| phylogenetic analysis of the genus desulfotomaculum: evidence for the misclassification of desulfotomaculum guttoideum and description of desulfotomaculum orientis as desulfosporosinus orientis gen. nov., comb. nov. | almost complete 16s ribosomal dna (rdna) sequences were determined for the type strains of nine species belonging to the genus desulfotomaculum and for seven strains described as strains of this genus. the sequences were compared with previously published 16s rdna and rrna sequences of the type strains of the other species of the genus. the majority of the species form a phylogenetically coherent cluster within the clostridium-bacillus subphylum of gram-positive bacteria. the cluster consists of ... | 1997 | 9336920 |
| spore-forming, desulfosporosinus-like sulphate-reducing bacteria from a shallow aquifer contaminated with gasoline. | previous studies on the geochemistry of a shallow unconfined aquifer contaminated with hydrocarbons suggested that the degradation of some hydrocarbons was linked to bacterial sulphate reduction. there was attenuation of naphthalene, 1,3,5-trimethylbenzene (tmb), toluene, p-xylene and ethylbenzene in the groundwater with concomitant loss of sulphate. here, the recovery of eight strains of sulphate-reducing bacteria (srb) from the contaminated site is reported. all were straight or curved rod-sha ... | 2000 | 10735993 |
| thiosulfate disproportionation by desulfotomaculum thermobenzoicum. | desulfotomaculum thermobenzoicum, but not desulfotomaculum nigrificans, desulfotomaculum ruminis, or desulfosporosinus orientis, grew by disproportionation of thiosulfate, forming stoichiometric amounts of sulfate and sulfide; sulfite was not disproportionated. the addition of acetate enhanced growth and thiosulfate disproportionation by d. thermobenzoicum compared to those observed with thiosulfate alone. | 2000 | 10919837 |
| desulfosporosinus meridiei sp. nov., a spore-forming sulfate-reducing bacterium isolated from gasolene-contaminated groundwater. | eight strains of spore-forming, sulfate-reducing bacteria, isolated from groundwater contaminated with motor fuel [mostly benzene, toluene ethylbenzene and xylene (btex) compounds] in sandy soil near perth, australia, were closely related to desulfosporosinus (previously desulfotomaculum) orientis dsm 765t (95.3-97.3% 16s rdna sequence similarity). whole-cell fatty acids were dominated by even-carbon, straight-chain saturated and mono-unsaturated fatty acids, in particular 16:0, 16:1cis9, 14:0 a ... | 2001 | 11211250 |
| separation of a phenol carboxylating organism from a two-member, strict anaerobic co-culture. | in a culture converting phenol to benzoic acid under anaerobic conditions and previously described as being constituted of only a clostridium-like strain 6, another bacterium (strain 7) was observed. each organism was enriched by centrifugation on a percoll gradient. strain 6 was purified by dilution and plating. strain 7 did not grow on solid media, but a strain 7 culture, cleared of strain 6, was obtained by subculturing in the presence of ampicillin and by dilution. in fresh medium, phenol wa ... | 2001 | 11400726 |
| the role of microbial populations in the containment of aromatic hydrocarbons in the subsurface. | a survey of soil gases associated with gasoline stations on the swan coastal plain of western australia has shown that 20% leak detectable amounts of petroleum. the fates of volatile hydrocarbons in the vadose zone at one contaminated site, and dissolved hydrocarbons in groundwater at another site were followed in a number of studies which are herein reviewed. geochemical evidence from a plume of hydrocarbon-contaminated groundwater has shown that sulfate reduction rapidly developed as the termi ... | 2002 | 12222956 |
| microbial populations stimulated for hexavalent uranium reduction in uranium mine sediment. | uranium-contaminated sediment and water collected from an inactive uranium mine were incubated anaerobically with organic substrates. stimulated microbial populations removed u almost entirely from solution within 1 month. x-ray absorption near-edge structure analysis showed that u(vi) was reduced to u(iv) during the incubation. observations by transmission electron microscopy, selected area diffraction pattern analysis, and energy-dispersive x-ray spectroscopic analysis showed two distinct type ... | 2003 | 12620814 |
| microorganisms associated with uranium bioremediation in a high-salinity subsurface sediment. | although stimulation of dissimilatory metal reduction to promote the reductive precipitation of uranium has been shown to successfully remove uranium from some aquifer sediments, the organisms in the family geobacteraceae that have been found to be associated with metal reduction in previous studies are not known to grow at the high salinities found in some uranium-contaminated groundwaters. studies with a highly saline uranium-contaminated aquifer sediment demonstrated that the addition of acet ... | 2003 | 12788780 |
| inhibiting mild steel corrosion from sulfate-reducing bacteria using antimicrobial-producing biofilms in three-mile-island process water. | biofilms were used to produce gramicidin s (a cyclic decapeptide) to inhibit corrosion-causing, sulfate-reducing bacteria (srb). in laboratory studies these biofilms protected mild steel 1010 continuously from corrosion in the aggressive, cooling service water of the amergen three-mile-island (tmi) nuclear plant, which was augmented with reference srb. the growth of both reference srb (gram-positive desulfosporosinus orientis and gram-negative desulfovibrio vulgaris) was shown to be inhibited by ... | 2004 | 12898064 |
| reclassification of desulfotomaculum auripigmentum as desulfosporosinus auripigmenti corrig., comb. nov. | the species desulfotomaculum auripigmentum is reclassified as desulfosporosinus auripigmenti corrig., comb. nov. on the basis of morphological and physiological traits, phylogenetic position and chemotaxonomic properties. characteristics supplementary to those provided in the original description reveal that the type strain, dsm 13351(t) (=atcc 700205(t)), forms oval, subterminal to terminal spores, possesses ll-diaminopimelic acid and contains mk-7 as the predominant menaquinone, while the whol ... | 2003 | 13130030 |
| sulfate-reducing bacteria-dominated biofilms that precipitate zns in a subsurface circumneutral-ph mine drainage system. | the microbial diversity of zns-forming biofilms in 8 degrees c, circumneutral-ph groundwater in tunnels within the abandoned piquette zn, pb mine (tennyson, wisconsin, usa) has been investigated by molecular methods, fluorescence in situ hybridization (fish), and cultivation techniques. these biofilms are growing on old mine timbers that generate locally anaerobic zones within the mine drainage system. sulfate-reducing bacteria (srb) exclusively of the family desulfobacteriaceae comprise a signi ... | 2004 | 14994175 |
| fish shows that desulfotomaculum spp. are the dominating sulfate-reducing bacteria in a pristine aquifer. | the hydrochemistry and the microbial diversity of a pristine aquifer system near garzweiler, germany, were characterized. hydrogeochemical and isotopic data indicate a recent activity of sulfate-reducing bacteria in the tertiary marine sands. the community structure in the aquifer was studied by fluorescence in situ hybridization (fish). up to 7.3 x 10(5) cells/ml were detected by dapi-staining. bacteria (identified by the probe eub338) were dominant, representing 51.9% of the total cell number ... | 2004 | 15085304 |
| inhibiting mild steel corrosion from sulfate-reducing and iron-oxidizing bacteria using gramicidin-s-producing biofilms. | a gramicidin-s-producing bacillus brevis 18-3 biofilm was shown to reduce corrosion rates of mild steel by inhibiting both the sulfate-reducing bacterium desulfosporosinus orientis and the iron-oxidizing bacterium leptothrix discophora sp-6. when l. discophora sp-6 was introduced along with d. orientis to a non-antimicrobial-producing biofilm control, paenibacillus polymyxa atcc 10401, a corrosive synergy was created and mild steel coupons underwent more severe corrosion than when only d. orient ... | 2004 | 15278311 |
| desulfosporomusa polytropa gen. nov., sp. nov., a novel sulfate-reducing bacterium from sediments of an oligotrophic lake. | five strains of sulfate-reducing bacteria were isolated from the highest positive dilutions of a most probable number (mpn) series supplemented with lactate and inoculated with sediments from the oligotrophic lake stechlin. the isolates were endospore-forming and were motile by means of laterally inserted flagella. they stained gram-negative and contained b-type cytochromes. co difference spectra indicated the presence of p582 as a sulfite reductase. phylogenetic analyses of the 16s rdna sequenc ... | 2004 | 15340785 |
| arsenate respiratory reductase gene (arra) for desulfosporosinus sp. strain y5. | desulfosporosinus sp. strain y5 is a spore-forming bacterium capable of dissimilatory arsenate reduction coupled to the oxidation of aromatic compounds. in arsenate respiration, the arsenate respiratory reductase (arr) catalyzes the reduction of arsenate to arsenite. our objective is to characterize the arra gene, encoding the arr, for desulfosporosinus sp. strain y5. oligonucleotide primers were designed based on the few arra gene sequences available at the time and validated against positive a ... | 2005 | 16242665 |
| sulfidogenesis in low ph (3.8-4.2) media by a mixed population of acidophilic bacteria. | a defined mixed bacterial culture was established which catalyzed dissimilatory sulfate reduction, using glycerol as electron donor, at ph 3.8-4.2. the bacterial consortium comprised a endospore-forming sulfate reducing bacterium (isolate m1) that had been isolated from acidic sediment in a geothermal area of montserrat (west indies) and which had 94% sequence identity (of its 16s rrna gene) to the gram-positive neutrophile desulfosporosinus orientis, and a gram-negative (non sulfate-reducing) a ... | 2006 | 16456614 |
| column experiments to assess the effects of electron donors on the efficiency of in situ precipitation of zn, cd, co and ni in contaminated groundwater applying the biological sulfate removal technology. | in a previous study, we explored the use of acetate, lactate, molasses, hydrogen release compound (hrc, which is based on a biodegradable poly-lactate ester), methanol and ethanol as carbon source and electron donor to promote bacterial sulfate reduction in batch experiments, this with regards to applying an in situ metal precipitation (ismp) process as a remediation tool to treat heavy metal contaminated groundwater at the site of a nonferrous metal work company. based on the results of these b ... | 2006 | 17120826 |
| desulfosporosinus lacus sp. nov., a sulfate-reducing bacterium isolated from pristine freshwater lake sediments. | a novel sulfate-reducing bacterium was isolated from pristine sediments of lake stechlin, germany. this strain, stp12(t), was found to contain predominantly c-type cytochromes and to reduce sulfate, sulfite and thiosulfate using lactate as an electron donor. although stp12(t) could not utilize elemental sulfur as an electron acceptor, it could support growth by dissimilatory fe(iii) reduction. in a comparison of 16s rrna gene sequences, stp12(t) was 96.7 % similar to desulfosporosinus auripigmen ... | 2006 | 17158969 |
| sulfidogenic fluidized-bed treatment of metal-containing wastewater at 8 and 65 degrees c temperatures is limited by acetate oxidation. | acetate utilization in sulfidogenic fluidized-bed reactors (fbrs) was investigated for the treatment of iron containing wastewater at low (8 degrees c) and high (65 degrees c) temperatures. the fbrs operated at low and high temperatures were inoculated with cultures of sulfate-reducing bacteria (srb) originally enriched from arctic and hot mining environments, respectively. acetate was not utilized as a carbon and electron source for srb at 8 degrees c. with ethanol, hydrogen sulfide was produce ... | 2007 | 17418880 |
| a highly sensitive hplc method for determination of nanomolar concentrations of dipicolinic acid, a characteristic constituent of bacterial endospores. | a high-performance liquid chromatographic method with indirect fluorescence detection has been developed for quantification of dipicolinic acid, a major constituent of bacterial endospores. after separation on a reversed-phase column, a post-column reagent of sodium acetate at 1 mol l(-1) with 50 micromol l(-1) terbium chloride was added for complexation of dipicolinic acid. terbium monodipicolinate complexes formed were quantified by measuring the fluorescence emission maximum at 548 nm after e ... | 2007 | 17573136 |
| prokaryotic community composition and ecology of floating macroscopic filaments from an extreme acidic environment, río tinto (sw, spain). | the prokaryotic diversity of macroscopic filaments located at the water surface in an extreme acidic environment, río tinto (sw, spain), has been analysed through denaturing gradient gel electrophoresis (dgge), cloning of 16s rrna genes and fluorescence in situ hybridization (fish). the dominant species present in the macrofilaments were acidithiobacillus ferrooxidans, leptospirillum ferrooxidans and acidiphilium spp., which represented the three main bacterial genera found in the water column o ... | 2007 | 17950555 |
| microbial sulfate reduction and metal attenuation in ph 4 acid mine water. | sediments recovered from the flooded mine workings of the penn mine, a cu-zn mine abandoned since the early 1960s, were cultured for anaerobic bacteria over a range of ph (4.0 to 7.5). the molecular biology of sediments and cultures was studied to determine whether sulfate-reducing bacteria (srb) were active in moderately acidic conditions present in the underground mine workings. here we document multiple, independent analyses and show evidence that sulfate reduction and associated metal attenu ... | 2007 | 17956615 |
| influence of nitrate on microbial reduction of pertechnetate. | factors influencing microbial reduction of tc(vii) in nitrate and radionuclide contaminated aquifer sediments were investigated using sediment microcosms containing organic electron donor, nitrate and tc(vii). microcosms underwent nitrate reduction followed by tc(vii) reduction. during this transition, the microbial community changed from being dominated by bacteria affiliated with the genus paenibacillus during the nitrate reduction phase, to those affiliated with genera agrobacterium, geothrix ... | 2008 | 18409612 |
| desulfosporosinus hippei sp. nov., a mesophilic sulfate-reducing bacterium isolated from permafrost. | the sulfate-reducing strain 343t was isolated from ancient permafrost deposits in siberia, russia. based on 16s rrna gene sequence analyses, this strain was closely related to desulfosporosinus species, showing 97.9 % 16s rrna gene sequence similarity to desulfosporosinus meridiei dsm 13257t, 97.6 % similarity to desulfosporosinus auripigmenti dsm 13351t, 97.2 % similarity to desulfosporosinus lacus dsm 15449t and 96.2 % similarity to desulfosporosinus orientis dsm 765t. the strain was found to ... | 2008 | 18450718 |
| microbial communities in contaminated sediments, associated with bioremediation of uranium to submicromolar levels. | microbial enumeration, 16s rrna gene clone libraries, and chemical analysis were used to evaluate the in situ biological reduction and immobilization of uranium(vi) in a long-term experiment (more than 2 years) conducted at a highly uranium-contaminated site (up to 60 mg/liter and 800 mg/kg solids) of the u.s. department of energy in oak ridge, tn. bioreduction was achieved by conditioning groundwater above ground and then stimulating growth of denitrifying, fe(iii)-reducing, and sulfate-reducin ... | 2008 | 18456853 |
| donor-dependent extent of uranium reduction for bioremediation of contaminated sediment microcosms. | bioremediation of uranium was investigated in microcosm experiments containing contaminated sediments from oak ridge, tennessee to explore the importance of electron donor selection for uranium reduction rate and extent. in these experiments, all of the electron donors, including ethanol, glucose, methanol, and methanol with added humic acids, stimulated the reduction and immobilization of aqueous uranium by the indigenous microbial community. uranium loss from solution began after the completio ... | 2009 | 19141795 |
| microbiological monitoring of acid mine drainage treatment systems and aquatic surroundings using real-time pcr. | in general, acid mine drainage (amd) causes low ph and high metal concentrations in mining areas and surroundings. the aim of this research was to achieve microbiological monitoring for amd and to assess whether mine water outflows have any ecological effects on the aqueous ecosystem receiving effluents from different types of treatment system. the water quality of aquatic sample was analyzed and the molecular biological diversity of the samples was assessed using 16s rrna methods, which were im ... | 2009 | 19494446 |
| microbial communities in subpermafrost saline fracture water at the lupin au mine, nunavut, canada. | we report the first investigation of a deep subpermafrost microbial ecosystem, a terrestrial analog for the martian subsurface. our multidisciplinary team analyzed fracture water collected at 890 and 1,130 m depths beneath a 540-m-thick permafrost layer at the lupin au mine (nunavut, canada). 14c, 3h, and noble gas isotope analyses suggest that the na-ca-cl, suboxic, fracture water represents a mixture of geologically ancient brine, approximately25-kyr-old, meteoric water and a minor modern tali ... | 2009 | 19568805 |
| an assessment of bacterial dysbiosis in pouchitis using terminal restriction fragment length polymorphisms of 16s ribosomal dna from pouch effluent microbiota. | previous studies on dysbiosis and pouchitis using conventional culture techniques have been disappointing because of inherent limitations associated with the technique. this study was designed to use terminal restriction fragment length polymorphism to evaluate patients with and without pouchitis. | 2009 | 19617766 |
| desulfosporosinus youngiae sp. nov., a spore-forming, sulfate-reducing bacterium isolated from a constructed wetland treating acid mine drainage. | strain jw/yjl-b18(t), a spore-forming, sulfate-reducing bacterium, was isolated from constructed wetland sediment. cells were curved rods, 0.7-1.2 mum in diameter and 3-7 mum long. despite being phylogenetically a member of the gram-type-positive phylum firmicutes, cells stained gram-negative at all growth phases. strain jw/yjl-b18(t) grew at 8-39 degrees c, with an optimum at 32-35 degrees c and no growth at 4 degrees c or below or at 42 degrees c or above. the ph(25 degrees c) range for growth ... | 2009 | 19625426 |
| a novel arsenate respiring isolate that can utilize aromatic substrates. | a novel anaerobic bacterium was isolated from the sediment of onondaga lake (syracuse, ny), which can use arsenate [as(v)] as a respiratory electron acceptor. the isolate, designated strain y5 is a spore-forming, motile rod, with lateral flagella. it is gram-negative though it phylogenetically falls within the low g + c gram-positive organisms. in addition to the more usual electron donors such as lactate and succinate, strain y5 also can use h(2)+ co(2) chemoautotrophically and metabolize aroma ... | 2004 | 19712302 |
| identification of rice root associated nitrate, sulfate and ferric iron reducing bacteria during root decomposition. | leakage of o(2) from roots of aquatic plants supports the oxidation of ammonia to nitrate and of sulfide to sulfate in the rhizosphere, so that these electron acceptors may become available to the root microbial communities and affect their activity. we studied the composition of the bacterial community active in anoxically incubated rice roots by analysis of terminal restriction fragment length polymorphism (t-rflp) and by cloning and sequencing targeting bacterial 16s rrna. the bacterial ribos ... | 2004 | 19712368 |
| sulfate reduction at ph 4.0 for treatment of process and wastewaters. | acidic industrial process and wastewaters often contain high sulfate and metal concentrations and their direct biological treatment is thus far not possible as biological processes at ph < 5 have been neglected. sulfate-reducing bacteria convert sulfate to sulfide that can subsequently be used to recover metals as metal-sulfides precipitate. this study reports on high-rate sulfate reduction with a mixed microbial community at ph 4.0 and 4.5 with hydrogen and/or formate as electron donors. the ma ... | 2010 | 20306533 |
| desulfosporosinus acidiphilus sp. nov.: a moderately acidophilic sulfate-reducing bacterium isolated from acid mining drainage sediments : new taxa: firmicutes (class clostridia, order clostridiales, family peptococcaceae). | an obligately anaerobic, spore-forming, acidophilic sulfate-reducing bacterium, strain sj4(t), was isolated from an acid mining effluent decantation pond sediment sample (ph around 3.0). cells were gram negative, non-motile, curved rods occurring singly. strain sj4(t) grew at ph 3.6-5.5 with an optimum at ph 5.2. strain sj4(t) utilized h(2), lactate, pyruvate, glycerol, glucose, and fructose as electron donors. lactate and glucose were weakly used. sulfate was used as electron acceptors, but not ... | 2010 | 20358236 |
| dna-sip identifies sulfate-reducing clostridia as important toluene degraders in tar-oil-contaminated aquifer sediment. | global groundwater resources are constantly challenged by a multitude of contaminants such as aromatic hydrocarbons. especially in anaerobic habitats, a large diversity of unrecognized microbial populations may be responsible for their degradation. still, our present understanding of the respective microbiota and their ecophysiology is almost exclusively based on a small number of cultured organisms, mostly within the proteobacteria. here, by dna-based stable isotope probing (sip), we directly i ... | 2010 | 20428224 |
| diversity of dissimilatory sulfite reductase genes (dsrab) in a salt marsh impacted by long-term acid mine drainage. | sulfate-reducing bacteria (srb) play a major role in the coupled biogeochemical cycling of sulfur and chalcophilic metal(loid)s. by implication, they can exert a strong influence on the speciation and mobility of multiple metal(loid) contaminants. in this study, we combined dsrab gene sequencing and sulfur isotopic profiling to identify the phylogeny and distribution of srb and to assess their metabolic activity in salt marsh sediments exposed to acid mine drainage (amd) for over 100 years. reco ... | 2010 | 20472728 |
| structure and function of the microbial community in an in situ reactor to treat an acidic mine pit lake. | sulfate-reducing bioreactors are a promising option for the treatment of acid mine drainage. we studied the structure and function of a biofilm in a methanol-fed fixed-bed in-lake reactor for the treatment of an acidic pit lake by a combination of laboratory incubations, chemical and molecular analyses and confocal laser scanning microscopy to determine whether competition by different groups of microorganisms as well as the precipitation of minerals affect reactor performance negatively. the bi ... | 2010 | 20491918 |
| a 'rare biosphere' microorganism contributes to sulfate reduction in a peatland. | methane emission from peatlands contributes substantially to global warming but is significantly reduced by sulfate reduction, which is fuelled by globally increasing aerial sulfur pollution. however, the biology behind sulfate reduction in terrestrial ecosystems is not well understood and the key players for this process as well as their abundance remained unidentified. comparative 16s rrna gene stable isotope probing (sip) in the presence and absence of sulfate indicated that a desulfosporosin ... | 2010 | 20535221 |
| microbial community changes in response to ethanol or methanol amendments for u(vi) reduction. | microbial community responses to ethanol, methanol, and methanol plus humics amendments in relationship to u(vi) bioreduction were studied in laboratory microcosm experiments using sediments and ground water from a uranium-contaminated site in oak ridge, tn. the type of carbon source added, the duration of incubation, and the sampling site influenced the bacterial community structure upon incubation. analysis of 16s rrna gene clone libraries indicated that (i) bacterial communities found in etha ... | 2010 | 20601514 |
| significant association between sulfate-reducing bacteria and uranium-reducing microbial communities as revealed by a combined massively parallel sequencing-indicator species approach. | massively parallel sequencing has provided a more affordable and high-throughput method to study microbial communities, although it has mostly been used in an exploratory fashion. we combined pyrosequencing with a strict indicator species statistical analysis to test if bacteria specifically responded to ethanol injection that successfully promoted dissimilatory uranium(vi) reduction in the subsurface of a uranium contamination plume at the oak ridge field research center in tennessee. remediati ... | 2010 | 20729318 |
| arsenic precipitation by an anaerobic arsenic-respiring bacterial strain isolated from the polluted sediments of orbetello lagoon, italy. | to isolate and characterize an anaerobic bacterial strain from the deeper polluted lagoon sediment able to use as electron acceptors [as(v)] and sulfate (so4(2-)), using lactate as an electron donor. | 2010 | 20860773 |
| the use of nucleic acid based stable isotope probing to identify the microorganisms responsible for anaerobic benzene and toluene biodegradation. | the remediation of sites contaminated with gasoline has been limited by a lack of information on the microorganisms able to transform these chemicals under anaerobic conditions. to address this, researchers have recently adopted a molecular method, called stable isotope probing (sip), to identify anaerobic toluene and benzene degraders from a number of environments across the globe. the approach involves incubation with (13)c labeled benzene or toluene, dna or rna extraction, ultracentrifugation ... | 2011 | 21356251 |
| geomicrobiology of la zarza-perrunal acid mine effluent (iberian pyritic belt, spain). | effluent from la zarza-perrunal, a mine on the iberian pyrite belt, was chosen to be geomicrobiologically characterized along a 1,200-m stream length. the ph at the origin was 3.1, which decreased to 1.9 at the final downstream sampling site. the total iron concentration showed variations along the effluent, resulting from (i) significant hydrolysis and precipitation of fe(iii) (especially along the first reach of the stream) and (ii) concentration induced by evaporation (mostly in the last reac ... | 2011 | 21357431 |
| heavy metal tolerance of fe(iii)-reducing microbial communities in contaminated creek bank soils. | fe(iii)-reducing soil enrichment cultures can tolerate 100 µm cu and cd, 150 µm co, 600 µm ni, and 2,500 µm zn. metal-tolerant cultures were dominated by geobacter-related deltaproteobacteria and gram-positive firmicutes spp. (clostridia and sedimentibacter). a cd- and cu-tolerant fe(iii)-reducing coculture of desulfosporosinus and desulfitobacterium indicated the importance of the firmicutes for fe(iii) reduction in the presence of metals. | 2011 | 21378042 |
| microbial diversity in anaerobic sediments at rio tinto, a naturally acidic environment with a high heavy metal content. | the tinto river is an extreme environment located at the core of the iberian pyritic belt (ipb). it is an unusual ecosystem due to its size (100 km long), constant acidic ph (mean ph, 2.3), and high concentration of heavy metals, iron, and sulfate in its waters, characteristics that make the tinto river basin comparable to acidic mine drainage (amd) systems. in this paper we present an extensive survey of the tinto river sediment microbiota using two culture-independent approaches: denaturing gr ... | 2011 | 21724883 |
| microbial community response to addition of polylactate compounds to stimulate hexavalent chromium reduction in groundwater. | to evaluate the efficacy of bioimmobilization of cr(vi) in groundwater at the department of energy hanford site, we conducted a series of microcosm experiments using a range of commercial electron donors with varying degrees of lactate polymerization (polylactate). these experiments were conducted using hanford formation sediments (coarse sand and gravel) immersed in hanford groundwater, which were amended with cr(vi) and several types of lactate-based electron donors (hydrogen release compound, ... | 2011 | 21872904 |
| Genome sequence of Desulfosporosinus sp. OT, an acidophilic sulfate-reducing bacterium from copper mining waste in Norilsk, Northern Siberia. | We have sequenced the genome of Desulfosporosinus sp. OT, a Gram-positive, acidophilic sulfate-reducing Firmicute isolated from copper tailing sediment in the Norilsk mining-smelting area in Northern Siberia, Russia. This represents the first sequenced genome of a Desulfosporosinus species. The genome has a size of 5.7 Mb and encodes 6,222 putative proteins. | 2011 | 21994931 |
| Iron transformations induced by an Acid-tolerant desulfosporosinus species. | The mineralogical transformations of Fe phases induced by an acid-tolerant, Fe(III)- and sulfate-reducing bacterium, Desulfosporosinus sp. strain GBSRB4.2 were evaluated under geochemical conditions associated with acid mine drainage-impacted systems (i.e., low pH and high Fe concentrations). X-ray powder diffractometry coupled with magnetic analysis by first-order reversal curve diagrams were used to evaluate mineral phases produced by GBSRB4.2 in media containing different ratios of Fe(II) and ... | 2012 | 22038606 |
| Diversity of Five Anaerobic Toluene Degrading Microbial Communities Investigated using Stable Isotope Probing (SIP). | Time-series DNA-stable isotope probing (SIP) was used to identify the microbes assimilating carbon from (13)C-toluene under nitrate or sulfate amended conditions in a range of inoculum sources, including uncontaminated and contaminated soil and wastewater treatment samples. In all, five different phylotypes were found to be responsible for toluene degradation and these included previously identified toluene degraders as well as novel toluene degrading microorganisms. In microcosms inoculated fro ... | 2011 | 22156434 |
| Enrichment of sulfate-reducing bacteria and resulting mineral formation in media mimicking pore water metal ion concentrations and pH conditions of acidic pit lakes. | Acid mine drainage sites are extreme environments with high acidity and metal ion concentrations. Under anoxic conditions, microbial sulfate reduction may trigger the formation of secondary minerals as a result of H(2)S production and pH increase. This process was studied in batch experiments with enrichment cultures from acidic sediments of a pit lake using growth media set at different pH values and containing elevated concentrations of Fe(2+) and Al(3+). At initial pH values of 5 and 6, sulfa ... | 2011 | 22066948 |