| bacillus krulwichiae sp. nov., a halotolerant obligate alkaliphile that utilizes benzoate and m-hydroxybenzoate. | obligate alkaliphilic strains, am31d(t) and am11d, that utilize benzoate and m-hydroxybenzoate were isolated from soil obtained from tsukuba, ibaraki, japan. the isolates grew at ph 8-10, but not at neutral ph. they were gram-positive, facultatively anaerobic, straight rods with peritrichous flagella and produced ellipsoidal spores. the isolates reduced nitrate to nitrite and grew in 0-14 % nacl, but not in higher concentrations. the major isoprenoid quinones were menaquinone-5, -6 and -7, and t ... | 2003 | 13130043 |
| bacillus trypoxylicola sp. nov., xylanase-producing alkaliphilic bacteria isolated from the guts of japanese horned beetle larvae (trypoxylus dichotomus septentrionalis). | three xylanase-producing alkaliphilic strains, su1(t), 36ac4 and 36ac6, were isolated from the guts of larvae of the japanese horned beetle (trypoxylus dichotomus septentrionalis). the isolates stained gram-positive and were aerobic, spore-forming, non-motile and rod-shaped and grew optimally at 30 degrees c and ph 9. they contained mk-7 as the major isoprenoid quinone and iso-c(15 : 0), anteiso-c(15 : 0), anteiso-c(17 : 0) and iso-c(17 : 0) as the major fatty acids. the dna g+c contents of the ... | 2010 | 19648346 |
| purification and properties of a new thermostable cyclodextrin glucanotransferase from bacillus pseudalcaliphilus 8sb. | a new cyclodextrin glucanotransferase (cgtase, ec 2.4.1.19) from an alkaliphilic halotolerant bacillus pseudalcaliphilus 8sb was studied in respect to its γ-cyclizing activity. an efficient conversion of a raw corn starch into only two types of cyclodextrins (β- and γ-cd) was achieved by the purified enzyme. crude enzyme obtained by ultrafiltration was purified up to fivefold by starch adsorption with a recovery of 62% activity. the enzyme was a monomer with a molecular mass 71 kda estimated by ... | 2011 | 21894524 |
| high-level secretion and characterization of cyclodextrin glycosyltransferase in recombinant komagataella phaffii. | cyclodextrin glycosyltransferase (cgtase) catalyzes the conversion of starch into cyclodextrin (cd), which is widely applied in food, pharmaceutical, cosmetic, and agricultural industries. for efficient production of cd, high yield of cgtase with good characteristics is necessary. in this study, the cgt gene from bacillus pseudalcaliphilus was expressed in komagataella phaffii after codon optimization and expression vector selection. the β-cgtase activity in the transformant reached 3885.1uml-1, ... | 2017 | 28757288 |
| genetic and rat toxicity studies of cyclodextrin glucanotransferase. | microbiologically derived cyclodextrin glucanotransferase (cgtase) is used commercially as a processing agent in manufacture of food, pharmaceuticals, and cosmetics. its toxic potential was evaluated in anticipation of use in the production of alpha-glycosyl isoquercitrin, a water-soluble form of quercetin. | 2016 | 28959560 |
| draft genome sequence of bacillus pseudalcaliphilus pn-137t (dsm 8725), an alkaliphilic halotolerant bacterium isolated from garden soils. | bacillus pseudalcaliphilus pn-137(t) (dsm 8725) is a gram-positive, spore-forming, alkaliphilic, and halotolerant bacterium. here, we report the 4.49-mb genome sequence of b. pseudalcaliphilus pn-137(t), which will accelerate the application of this alkaliphile and provide useful information for genomic taxonomy and phylogenomics of bacillus-like bacteria. | 2015 | 26272579 |