested the effect of leaching on the concentration of western red cedar (wrc; thuja plicata donn ex d. don) heartwood extractives that are known to exhibit antimicrobial activity and correlated this with fungal growth and decay. we assessed the extractive tolerance of the following fungal species: acanthophysium lividocaeruleum, coniophora puteana, heterobasidion annosum, pachnocybe ferruginea, phellinus sulphurascens, and phellinus weirii by measuring their growth rate (mm/day) on media with ...200919483786
decay resistance of wood treated with boric acid and tall oil this study, the effect of two boric acid concentrations (1% and 2%) and four derivates of tall oil with varying chemical composition were tested separately and in combination. the tall oil derivates were chosen in a way that they consist of different amounts of free fatty, resin acids and neutral compounds. decay tests using two brown rot fungi (postia placenta and coniophora puteana) were performed on both unleached and leached test samples. boric acid showed a low weight loss in test sample ...200817931858
fourier-based spatial mapping of oscillatory phenomena in fungi.microorganisms display a range of oscillatory phenomena that operate over different temporal scales. fourier analysis provides a compact description of such oscillations in terms of their frequency, magnitude and phase. however, in the majority of studies there is no explicit consideration of the spatial organisation of the oscillation. here we describe procedures and a software package to map oscillatory phenomena in microorganisms in both the time and frequency domains. key parameters of inter ...200717434770
degradation of wood preservatives by fungi.wood-inhabiting fungi, not necessarily responsible for major decay, are shown to be capable of degrading a toxic compound into a less potent form, thus rendering it less effective in protecting wood from decay by less-tolerant basidiomycetous wood-destroyers. sweetgum or pine sapwood blocks treated with preservatives (ammoniacal copper arsenate, fluor-chrome-arsenate-dinitrophenol, a creosote or pentachlorophenol) were exposed progressively to two different wood-inhabiting fungi with sterilizati ...196416349644
characterization and molecular cloning of cellobiose dehydrogenase from the brown-rot fungus coniophora puteana.cellobiose dehydrogenase (cdh) was purified from the brown-rot fungus coniophora puteana grown in culture containing crystalline cellulose as a carbon source. the purified enzyme gave a single band at 115 kda on sds-page and showed a typical flavocytochrome absorption spectrum. the enzyme oxidized both cellobiose and cellooligosaccharides, but not their monomer, glucose, suggesting typical kinetic features of cdh. a cdna encoding cdh was cloned by rt-pcr using primers designed from the consensus ...200416233666
significance of wood terpenoids in the resistance of scots pine provenances against the old house borer, hylotrupes bajulus, and brown-rot fungus, coniophora puteana.we tested how terpenoid (i.e., monoterpenes and resin acids) composition and concentration in wood affects resistance against wood-borers and decaying fungi. scots pine (pinus sylvestris) wood from nine provenances having variable terpenoid profiles was studied against the old house borer, hylotrupes bajulus, and the decay fungus, coniophora puteana. provenances represented a 1200-km n-s transect from estonia to northern finland, but they were all cultivated for 7 years in the same nursery field ...200415074661
insecticidal and fungicidal compounds from isatis tinctoria.tryptanthrin (1), indole-3-acetonitrile (2) and p-coumaric acid methylester (3) were isolated from the aerial parts of isatis tinctoria l. the compounds show insecticidal and anti-feedant activity against termites (reticulitermis santonensis), insect preventive and control activity against larvae of the house longhorn beetle (hylotrupes bajulus) and fungicidal activity against the brown-rot fungus (coniophora puteana).19948148009
validity of detection of microbial growth in buildings by trained dogs.microbial growth in buildings may evoke respiratory and other symptoms in the occupants and promote decay of construction materials. the decay in wood is usually caused by dry-rot fungus, leading to the decomposition of cellulose and lignin. there are also some mold fungi and bacteria that can use wood as a nutrient. in this study, two trained dogs were used to detect microbial growth present in buildings. the rot fungi serpula lacrymans, coniophora puteana and antrodia sinuosa were used in the ...200212222611
determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity.beech and pine wood blocks were treated with 1,3-dimethylol-4,5-dihydroxyethylen urea (dmdheu) to increasing weight percent gains (wpg). the resistance of the treated specimens against trametes versicolor and coniophora puteana, determined as mass loss, increased with increasing wpg of dmdheu. metabolic activity of the fungi in the wood blocks was assessed as total esterase activity (tea) based on the hydrolysis of fluorescein diacetate and as heat or energy production determined by isothermal m ...200818542949
identification by gas chromatography-mass spectrometry of the volatile organic compounds emitted from the wood-rotting fungi serpula lacrymans and coniophora puteana, and from pinus sylvestris timber.volatile organic compounds (vocs) emitted by two wood-rotting basidiomycete fungi, serpula lacrymans (dry rot fungus) and coniophora puteana (cellar fungus), and the timber of pinus sylvestris (scots pine), were identified. several volatile collection techniques were employed including dichloromethane solvent extraction, solid-phase microextraction (spme) and thermal desorption of vocs entrained on tenax gr. in addition, a new method of solid sample injection (ssi) is described which utilises a ...200415446714
isolation and characterization of the cellobiose dehydrogenase from the brown-rot fungus coniophora puteana (schum ex fr.) karst.the cellobiose dehydrogenase secreted by coniophora puteana (schum ex fr) karsten during growth on cellulose was isolated by successive anion-exchange chromatography on q sepharose fast flow and on tsk deae-650s and gel filtration on superose 12. the enzyme was recovered at a 41% yield with a 43-fold increase in specific activity. the purified sample was homogeneous by polyacrylamide gel electrophoresis (page), sodium lauryl sulfate (sds)-page, and electrophoretic titration curve analysis and st ...19938434937
purification and characterization of two exo-cellobiohydrolases from the brown-rot fungus coniophora puteana (schum ex fr) karst.two extracellular exo-cellobiohydrolases (ec were purified to homogeneity from the culture filtrate of the brown-rot fungus coniophora puteana (schum ex fr) karsten, strain empa 62. the purification scheme involved three successive chromatographic steps, namely q sepharose fast flow, superose 12, and fractogel tsk deae-650s. the two enzymes, named cellobiohydrolase (cbh) i and cbh ii, were purified by a factor of 4.6 and 3.9, respectively, with an activity recovery of 9 and 19% of tota ...19938434936
fungicidal value of wood tar from pyrolysis of treated wood.the objective of the paper was to estimate the fungicidal value of wood tar extracted as a product of pyrolysis of wood previously treated with either creosote oil or ccb-type salt preservative. the effectiveness of wood treated with one of these two wood tar residuals was compared to the effectiveness of wood treated with virgin creosote oil (type wei-b) and an untreated control. wood was impregnated with alcohol solutions of the two extracted preservatives or virgin creosote oil and then subje ...200717011772
evaluation of lactic acid bacterium from chilli waste as a potential antifungal agent for wood products.aims:  the aim of this study was to isolate lactic acid bacteria from chilli waste and evaluate metabolites produced for the ability to arrest wood decay. methods and results:  using an optical density screening method, one bacterium (isolate c11) was identified as having pronounced antifungal properties against oligoporus placenta. this isolate was identified as lactobacillus brevis by 16s rrna gene sequencing. to determine antifungal activity in wood, pinus radiata blocks were impregnated with ...201222236054
chemical composition and natural durability of juvenile and mature heartwood of robinia pseudoacacia l.the aim of this study was to characterize the properties of juvenile and mature heartwood of robinia pseudoacacia l. (black locust). the content, the composition, and subcellular localization of heartwood extractives were studied in 14 old-grown trees from forest sites in germany and hungary, as well as in 16 younger trees of four clone types. heartwood extractives (methanol and acetone extraction) were analysed by hplc-chromatography. uv microspectrophotometry was used to localize the extractiv ...201121779654
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