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pseudomonas gessardii sp. nov. and pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.twenty-five non-identified fluorescent pseudomonas strains isolated from natural mineral waters were previously clustered into three phenotypic subclusters, xiiib, xva and xvc. these strains were characterized genotypically in the present study. dna-dna hybridization results and dna base composition analysis revealed that these strains were members of two new species, for which the names pseudomonas gessardii sp. nov. (type strain cip 105469t) and pseudomonas migulae sp. nov. (type strain cip 10 ...199910555337
production of a novel extracellular acidic lipase from pseudomonas gessardii using slaughterhouse waste as a substrate.an isolate exhibiting high extracellular lipolytic activity was identified as pseudomonas gessardii by 16s rdna gene sequence analysis. the slaughterhouse waste, goat tallow, was used as a lipid substrate for the production of acidic lipase by p. gessardii. the maximum lipase activity of 156 u/ml was observed at an acidic ph of 3.5 and at 0.31 g substrate concentration. the purification steps resulted in the isolation of acidic lipase with a specific activity of 1,473 u/mg and a molecular weight ...201020204455
isolation, identification and characterization of a new lipolytic pseudomonas sp., strain ahd-1, from tunisian soil.a novel, lipid-degrading bacterium (strain ahd-1) was isolated from soil regularly contaminated with washing-machine wastewater in sfax, tunisia. when this strain was grown in a medium containing 2% triacylglycerol, the hydrolysis products were found to be diacylglycerols, monoacylglycerols and free fatty acids. this strain was an aerobic, mesophilic, gram-negative, motile, non-sporulating bacterium, capable of growing optimally at ph 7 and 27 degrees c. the predominant fatty acids were found to ...201020232682
influence of storage conditions on the growth of pseudomonas species in refrigerated raw milk.the refrigerated storage of raw milk throughout the dairy chain prior to heat treatment creates selective conditions for growth of psychrotolerant bacteria. these bacteria, mainly belonging to the genus pseudomonas, are capable of producing thermoresistant extracellular proteases and lipases, which can cause spoilage and structural defects in pasteurized and ultra-high-temperature-treated milk (products). to map the influence of refrigerated storage on the growth of these pseudomonads, milk samp ...201021115713
low bacterial diversity and high labile organic matter concentrations in the sediments of the medee deep-sea hypersaline anoxic basin.studies in the center and margin of the medee basin, a mediterranean deep-sea hypersaline anoxic basin, and at a reference site during penelope cruise (2007), revealed the existence of a 7 m-thick halocline, with high salinity (328 psu), and high sedimentary organic carbon and biopolymer concentrations. the 194 16s rrna sequences retrieved were grouped into 118 unique phylotypes. pseudomonas gessardii, dominated in the center, while 33 phylotypes were detected at the margin and 73 at the referen ...201222504432
pseudomonads rule degradation of polyaromatic hydrocarbons in aerated sediment.given that the degradation of aromatic pollutants in anaerobic environments such as sediment is generally very slow, aeration could be an efficient bioremediation option. using stable isotope probing (sip) coupled with pyrosequencing analysis of 16s rrna genes, we identified naphthalene-utilizing populations in aerated polyaromatic hydrocarbon (pah)-polluted sediment. the results showed that naphthalene was metabolized at both 10 and 20°c following oxygen delivery, with increased degradation at ...201526635740
possible misidentification of species in the pseudomonas fluorescens lineage as burkholderia pseudomallei and francisella tularensis, and emended descriptions of pseudomonas brenneri,pseudomonas gessardii and pseudomonas proteolytica.bacteria were isolated from an industrial water circuit in the netherlands. these strains were identified using api 20 ne as possible, or likely, burkholderia pseudomallei. with vitek 2 some of these strains scored 'low discrimination' for francisella tularensis, amongst others. a total of twenty-six strains were assigned to the species pseudomonas brenneri, pseudomonas gessardii or pseudomonas proteolytica. because of the possibility of misidentification of these environmental species as medica ...201627259517
a novel pseudomonas gessardii strain lz-e simultaneously degrades naphthalene and reduces hexavalent chromium.combined pollutants with polycyclic aromatic hydrocarbons (pahs) and heavy metals have been identified as toxic and unmanageable contaminates. in this work, pseudomonas gessardii strain lz-e isolated from wastewater discharge site of a petrochemical company degrades naphthalene and reduces cr(vi) simultaneously. 95% of 10mgl(-1) cr(vi) was reduced to cr(iii) while 77% of 800mgl(-1) naphthalene was degraded when strain lz-e was incubated in bh medium for 48h. furthermore, naphthalene promotes cr( ...201626901089
impact of lead tolerant plant growth promoting rhizobacteria on growth, physiology, antioxidant activities, yield and lead content in sunflower in lead contaminated soil.present study was conducted to evaluate the effect of lead tolerant plant growth promoting rhizobacteria (ltpgpr) on growth, physiology, yield, antioxidant activities and lead uptake in sunflower in soil contaminated with lead under pot conditions. three pre-characterized ltpgp strains (s2 (pseudomonas gessardii strain blp141), s5 (pseudomonas fluorescens a506) and s10 (pseudomonas fluorescens strain lmg 2189)) were used to inoculate sunflower growing in soil contaminated with different levels ( ...201829278850
population structure and diversity of phenazine-1-carboxylic acid producing fluorescent pseudomonas spp. from dryland cereal fields of central washington state (usa).certain strains of the rhizosphere bacterium pseudomonas fluorescens contain the phenazine biosynthesis operon (phzabcdefg) and produce redox-active phenazine antibiotics that suppress a wide variety of soilborne plant pathogens. in 2007 and 2008, we isolated 412 phenazine-producing (phz(+)) fluorescent pseudomonas strains from roots of dryland wheat and barley grown in the low-precipitation region (<350 mm annual precipitation) of central washington state. based on results of box-pcr genomic fi ...201222383119
fluorescent pseudomonads in the phyllosphere of wheat: potential antagonists against fungal phytopathogens.fluorescent pseudomonads isolated from wheat leaves were characterized regarding their antagonistic potential and taxonomy in relation to protect crop plants from infestation by fusarium and alternaria fungi causing diseases in wheat. using a dual culture assay, inhibition of fungal growth was found for 40 isolates of 175 fluorescent pseudomonads. twenty-two of the antagonists were able to suppress strains of fusarium as well as alternaria. by means of real-time qpcr, the phld gene encoding the ...201626687461
pseudomonas yamanorum sp. nov., a psychrotolerant bacterium isolated from a subantarctic environment.a psychrotolerant strain, 8h1(t), was isolated from soil samples collected in isla de los estados, ushuaia, argentina. cells were gram-negative, aerobic, straight rods, occurring singly or in pairs, non-spore-forming and motile by means of two polar flagella. the isolate was able to grow in the range 4-35 °c, with optimum growth at 28 °c. the predominant cellular fatty acids were summed feature 3 (c16 : 1ω6c and/or c16 : 1ω7c), c16 : 0 and summed feature 8 (c18 : 1ω6c and/or c18 : 1ω7c). the pol ...201525385990
pseudomonas lactis sp. nov. and pseudomonas paralactis sp. nov., isolated from bovine raw milk.five strains, designated ws 4672t, ws 4998, ws 4992t, ws 4997, and ws 5000, isolated from bovine raw milk formed two individual groups in a phylogenetic analysis. the most similar species on the basis of 16s rrna gene sequences were pseudomonas azotoformans iam 1603t, pseudomonas gessardii cip 105469t, and pseudomonas libanensis cip 105460t showing 99.7-99.6 % similarity. using rpod gene sequences pseudomonas veronii lmg 17761t (93.3 %) was most closely related to strain ws 4672t and pseudomonas ...201728141500
production of lipase from pseudomonas gessardii using blood tissue lipid and thereof for the hydrolysis of blood cholesterol and triglycerides and lysis of red blood cells.the study demonstrates the production of lipase (lip) from pseudomonas gessardii using blood tissue lipid as the substrate for the hydrolysis of blood cholesterol and triglycerides. the lipase was purified with the specific activity of 828 u/mg protein and the molecular weight of 56 kda. the maximum lipase activity was observed at the ph 7.0 and the temperature 37 °c. the amino acid composition of purified lipase was determined by hplc. the mesoporous activated carbon (mac) was used for the immo ...201222228367
pseudomonas grimontii sp. nov.the vernacular name 'fluorescent pseudomonas group 97-514' was coined for a group of 43 strains isolated from two french natural mineral waters. all these strains were gram-negative, rod-shaped and motile by means of a single polar flagellum. they produced fluorescent pigment (pyoverdin) on king b medium, catalase and cytochrome oxidase. they were capable of respiratory but not fermentative metabolism. they were not able to accumulate poly-beta-hydroxybutyrate and possessed an arginine dihydrola ...200212361251
microbial induced lipoprotein biosurfactant from slaughterhouse lipid waste and its application to the removal of metal ions from aqueous solution.this study aims at demonstrating the production of lipoprotein biosurfactant from pseudomonas gessardii using goat tallow, a slaughterhouse lipid waste, as the substrate and its application to the removal of metal ions from aqueous solution. the maximum bio-transformation of goat tallow into biosurfactant occurred at 48 h. the mass of the lipoprotein biosurfactant produced was 2.03 g/g of goat tallow. the biosurfactant was clearly characterized by surface tension, critical micelle concentration, ...201222592059
characterization of microbial population of breba and main crops (ficus carica) during cold storage: influence of passive modified atmospheres (map) and antimicrobial extract application.the purpose of this work was to study the changes of bacterial and fungal population of breba fruits such as 'banane' and 'san antonio' as well as 'cuello dama negro', 'cuello dama blanco' and 'san antonio' fig cultivars stored in passive modified atmospheres (map) by the use of three different microperforated films (m10 with 16 holes; m30 with five holes and m50 with three holes). moreover the effects of the application of aqueous soy polyphenolic antimicrobial extract (ape), alone or combined ...201728040180
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