pseudomonas gessardii sp. nov. and pseudomonas migulae sp. nov., two new species isolated from natural mineral waters.twenty-five non-identified fluorescent pseudomonas strains isolated from natural mineral waters were previously clustered into three phenotypic subclusters, xiiib, xva and xvc. these strains were characterized genotypically in the present study. dna-dna hybridization results and dna base composition analysis revealed that these strains were members of two new species, for which the names pseudomonas gessardii sp. nov. (type strain cip 105469t) and pseudomonas migulae sp. nov. (type strain cip 10 ...199910555337
detection of bacterial dna in latin american patients with reactive arthritis by polymerase chain reaction and sequencing analysis.bacteria and/or their antigens are thought to play a role in the pathogenesis of reactive arthritis (rea). polymerase chain reaction (pcr) using the 16s ribosomal rna-pcr method was used to identify bacterial dna in synovial fluid (sf) and tissue (st) in a well defined group of patients with chronic rea. in addition, species found were identified by means of sequence analysis.200212136901
pseudomonas koreensis sp. nov., pseudomonas umsongensis sp. nov. and pseudomonas jinjuensis sp. nov., novel species from farm soils in korea.among pseudomonas strains isolated from korean agricultural soils, four strains (ps 9-14 group: ps 1-2, ps 1-10, ps 5-5 and ps 9-14t) from the suwon, goesan and samchok regions, three strains (ps 3-10 group: ps 2-22, ps 3-1 and ps 3-10t) from umsong region and four strains (pss 26 group: pss 14, pss 25, pss 26t and pss 27) from jinju region were identified as three independent groups on the basis of 16s rdna sequence analysis. while, on the basis of 16s rdna sequence analysis, ps 9-14t and ps 3- ...200312656147
synergistic relationships in algal-bacterial microcosms for the treatment of aromatic pollutants.the potential of algal-bacterial microcosms was studied for the biodegradation of salicylate, phenol and phenanthrene. the isolation and characterization of aerobic bacterial strains capable of mineralizing each pollutant were first conducted. ralstonia basilensis was isolated for salicylate degradation, acinetobacter haemolyticus for phenol and pseudomonas migulae and sphingomonas yanoikuyae for phenanthrene. the green alga chlorella sorokiniana was then cultivated in the presence of the pollut ...200312688473
phenanthrene biodegradation by an algal-bacterial consortium in two-phase partitioning algal-bacterial consortium formed by chlorella sorokiniana and a phenanthrene-degrading pseudomonas migulae strain was able to biodegrade 200-500 mg/l of phenanthrene dissolved in silicone oil or tetradecane under photosynthetic conditions and without any external supply of oxygen. phenanthrene was only removed when provided in organic solvent, which confirms the potential of two-phase systems for toxicity reduction. phenanthrene was degraded at highest rates when provided in silicone oil rat ...200312698286
psychrophilic pseudomonads from antarctica: pseudomonas antarctica sp. nov., pseudomonas meridiana sp. nov. and pseudomonas proteolytica sp. nov.thirty-one bacteria that belonged to the genus pseudomonas were isolated from cyanobacterial mat samples that were collected from various water bodies in antarctica. all 31 isolates were psychrophilic; they could be divided into three groups, based on their protein profiles. representative strains of each of the three groups, namely cms 35(t), cms 38(t) and cms 64(t), were studied in detail. based on 16s rrna gene sequence analysis, it was established that the strains were related closely to the ...200415143013
pseudomonas panacis sp. nov., isolated from the surface of rusty roots of korean ginseng.a gram-negative, aerobic bacterium, designated cg20106(t), was isolated from the surface tissues of rusty root lesions of korean ginseng. phylogenetic analysis of the 16s rrna gene sequence revealed that this isolate represents a hitherto unknown subline within the genus pseudomonas. strain cg20106(t) was catalase- and oxidase-positive, motile and rod-shaped. the overall phenotypic features of the ginseng isolate were similar to those of pseudomonas cedrina, pseudomonas migulae and pseudomonas a ...200516014508
genetic diversity of culturable bacteria in oil-contaminated rhizosphere of galega orientalis.a collection of 50 indigenous meta-toluate tolerating bacteria isolated from oil-contaminated rhizosphere of galega orientalis on selective medium was characterized and identified by classical and molecular methods. 16s rdna partial sequencing showed the presence of five major lineages of the bacteria domain. gram-positive rhodococcus, bacillus and arthrobacter and gram-negative pseudomonas were the most abundant genera. only one-fifth of the strains that tolerated m-toluate also degraded m-tolu ...200616055251
aerobic phenanthrene biodegradation in a two-phase partitioning bioreactor.the aerobic degradation of phenanthrene by a pseudomonas migulae strain under classical mechanical aeration and under photosynthetic oxygenation (using a chlorella sorokiniana strain) in a two-phase partitioning bioreactor (tppb) constructed with silicone oil as organic phase was investigated. when traditional mechanical aeration was used, an increase in the aeration and/or in the agitation rate enhanced phenanthrene biodegradation. thus, phenanthrene removal rates (based on the total liquid vol ...200516312976
pseudomonas peli sp. nov. and pseudomonas borbori sp. nov., isolated from a nitrifying inoculum.sixteen gram-negative, rod-shaped, non-spore-forming isolates were obtained from a nitrifying inoculum. analysis of repetitive sequence-based pcr and sds-page banding patterns, 16s rrna gene sequence analysis and dna-dna hybridizations showed that the isolates belonged to various groups within the genus pseudomonas. one group of isolates could be assigned to pseudomonas migulae and a second to pseudomonas veronii. two groups could be differentiated genotypically from each other and from all othe ...200616902024
pseudomonas pohangensis sp. nov., isolated from seashore sand in korea.a novel bacterium, designated strain h3-r18(t), was isolated from seashore sand collected from homi cape, pohang city, korea. cells were gram-negative, aerobic, non-motile, cream-coloured, mesophilic and slightly halotolerant. 16s rrna gene sequence analysis indicated that the organism was a member of the genus pseudomonas, but the sequence showed < or =96.3 % sequence similarity to that of the type strains of all recognized pseudomonas species. highest sequence similarities were to pseudomonas ...200616957113
tol plasmid transfer during bacterial conjugation in vitro and rhizoremediation of oil compounds in vivo.molecular profiling methods for horizontal transfer of aromatics-degrading plasmids were developed and applied during rhizoremediation in vivo and conjugations in vitro. pww0 was conjugated from pseudomonas to rhizobium. the xyle gene was detected both in rhizobium galegae bv. officinalis and bv. orientalis, but it was neither stably maintained in orientalis nor functional in officinalis. tol plasmids were a major group of catabolic plasmids among the bacterial strains isolated from the oil-cont ...200717000041
draft genome sequence of se(iv)-reducing bacterium pseudomonas migulae es3-33.pseudomonas migulae es3-33 is a gram-negative strain that strongly reduces se(iv) and was isolated from a selenium mining area in enshi, southwest china. here we present the draft genome of this strain containing potential genes involved in selenite reduction and a large number of genes encoding resistances to copper and antibiotics.201525953191
isolation & identification of bacteria for the treatment of brown crab (cancer pagurus) waste to produce chitinous isolate bacteria from soil for microbial pretreatment of brown crab (cancer pagurus) shell waste and the production of chitin.201525644656
complete nucleotide sequence of the self-transmissible tol plasmid pd2rt provides new insight into arrangement of toluene catabolic the present study we report the complete nucleotide sequence of the toluene catabolic plasmid pd2rt of pseudomonas migulae strain d2rt isolated from baltic sea water. the pd2rt is 129,894 base pairs in size with an average g+c content of 53.75%. a total of 135 open reading frames (orfs) were predicted to encode proteins, among them genes for catabolism of toluene, plasmid replication, maintenance and conjugative transfer. orfs encoding proteins with putative functions in stress response, tran ...201324095800
amelioration of high salinity stress damage by plant growth-promoting bacterial endophytes that contain acc deaminase.plant growth and productivity is negatively affected by soil salinity. however, it is predicted that plant growth-promoting bacterial (pgpb) endophytes that contain 1-aminocyclopropane-1-carboxylate (acc) deaminase (e.c. can facilitate plant growth and development in the presence of a number of different stresses. in present study, the ability of acc deaminase containing pgpb endophytes pseudomonas fluorescens yss6, pseudomonas migulae 8r6, and their acc deaminase deficient mutants to ...201424769617
delay of flower senescence by bacterial endophytes expressing 1-aminocyclopropane-1-carboxylate deaminase.the ability of 1-aminocyclopropane-1-carboxylate (acc) deaminase-containing plant growth-promoting bacterial (pgpb) endophytes pseudomonas fluorescens yss6 and pseudomonas migulae 8r6, their acc deaminase minus mutants and the rhizospheric plant growth-promoting bacterium pseudomonas putida uw4 to delay the senescence of mini carnation cut flowers was assessed.201222816486
efficacy of forming biofilms by pseudomonas migulae an-1 toward in situ bioremediation of aniline-contaminated aquifer by groundwater circulation wells.the formation and activity of aniline-degrading biofilms developed by the psychrotrophic pseudomonas migulae an-1 were studied for the in situ remediation of contaminated aquifer using in-well bioreactor of groundwater circulating wells (gcws). biofilms grown in mineral salt medium with aniline exhibited tolerance to high concentrations of aniline. in aniline degradation rate, an-1 biofilms exhibited slight differences compared with planktonic cells. the effectiveness and bio-implication of an-1 ...201627115704
bioaugmentation with gfp-tagged pseudomonas migulae an-1 in aniline-contaminated aquifer microcosms: cellular responses, survival and effect on indigenous bacterial community.the recently isolated aniline-degrading bacterium pseudomonas migulae an-1 was tagged with green fluorescent protein (gfp) to investigate its bioaugmentation potential against anilinecontaminated groundwater through microcosm experiments. the survival and cellular response of gfp-tagged an-1 introduced in a lab-scale aquifer corresponded directly with aniline consumption. during the process, the gfp-tagged an-1 biomass increased from 7.52 × 10⁵ cells/ml to 128 × 10⁵ cells/ml and the degradation ...201626907759
low-temperature biodegradation of aniline by freely suspended and magnetic modified pseudomonas migulae an-1.aniline is of great environmental concern with regards to widespread occurrence in water and soil and increasing threat into the life forms. bioremediation involving the use of degrading bacterium in the removal of aniline is the most promising process, yet inhibited under low temperature usually. in the present study, a new psychrotrophic bacterial strain isolated from groundwater, designated an-1, was shown to be capable of aniline degradation in a concentration range of 135-2202 mg l(-1) with ...201525620371
growth promotory potential of the cold adapted diazotroph pseudomonas migulae s10724 against native green gram (vigna radiata (l.) wilczek).it is being confirmed previously the atmospheric nitrogen fixing ability of the cold adapted pseudomonas migulae s10724 strain at the fluctuating temperatures. therefore, net house bioinoculation experiment was performed to determine the effectiveness of inoculation of strain s10724 on the growth enhancement of native green gram (vigna radiata l. wilczek). the strain significantly (p < 0.05) stimulated the growth of roots (45.3 %) and shoots (45.6 %) of green gram plants. furthermore, other grow ...201428324315
differential proteomics in response to low temperature diazotrophy of himalayan psychrophilic nitrogen fixing pseudomonas migulae s10724 strain.himalayas are considered as a reservoir of diversified and dynamic gene pool. this study describes the response of a himalayan psychrophilic diazotroph to low temperature diazotrophy. seven cold adaptive n2 fixing bacteria were isolated and identified as bacillus sp., arthrobacter sp., rhodococcus sp., pseudomonas sp., etc. in order to examine the physiological response to low temperature diazotrophy, differential proteomic analysis of pseudomonas migulae s10724 strain was carried out using two ...201424362552
visualisation study on pseudomonas migulae an-1 transport in saturated porous media.influence of granular size and groundwater flow rate on transport of pseudomonas migulae an-1 in saturated porous media was non-invasively and visually investigated with a novel imaging technique based on our previously established green fluorescent protein-tagging approach. an-1 was transported faster than water was. the finer the media were, the greater the enhancement of bacterial velocity was. mass recovery (mr) increased, while deposition rate coefficient (kc) decreased, with increasing gra ...201728618357
insights into the fecal microbiota of captive iberian lynx (lynx pardinus).the iberian lynx (lynx pardinus) is an endangered species restricted to several areas of spain and portugal. its low genetic diversity likely provokes immune depression and high susceptibility to infectious diseases. the intestinal microbiota is closely related to host health and nutrition. in order to contribute to the knowledge of the iberian lynx intestinal microbiota, fecal microbiota of captive specimens from two breeding centers ("la olivilla" and "el acebuche"), located in southern spain, ...201728581020
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