| bacillus lehensis sp. nov., an alkalitolerant bacterium isolated from soil. | a gram-positive, endospore-forming, alkalitolerant bacterial strain, designated mlb2t, was isolated from soil from leh, india, and was subjected to a polyphasic taxonomic study. the strain exhibited phenotypic properties that included chemotaxonomic characteristics consistent with its classification in the genus bacillus. growth was observed at ph 7.0-11.0, but not at ph 6.0. the dna g+c content was 41.4 mol%. the highest level of 16s rrna gene sequence similarity was with bacillus oshimensis jc ... | 2007 | 17267957 |
| bacillus plakortidis sp. nov. and bacillus murimartini sp. nov., novel alkalitolerant members of rrna group 6. | the gram-positive, alkali- and salt-tolerant marine bacterium strain p203(t) is described together with its closest phylogenetic neighbour, terrestrial isolate lmg 21005(t). strain p203(t) was isolated from material from the sponge plakortis simplex that was obtained from the sula-ridge, norwegian sea. strain lmg 21005(t) was an undescribed strain that was isolated from a church wall mural in germany. strains p203(t) and lmg 21005(t) were identified as novel alkalitolerant members of the bacillu ... | 2007 | 18048744 |
| structural differences in gut bacteria communities in developmental stages of natural populations of lutzomyia evansi from colombia's caribbean coast. | lutzomyia evansi, a phlebotomine insect endemic to colombia's caribbean coast, is considered to be the main vector of visceral and cutaneous leishmaniasis in the region. although insects of this species can harbor pathogenic and non-pathogenic microorganisms in their intestinal microbiota, there is little information available about the diversity of gut bacteria present in lutzomyia evansi. in this study, conventional microbiological methods and molecular tools were used to assess the compositio ... | 2016 | 27618991 |
| effects of ensiling fermentation and aerobic deterioration on the bacterial community in italian ryegrass, guinea grass, and whole-crop maize silages stored at high moisture content. | the effects of storage period and aerobic deterioration on the bacterial community were examined in italian ryegrass (ir), guinea grass (gg), and whole-crop maize (wm) silages. direct-cut forages were stored in a laboratory silo for 3, 7, 14, 28, 56, and 120 d without any additives; live counts, content of fermentation products, and characteristics of the bacterial community were determined. 2,3-butanediol, acetic acid, and lactic acid were the dominant fermentation products in the ir, gg, and w ... | 2013 | 25049913 |
| secretory expression, functional characterization, and molecular genetic analysis of novel halo-solvent-tolerant protease from bacillus gibsonii. | a novel protease gene from bacillus gibsonii, aprbg, was cloned, expressed in b. subtilis, and characterized. high-level expression of aprbg was achieved in the recombinant strain when a junction was present between the promoter and the target gene. the purified recombinant enzyme exhibited similar n-terminal sequences and catalytic properties to the native enzyme, including high affinity and hydrolytic efficiency toward various substrates and a superior performance when exposed to various metal ... | 2014 | 24150493 |
| surface charge engineering of a bacillus gibsonii subtilisin protease. | in proteins, a posttranslational deamidation process converts asparagine (asn) and glutamine (gln) residues into negatively charged aspartic (asp) and glutamic acid (glu), respectively. this process changes the protein net charge affecting enzyme activity, ph optimum, and stability. understanding the principles which affect these enzyme properties would be valuable for protein engineering in general. in this work, three criteria for selecting amino acid substitutions of the deamidation type in t ... | 2013 | 23179617 |
| increasing activity and thermal resistance of bacillus gibsonii alkaline protease (bgap) by directed evolution. | bacillus gibsonii alkaline protease (bgap) is a recently reported subtilisin protease exhibiting activity and stability properties suitable for applications in laundry and dish washing detergents. however, bgap suffers from a significant decrease of activity at low temperatures. in order to increase bgap activity at 15°c, a directed evolution campaign based on the sesam random mutagenesis method was performed. an optimized microtiter plate expression system in b. subtilis was established and cla ... | 2013 | 23097081 |
| biosorption characteristics of bacillus gibsonii s-2 waste biomass for removal of lead (ii) from aqueous solution. | lead (ii) has been as one of the most toxic heavy metals because it is associated with many health hazards. therefore, people are increasingly interested in discovering new methods for effectively and economically scavenging lead (ii) from the aquatic system. recent studies demonstrate biosorption is a promising technology for the treatment of pollutant streams. to apply these techniques, suitable adsorbents with high efficiency and low cost are demanded. the waste biomass of bacillus gibsonii s ... | 2013 | 22961488 |