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molecular diagnosis of group b coltiviruses infections.the group-b of genus coltivirus encompasses isolates from humans, ticks or mosquitoes collected in indonesia and china. subgroup-b1 includes the strain jkt/dsr-7075 and subgroup-b2 strains jkt/dsr-6423, jkt/dsr-6969, jkt/dsr-7043 and the banna virus. data are described for the pcr-based diagnosis of infection by group b coltiviruses. sets of primers were designed from the sequences of the 7th, 9th and 12th viral segments and rt pcr assays were developed. consensus primers permitted the detection ...199910488759
complete sequence determination and genetic analysis of banna virus and kadipiro virus: proposal for assignment to a new genus (seadornavirus) within the family reoviridae.arboviruses with genomes composed of 12 segments of double-stranded (ds) rna have previously been classified as members or probable members of the genus coltivirus within the family reoviridae: a number of these viruses have been isolated in north america and europe and are serologically and genetically related to colorado tick fever virus, the coltivirus type species. these isolates constitute subgroup a of the coltiviruses. the complete genome sequences are now presented of two asian arbovirus ...200010811934
recombinant vp9-based enzyme-linked immunosorbent assay for detection of immunoglobulin g antibodies to banna virus (genus seadornavirus).banna virus (bav, genus seadornavirus, family reoviridae) is an arbovirus suspected to be responsible for encephalitis in humans. two genotypes of this virus are distinguishable: a (chinese isolate, bav-ch) and b (indonesian isolate, bav-in6969) which exhibit only 41% amino-acid identity in the sequence of their vp9. the vp7 to vp12 of bav-ch and vp9 of bav-in6969 were expressed in bacteria using pgex-4t-2 vector. vp9 was chosen to establish an elisa for bav, based mainly on two observations: (i ...200414715307
the structure and function of the outer coat protein vp9 of banna virus.banna virus (bav: genus seadornavirus, family reoviridae) has a double-shelled morphology similar to rotavirus and bluetongue virus. the structure of bav outer-capsid protein vp9 was determined by x-ray crystallography at 2.6 a resolution, revealing a trimeric molecule, held together by an n-terminal helical bundle, reminiscent of coiled-coil structures found in fusion-active proteins such as hiv gp41. the major domain of vp9 contains stacked beta sheets with marked structural similarities to th ...200515642258
identification and functional analysis of vp3, the guanylyltransferase of banna virus (genus seadornavirus, family reoviridae).banna virus (bav) particles contain seven structural proteins: vp4 and vp9 form an outer-capsid layer, whilst the virus core contains three major proteins (vp2, vp8 and vp10) and two minor proteins (vp1 and vp3). sequence analysis showed that vp3 contains motifs [kx(i/v/l)s] and (hx(n)h) that have previously been identified in the guanylyltransferases of other reoviruses. incubation of purified bav-ch core particles with [alpha-32p]gtp resulted in exclusive covalent labelling of vp3, demonstrati ...200515784908
structural organization of an encephalitic human isolate of banna virus (genus seadornavirus, family reoviridae).banna virus (bav) is the type species of the genus seadornavirus within the family reoviridae. the chinese bav isolate (bav-ch), which causes encephalitis in humans, was shown to have a structural organization and particle morphology reminiscent of that of rotaviruses, with fibre proteins projecting from the surface of the particle. intact bav-ch virus particles contain seven structural proteins, two of which (vp4 and vp9) form the outer coat. the inner (core) particles contain five additional p ...200515784909
coltiviruses and seadornaviruses in north america, europe, and asia.coltiviruses are tickborne viruses of the genus coltivirus. the type species, colorado tick fever virus (from north america), has been isolated from patients with flulike syndromes, meningitis, encephalitis, and other severe complications. another coltivirus, eyach virus, has been isolated from ticks in france and germany and incriminated in febrile illnesses and neurologic syndromes. seadornaviruses are endemic in southeast asia, particularly indonesia and china. the prototype virus of the genu ...200516318717
liao ning virus, a new chinese seadornavirus that replicates in transformed and embryonic mammalian cells.seadornaviruses are emerging arboviral pathogens from the south-east of asia. the genus seadornavirus contains two distinct species, banna virus (bav) isolated from humans with encephalitis and kadipiro virus. bav replicates within insect cells and mice but not in cultured mammalian cells. here, the discovery of liao ning virus (lnv), a new seadornavirus from the aedes dorsalis mosquito, which was completely sequenced and was found to be related to bav and kadipiro virus, is reported. two seroty ...200616361432
[detection of banna virus-specific nucleic acid with taqman rt-pcr assay].to develop a rapid and more sensitive taqman rt-pcr assay specific for banna virus.200616642219
isolation and molecular characterization of banna virus from mosquitoes, vietnam.we isolated and characterized a banna virus from mosquitoes in vietnam; 5 strains were isolated from field-caught mosquitoes at various locations; banna virus was previously isolated from encephalitis patients in yunnan, china, in 1987. together, these findings suggest widespread distribution of this virus throughout southeast asia.200818680655
distribution of arboviruses and mosquitoes in northwestern yunnan province, china.from july to september in 2005 and 2006, a survey was conducted to identify mosquito species and mosquito-borne arboviruses at elevations ranging from 900-3280 m between 24 degrees 00' n and 29 degrees 00' n latitude in the northwestern part of yunnan province, china. a total of 54,879 mosquitoes representing 15 species and 4 genera was collected using uv light traps at 59 sites. culex tritaeniorhynchus and anopheles sinensis were the most abundant species. the density of mosquitoes as well as t ...200919196130
[isolation and identification of arboviruses from mosquito pools in some regions of liaoning province, china].to isolate and identify arboviruses from mosquito pools in some regions of liaoning province.200919565849
metagenomic analysis of rna viruses in a fresh water lake.freshwater lakes and ponds present an ecological interface between humans and a variety of host organisms. they are a habitat for the larval stage of many insects and may serve as a medium for intraspecies and interspecies transmission of viruses such as avian influenza a virus. furthermore, freshwater bodies are already known repositories for disease-causing viruses such as norwalk virus, coxsackievirus, echovirus, and adenovirus. while rna virus populations have been studied in marine environm ...200919787045
[arbovirus investigation in some regions of shanxi province in 2007].to investigate arboviruses in some regions of shanxi province, isolation and identification for arbovirus activity from mosquitoes was conducted.200919799012
[isolation and identification of banna virus from mosquito for the first time in inner mongolia].to identify the virus isolated from a mosquito culex modestus collected from tongliao city of inner mongolia autonomous region.200920104749
[investigation on arboviruses at sino-vietnam border areas in wenshan of yunnan province].to investigate arboviruses in wenshan and hekou county which are the sino-vietnam frontier regions of wenshan, yunnan province, china.200920193505
banna virus, china, 1987-2007.banna viruses (bavs) have been isolated from pigs, cattle, ticks, mosquitoes, and human encephalitis patients. we isolated and analyzed 20 bavs newly isolated in china; this finding extends the distribution of bavs from tropical zone to north temperate climates and demonstrate regional variations in bav phylogeny and mosquito species possibly involved in bav transmission.201020202434
development of a rapid and comprehensive proteomics-based arboviruses detection system.a rapid and comprehensive protocol, which combines simple purification and liquid chromatography-electrospray ionisation-tandem mass spectrometry (lc-esi/ms/ms), was developed for identification of arboviruses in infected culture fluid. using this protocol, various arboviruses were detected including uncommon viruses that were described previously as banna virus and yunnan orbivirus. this approach is useful for the rapid screening of viral samples that cannot be identified by conventional gene a ...201020304016
the neglected arboviral infections in mainland china.the major arboviral diseases in mainland china include japanese encephalitis, dengue fever, crimean-congo hemorrhagic fever (also known as xinjiang hemorrhagic fever), and tick-borne encephalitis. these and other newly found arbovirus infections due to banna virus and tahyna virus contribute to a large and relatively neglected disease burden in china. here we briefly review the literature regarding these arboviral infections in mainland china with emphasis on their epidemiology, primary vectors, ...201020436960
[isolation and identification of a banna virus strain from mosquitoes in yunnan province.]objective: to isolate and identify banna virus (bav) from mosquitoes collected in mengla county of yunnan province. methods: mosquito samples were collected in houses and stock yards in mengla county, 2008. mosquitoes were homogenized and incubated onto both c6/36 and bhk21 cells. the new isolate was identified by using elisa and rt-pcr. the sequences of segment 5, 8 and 11 of bav were amplified by rt-pcr and determined. phylogenetic analysis on the new bav were performed using mega4 program. re ...201021163104
distribution of mosquitoes and mosquito-borne arboviruses in yunnan province near the china-myanmar-laos border.abstract. economic development and increased tourism in the southern region of yunnan province in china, adjacent to several countries in southeast asia, has increased the likelihood of import and export of vectors and vector-borne diseases. we report the results of surveillance of mosquitoes and mosquito-borne arboviruses along the border of china-myanmar-laos in 2005 and 2006, and information associating several arboviruses with infections and possibly disease in local human populations. seven ...201121540383
distribution of mosquitoes and mosquito-borne arboviruses in inner mongolia, china.during summers in 2007 and 2008, an investigation was conducted to identify the distribution of mosquitoes and circulation of mosquito-borne arboviruses in inner mongolia, china. a total of 10,542 mosquitoes consisting of seven species from the aedes, culex, and anopheles genera were trapped by uv-light traps, and they were sorted into 211 pools according to species, location, and date of collection. the result showed that aedes dorsalis was the most common species, accounting for 41.0% (4327/10 ...201121867416
enhanced arbovirus surveillance with deep sequencing: identification of novel rhabdoviruses and bunyaviruses in australian mosquitoes.viral metagenomics characterizes known and identifies unknown viruses based on sequence similarities to any previously sequenced viral genomes. a metagenomics approach was used to identify virus sequences in australian mosquitoes causing cytopathic effects in inoculated mammalian cell cultures. sequence comparisons revealed strains of liao ning virus (reovirus, seadornavirus), previously detected only in china, livestock-infecting stretch lagoon virus (reovirus, orbivirus), two novel dimarhabdov ...201324314645
isolates of liao ning virus from wild-caught mosquitoes in the xinjiang province of china in 2005.liao ning virus (lnv) is related to banna virus, a known human-pathogen present in south-east asia. both viruses belong to the genus seadornavirus, family reoviridae. lnv causes lethal haemorrhage in experimentally infected mice. twenty seven isolates of lnv were made from mosquitoes collected in different locations within the xinjiang province of north-western china during 2005. these mosquitoes were caught in the accommodation of human patients with febrile manifestations, or in animal barns w ...201222649554
isolation and genetic characterization of mangshi virus: a newly discovered seadornavirus of the reoviridae family found in yunnan province, china.seadornavirus is a genus of viruses in the family reoviridae, which consists of banna virus, kadipiro virus, and liao ning virus. banna virus is considered a potential pathogen for zoonotic diseases. here, we describe a newly discovered seadornavirus isolated from mosquitos (culex tritaeniorhynchus) in yunnan province, china, which is related to banna virus, and referred to as mangshi virus.201526630378
molecular evolution of emerging banna virus.banna virus (bav) is an emerging pathogen that causes human viral encephalitis and has been isolated from types of blood-sucking insects and mammals in asia. however, there are no reported systematic studies that describe the origin and evolution of bav. here, a phylogenetic analysis of bavs isolated from a variety of potential vectors and vertebrate hosts worldwide revealed that bavs emerged in the beginning of the 20th century and do not exhibit a species barrier. the mean substitution rate of ...201627590713
[analysis on molecular genetic evolution of banna virus based on the 12(th) segment].objective: to understand the evolution characteristics of banna viruses (bavs) isolated worldwide from 1980 to 2012. methods: in this study, a phylogenetic analysis using bayesian markov chain monte carlo simulations was conducted on all available 12(th) segment of genes of bavs isolated worldwide from 1980 to 2012 to investigate the evolutionary and epidemiologic dynamics of bavs. results: the bayesian phylogenetic analysis of bavs revealed that the common ancestor of bavs appeared 315 (95%hpd: ...201627655578
novel seadornavirus (family reoviridae) related to banna virus in europe.banna virus, whose genome is composed of 12 segments of double-stranded rna, is a member of the genus seadornavirus in the family reoviridae and is thought to be an emerging mosquito-transmitted human pathogen in southeast asia. a novel phylogenetic relative of banna virus (balaton virus, balv, jx947843-jx947850 and kc522611-kc522612) was identified using viral metagenomics in the intestinal contents of freshwater carp (cyprinus carpio) in hungary. the amino acid sequence identity of balaton vir ...201323624680
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