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[physiological studies on konnyaku. part 2. hypertensive action of konnyaku]. 19761036211
[inhibitory effect of refined amorphophallus konjac on mnng-induced lung cancers in mice].550 seven-wk-old laca mice were used in 3 batches for studying the inhibitory effect of refined amorphophallus konjac (konjaku powder) on mnng-induced lung cancers. the mice (within each batch) were randomly allocated into four groups, namely, positive control (mnng), amorphophallus konjac (a. k.), complex (mnng+a. k.), and blank control (c) groups. in mnng group, mnng (250 micrograms) was injected intravenously once every five days for seven times in each mouse, the total dosage of mnng being 1 ...19921327690
[antisteatotic effects of four kinds of dietary fibers in rats fed on high cholesterol diet: a preliminary morphometric analysis].the hypolipidaemic and antisteatotic effects of the konnyaku powder (kp) have been reported before. in order to evaluate further the antisteatotic role played by kp, the effects of kp on the levels of liver lipid and on the hepatic histopathology and morphometry in comparison with those of pectin, algin and agar were studied. sixty wistar strain rats were divided into 6 groups: a normal diet group, a high cholesterol diet group (hc), and 4 test groups, in which the animals were fed on a diet sim ...19921328028
antigen-induced histamine release from peripheral leukocytes of "konnyaku asthmatic patients".histamine release from peripheral leukocytes of "konnyaku asthmatic patients," patients with a unique occupational bronchial asthma in japan, was measured after challenge with the antigen. there was an antigen concentration-dependent response with highly significant correlations between the maximal histamine release and the ratio of the radioallergosorbent test (rast) of the antigen to human serum albumin. these results suggest that the histamine release test from peripheral leukocytes as well a ...19901689973
[prophylactic effect of refined amorphophallus konjac on mnng-induced lung cancers in mice].three hundred and seventy laca mice of seven weeks old were used in 2 batches for studying the prophylactic effect of konjaku powder on mnng-induced lung cancers. these mice (within each batch) were randomly allocated to four groups, namely positive control (mnng), refined amorphophallus konjac (r.a.k.), complex (mnng + r.a.k.), and blank control (c). in mnng group, mnng (250 micrograms) was injected intravenously once five days for seven times in each mouse, the total dosage of mnng was 1.75 mg ...19911748413
influences of refined konjac meal on the levels of tissue lipids and the absorption of four minerals in rats.this paper reports a study on the hypocholesterolemic effect of the refined konjac meal (rkm) containing about 80% glucomannan prepared from the tubers of amorphophallus konjac k. koch. male and female sprague-dawley rats, 5 weeks old, were divided into five groups and fed a normal basal diet, a hypercholesterolemic diet (control diet), and three test diets (rkm added to the control diet at levels of 2.5, 5, and 10%, respectively) for 12 weeks. the results obtained indicate that rkm could marked ...19902252550
[immunochemical study on the reaginic antibody in konnyaku asthma]. 19694186279
[study on the allergens of asthma caused by amorpholus konnyaku]. 19665977610
[influence of refined amorphophallus konjac on osteoporosis in the aged female rats: a preliminary bone histomorphometric analysis].the hypolipidaemic and antisteatotic effects of refined amorphophallus konjac (rak) have been reported. in order to further evaluate the effect of rak on osteoporosis in the aged female sd rats, forty one month aged female sd rats were divided into three groups: a normal diet group (s), another normal diet group (a) and a test group (b) in which the animals were fed on a diet similar to that of the normal diet group with the addition of rak at a dosage of 1%. at the end of the sixth month of the ...19947896258
degradation of konjac glucomannan by enzymes in human feces and formation of short-chain fatty acids by intestinal anaerobic bacteria.konjac (konnyaku) glucomannan was examined for its degradation in human intestines and fermentation products. the konjac glucomannan was degraded almost 100% by soluble enzymes in human feces to give 4-o-beta-d-mannopyranosyl-d-mannopyranose (beta-1,4-d-mannobiose), 4-o-beta-d-glucopyranosyl-d-glucopyranose (cellobiose), 4-o-beta-d-glucopyranosyl-d-mannopyranose, and small amounts of glucose and mannose. these three disaccharides were further degraded by a cell-associated enzyme(s) to glucose or ...19989742462
hypersensitivity pneumonitis induced by konjak flour and powdered hijikia fusiforme.this is a report of a patient with occupational hypersensitivity pneumonitis (hp) caused by konjak flour and powdered hijikia fusiforme. a 56-year-old man had worked as a konnyaku manufacturer for 38 years, and suffered from dyspnea on exertion. konnyaku is a paste made from konjak flour, powdered hijikia fusiforme, quicklime, and powdered scallop shell. the diagnosis of hp was confirmed immunologically by the detection of serum precipitins to powdered hijikia fusiforme, and by the positive resu ...200314518674
[glucomannan: properties and therapeutic applications].glucomannan is a dietary fiber employed quite frequently in the western countries since two decades now, as its ingestion plays an important role in human health. however, eastern people have used this fiber for more than a thousand years. this dietary fiber is the main polysaccharide obtain from the tubers of the amorphophallus konjac plant, a member of the family araceae found in east asia. the chemical structure of glucomannan consists, mainly, in mannose and glucose in the ratio 8:5 linked b ...200414983741
[molecular identification and genetic diversity in konnyaku's soft rot bacteria].the soft rot bacteria erwinia are an important pathogens of konnyaku and other ornamental plants. thirty-three strains were isolated from soft-rotted konnyaku and other ornamental plants. according to the characteristics of pathogenicity and culture character on semi selective medium (crystal violet pectate, cvp), most of strains tested caused rotten symptom in tubers and stems of konnyaku, and characteristic deep cavities were formed on cvp. to amplify 16s-23s rdna intergenic transcribed spacer ...200617037047
inhibitory effects of a soluble dietary fiber from amorphophallus konjac on cytotoxicity and dna damage induced by fecal water in caco-2 cells.the aims of this study were to determine the effects of konjac glucomannan (kgm), a water-soluble dietary fiber from amorphophallu konjac c. koch, on the cytotoxicity and dna damage of fecal water-treated caco-2 cells, a human colon adenocarcinoma cell line, and to compare these effects with those of inulin, oligofructose (fo), cellulose and no fiber diet. in addition, the possible mechanisms by which dietary fibers modulated the toxicity of feces were investigated. seven-week-old balb/c mice we ...200717893827
[isolation of endophytic antagonistic bacterium from amorphophallus konjac and research on its antibacterial metabolite].an endophytic antagonistic bacterium was isolated from amorphophallus konjac calli. in order to identify this bacterium, 16s rdna was amplified and partially sequenced. sequence comparison showed that this sequence has the highest similarity to that in bacillus subtilis, with 99.0% identities. that demonstrated this bacterium belongs to bacillus subtili , named bsn5. the extracted extracellular protein from strain bsn5 had antibacterial activity against erwinia carotovora subp. carotovora, which ...200718271267
transgenic amorphophallus konjac expressing synthesized acyl-homoserine lactonase (aiia) gene exhibit enhanced resistance to soft rot disease.amorphophallus konjac is an important economic crop widely used in health products and biomaterials. however, this monocotyledonous plant's production is seriously restricted by soft rot disease. some bacillus thuringiensis strains generate an endocellular acyl homoserine lactonase (aiia), which has inhibitory effect on soft rot pathogen through disrupting the signal molecules (n-acylhomoserine lactones, ahl) of their quorum sensing system. the aim of our study is to obtain transgenic a. konjac ...200919898849
chemically modified konjac glucomannan with high colloid osmotic pressure: physiological evaluation in a rabbit model as a plasma substitute.carboxylmethylated konjac glucomannan (ckgm) is a carboxylmethylated polymer of mannose and glucose that is derived from the plant amorphophallus konjac cultivated in east asia. the ckgm solution had a high volume-expanding efficacy and was evaluated as a plasma substitute in the present study. ameliorative hemorrhagic shock rabbits were used as the model animals. the in vivo hemodynamic and hemorheologic properties, including blood pressure, blood viscosity, hematocrit, erythrocyte deformation ...201020466646
complete genome sequence of bacillus subtilis bsn5, an endophytic bacterium of amorphophallus konjac with antimicrobial activity for the plant pathogen erwinia carotovora subsp. carotovora.here, we present the complete genome sequence of bacillus subtilis strain bsn5, isolated from amorphophallus konjac calli tissue and showing strong inhibitory activity to erwinia carotovora subsp. carotovora, which causes amorphophallus soft rot disease and affects the industry development of this organism.201121317323
comparison of konjac glucomannan digestibility and fermentability with other dietary fibers in vitro.abstract konjac glucomannan (kgm) is a dietary fiber found in amophophallus konjac. this fiber is fermentable based on human and animal trials, but short-chain fatty acid (scfa) production profiles are unknown. the aim of this study is to characterize the digestibility and fermentability in vitro of two preparations of kgm, to better understand how kgm improves human health. konnyaku (yam cake made of a. konjac), isolated kgm, inulin, and guar gum were subjected to in vitro digestion and in vi ...201122149628
an β-1,4-xylanase with exo-enzyme activity produced by paenibacillus xylanilyticus kj-03 and its cloning and characterization.paenibacillus xylanilyticus kj-03 was isolated from soil samples obtained from a field with amorphophallus konjac plants. a gene encoding xylanase was isolated from kj-03 and cloned using a fosmid library. the xyna gene encodes xylanase; it consists of 1,035 bp and encodes 345 amino acids. the amino acid sequence deduced from the p. xylanilyticus kj-03 xylanase showed 81% and 69% identities with those deduced from the p. polymyxa e681 and paenibacillus sp. hpl-001 xylanases, respectively. the xy ...201323462014
gene cloning of endoglucanase cel5a from cellulose-degrading paenibacillus xylanilyticus kj-03 and purification and characterization of the recombinant enzyme.the bacterial strain paenibacillus xylanilyticus kj-03 was isolated from a sample of soil used for cultivating amorphophallus konjac. the cellulase gene, cel5a was cloned using fosmid library and expressed in escherichia coli bl21 (trxb). the cel5a gene consists of a 1,743 bp open reading frame and encodes 581 amino acids of a protein. cel5a contains n-terminal signal peptide, a catalytic domain of glycosyl hydrolase family 5, and duf291 domain with unknown function. the recombinant cellulase wa ...201222350314
cyclodextrin glycosyltransferase encoded by a gene of paenibacillus azotofixans yupp-5 exhibited a new function to hydrolyze polysaccharides with β-1,4 linkage.the bacteria with hydrolysis activity to glucomannan were isolated from the rhizosphere of amorphophallus konjac through enrichment cultivation. one strain with strong activity in degrading glucomannan was identified preliminarily as paenibacillus azotofixans yupp-5 according to the sequence analysis of 16s rdna. this strain is able to hydrolyze many polysaccharide with β-1,4 linkage, including glucomannan, galactomannan, xylan, carboxymethyl cellulose, and chitin. one hydrolytic enzyme band of ...201222226202
konjac glucomannan, a promising polysaccharide of amorphophallus konjac k. koch in health care.in recent year, konjac glucomannan (kgm) has attracted more attention due to its non-harmful and non-toxic properties, good biocompatibility, biodegradability and hydrophilic ability. moreover, kgm and their derivatives have several importances in the multidirectional research areas such as nutritional, biotechnological and fine chemical fields. in the previous article, we have reviewed the nutritional aspects of kgm covering the various aspects of functional foods, food additives and their deri ...201627481345
devil's tongue weed (grateloupia turuturu yamada) in northern portugal: passenger or driver of change in native biodiversity?understanding the mechanisms underlying biological invasions is essential to separate their actual ecological effects from those of other human disturbances. this study examined experimentally whether the non-native red seaweed grateloupia turuturu is an opportunistic species taking advantage of degraded local conditions (passenger model), or the primary driver of changes in the structure of benthic assemblages (driver model). in a first experiment, traits of g. turuturu likely associated to its ...201627110653
genetic variation in wild populations of the tuber crop amorphophallus konjac (araceae) in central china as revealed by aflp markers.amorphophallus konjac is an economically important crop. in order to provide baseline information for sustainable development and conservation of the wild plant resources of a. konjac, we studied the genetic diversity and population structure of this species using amplified fragment length polymorphism (aflp) molecular markers. we sampled 139 individuals from 10 wild populations of a. konjac in central china. using five aflp primer combinations, we scored a total of 270 dna fragments, most of wh ...201526782525
beneficial health characteristics of native and hydrolysed konjac (amorphophallus konjac) glucomannan.the impact of ingesting glucomannans on health is not limited to colonic-focused fermentation into short-chain fatty acids (scfas), which might have some local health benefits; it also helps in treating disease states and enhancing the body's immune system, both within the gut and in/on other parts of the body. the local and systemic roles of hydrolysed glucomannans, especially konjac glucomannans, in the mouth, oesophagus, stomach, small intestine, large intestine, gut-associated lymphoid tissu ...201626676961
mimicking livor mortis: a well-known but unsubstantiated color profile in sapromyiophily.by emitting strong scents resembling rotting organic materials suitable for oviposition and/or foraging of flies, sapromyiophilous flowers mimic the substrates that attract flies as pollinators. it has been suggested that the wide range of volatile organic compounds emitted by this deceptive pollination system reflects the trophic preferences of flies to different types of substrate, including herbivore and carnivore feces, carrion, and fruiting bodies of fungi. previous studies suggest that flo ...201526306591
effect of glucomannan supplementation on body weight in overweight and obese children: protocol of a randomised controlled trial.glucomannan (gnn), a water-soluble dietary fibre derived from the plant amorphophallus konjac, is marketed for weight reduction. the exact mechanisms by which gnn might exert its actions are unclear. however, it has been shown that gnn slows gastric emptying by forming a viscous gel of large volume, which increases the feeling of satiety. current evidence on the effectiveness of gnn for weight reduction is sparse, and well-designed trials performed in children are needed to assess the efficacy o ...201525869689
the effect of glucomannan on body weight in overweight or obese children and adults: a systematic review of randomized controlled trials.glucomannan (gm), a soluble fiber derived from the plant amorphophallus konjac, is marketed as being helpful in reducing body weight. however, the data supporting this claim are scarce. the aim of this review was to systematically evaluate the effects of gm on body weight (bw) and body mass index (bmi) in otherwise healthy obese or overweight children and adults.201525701331
use of the konnyaku shirataki noodle as a low fidelity simulation training model for microvascular surgery in the operating theatre.the aim of this study was to test the feasibility of a type of japanese noodle, named "shirataki konnyaku", for microsurgery training in the operating room. thirteen surgical residents without experience in microsurgery had to perform two microsurgical anastomoses: one on a model of a femoral artery of a rat (control) and one on a model of a konnyaku shirataki. two quantitative variables (time in minutes and number of stitches to perform the anastomosis) and two qualitative variables (patency an ...201424603055
development of microsatellite markers by transcriptome sequencing in two species of amorphophallus (araceae).amorphophallus is a genus of perennial plants widely distributed in the tropics or subtropics of west africa and south asia. its corms contain a high level of water-soluble glucomannan; therefore, it has long been used as a medicinal herb and food source. genetic studies of amorphophallus have been hindered by a lack of genetic markers. a large number of molecular markers are required for genetic diversity study and improving disease resistance in amorphophallus. here, we report large scale of t ...201323870214
identification of chloroplast genome loci suitable for high-resolution phylogeographic studies of colocasia esculenta (l.) schott (araceae) and closely related taxa.recently, we reported the chloroplast genome-wide association of oligonucleotide repeats, indels and nucleotide substitutions in aroid chloroplast genomes. we hypothesized that the distribution of oligonucleotide repeat sequences in a single representative genome can be used to identify mutational hotspots and loci suitable for population genetic, phylogenetic and phylogeographic studies. using information on the location of oligonucleotide repeats in the chloroplast genome of taro (colocasia es ...201323718317
high molecular weight plant heteropolysaccharides stimulate fibroblasts but inhibit keratinocytes.konjac glucomannan (kgm) is a natural polysaccharide of β(1-4)-d-glucomannopyranosyl backbone of d-mannose and d-glucose derived from the tuber of amorphophallus konjac c. koch. kgm has been reported to have a wide range of activities including wound healing. in this study we examined kgm extracts prepared from five plant species, (amorphophallus konjac koch, amorphophallus oncophyllus, amorphophallus prainii, amorphophallus paeoniifolius and amorphophallus elegans) for their effects on cultured ...201323694709
temporal and spatial regulation of glucomannan deposition and mobilization in corms of amorphophallus konjac (araceae).konjac glucomannan (kgm), the main biologically active constituent of konjac flour extracted from corms of amorphophallus konjac (konjac), has potential to be used as a nutraceutical (satiety agent) to combat obesity. here we present the results of an immunocytochemical investigation of the developmental regulation of the deposition and mobilization of glucomannan in corm tissues of konjac, using an antiheteromannan (mannan/glucomannan) antiserum.201323347975
isolation and characterization of microsatellite loci for the herbaceous tuber crop, amorphophallus konjac (araceae).amorphophallus konjac is an herbaceous tuber crop with tremendous potential for commercial development. we report the development of microsatellite primers for this important crop species. thirteen polymorphic microsatellite markers were developed and tested in two populations of a. konjac from the wuling mountain region (wl population) and the yunnan province (yn population) in china. the number of alleles per locus ranged from 1 to 7; the observed and expected heterozygosities ranged from 0 to ...201223096926
deep sequencing of voodoo lily (amorphophallus konjac): an approach to identify relevant genes involved in the synthesis of the hemicellulose glucomannan.a roche 454 cdna deep sequencing experiment was performed on a developing corm of amorphophallus konjac--also known as voodoo lily. the dominant storage polymer in the corm of this plant is the polysaccharide glucomannan, a hemicellulose known to exist in the cell walls of higher plants and a major component of plant biomass derived from softwoods. a total of 246 mega base pairs of sequence data was obtained from which 4,513 distinct contigs were assembled. within this voodoo lily expressed sequ ...201121538106
[extraction and purification method of rice dna from rice powder containing konjak flour].rice powder containing konjak flour made with tuberous roots of amorphophallus konjac is imported as a rice-processed product from china to japan. an improved dna purification method for the polymerase chain reaction (pcr) analysis of rice in such products is necessary, since konjak flour constituents absorb the dna purification buffer to form a gel, and cause problems in the subsequent purification steps. here, we present a simple preparative system for isolation of the rice and a purification ...201021071909
traditional uses and potential health benefits of amorphophallus konjac k. koch ex n.e.br.amorphophallus konjac (konjac) has long been used in china, japan and south east asia as a food source and as a traditional medicine. flour extracted from the corm of this species is used in far eastern cuisine to make noodles, tofu and snacks. in traditional chinese medicine (tcm), a gel prepared from the flour has been used for detoxification, tumour-suppression, blood stasis alleviation and phlegm liquefaction; and for more than 2000 years has been consumed by the indigenous people of china f ...201020079822
a cell-block preparation using glucomannan extracted from amorphophallus konjac.to evaluate a cell-block preparation using glucomannan, which was extracted from amorphophallus konjac. ten specimens were centrifuged at 1,500 rpm for 5 minutes, the supernatant was removed; the remnant after the preparation of smear specimens for routine cytological examination was fixed with 20% formalin. the specimen was recentrifuged at 1,500 rpm for 5 minutes, and the supernatant was removed. the residue was resuspended with 2 ml of eosin solution and 1-5 ml of 80% alcohol, and stirred wel ...201019941364
evaluation of the pharmacotherapeutic efficacy of garcinia cambogia plus amorphophallus konjac for the treatment of obesity.hydroxycitric acid (hca), the main compound of garcinia cambogia extract, is a competitive blocker of atp-citrate-lyase, presenting a potential inhibition of fatty acid biosynthesis. glucomannan fibers, abundant in amorphophallus konjac, seem to reduce the absorption kinetics of dietary fat. therefore, the aim of this double-blind randomized study was to evaluate the pharmacotherapeutic efficacy of standardized extracts of g. cambogia (52.4% hca) plus a. konjac (94.9% glucomannan) in the treatme ...200818729243
[relationship of resistance to diseases and water-soluble amino acids in konjac leaves].reversed-phase high performance liquid chromatography was used to analyze water-soluble amino acids in the normal amorphophallus konjac, amorphophallus albus, amorphophallus bulbifer, and the soft rot amorphophallus konjac, to determine the relationship of the different soft-rot resistant konjac varieties and the proportion and content of the multiple water-soluble amino acids. the results showed that there are remarkable differences in the content and proportion of water-soluble amino acids in ...200818724679
esophageal obstruction from a hygroscopic pharmacobezoar containing glucomannan.glucomannan is a dietary fiber that is the main polysaccharide obtained from the tubers of the amorphophallus konjac plant. it has been used as a dietary fiber for more than 1,000 years in eastern cultures, and has gained popularity in many western countries over the last 20 years. this soluble fiber has very substantial water-holding properties, and forms highly viscous solutions when dissolved in water. it also has considerable hygroscopic properties, expanding rapidly to many times the size o ...200717357389
glucomannan and obesity: a critical review.glucomannan (gm) is a soluble, fermentable, and highly viscous dietary fiber derived from the root of the elephant yam or konjac plant, which is native to asia. preliminary evidence suggests that gm may promote weight loss. this review summarizes studies using gm for weight loss as well as studies investigating its mechanisms of action. at doses of 2-4 g per day, gm was well-tolerated and resulted in significant weight loss in overweight and obese individuals. there is some evidence that gm exer ...201616320857
molecular cloning and characterization of a novel mannose-binding lectin gene from amorphophallus konjac.a new lectin gene was cloned from amorphophallus konjac. the full-length cdna of amorphophallus konjac agglutinin (aka) was 736 bp and contained a 474 bp open reading frame encoding a 158 amino acid protein. homology analysis revealed that the lectin from this araceae species belonged to the superfamily of monocot mannose-binding proteins. molecular modeling of aka indicated that the three-dimensional structure of aka strongly resembles that of the snowdrop lectin. southern blot analysis of the ...200312974473
structure of the oligomers obtained by enzymatic hydrolysis of the glucomannan produced by the plant amorphophallus konjac.dimers and trimers obtained by enzymatic hydrolysis of the glucomannan produced by the plant amorphophallus konjac were analysed in order to obtain information on the saccharidic sequences present in the polymer. the polysaccharide was digested with cellulase and beta-mannanase and the oligomers produced were isolated by means of size-exclusion chromatography. they were structurally characterised using electrospray mass spectrometry, capillary electrophoresis, and nmr. the investigation revealed ...200212493237
effect of amorphophallus konjac oligosaccharides on stz-induced diabetes model of isolated islets.three oligosaccharide fractions from the root of amorphophallus konjac, which was reported with hypoglycemic effects on diabetes subjects, were isolated and studied using the stz-treated diabetes model. among them, one fraction named as kos-a, was found with nitric oxide (no(*)) free radical regulation effect, while the other two were not. at concentrations less than 1.5 mm, kos-a positively decreased stz-induced no(*) level of islets, but normal no(*) release for non-stz-treated islets was not ...200212467911
[colorimetric determination of sulfite in "kanpyo" (dried gourd shavings) and "konnyakuseiko" (devil's-tongue fine powder) using sulfite oxidase and catalase].a simple and convenient method for colorimetric determination of sulfite in foods based on its conversion to formaldehyde with sulfite oxidase and catalase was developed. sulfite in a sample was extracted with water and then diluted with methanol. one ml of sample solution containing about 5-10 micrograms of sulfite was taken into a test tube with a ground-glass stopper, and 3 ml of 0.04 mol/l borate buffer (ph 8.7), 1 ml of 0.4% 3-methyl-2-benzothiazolinone hydrazone (mbth) solution, 2,000 unit ...200212238155
peroxynitrite scavenging activities of aromatic compounds isolated from konnyaku, amorphophallus konjac k.koch.(+/-)-5,5'-dimethoxysesamin, erythrinasinate, indole-3-carbaldehyde, (7r,8s)-dihydrodehydrodiconiferyl alcohol 9-o-beta-d-glucopyranoside, cis- and trans-n-(p-coumaroyl)serotonin, serotonin, 3,4-dihydroxybenzoic acid, and 3,4-dihydroxybenzaldehyde have been found in tobiko, a food by-product, and evaluation of their peroxynitrite scavenging activities has been done. among these compounds, serotonin, trans-n-(p-coumaroyl)serotonin, 3,4-dihydroxybenzaldehyde, and 3,4-dihydroxybenzoic acid showed s ...200212162564
cis-n-(p-coumaroyl)serotonin from konnyaku, amorphophallus konjac k. koch.tobiko is produced as a by-product in the manufacture of konnyaku and comes to about 45% of dried konnyaku-imo. for a possible use of this powder, we studied its components. serotonin, trans-n-(p-coumaroyl) serotonin, and a new compound, cis-n-(p-coumaroyl)serotonin, were characterized from this powder.200011129612
use of glucomannan for the separation of dna fragments by capillary electrophoresis.glucomannan, a natural polysaccharide extracted from amorphophallus konjac, was employed as a sieving additive for the separation of restriction dna fragments in capillary electrophoresis. a stable solution of entangled glucomannan was successfully prepared under mild conditions. the separation capillary filled with 0.25% glucomannan exhibited excellent resolution and reproducibility on separation of dna fragments over the range of ca. 1400 base pairs. the separation was dependent on the size of ...19938281262
amplexus induces surge of luteinizing hormone in male toads, bufo japonicus.at mating time in the wild, male toads (bufo japonicus) exhibit a surge in circulating luteinizing hormone (lh) that is thought to result in spermiation. experiments were performed to identify proximate control of this gonadotropin surge. male toads migrating to a breeding pond were captured and put in plastic containers either with equal numbers of females or without females. all the males placed with the females clasped them in amplexus that lasted for about 12 hr. during this period, plasma l ...19921505728
[gastrointestinal transit time (gitt) in normal chinese and patients].two kinds of radiopaque pellets were ingested as markers to determine gitt in 60 normal subjects, 7 patients with ulcerative colitis (uc), 10 patients with idiopathic constipation (ic) and 8 patients with other diseases. the food contained 10-20g dietary fiber per day. besides, gitt was determined in 14 normal subjects whose dietary content was 40-50g or 10g mo yu and 10-20g dietary fiber per day. results are expressed in hours as 50% transit time (mean +/- s) and the values or normal subjects a ...19901965498
effect of food intake on urinary excretions of histamine, n tau-methylhistamine, imidazole acetic acid and its conjugate(s) in humans and mice.the urinary excretions by young healthy men of histamine and its metabolites, n tau-methylhistamine, imidazole acetic acid, and imidazole acetic acid conjugate(s), increased 1-3 h after food intake. the increase was seen even after the intake of konnyaku (mannan) as a protein-deficient food, suggesting that physical stimulation of the gastric mucosa by food is the main cause of histamine release. this suggestion was confirmed by the following findings in patients and mice. in patients with stoma ...19846442293
elevated atmospheric partial pressure of carbon dioxide and dry matter production of konjak (amorphophallus konjac k. koch).konjak (amorphophallus konjac k. koch) was grown under normal (350 μbar) or enriched (700 μbar) co2 partial pressure in glasshouses kept at 33/26°c. doubling the co2 partial pressure resulted in twice the yield of corm because the net co2 assimilation rate doubled and, due to the simple source-sink relationship, the increased production was partitioned to the corm. the response to co2 of assimilation by konjak is discussed in relation to its original habitat in the tropics.198324458526
elevated atmospheric partial pressure of carbon dioxide and dry matter production of konjak (amorphophallus konjac k. koch).konjak (amorphophallus konjac k. koch) was grown under normal (350 μbar) or enriched (700 μbar) co2 partial pressure in glasshouses kept at 33/26 °c. doubling the co2 partial pressure resulted in twice the yield of corm because the net co2 assimilation rate doubled and, due to the simple source-sink relationship, the increased production was partitioned to the corm. the response to co2 of assimilation by konjak is discussed in relation to its original habitat in the tropics.198324458413
[physiological studies on konnyaku. part 1. on the nutritive value of konnyaku]. 19761036210
augmented serum insulin response to glucose infusion after the ingestion of konnyaku. 19715560675
[2 cases of konjac bronchial asthma found outside the "konnyaku" producing district]. 19695771765
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