| comparative proteomic analysis of labellum and inner lateral petals in cymbidium ensifolium flowers. | the labellum in orchids shares homology with the inner lateral petals of the flower. the labellum is a modified petal and often distinguished from other petals and sepals due to its large size and irregular shape. herein, we combined two-dimensional gel electrophoresis (2-de) and matrix assisted laser desorption/ionization time of flight/time of flight (maldi-tof/tof) approaches to identify the differentially expressed proteome between labellum and inner lateral petal in one of orchid species (c ... | 2014 | 25365177 |
| molecular cloning and functional analysis of three flowering locus t (ft) homologous genes from chinese cymbidium. | the flowering locus t (ft) gene plays crucial roles in regulating the transition from the vegetative to reproductive phase. to understand the molecular mechanism of reproduction, three homologous ft genes were isolated and characterized from cymbidium sinense "qi jian bai mo", cymbidium goeringii and cymbidium ensifolium "jin si ma wei". the three genes contained 618-bp nucleotides with a 531-bp open reading frame (orf) of encoding 176 amino acids (aas). alignment of the aa sequences revealed th ... | 2012 | 23109860 |
| a putative mir172-targeted ceapetala2-like gene is involved in floral patterning regulation of the orchid cymbidium ensifolium. | apetala2 plays critical roles in establishing meristem and organ identity during plant floral development. in this study, we obtained a ceap2-like gene by using the mrna differential display technique to analyze the wild type and a multitepal mutant of the orchid cymbidium ensifolium. the full-length cdna encoding the ceap2-like transcription factor shows significant similarity to the cdna of ap2 from erycina pusilla and contains nucleotides complementary to mir172. using a transient gene expres ... | 2015 | 26505352 |
| characterization and comparative profiling of the small rna transcriptomes in two phases of flowering in cymbidium ensifolium. | cymbidium ensifolium is one of the most important ornamental flowers in china, with an elegant shape, beautiful appearance, and a fragrant aroma. its unique flower shape has long attracted scientists. micrornas (mirnas) are critical regulators in plant development and physiology, including floral development. however, to date, few studies have examined mirnas in c. ensifolium. | 2015 | 26289943 |
| digital gene expression analysis based on de novo transcriptome assembly reveals new genes associated with floral organ differentiation of the orchid plant cymbidium ensifolium. | cymbidium ensifolium belongs to the genus cymbidium of the orchid family. owing to its spectacular flower morphology, c. ensifolium has considerable ecological and cultural value. however, limited genetic data is available for this non-model plant, and the molecular mechanism underlying floral organ identity is still poorly understood. in this study, we characterize the floral transcriptome of c. ensifolium and present, for the first time, extensive sequence and transcript abundance data of indi ... | 2015 | 26580566 |
| duplicated c-class mads-box genes reveal distinct roles in gynostemium development in cymbidium ensifolium (orchidaceae). | the orchid floral organs represent novel and effective structures for attracting pollination vectors. in addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. identification of c-class mads-box genes regulating reproductive organ development could help determine the level of homology with the current abc model of floral organ identity in orchids. in this study, we isolated and characterized two c-class agamous-like genes, denoted cem ... | 2011 | 21278368 |
| first flowering hybrid between autotrophic and mycoheterotrophic plant species: breakthrough in molecular biology of mycoheterotrophy. | among land plants, which generally exhibit autotrophy through photosynthesis, about 880 species are mycoheterotrophs, dependent on mycorrhizal fungi for their carbon supply. shifts in nutritional mode from autotrophy to mycoheterotrophy are usually accompanied by evolution of various combinations of characters related to structure and physiology, e.g., loss of foliage leaves and roots, reduction in seed size, degradation of plastid genome, and changes in mycorrhizal association and pollination s ... | 2014 | 24310615 |
| deep sequencing-based analysis of the cymbidium ensifolium floral transcriptome. | cymbidium ensifolium is a chinese cymbidium with an elegant shape, beautiful appearance, and a fragrant aroma. c. ensifolium has a long history of cultivation in china and it has excellent commercial value as a potted plant and cut flower. the development of c. ensifolium genomic resources has been delayed because of its large genome size. taking advantage of technical and cost improvement of rna-seq, we extracted total mrna from flower buds and mature flowers and obtained a total of 9.52 gb of ... | 2013 | 24392013 |
| concurrent changes in methyl jasmonate emission and the expression of its biosynthesis-related genes in cymbidium ensifolium flowers. | methyl jasmonate (meja) is one of most abundant scent compounds in cymbidium ensifolium flowers. in this study, the emission of meja and its regulation mechanism were investigated. our results showed that emission of meja in c. ensifolium flowers was controlled developmentally and rhythmically. it occurred in a tissue-specific manner, and high meja emission was found in sepals and petals. a group of vital genes involved in the meja biosynthesis via the octadecanoid pathway were isolated from c. ... | 2015 | 25214235 |
| development of cymbidium ensifolium genic-ssr markers and their utility in genetic diversity and population structure analysis in cymbidiums. | cymbidium is a genus of 68 species in the orchid family, with extremely high ornamental value. marker-assisted selection has proven to be an effective strategy in accelerating plant breeding for many plant species. analysis of cymbidiums genetic background by molecular markers can be of great value in assisting parental selection and breeding strategy design, however, in plants such as cymbidiums limited genomic resources exist. in order to obtain efficient markers, we deep sequenced the c. ensi ... | 2014 | 25481640 |