| pcr and restriction fragment length polymorphism of a pel gene as a tool to identify erwinia carotovora in relation to potato diseases. | using a sequenced pectate lyase-encoding gene (pel gene), we developed a pcr test for erwinia carotovora. a set of primers allowed the amplification of a 434-bp fragment in e. carotovora strains. among the 89 e. carotovora strains tested, only the erwinia carotovora subsp. betavasculorum strains were not detected. a restriction fragment length polymorphism (rflp) study was undertaken on the amplified fragment with seven endonucleases. the sau3ai digestion pattern specifically identified the erwi ... | 1994 | 7912502 |
| genotyping of bacteria belonging to the former erwinia genus by pcr-rflp analysis of a reca gene fragment. | genotypic characterization, based on the analysis of restriction fragment length polymorphism of the reca gene fragment pcr product (reca pcr-rflp), was performed on members of the former erwinia genus. pcr primers deduced from published reca gene sequences of erwinia carotovora allowed the amplification of an approximately 730 bp dna fragment from each of the 19 erwinia species tested. amplified reca fragments were compared using rflp analysis with four endonucleases (alui, hinfi, tasi and tru1 ... | 2002 | 11832521 |
| application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria erwinia carotovora and erwinia chrysanthemi. | the soft rot bacteria erwinia carotovora and erwinia chrysanthemi are important pathogens of potato and other crops. however, the taxonomy of these pathogens, particularly at subspecies level, is unclear. an investigation using amplified fragment length polymorphism (aflp) fingerprinting was undertaken to determine the taxonomic relationships within this group based on their genetic relatedness. following cluster analysis on the similarity matrices derived from the aflp gels, four clusters (clus ... | 2002 | 11916661 |
| taxonomic relatedness between pectobacterium carotovorum subsp. carotovorum, pectobacterium carotovorum subsp. odoriferum and pectobacterium carotovorum subsp. brasiliense subsp. nov. | pectobacterium carotovorum is a heterogeneous species consisting of two named subspecies, p. carotovorum subsp. carotovorum and p. carotovorum subsp. odoriferum. a third subspecies, p. carotovorum subsp. brasiliense, was previously proposed. the study aimed to confirm the subspecies status and validate the proposed name of p. carotovorum subsp. brasiliense using a novel and standard microbial taxonomy. | 2012 | 22747943 |