| identification of begomoviruses infecting crops and weeds in belize. | plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in belize over a three year period with the aim of determining the diversity of the begomoviruses present. sixty five percent of the samples screened via dna hybridization produced signals indicative of begomovirus infection. subsequent pcr amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in belize. pepper gol ... | 2008 | 20596296 |
| next-generation sequencing and genome editing in plant virology. | next-generation sequencing (ngs) has been applied to plant virology since 2009. ngs provides highly efficient, rapid, low cost dna, or rna high-throughput sequencing of the genomes of plant viruses and viroids and of the specific small rnas generated during the infection process. these small rnas, which cover frequently the whole genome of the infectious agent, are 21-24 nt long and are known as vsrnas for viruses and vd-srnas for viroids. ngs has been used in a number of studies in plant virolo ... | 2016 | 27617007 |
| characterization and distribution of tomato yellow margin leaf curl virus, a begomovirus from venezuela. | a begomovirus causing mottling and leaf deformation in tomato from the state of mérida was cloned and sequenced. the virus has a bipartite genome comprised of a dna-a (2,572 nucleotides) and a dna-b (2,543 nucleotides) with a genome organization typical of new world begomoviruses. both components share a common region of 115 nucleotides with 98 % sequence identity. phylogenetic analysis indicated that while no virus sequences were closely related, the a component was distantly related to those o ... | 2013 | 23064695 |
| complete genome sequence of jacquemontia yellow mosaic virus, a novel begomovirus from venezuela related to other new world bipartite begomoviruses infecting convolvulaceae. | the complete genome of a bipartite begomovirus (genus begomovirus, family geminiviridae) infecting jacquemontia sp. (convolvulaceae) in venezuela has been cloned and sequenced. sequence comparison and phylogenetic analysis have shown that it represents an isolate of a novel species with closest relatives being two new world bipartite begomoviruses that infect convolvulaceae, jacquemontia mosaic yucatan virus and merremia mosaic virus. the dna-as of these begomoviruses, however, share only 77.0-7 ... | 2014 | 24463954 |