| phylogeny of the glomerales and diversisporales (fungi: glomeromycota) from actin and elongation factor 1-alpha sequences. | the arbuscular mycorrhizal (am) fungi have been elevated to the phylum glomeromycota based on a ribosomal gene phylogeny. in order to test this phylogeny, we amplified and sequenced small subunit ribosomal rna (ssurrna), actin and elongation factor 1 (ef1)-alpha gene fragments from single spores of acaulospora laevis, glomus caledonium, gigaspora margarita, and scutellospora dipurpurescens. sequence variation within and among spores of an isolate was low except for ssurrna in s. dipurpurescens, ... | 2003 | 14659552 |
| assessing the diversity of am fungi in arid gypsophilous plant communities. | in the present study, we used pcr-single-stranded conformation polymorphism (sscp) techniques to analyse arbuscular mycorrhizal fungi (amf) communities in four sites within a 10 km(2) gypsum area in southern spain. four common plant species from these ecosystems were selected. the am fungal small-subunit (ssu) rrna genes were subjected to pcr, cloning, sscp analysis, sequencing and phylogenetic analyses. a total of 1443 ssu rrna sequences were analysed, for 21 am fungal types: 19 belonged to the ... | 2009 | 19573133 |
| arbuscular mycorrhiza of arnica montana under field conditions--conventional and molecular studies. | two distinct populations of arnica montana, an endangered medicinal plant, were studied under field conditions. the material was investigated using microscopic and molecular methods. the analyzed plants were always found to be mycorrhizal. nineteen arbuscular mycorrhizal fungal dna sequences were obtained from the roots. they were related to glomus group a, but most did not match any known species. some showed a degree of similarity to fungi colonizing liverworts. conventional analysis of spores ... | 2010 | 20195874 |