| pcr amplification of a specific double-stranded rna region of fiji disease virus from diseased sugarcane. | a 450-bp region from one species of the segmented dsrna genome of fiji disease virus (fdv) was amplified from total nucleic acid extracts of diseased plants by reverse transcription with mmlv, followed by amplification with taq dna polymerase (rt-pcr). other fdv-specific regions (c 150 bp and c 270 bp) were also amplified from the dsrna template. fdv cdna was only synthesised when the viral dsrna template was boiled and quenched with fdv-specific or random hexamer primers. the reverse transcript ... | 1992 | 1385465 |
| immunochemical detection of double-stranded ribonucleic acid in leaves of sugar cane infected with fiji disease virus. | | 1972 | 4622773 |
| similarity in the structure of cytoplasmic polyhedrosis virus, leafhopper a virus and fiji disease virus particles. | | 1982 | 6754656 |
| molecular analysis of fiji disease fijivirus genome segments 1 and 3. | fiji disease fijivirus (fdv) genomic segments 1 (s1) and 3 (s3) were completely sequenced. fdv s1 comprised 4,532 nt and was predicted to encode a 170.6 kda protein. fdv s3 comprised 3,623 nt and was predicted to encode a 135.5 kda protein. the terminal sequences of s1 and s3 were 5' aaguuuuu......cagcuagcguc 3' and 5' aaguuuuu......cagcagauguc 3', respectively, and located immediately adjacent to these sequences were 12 bp imperfect inverted repeats. the predicted translation product of fdv s1 ... | 2003 | 12876456 |
| molecular analysis of fiji disease virus segments 2, 4 and 7 completes the genome sequence. | the complete nucleotide sequences of fiji disease virus (fdv) genome segments s2, s4 and s7 were determined. this now completes the sequencing of all ten dsrna genome segments of the fijivirus type member, fdv, which comprises a total of 29339 nt. fdv s2, s4 and s7 comprised 3820, 3568 and 2194 nt, respectively. s2 and s4 each contained a single open reading frame (orf), which encoded putative proteins of 137 and 133 kda, respectively, while s7 contained two orfs, which encoded putative proteins ... | 2006 | 16525734 |
| molecular analysis of fiji disease virus genome segments 5, 6, 8 and 10. | the complete sequences of fiji disease virus (fdv) genome segments 5 (s5), s6, s8 and s10 were obtained and comprised 3150 nt, 2831 nt, 1959 nt and 1819 nt, respectively. each segment contained a single orf which encoded putative proteins of 115 kda, 97 kda, 69 kda and 63.0 kda, respectively. the putative amino acid sequences encoded by s5 and s6 contained putative leucine zipper motifs while fdv s5 and s8 each contained an atp-gtp-binding motif. at the amino acid level, fdv s5, s6, s8 and s10 s ... | 2004 | 15045559 |
| sequence analysis of the complete genome of rice black-streaked dwarf virus isolated from maize with rough dwarf disease. | the complete nucleotide sequences of 10 genomic segments (s1-s10) from an isolate of rice black-streaked dwarf virus causing rough dwarf disease on maize (rbsdv-hbm) in china were determined, a total of 29,142 base pairs (bp). each segment possessed the genus-specific termini with conserved nucleotide sequences of (+) 5'-aaguuuuu......cagcunnnguc-3' and a perfect or imperfect inverted repeat of seven to eleven nucleotides immediately adjacent to the terminal conserved sequence. while the coding ... | 2003 | 14501194 |
| resistance to fiji disease in sugar cane: role of cultivar preference by planthopper vector perkinsiella saccharicida (homoptera: delphacidae). | fiji disease (fd) of sugar cane caused by fiji disease virus (fdv) is transmitted by the planthopper perkinsiella saccharicida kirkaldy (hemiptera delphacidae). fd is effectively managed by using resistant cultivars, but whether the resistance is for the vector or for the virus is unknown. this knowledge would help develop a rapid and reliable glasshouse-based screening method for disease resistance. sugar cane cultivars resistant, intermediate, and susceptible to fd were screened in a glasshous ... | 2003 | 12650358 |
| sequence and phylogenetic analysis of genome segments s1, s2, s3 and s6 of mal de río cuarto virus, a newly accepted fijivirus species. | mal de río cuarto virus (mrcv) is a newly described species of the genus fijivirus, family reoviridae. the nucleotide sequence of four mrcv genome segments was determined. mrcv s1, s2, s3 and s6 were predicted to encode proteins of 168.4, 134.4, 141.7 and 90 kda, respectively. mrcv s1 encodes a basic protein that contains conserved rna-dependent rna polymerase motifs, and is homologous to rice black streaked dwarf virus (rbsdv), fiji disease virus (fdv) and nilaparvata lugens reovirus (nlrv) pol ... | 2003 | 12606083 |
| molecular characterisation of segments 1 to 6 of rice black-streaked dwarf virus from china provides the complete genome. | the nucleotide sequences of segments s1 to s6 of a chinese isolate of rice black-streaked dwarf virus (rbsdv) were determined. this provides the first complete sequence of a plant pathogenic member of the genus fijivirus. the complete ten-segment genome has 29,141 nucleotides, making it the largest reovirus genome so far reported. each of the segments s1-s6 is predicted to encode a single major protein. protein comparisons indicated that s1 encoded an rna dependent rna polymerase, with similarit ... | 2001 | 11811683 |
| molecular characterization of bombyx mori cytoplasmic polyhedrosis virus genome segment 4. | the complete nucleotide sequence of the genome segment 4 (s4) of bombyx mori cytoplasmic polyhedrosis virus (bmcpv) was determined. the 3,259-nucleotide sequence contains a single long open reading frame which spans nucleotides 14 to 3187 and which is predicted to encode a protein with a molecular mass of about 130 kda. western blot analysis showed that s4 encodes bmcpv protein vp3, which is one of the outer components of the bmcpv virion. sequence analysis of the deduced amino acid sequence of ... | 2001 | 11134312 |
| taxonomic implications for fijiviruses based on the terminal sequences of fiji disease fijivirus. brief report. | the 5' and 3' terminal sequences of the plus strand of fiji disease fijivirus (fdv) segments 2, 3, 9 and 10 possess the conserved terminal sequences, 5'aaguuuuu.....cagcagauguc 3'. the 5' sequence is identical to that of maize rough dwarf fijivirus (mrdv) and rice black-streaked dwarf fijivirus (rbsdv), whereas the fdv 3' sequence shares the consensus, cagcnnnnguc, with mrdv and rbsdv. the fdv terminal sequences, and the amino acid sequences from fdv segment 9, are more closely related to those ... | 1999 | 10603181 |
| molecular characterization of fiji disease fijivirus genome segment 9. | this is the first report of sequence from fiji disease fijivirus (fdv), the type member of the genus fijivirus of the family reoviridae. fdv genome segment (s9) comprised 1843 nt and contained two non-overlapping orfs, separated by a 57 nt intergenic region. s9 orf 1 comprised 1008 nt and encoded a 335-amino-acid polypeptide (predicted molecular mass 38.6 kda), while orf 2 comprised 627 nt and encoded a 208-amino-acid polypeptide (predicted molecular mass 23.8 kda). the 5' and 3' non-coding regi ... | 1998 | 9880035 |
| stability and extractability of double-stranded rna of pangola stunt and sugarcane fiji disease viruses in dried plant tissues. | when leaves infected with pangola stunt virus (pasv) were dried at 23, 37, 50, 70 or 105 degrees c, the dsrna was stable and could be extracted after aerobic storage at room temperature for 1 month, although at 105 degrees c the amount obtained was reduced. the dsrna was also recovered after leaves were freeze dried and stored in vacuo at room temperature for 6 months, or were dried and stored aerobically at room temperature for 10.5 months. dsrna of sugarcane fiji disease virus (fdv) was also s ... | 1991 | 1939508 |
| electrophoretic separation of dsrna genome segments from fiji disease and maize rough dwarf viruses. | by employing two different buffer solutions for polyacrylamide gel electrophoresis, all genome segments of fiji disease and maize rough dwarf virus were separated. fiji disease virus contains ten genome segments with approximate genome molecular weights of 19.26 x 10(6) and 19.85 x 10(6), depending on the buffer employed for electrophoresis. maize rough dwarf virus possesses ten dsrna segments and according to the buffer employed for electrophoresis the approximate molecular weights of the genom ... | 1975 | 18621350 |
| rna-dependent rna polymerase associated with subviral particles of fiji disease virus. | | 1976 | 4920 |
| morphology of fiji disease virus. | | 1977 | 841853 |
| detection and identification of fiji disease virus in infected sugarcane by immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assays. | radial double-immunodiffusion, immuno-osmophoretic and enzyme-linked immunosorbent assay (elisa) methods have been compared for the detection of fiji disease virus (fdv) in infected sugarcane tissue extracts using an antiserum containing antibodies specific to fdv proteins and ds-rna. elisa was the most sensitive of these tests and detected only fdv-specific proteins byt not ds-rna. immuno-osmophoretic tests were less sensitive than elisa but detected both the protein and ds-rna antigens as dist ... | 1981 | 6795221 |
| detection of fiji disease virus in infected sugarcane by nucleic acid hybridization. | clones of fiji disease virus were obtained by cdna synthesis from isolated viral genomic double-stranded rna. nick-translated probes made from a mixture of clones specifically detected fiji disease virus in infected sugarcane galls, leaves and growing tips. this method for detection of fiji disease virus is extremely sensitive, and can be used as a rapid screening procedure for the virus with very small tissue samples. | 1986 | 3755138 |
| chemiluminescent detection of fiji disease virus with biotinylated dna probes. | biotinylated fiji disease fijivirus specific cdna probes detected the presence of the virus in total nucleic acid extracts from infected sugarcane plants. hybridised biotinylated probes were detected with streptavidin-alkaline phosphatase conjugate and the light generating substrate amppd. samples were either blotted manually, or by alkaline capillary transfer using 100 mm naoh. transfer of nucleic acids to charge modified nylon with sodium hydroxide was superior to denaturation with glyoxal or ... | 1994 | 8031237 |
| faldaprevir (bi 201335) for the treatment of hepatitis c in patients co-infected with hiv. | chronic hcv infection affects 130-170 million individuals worldwide and there are currently 34 million people living with hiv/aids. the aim of treatment of hcv is the elimination of the virus (sustained virological response). with development of drugs that specifically target hcv replication, direct-acting agents, sustained virological response rates have dramatically changed for genotype 1 infections. challenges in the use of direct-acting agents in patients with hiv/hcv co-infection include th ... | 2014 | 24350778 |
| effect of d168v mutation in ns3/4a hcv protease on susceptibilities of faldaprevir and danoprevir. | hepatitis c virus (hcv) is a serious cause of liver inflammation, cirrhosis and the development of hepatocellular carcinoma. its ns3/4a serine protease functions to cleave a specific peptide bond, which is an important step in hcv replication. thus the ns3/4a protease has become one of the main drug-targets in the design and development of anti-hcv agents. unfortunately, high mutation rates in hcv have been reported due to the lack of rna proofreading activity resulting in drug resistance. herei ... | 2016 | 27731877 |
| Identification of yeast associated with the planthopper, Perkinsiella saccharicida: potential applications for Fiji leaf gall control. | Yeasts associate with numerous insects, and they can assist the metabolic processes within their hosts. Two distinct yeasts were identified by PCR within the planthopper Perkinsiella saccharicida, the vector of Fiji disease virus to sugarcane. The utility of both microbes for potential paratransgenic approaches to control Fiji leaf gall (FLG) was assessed. Phylogenetic analysis showed one of the microbes is related to yeast-like symbionts from the planthoppers: Laodelphax striatellus, Nilaparvat ... | 2011 | 21850475 |
| in vitro rearing of perkinsiella saccharicida and the use of leaf segments to assay fiji disease virus transmission. | fiji leaf gall (flg) is caused by the reovirus, fiji disease virus (fdv), which is transmitted to sugarcane by planthoppers of the genus perkinsiella. low vector transmission rates and slow disease symptom development make experimentation within the fdv-perkinsiella-sugarcane system inherently difficult. a laboratory-based technique was devised to rear the vector using sugarcane leaves as a food source. planthoppers were reared on sugarcane leaf segments embedded in agarose enclosed within plast ... | 2008 | 18943257 |
| purification and some properties of reovirus-like particles from leafhoppers and their possible involvement in wallaby ear disease of maize. | reovirus-like particles, occurring in association with viroplasms, crystalline arrays and tubules, in the cytoplasm of cicadulina bimaculata capable of inducing wallaby ear disease in maize, were purified from the insects by differential centrifugation, treatment with the nonionic detergent, nonidet p-40, and sucrose density gradient centrifugation. the purified particles have a double-shelled icosahedral structure about 70 nm in diameter with external projections (a spikes) about 10 nm long loc ... | 1980 | 18631635 |
| structure of the antigen from fiji disease virus particles eliciting antibodies specific to double-stranded polyribonucleotides. | antisera with fiji disease virus (fdv) protein-specific and double-stranded (ds) rna-specific antibodies were prepared by injecting rabbits with highly purified preparations of fdv cores. electron microscopic examination of negatively stained core preparations disclosed the presence of icosahedral particles, about 54 nm in diameter, with projections (b spikes) at their vertices as previously reported. however, observations on similar preparations by electron microscopy of specimens shadowed at a ... | 1979 | 18627900 |
| variation in acquisition of fiji disease virus by perkinsiella saccharicida (hemiptera: delphacidae). | fiji leaf gall, caused the fiji disease virus (genus fijivirus, family reoviridae, fdv), is a serious disease of sugarcane, saccharum officinarum l., in australia and several other asia-pacific countries. in australia fdv is transmitted only by the planthopper perkinsiella saccharicida kirkaldy (hemiptera: delphacidae), in a propagative manner. successful transmission of fdv by single planthoppers confined to individual virus free plants is highly variable, even under controlled conditions. the ... | 2008 | 18330111 |
| associations between dna markers and resistance to diseases in sugarcane and effects of population substructure. | association between markers and sugarcane diseases were investigated in a collection of 154 sugarcane clones, consisting of important ancestors or parents, and cultivars. 1,068 polymorphic aflp and 141 srr markers were scored across all clones. data on the four most important diseases in the australian sugarcane industry were obtained; these diseases being pachymetra root rot (pachymetra chaunorhiza b.j. croft & m.w. dick), leaf scald (xanthomonas albilineans dowson), fiji leaf gall (fiji diseas ... | 2006 | 17047910 |
| sequence analysis of genome segments s5 and s10 of mal de rio cuarto virus (fijivirus, reoviridae). | mal de rio cuarto virus (mrcv) was recently described as a new species of the genus fijivirus, family reoviridae. the nucleotide sequence of two mrcv genome segments was determined. mrcv s5 and s10 were predicted to encode proteins of 106.9 and 63.5 kda respectively. the protein coded by mrcv s5 had 62.8% and 35.7% identity to fijiviruses rbsdv s5 and fdv s5 coded proteins, and contained a rarely reported type-1 c-terminal peroxisomal targeting signal. the protein coded by mrcv s10 had identity ... | 2005 | 15747051 |
| purification and serology of viruslike particles from fiji disease virus-infected sugar cane. | | 1974 | 4213900 |
| occurrence of similar particles in fiji disease virus-infected sugar cane and insect vector cells. | | 1972 | 5017155 |
| genetic variability of genome segments 3 and 9 of fiji disease virus field isolates. | fiji leaf gall is an important disease of sugarcane in australia and other asia-pacific countries. the causative agent is the reovirus fiji disease virus (fdv). previous reports indicate that there is variation in pathology between virus isolates. to investigate the amount of genetic variation found in fdv, 25 field isolates from australia, papua new guinea and malaysia were analysed by partial sequencing of genome segments s3 and s9. there was up to 15% divergence in the nucleotide sequence amo ... | 2008 | 18299794 |