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preliminary identification and coat protein gene phylogenetic relationships of begomoviruses associated with native flora and cultivated plants from the yucatan peninsula of mexico.a number of native and cultivated eudicots in the yucatan peninsula of mexico (ypm) exhibit symptoms associated with virus infection. symptomatic leaves were collected and assessed for begomoviral detection using polymerase chain reaction (pcr), and universal primers that amplify a fragment of the coat protein gene (core cp). begomovirus were detected in nine native and seven cultivated species, representing seven eudicot families. dna extracts from the 16 hosts were used for pcr amplification a ...200717682933
expression of genes involved in the salicylic acid pathway in type h1 thioredoxin transiently silenced pepper plants during a begomovirus compatible interaction.the type-h thioredoxins (trxs) play a fundamental role in oxidative stress tolerance and defense responses against pathogens. in pepper plants, type-h trxs participate in the defense mechanism against cucumber mosaic virus. the goal of this study was to analyze the role of the catrxh1-cicy gene in pepper plants during compatible interaction with a dna virus, the euphorbia mosaic virus-yucatan peninsula (eumv-yp). the effects of a transient silencing of the catrxh1-cicy gene in pepper plants wëre ...201626606929
somatic homologous recombination in plants is promoted by a geminivirus in a tissue-selective manner.four transgenic arabidopsis thaliana lines carrying different reporter gene constructs based on split glucuronidase genes were used to monitor the frequency of somatic homologous recombination after geminivirus infections. euphorbia mosaic virus and cleome leaf crumple virus were chosen as examples, because they induce only mild symptoms and are expected to induce less general stress responses than other geminiviruses. after comparing the different plant lines and viruses as well as optimizing t ...201424606706
a new virus-induced gene silencing vector based on euphorbia mosaic virus-yucatan peninsula for npr1 silencing in nicotiana benthamiana and capsicum annuum var. anaheim.virus-induced gene silencing is based on the sequence-specific degradation of rna. here, a gene silencing vector derived from eumv-yp, named peumv-yp:╬┤av1, was used to silence chli and npr1 genes in nicotiana benthamiana. the silencing of the chli transcripts was efficient in the stems, petioles and leaves as reflected in tissue bleaching and reduced transcript levels. the silencing was stable, reaching the flowers and fruits, and was observed throughout the life cycle of the plants. additionall ...201323546940
genome sequence of euphorbia mosaic virus from passionfruit and euphorbia heterophylla in florida.euphorbia mosaic virus (eumv) was found in a symptomatic passionfruit (passiflora edulis) plant from homestead, florida, usa, as well as in the symptomatic weed euphorbia heterophylla this is the first identification of eumv in florida and the united states and the first report of a natural infection of passionfruit by eumv.201728254981
Begomoviruses infecting weeds in Cuba: increased host range and a novel virus infecting Sida rhombifolia.As a result of surveys conducted during the last few years to search for wild reservoirs of begomoviruses in Cuba, we detected a novel bipartite begomovirus, sida yellow mottle virus (SiYMoV), infecting Sida rhombifolia plants. The complete genome sequence was obtained, showing that DNA-A was 2622 nucleotides (nt) in length and that it was most closely related (87.6% nucleotide identity) to DNA-A of an isolate of sida golden mosaic virus (SiGMV) that infects snap beans (Phaseolus vulgaris) in Fl ...201121964921
analysis of a new strain of euphorbia mosaic virus with distinct replication specificity unveils a lineage of begomoviruses with short rep sequences in the dna-b intergenic region.euphorbia mosaic virus (eumv) is a member of the slcv clade, a lineage of new world begomoviruses that display distinctive features in their replication-associated protein (rep) and virion-strand replication origin. the first entirely characterized eumv isolate is native from yucatan peninsula, mexico; subsequently, eumv was detected in weeds and pepper plants from another region of mexico, and partial dna-a sequences revealed significant differences in their putative replication specificity det ...201020958988
identification of begomoviruses infecting crops and weeds in belize.plants including pepper, red kidney bean, squash, string bean and tomato, as well as weeds with viral symptoms were collected from five districts in belize over a three year period with the aim of determining the diversity of the begomoviruses present. sixty five percent of the samples screened via dna hybridization produced signals indicative of begomovirus infection. subsequent pcr amplifications and nucleotide sequence analyses revealed the presence of four begomoviruses in belize. pepper gol ...200820596296
the first dna 1-like alpha satellites in association with new world begomoviruses in natural infections.from brazilian weeds with typical symptoms of a geminivirus infection, the dnas of two new virus species, two new strains with two variants of already known bipartite begomoviruses were sequenced. moreover, the first two dna 1-like satellites (alpha satellites) occurring naturally in the new world were identified. they are related to nanoviral dna components and show a typical genome organization with one open reading frame coding potentially for a replication-associated protein (rep), a conserv ...201020553707
complete nucleotide sequence of an isolate of euphorbia mosaic virus that infects euphorbia heterophylla and wissadula amplissima in jamaica. 200919774336
molecular and biological characterization of a new brazilian begomovirus, euphorbia yellow mosaic virus (euymv), infecting euphorbia heterophylla plants.to date, no begomovirus has been fully characterized from euphorbia heterophylla, a widely distributed weed, in brazil. here, we show the occurrence of a new begomovirus on e. heterophylla plants showing bright yellow mosaic. the bipartite viral genome was cloned from 10 samples, and all clones are almost identical to each other (95.6-98.8% nucleotide sequence identity). the dna-a sequences shared a maximum nucleotide sequence identity of 87.3% with euphorbia mosaic peru virus (eumpv) and thus w ...201121779908
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