| characterization of spirochetes isolated from ticks (ixodes tanuki, ixodes turdus, and ixodes columnae) and comparison of the sequences with those of borrelia burgdorferi sensu lato strains. | ixodes persulcatus serves as a tick vector for borrelia garinii and borrelia afzelii in japan; however, unidentified spirochetes have been isolated from other species of ticks. in this study, 13 isolates from ticks (6 from ixodes tanuki, 6 from ixodes turdus, and 1 from ixodes columnae) and 3 isolates from voles (clethrionomys rufocanus) were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, rrna gene restriction fragment length polymorphism, partial sequencing of the o ... | 1996 | 8779571 |
| borrelia tanukii sp. nov. and borrelia turdae sp. nov. found from ixodid ticks in japan: rapid species identification by 16s rrna gene-targeted pcr analysis. | based on the results of rflp-ribotyping, whole dna/dna hybridization and phylogenetic analysis of the 16s rrna gene, we previously defined two genomic groups of spirochetes closely related to borrelia burgdorferi sensu lato: group hk501 for strains isolated from ixodes tanuki ticks and group ya501 for strains isolated from ixodes turdus ticks. in this report, we propose that group hk501 should be classified as borrelia tanukii sp. nov. and group ya501 as borrelia turdae sp. nov. the alignment of ... | 1996 | 8985944 |
| analysis of the organization of multicopy linear- and circular-plasmid-carried open reading frames in borrelia burgdorferi sensu lato isolates. | plasmid cp8.3 of borrelia afzelii ip21 carries several open reading frames (orfs) and a 184-bp inverted repeat (ir) element. it has been speculated that this plasmid may encode factors involved in virulence or infectivity. in this report, we have characterized the distribution, molecular variability, and organization of orfs 1, 2, and 4 and the ir elements among isolates of the borrelia burgdorferi sensu lato complex. orfs 1 and 2 are contained within a segment of cp8.3 that is bordered by the i ... | 1998 | 9488408 |
| expanded diversity among californian borrelia isolates and description of borrelia bissettii sp. nov. (formerly borrelia group dn127). | up to now, the only species in the complex borrelia burgdorferi sensu lato known to cause lyme borreliosis in the united states has been b. burgdorferi sensu stricto. however, some atypical strains closely related to the previously designated genomic group dn127 have been isolated in the united states, mostly in california. to explore the diversity of b. burgdorferi sensu lato group dn127, we analyzed the nucleotide sequences of the rrf-rrl intergenic spacer regions from 19 atypical strains (18 ... | 1998 | 9817861 |
| phenotypic and genetic characterization of a novel borrelia burgdorferi sensu lato isolate from a patient with lyme borreliosis. | borrelia burgdorferi sensu lato a14s was cultured from a skin biopsy specimen of a patient with erythema migrans in the netherlands. this isolate had a unique dna fingerprint pattern compared to 135 other b. burgdorferi sensu lato isolates. in this study, the isolate a14s was further characterized by protein analysis with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and reactivity with various monoclonal antibodies. in addition, the 16s rrna, ospa, and ospc genes, as well ... | 1999 | 10449497 |
| molecular typing of borrelia burgdorferi sensu lato: taxonomic, epidemiological, and clinical implications. | borrelia burgdorferi sensu lato, the spirochete that causes human lyme borreliosis (lb), is a genetically and phenotypically divergent species. in the past several years, various molecular approaches have been developed and used to determine the phenotypic and genetic heterogeneity within the lb-related spirochetes and their potential association with distinct clinical syndromes. these methods include serotyping, multilocus enzyme electrophoresis, dna-dna reassociation analysis, rrna gene restri ... | 1999 | 10515907 |
| prevalence of lyme disease borrelia spp. in ticks from migratory birds on the japanese mainland. | borrelia sp. prevalence in ticks on migratory birds was surveyed in central japan. in autumn, a total of 1,733 birds representing 40 species were examined for ticks. a total of 361 ticks were obtained from 173 birds of 15 species, and these ticks were immature haemaphysalis flava (94.4%), haemaphysalis longicornis, ixodes columnae, ixodes persulcatus, ixodes turdus, and an unidentified ixodes species. of these, 27 juveniles of h. flava on turdus pallidus, turdus cardis, or emberiza spodocephala, ... | 2000 | 10698761 |
| differentiation of borrelia burgdorferi sensu lato on the basis of rna polymerase gene (rpob) sequences. | we determined the nucleotide sequences (329 bp) of the rpob dnas from 22 reference strains of borrelia. no insertions or deletions were observed. deduced amino acid sequences of amplified rpob dna comprised 109 amino acid residues (n(450) to m(558) [escherichia coli numbering]). all amino acid sequences were identical with the exception of those of borrelia lusitaniae potib2 (t(461)-->a) and b. bissettii dn127 (i(498)-->v). each species of b. burgdorferi sensu lato was differentiated as a distin ... | 2000 | 10878043 |
| tick-borne rickettsial pathogens in ticks and small mammals in korea. | in order to investigate the prevalence of tick-borne infectious agents among ticks, ticks comprising five species from two genera (hemaphysalis spp. and ixodes spp.) were screened using molecular techniques. ticks (3,135) were collected from small wild-caught mammals or by dragging/flagging in the republic of korea (rok) and were pooled into a total of 1,638 samples (1 to 27 ticks per pool). from the 1,638 tick samples, species-specific fragments of anaplasma phagocytophilum (1 sample), anaplasm ... | 2006 | 16957192 |
| ixodes philipi (acari: ixodidae): phylogenetic status inferred from mitochondrial cytochrome oxidase subunit i gene sequence comparison. | ixodes philipi ticks were collected from the nest burrows of streaked shearwaters, calonectris luecomelas, on 3 different islands of japan (awashima: 38 degrees 45'n, 139 degrees 24'e; mikurajima: 33 degrees 52'n, 139 degrees 36'e; and omorijima: 36 degrees 8'n, 133 degrees 10'e). the mitochondrial cytochrome oxidase subunit i (coi) gene sequence was determined for each tick. the coi sequences of 9 other ixodid tick species also were determined, and they were used for taxonomic positioning of i. ... | 2007 | 17626375 |
| multilocus sequence analysis of borrelia bissettii strains from north america reveals a new borrelia species, borrelia kurtenbachii. | using multilocus sequence analyses (mlsa), we investigated the phylogenetic relationship of spirochaete strains from north america previously assigned to the genospecies borrelia bissettii. we amplified internal fragments of 8 housekeeping genes (clpa, clpx, nifs, pepx, pyrg, recg, rplb, and uvra) located on the main linear chromosome by polymerase chain reaction. phylogenetic analysis of concatenated sequences of the 8 loci showed that the b. bissettii clade consisted of 4 closely related clust ... | 2010 | 21157575 |
| nested coevolutionary networks shape the ecological relationships of ticks, hosts, and the lyme disease bacteria of the borrelia burgdorferi (s.l.) complex. | the bacteria of the borrelia burgdorferi (s.l.) (bbg) complex constitute a group of tick-transmitted pathogens that are linked to many vertebrate and tick species. the ecological relationships between the pathogens, the ticks and the vertebrate carriers have not been analysed. the aim of this study was to quantitatively analyse these interactions by creating a network based on a large dataset of associations. specifically, we examined the relative positions of partners in the network, the phylog ... | 2016 | 27662832 |
| pathogenic potential and growth kinetics of muko virus in mice and human-derived cells. | ticks have been long known as vectors of various pathogens, some of which can cause high fatality rates among infected individuals. our enhanced tick surveillance around nagasaki, japan, led to the isolation and identification of a new strain of a recently identified orbivirus, muko virus (muv). the orbiviruses have a wide host range, including humans, and is related to a spectrum of clinical outcomes. however, the zoonotic potential of some members of the genus, although reported, were not clea ... | 2016 | 27777508 |
| prevalence of anaplasma and bartonella spp. in ticks collected from korean water deer (hydropotes inermis argyropus). | deer serve as reservoirs of tick-borne pathogens that impact on medical and veterinary health worldwide. in the republic of korea, the population of korean water deer (kwd, hydropotes inermis argyropus) has greatly increased from 1982 to 2011, in part, as a result of reforestation programs established following the korean war when much of the land was barren of trees. eighty seven haemaphysalis flava, 228 haemaphysalis longicornis, 8 ixodes nipponensis, and 40 ixodes persulcatus (21 larvae, 114 ... | 2016 | 26951985 |
| molecular detection of anaplasma, bartonella, and borrelia species in ticks collected from migratory birds from hong-do island, republic of korea. | bird migration is a recurring annual and seasonal event undertaken by more than 100 species of birds in the southeast asian and northeast palearctic regions that pass through or remain for short periods from april to may and september to november at hong-do island, republic of korea (rok). a total of 212 ticks (40 haemaphysalis flava, 12 h. longicornis, 146 ixodes turdus, 13 i. nipponensis, and 1 i. ornithophila) were collected from 65/2,161 (3.0%) migratory birds consisting of 21 species that w ... | 2013 | 23428091 |
| molecular detection of severe fever with thrombocytopenia syndrome and tick-borne encephalitis viruses in ixodid ticks collected from vegetation, republic of korea, 2014. | ticks play an important role in transmission of arboviruses responsible for emerging infectious diseases, and have a significant impact on human, veterinary, and wildlife health. in the republic of korea (rok), little is known about information regarding the presence of tick-borne viruses and their vectors. a total of 21,158 ticks belonging to 3 genera and 6 species collected at 6 provinces and 4 metropolitan areas in the rok from march to october 2014 were assayed for selected tick-borne pathog ... | 2016 | 27211914 |
| ticks collected from migratory birds, including a new record of haemaphysalis formosensis, on jeju island, korea. | migratory birds may disperse parasites across ecological barriers, and recent climate change may alter the pattern of ectoparasite dispersal via changed patterns of bird migration. in order to document the parasitization of migratory birds by ixodidae ticks on jeju island in korea, we examined 934 migratory birds comprising 75 species for ticks from 2010 to 2012. in total, 313 ticks were collected from 74 migratory birds across 17 avian species and identified based on morphological keys. these t ... | 2014 | 24141529 |
| genetic and biological characterization of muko virus, a new distinct member of the species great island virus (genus orbivirus, family reoviridae), isolated from ixodid ticks in japan. | among the tick-borne orbiviruses (genus orbivirus, family reoviridae), 36 serotypes are currently classified within a single virus species, great island virus. in this study, we report the first characterization of a tick-borne orbivirus isolated from the tick ixodes turdus in japan, which we identified as a new member of the species great island virus. the virus isolate, designated muko virus (muv), replicated and induced cytopathic effects in bhk-21, vero e6, and ccl-141 cells and caused high ... | 2015 | 26350980 |
| identification of tick species collected from wild boars and habitats of wild boars and domestic pigs in the republic of korea. | tick is one of the most important arthropods in the transmission of vector-borne diseases. in this study, we investigated the abundance and species of ticks associated with swine and their habitats to assess the risk of spread of tick-borne diseases in host species, such as wild boars. ticks were collected from 24 grazing or traditionally reared domestic pig farms and 8 habitats of wild boars in 8 provinces and 1 city in the republic of korea, by using the dragging and flagging methods. ticks we ... | 2017 | 28506041 |
| tick-borne pathogens in ticks collected from birds in taiwan. | a variety of human diseases transmitted by arthropod vectors, including ticks, are emerging around the globe. birds are known to be hosts of ticks and can disperse exotic ticks and tick-borne pathogens. in taiwan, previous studies have focused predominantly on mammals, leaving the role of birds in the maintenance of ticks and dissemination of tick-borne pathogens undetermined. | 2017 | 29178908 |
| a case of tick bite with ixodes turdus nakatsuji--a report from japan. | the first case of tick bite by ixodes turdus nakatsuji was reported. the tick was found on the occipital area near the left ear of a 6-year-old boy. the tick bite occurred in hoya, a suburb of tokyo. | 1990 | 2184177 |