| the role of pityrosporum pachydermatis in otitis externa of dogs: evaluation of a treatment with miconazole. | the bacterial and mycotic flora were assessed in 158 ears of dogs with otitis externa and in 101 ears of healthy control dogs. pityrosporum pachydermatis occurred in 57 per cent of ears with otitis externa and in 17 per cent of clinically healthy ears. staphylococci and pseudomonas aeruginosa were the predominant bacteria in otitic ears, micrococci and bacillus spp were the most frequent isolates from clinically healthy ears. p pachydermatis, ps aeruginosa and candida tropicalis occurred in mono ... | 1979 | 109986 |
| pityrosporum pachydermatis "canis" as a major cause of otitis externa in dogs. | | 1979 | 460302 |
| malassezia pityrosporum pachydermatis (weidman) dodge 1935. | priority of the name malassezia pachydermatis (weidman) dodge 1935 is indicated for the microorganism which has been called pityrosporum pachydermatis weidman 1925 and p. canis gustafson 1955. m. pachydermatis is here further characterized in culture with information drawn from 2 recent isolates, in particular the presence of spiral grooves on the inner surface of the cell wall, good growth on mycosel agar, rapid production of urease, and assimilation of glucose by the wickerham method. | 1979 | 531721 |
| pityrosporum pachydermatis in a black bear (ursus americanus). | pityrosporum pachydermatis was repeatedly isolated from portions of alopecic tissue from the throax and ears of a black bear cub (ursus americanus). yeastlike cells morphologically identical with those of p. pachydermatis were observed in stained tissue sections. this is the first reported association of this yeast with a member of the family ursidae. | 1978 | 635724 |
| the association of pityrosporum pachydermatis with the normal external ear canal of dogs and cats. | | 1976 | 933471 |
| letter: pityrosporum pachydermatis in pendulous and erect ears of dogs. | | 1976 | 1064816 |
| antigen structure of pityrosporum pachydermatis. | the purpose of the present work was to define the antigen structure of pityrosporum pachydermatis. the prepared antigens and their respective antisera were studied by means of double diffusion test in an agar gel and crossed immunoelectrophoresis. the study made it possible to prove marked quantitative differences between some antigen fractions of a few strains of p. pachydermatis. it has not been reported, however, that there occur either of specific antigens or reaction of partial serological ... | 1992 | 1339570 |
| otitis externa induced with malassezia pachydermatis in dogs and the efficacy of pimaricin. | eight beagles were experimentally inoculated intraotally with malassezia pachydermatis to induce acute otitis externa. three or 4 days after the inoculation, the animals showed the symptoms of otitis externa. all ear canals were erythematous and the dogs were shaking their heads. a large number of m. pachydermatis was noticed in exudate taken from every ear canal. clinical signs of otitis externa were reduced after treatment with 0.1 ml (per canal) of 1% pimaricin suspension twice a day for 3 da ... | 1992 | 1391168 |
| factors associated with and prevalence of high malassezia pachydermatis numbers on dog skin. | the prevalence of cutaneous malassezia spp was evaluated in a semiquantitative fashion at 3 sites on 98 dogs examined because of various dermatoses. thirty (10.2%) of the sites and 19 (19.4%) of the dogs had malassezia spp amounts higher than that found on grossly normal skin. the prevalence of higher than normal amounts did not correlate significantly with sample site, sex, or age. the factors associated with an increased prevalence of increased malassezia spp counts were seborrheic dermatitis, ... | 1992 | 1399796 |
| [bacteriology and mycology of otitis externa in dogs]. | the bacterial and fungal flora of 1118 ears of dogs with otitis externa and 100 ears of healthy control dogs were studied in order to isolate the causative agents. the yeast malassezia pachydermatis (56%) was by far the most common organism in otitic dogs followed by the bacteria staphylococcus intermedius (23%), pseudomonas aeruginosa (12%), proteus spp. (6%) and streptococcus canis (5%). a statistical analysis of observed results showed that the incidence of these organisms is significant in o ... | 1992 | 1411421 |
| chromosome-sized dna of malassezia pachydermatis by pulsed-field gel electrophoresis. | the genome of malassezia pachydermatis isolates from dogs was resolved into six chromosomes by using pulsed-field gel electrophoresis and their molecular sizes were calculated as 820, 1,100, 1,400, 1,470, 1,660 and 1,820 kb, respectively. comparison of electrophoretic patterns suggested that the chromosomes of m. pachydermatis were homozygous. | 1992 | 1477176 |
| human infections due to malassezia spp. | the genus malassezia contains three member species: malassezia furfur and malassezia sympodialis, both obligatory lipophilic, skin flora yeasts of humans, and malassezia pachydermatis, a nonobligatory lipophilic, skin flora yeast of other warm-blooded animals. several characteristics suggest the basidiomycetous nature of these yeasts, although a perfect stage has not been identified. classically, these organisms are associated with superficial infections of the skin and associated structures, in ... | 1992 | 1576583 |
| malassezia pachydermatis: properties and storage. | five strains of malassezia pachydermatis isolated from small animals in bratislava were subcultured, maintained under sterile paraffin oil and preserved in liquid nitrogen. morphological and physiological characteristics as well as the analysis of long chain fatty acids of the cell mass were determined. the results showed that the maintenance in liquid nitrogen is the most satisfactory method of preservation of pure cultures of malassezia pachydermatis. | 1991 | 1803241 |
| ultrastructure of budding process of malassezia pachydermatis. | the ultrastructure of malassezia pachydermatis and its budding process was investigated by scanning and transmission electron microscopy. the innermost layer of the cell wall showed the serrated structure characteristic of the genus malassezia. in the daughter cell, this structure became more defined as the cell grew. the mode of conidium ontogeny was monopolar blastic development with a collarette. the appearance was similar to that reported previously for malassezia furfur, with the following ... | 1991 | 1815031 |
| [in vitro antifungal activity of amorolfine against malassezia species]. | in vitro antifungal activities of a new morpholine agent, amorolfine (mt-861) were investigated, against 39 strains of malassezia furfur (11 stock cultures and 28 clinical isolates) and 8 strains (stock cultures) of malassezia pachydermatis, in comparison with those of 2 reference drugs, clotrimazole (ctz) and bifonazole (bfz). of the 3 antifungal agents, mt-861 exhibited strongest antifungal activities against the stock cultures of m. furfur and m. pachydermatis with average mic values of 0.428 ... | 1991 | 1960851 |
| the in vitro antifungal activity of ketoconazole, zinc pyrithione, and selenium sulfide against pityrosporum and their efficacy as a shampoo in the treatment of experimental pityrosporosis in guinea pigs. | the fungistatic and fungicidal activity of ketoconazole, zinc pyrithione, and selenium sulfide against pityrosporum, a yeast thought to play a pathogenic role in seborrheic dermatitis and dandruff, was assessed in dixon broth for pityrosporum ovale and sabouraud broth for pityrosporum pachydermatis. ketoconazole inhibited growth at concentrations ranging from 0.001 to 1 micrograms/ml. for zinc pyrithione and selenium sulfide higher concentrations were needed. in a guinea pig model the efficacy o ... | 1990 | 2142498 |
| malassezia pachydermatis isolation from a scarlet macaw. | a repeated isolation of malassezia pachydermatis weidman from a scarlet macaw is reported. this is the first report of birds infected with this yeast. | 1990 | 2267001 |
| in vitro activity of five antifungal agents against malassezia pachydermatis. | | 1990 | 2391785 |
| [pityrosporum fungi in the human--a review and a case report]. | pityrosporum pachydermatis could be isolated out of a smear taken from the auditory meatus. the occurrence of this zoophilic species prompted us to discuss the systematic position and nomenclature of pityrosporum yeasts in man. | 1989 | 2686241 |
| a reevaluation of the genus malassezia by means of genome comparison. | results of a study of the genus malassezia on the basis of genome characters confirm that two species should be maintained, m. furfur and m. pachydermatis. the two forms associated with skin disease, frequently referred to as pityrosporum orbiculare and p. ovale, were found to be synonymous, the name m. furfur having priority. malassezia pachydermatis, hitherto regarded as a strictly zoophilic species, may also be found on humans. | 1989 | 2757367 |
| evaluation of cilofungin, a lipopeptide antifungal agent, in vitro against fungi isolated from clinical specimens. | cilofungin (ly121019) is a new lipopeptide antifungal drug. we tested this drug against 141 pathogenic fungal isolates. all fungal species were tested by broth dilution at 35 degrees c. malassezia furfur was tested by agar dilution. the results demonstrate the specificity of cilofungin activity. candida albicans, candida tropicalis, and malassezia pachydermatis were highly susceptible, whereas candida parapsilosis, candida pseudotropicalis, candida krusei, torulopsis glabrata, blastomyces dermat ... | 1989 | 2802565 |
| recovery of malassezia pachydermatis from eight infants in a neonatal intensive care nursery: clinical and laboratory features. | a 15-month retrospective survey of 507 admissions to a neonatal intensive care unit revealed 8 patients from whom malassezia pachydermatis was isolated from one or more clinical specimens. the fungus was cultured from blood (four patients), central venous catheter tips (three patients), urine (four patients), cerebrospinal fluid (one patient), eye discharge (one patient), ear discharge (one patient) and tracheal aspirate (one patient). seven of the eight infants displayed an episode of one or mo ... | 1988 | 3134645 |
| preliminary studies on the mechanism of infection and characterization of malassezia pachydermatis in association with canine otitis externa. | a study on the mechanism of infection and the characterization of malassezia pachydermatis in connection with canine otitis externa was conducted. the ability of this yeast to grow uninhibited in intimate contact with the diversity of other microbial isolants of the canine aural canal was demonstrated. canine cerumen seemed to promote the growth of this yeast. although the source of infection is still obscure, m. pachydermatis managed to survive in soil and dust at different temperatures for fou ... | 1988 | 3146702 |
| clinical and microbiological features of infection with malassezia pachydermatis in high-risk infants. | malassezia pachydermatis, a yeast that has not previously been implicated as a cause of human disease, was isolated from cultures of blood from three infants. all infants were 25-27 w of gestational age and had multiple underlying medical problems. the infants had been hospitalized for at least six weeks, had received broad-spectrum antibiotics, and had received parenteral lipid nutrition via a central venous catheter. in one patient, fungemia was accompanied by clinical and laboratory evidence ... | 1988 | 3373021 |
| malassezia pachydermatis in small animals. | | 1987 | 3444444 |
| association of malassezia pachydermatis with systemic infections of humans. | thirty-two malassezia spp. isolates from human clinical specimens represented m. furfur and m. pachydermatis. both species reportedly were obtained from patients with similar febrile systemic syndromes, including infections of the lungs or other tissues. | 1987 | 3654952 |
| studies on the isolation, growth and maintenance of malassezia pachydermatis. | results related to the isolation, cultivation, culture and maintenance of the opportunistic pathogen malassezia pachydermatis are reported. a dextrose nutrient medium with 1.5% yeast extract turned out to be the most favourable medium for its development. it permitted identification in 24 hours and maintenance of isolates for three months without subculturing. addition of tween 80 (1%) significantly enhanced the isolation of this yeast from clinical materials. | 1987 | 3657903 |
| in vitro sensitivity of malassezia spp. to various antimycotics. | the sensitivity of malassezia furfur and malassezia pachydermatis to various antimicrobial agents both as single compounds and combined with various vehicles was evaluated in vitro using the agar diffusion method. clotrimazole, thiabendazole, ketoconazole, econazole, miconazole and other agents were chosen taking into account their specific commercial formulations and their utilization in mycotic pathologies sustained by malassezia genus in man and animals. the antimicrobial agents were compared ... | 1985 | 3836136 |
| [comparison of various media and various methods for the growth and isolation of pityrosporum pachydermatis]. | the pathogenic role of pityrosporum pachydermatis in otitis externa of dogs and the related diagnostic problems are emphasized. we report results related to isolation, cultivation and identification of yeast. agar nutritive glucosate with 1,5% of yeast extract has been showed as the best medium permitting identification in 24 hrs, associated with morfological test. tween 80 integration (1%) to the medium permits to isolate lipolitic yeasts also. | 1984 | 6370273 |
| a survey of 120 isolates of malassezia (pityrosporum) pachydermatis. preliminary study. | the morphological, cultural and biochemical characteristics of 120 isolates of malassezia (pityrosporum) pachydermatis, isolated from chronic otitis externa in the dog, are discussed. | 1984 | 6727981 |
| [proteolysis by pityrosporum pachydermatis]. | | 1982 | 6757747 |
| serologic analysis of the extractable carbohydrate antigens of pityrosporum ovale. | antigens soluble in hot trichloracetic acid from eighteen strains of pityrosporum ovale were studied using rabbit antiserum to american type culture collection strains 24027, 12078, and 14521. these strains were found to share two antigens, both of which were present in cells of pityrosporum orbiculare and one of which was present in cells of pityrosporum pachydermatis, pityrosporum canus, candida albicans and rhodotorula rubrum. | 1980 | 6779094 |
| association of malassezia (pityrosporum) pachydermatis with sarcoptic mange in new york state. | malassezia (pityrosporum) pachydermatis consistently accompanied sarcoptic mange in all red foxes (vulpes fulva), porcupines (erethizon dorsatum), and coyotes (canis latrans) examined. this yeastlike microorganism has not heretofore been reported on any of these hosts. its presence on the exoskeleton of sarcoptes scabiei taken from these animals suggests a carrier role for the mite. the yeast may be saprophytic or a secondary pathogen. | 1980 | 6780699 |
| population sizes and frequency of malassezia pachydermatis at skin and mucosal sites on healthy dogs. | skin and mucosal carriage of malassezia pachydermatis was studied in 20 healthy pet dogs of various breeds and in 20 kennelled beagles. using swabs, anal carriage was detected in 10 pet dogs and 11 beagles and the nose, mouth, prepuce and vulva were shown to be infrequently colonised. m pachydermatis was isolated from the external ear canal of 11 beagles and two pet dogs; both the population sizes and frequency of isolation were significantly (p < 0.05) greater in the beagles. the yeast was infr ... | 1995 | 7603055 |
| characterization of markedly lipid-dependent malassezia pachydermatis isolates from healthy dogs. | when 244 malassezia colonies which had been isolated from a colony of beagle dogs using modified dixon's agar were sub-cultured on sabouraud's dextrose agar to determine their lipid dependence, 30 showed poor growth resembling m. furfur, whereas the remainder were typical of m. pachydermatis. eight of the 10 poor growing isolates selected for further study formed colonies typical of m. pachydermatis after five passages on sabouraud's dextrose agar at 4 d intervals and two continued to show poor ... | 1995 | 7759383 |
| evaluation of a detergent scrub technique for the quantitative culture of malassezia pachydermatis from canine skin. | a detergent scrub technique using wash fluid consisting of 0.075 m phosphate-buffered saline, ph 7.9 containing 0.1 per cent triton x-100 was evaluated for the quantitative culture of malassezia pachydermatis from canine skin. preliminary studies showed that the detergents triton x-100, tween 40 and tween 80 were equally able to disperse suspensions of pure cultures of m pachydermatis, but that the yeast counts were significantly reduced (p < 0.001) after suspension in saline, triton x-100 or tw ... | 1995 | 7761691 |
| confirmation of the nomenclatural status of malassezia pachydermatis. | malassezia strains from dogs and rhinoceros all proved identical using mole% g+c and ndna/dna reassociation experiments. the use of the name malassezia pachydermatis, originally described for a strain isolated from a rhinoceros, is thus justified for non lipid-dependent strains of other sources. | 1995 | 7771764 |
| comparison of two shampoos for treatment of malassezia pachydermatis-associated seborrhoeic dermatitis in basset hounds. | a randomised-double-blind parallel study compared the clinical and antimicrobial efficacies of a miconazole-chlorhexidine shampoo with a selenium sulphide shampoo for the treatment of seborrhoeic dermatitis associated with malassezia pachydermatis in 33 basset hounds. all 16 miconazole-chlorhexidine treated hounds and 11 of 17 selenium sulphide treated hounds improved when shampooed at three-day intervals for three weeks. the miconazole-chlorhexidine treated hounds showed significantly greater r ... | 1995 | 7783442 |
| monitoring spread of malassezia infections in a neonatal intensive care unit by pcr-mediated genetic typing. | malassezia furfur and malassezia pachydermatis were isolated from newborn children and incubators in a neonatal intensive care unit. to assess whether persistence or frequent import of the organisms was the cause of the elevated incidence, genetic typing of the strains was performed by pcr-mediated dna fingerprinting. by using pcr primers aimed at repeat consensus motifs, six different genotypes could be detected in a collection of six m. furfur reference strains. in the case of 10 m. pachyderma ... | 1994 | 7814492 |
| pathogenic aerobic bacteria and fungi isolated from stray dogs in trinidad. | the occurrence of pathogenic aerobic bacteria and fungi in 100 stray dogs was investigated. the most commonly isolated pathogens were staphylococcus aureus, s. intermedius and malassezia pachydermatis. no zoonotic pathogen was isolated. it is suggested that stray dogs may not maintain or transmit the pathogens. | 1994 | 7863067 |
| the fungal flora of zoo animals' ears. | the mycotic flora of the ears of zoo animals was investigated in a large zoological garden in germany. malassezia pachydermatis was isolated from the following animals: big ant-eater, brown bear, common wombat, eurasian badger, indian elephant, mangaliza pig and wide-mouthed rhinoceros. aspergillus and penicillium species, yeasts and zygomycetes were also isolated from some animals. | 1994 | 7935595 |
| sterol composition in polyene antibiotic-sensitive and resistant strains of malassezia pachydermatis. | the membrane sterol compositions of ten strains of malassezia pachydermatis were analysed by gas-liquid chromatography. six of these were wild-type strains from clinical sources that were sensitive to polyene antibiotics, whereas the other four strains were resistant mutants that had arisen after treatment with n-methyl-n'-nitrosoguanidine or uv radiation. the wild-type strains contained 838.8 to 1291.7 micrograms of sterol per gram of freeze-dried mycelium. the sterols were composed of 79% ergo ... | 1994 | 7985380 |
| [microbiological results of ear swabs from dogs and cats]. | 413 swabs of dogs and cats ears were examined microbiologically. bacteria could be isolated in 82% and yeasts or moulds in 28% of the cases. the most common bacteria species were coagulase-positive staphylococci, pseudomonas spp., beta-hemolytic streptococci, and proteus spp. with staphylococci being the most common isolate. within the group of yeasts and moulds malassezia pachydermatis was isolated most frequently (90%). from the results of in vitro antibiotic tests, a general recommendation fo ... | 1994 | 8165666 |
| nosocomial malassezia pachydermatis bloodstream infections in a neonatal intensive care unit. | malassezia pachydermatis, a lipophilic yeast, has been described to cause sporadic nosocomial bloodstream infections (bsi). nosocomial outbreaks of m. pachydermatis bsi have never been described. a cluster of m. pachydermatis bsis in the neonatal intensive care unit at louisiana state university medical center, university hospital provided the opportunity to investigate the epidemiology of this organism and apply molecular epidemiologic typing techniques. a case-patient was defined as any neonat ... | 1994 | 8190533 |
| the use of karyotyping in the systematics of yeasts. | the use of electrophoretic karyotyping in systematics of yeasts is discussed. new data are provided on the karyotypes of the medically important fungi hortaea werneckii, filobasidiella (= cryptococcus) neoformans, and malassezia species. hortaea werneckii has twelve to eighteen bands of chromosomal dna, ranging in size between 500 and 2300 kb. the karyotypes of filobasidiella neoformans consist of seven to fourteen bands of chromosomal dna. the varieties neoformans and bacillispora cannot be sep ... | 1993 | 8259832 |
| the distribution of malassezia furfur and malassezia pachydermatis on normal human skin. | clinically normal skin of 200 volunteers, age range 20-30 years, were studied. skin samples were taken by swabbing ten different sites and examined for the presence of malassezia furfur and malessezia pachydermatis by culture. all of the subjects studied harbored m. furfur at every site tested, and had high counts on the head region and upper trunk. the strains isolated could be divided on the basis of culture characteristics into three distinct groups. only 12% of the subjects carried m. pachyd ... | 1993 | 8266240 |
| effects of fatty acids on the growth and composition of malassezia pachydermatis and their relevance to canine otitis externa. | malassezia pachydermatis is one of the many infective agents involved in canine otitis externa. two major phenotypes (large and small colony types), which had different fatty acid compositions, were found among strains of m pachydermatis isolated from canine ears or skin. the large-colony type contained palmitic, stearic, oleic and linoleic acids, whereas the small-colony type contained myristic, palmitic, stearic, linoleic acids and five other unidentified fatty acids. exogenous saturated fatty ... | 1993 | 8378605 |
| production and use of monoclonal antibodies to microsporum canis. | microsporum canis ncpf 179 and m. canis ncpf 177 dermatophyte cytoplasmic extracts (dces) were used as antigens to generate monoclonal antibodies (mabs). hybridomas with supernatants of optical density (od) > 1 for homologous dermatophyte cytoplasmic extracts (ce) and od < 0.5 for heterologous ce of candida albicans tested by the enzyme-linked immunosorbent assays (elisa) were selected for cloning. mabs secreted by cloned hybridoma lines were screened against ce of m. canis, m. gypseum, m. equin ... | 1995 | 8560740 |
| quantity and distribution of malassezia organisms on the skin of clinically normal dogs. | to define the extent to which malassezia organisms can be recovered from the skin of clinically normal dogs and to assess differences in organism recovery related to anatomic sampling site and to method of collection. | 1996 | 8621316 |
| factors associated with elevated cutaneous malassezia pachydermatis populations in dogs with pruritic skin disease. | the prevalences of breeds and concurrent diseases in a group of 40 dogs with pruritic skin disease associated with elevated cutaneous malassezia pachydermatis populations were compared with samples of a dermatological hospital population. the ages and genders of the affected dogs were comparable to those of the dermatology population. basset hounds, cocker spaniels and west highland white terriers were significantly overrepresented. concurrent diseases were diagnosed in 27 dogs, of which 15 were ... | 1996 | 8683952 |
| isolation of malassezia sympodialis from feline skin. | carriage of malassezia yeasts was investigated in 17 cats in two colonies using a lipid-supplemented culture medium. malassezia pachydermatis was isolated from one cat. lipid-dependent malassezia yeasts with electrophoretic karyotypes consistent with m. sympodialis were isolated from all six cats in one group and from one of 11 in the second group. to our knowledge, this is the first report of the isolation of lipid-dependent yeasts from cats. | 1996 | 8732361 |
| malassezia pachydermatis with special reference to canine skin disease. | a review of the diseases caused by malassezia pachydermatis has led to the conclusion that the yeast is an opportunistic pathogen that depends on predisposing host factors and different immune suppressive mechanisms for clinical manifestation. until recently, the role of m. pachydermatis in seborrhoeic dermatitis and otitis externa in dogs has been largely unrecognized. the clinical manifestation, aetiology, diagnosis and treatment of disease conditions in dogs caused by m. pachydermatis are rev ... | 1996 | 8762604 |
| comparison of different methods of maintenance of malassezia pachydermatis (s. pityrosporum pachydermatis) strains. | the maintenance of malassezia pachydermatis isolated from canine otitis and dermatitis was compared on sabouraud dextrose agar slants under liquid paraffin, an broth and in water, and by paper replica method, silica gel method, freeze-drying and deep freezing at-80 degrees c. hydrolase production before and after storage was determined with api-zym bio-merieux kits. deep freezing and freeze-drying were the best methods of maintaining m. pachydermatis. hydrolase production was stable throughout t ... | 1996 | 8795259 |
| evaluation of selected physiological and morphological characteristics of pityrosporum pachydermatis isolated from clinical cases of otitis externa and dermatitis in dogs and cats. | physiological properties and proportion of typical features of pityrosporum pachydermatis were determined on 385 strains from clinical cases of o.e and dermatitis in dogs. carbohydrates and nitrogen assimilation were determined auxanographically. urease production and enzyme release were assessed on christensen's medium and api-zym respectively. all strains oxidised carbohydrates in the of test. 90% assimilation of glucose and production of urease are typical of pityrosporum, contrary to 100% po ... | 1994 | 8891171 |
| comparison of media and conditions of incubation for the quantitative culture of malassezia pachydermatis from canine skin. | fifty-five swab-wash specimens from dogs were cultured at 32 degrees c on sabouraud's dextrose agar either with or without 1 per cent tween 80. ushijima's medium a and modified dixon's and leeming's media. the counts of malassezia pachydermatis were not significantly different after three or seven days of incubation. colony counts on contact plates were significantly greater after incubation for seven days on sabouraud's dextrose (p < 0.001) and modified dixon's agars (p < 0.05) than after three ... | 1996 | 8938862 |
| characteristics of malassezia pachydermatis strains isolated from canine otitis externa. | the morphological, cultural and biochemical characteristics of 80 m. pachydermatis strains isolated from cases of canine otitis externa were studied. microscopically, the strains could be subdivided into two phenotypes. all m. pachydermatis strains grew well on sabouraud glucose, yeast morphology and modified malt extract agar, but formed two distinct colony types. all strains were characterized by no fermentation. assimilation of glucose, mannitol (42 strains), sorbitol (40 strains) and peptone ... | 1996 | 9009653 |
| new combination for the therapy of canine otitis externa. i. microbiology of otitis externa. | in order to compound a new drug combination against canine otitis externa (oe), 515 dogs affected with oe were subjected to physical examination and microbiological analysis of their ear exudates. oe was erythematous-ceruminous in 83 per cent and suppurative in 17 per cent of the patient material. erythematous-ceruminous inflammations were characterised by severe pruritus and accumulation of brownish, greasy cerumen in the auditory canal. the yeast malassezia pachydermatis was isolated from the ... | 1997 | 9065882 |
| an immunohistochemical study of the lesions of demodicosis in the dog. | histopathological and immunohistochemical examination of skin biopsies from 32 dogs with demodicosis is reported. there was no association between the different clinical presentations of the disease and the histopathological character of the biopsies, which included absence of inflammation (n = 2), dominant perifolliculitis (n = 11), interface mural folliculitis (n = 7), mural folliculitis (n = 1), furunculosis (n = 10) and nodular dermatitis (n = 1). in eight of 32 biopsies colonies of coccoid ... | 1997 | 9131435 |
| epidemiological analysis of malassezia pachydermatis isolates by partial sequencing of the large subunit ribosomal rna. | the opportunistic yeast malassezia pachydermatis is commonly recovered from both normal and diseased skin of warm-blooded animals. the diversity of m pachydermatis isolates obtained from a wide range of hosts was investigated by the partial sequencing of the large subunit (lsu) ribosomal rna. among 100 isolates examined, seven types (ia-ig) were discriminated on the basis of nucleotide sequence diversity. the seven types differed by one to five mutations, all of them corresponding to transitions ... | 1997 | 9160419 |
| in vitro activity of climbazole, clotrimazole and silver-sulphadiazine against isolates of malassezia pachydermatis. | malassezia pachydermatis is a yeast-like, mainly zoophilic fungus, also known as malassezia canis. it can be isolated in 20-50% from normal ear cerumen specimen from dogs or cats and has an even higher prevalence in non-suppurative otitis externa in animal species such as these. the genus malassezia contains the two species m. furfur, a mainly anthropophilic fungus which physiologically occurs on human skin, and m. pachydermatis. we performed minimal inhibitory concentration (mic)-testings of 40 ... | 1997 | 9230670 |
| [results of bacteriologic and mycologic investigations of otitis media in dogs]. | this study describes the bacteriological and mycological investigation of 212 ear swab samples of dogs with an otitis externa. 11 samples were sterile. malassezia pachydermatis could be cultivated in 72.1% of all samples, in 21.9% of these cases in pure culture. in 27.9% of all samples only bacteria were found. coagulase-positive haemolytic staphylococcus and malassezia pachydermatis were isolated together in 31.8% of all ear swab samples. pseudomonas aeruginosa could be detected in 22.9%, haemo ... | 1997 | 9289887 |
| resolution of exfoliative dermatitis and malassezia pachydermatis overgrowth in a cat after surgical thymoma resection. | a four-year-old, male neutered domestic shorthaired cat was presented with a two-week history of nasal and ocular discharge, generalised exfoliative dermatitis, intense pruritus, polydipsia, polyphagia, weight loss, intermittent hindlimb ataxia and lethargy. cutaneous populations of malassezia pachydermatis yeast organisms were found to be elevated. the generalised nature of the disease prompted survey radiography which revealed the presence of a cranial mediastinal mass which was subsequently r ... | 1997 | 9358405 |
| an epidemic of malassezia pachydermatis in an intensive care nursery associated with colonization of health care workers' pet dogs. | malassezia species are lipophilic yeasts that are emerging as nosocomial pathogens, particularly in low-birth-weight neonates who receive lipid emulsions. when a cluster of patients with malassezia pachydermatis infection was identified in an intensive care nursery, we initiated an investigation. | 1998 | 9494146 |
| dermatitis caused by malassezia pachydermatis in a california sea lion (zalophus californianus). | | 1998 | 9569500 |
| protein profiles of malassezia pachydermatis isolated from dogs. | the protein fractions of 30 malassezia pachydermatis isolates from dogs (15 with otitis and 15 with dermatitis) were investigated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page). the electrophoresis runs resulted in bands ranging from 15.0-145.7 kda in the ear canal secretion isolates. the values for the skin isolates varied between 16.0-141.1 kda. the presence of protein bands occurred most frequently within the 30-40 kda bands (66.7%), 40-50 kda (70.0%), 50-60 kda (73. ... | 1997 | 9608717 |
| type-1 hypersensitivity reactions to malassezia pachydermatis extracts in atopic dogs. | to investigate the potential allergenic role of the yeast malassezia pachydermatis in dogs with clinical diagnosis of atopic dermatitis. | 1998 | 9659547 |
| effect of topical therapy of malassezia pachydermatis-associated seborrhoeic dermatitis on oral carriage of m pachydermatis. | | 1998 | 9682432 |
| malassezia pachydermatis infections. | | 1998 | 9687254 |
| malassezia pachydermatis infections. | | 1998 | 9687255 |
| humoral and cell-mediated responses to malassezia pachydermatis in healthy dogs and dogs with malassezia dermatitis. | the in vitro proliferative responses of peripheral blood mononuclear cells from seven healthy basset hounds exposed to malassezia pachydermatis antigen (500 micrograms/ml) exceeded (p < 0.05) those of seborrhoeic basset hounds with high populations of m pachydermatis and eight irish setters with gluten-sensitive enteropathy. the stimulation indices in the latter two groups and in eight healthy beagles were comparable. the stimulation indices of the four groups after exposure to phytohaemaglutini ... | 1998 | 9802194 |
| emerging infectious diseases and pathogens. | emerging infectious diseases are caused by old, new, and mutant microorganisms. emergence of these pathogens can be attributed to changes in the characteristics and risk factors of patients, the widespread use of antibiotics, changes in the environment, the role of xenotransplantation, and international travel. in the united states, the incidences of c. difficile, cyclosporiasis, enterohemorrhagic e. coli gastroenteritis, hantavirus, hepatitis c virus infection, and lyme disease have increased s ... | 1999 | 10318733 |
| activity of chlorhexidine shampoos in vitro against staphylococcus intermedius, pseudomonas aeruginosa and malassezia pachydermatis. | | 1999 | 10378283 |
| genetic diversity in the yeast species malassezia pachydermatis analysed by multilocus enzyme electrophoresis. | fifty-two strains of the yeast species malassezia pachydermatis were analysed by multilocus enzyme electrophoresis. m. pachydermatis appeared to be genetically heterogeneous. a total of 27 electrophoretic types were identified that could be divided into five distinct groups with different host specificities. the diversity revealed by this electrophoretic method matched remarkably well the reported genetic variability obtained by comparing large subunit rrna sequences. this study also suggests th ... | 1999 | 10425792 |
| recovery of microsporum gypseum and malassezia pachydermatis from the nasal bridge in various dog groups. | | 1999 | 10466776 |
| prevalence of malassezia spp. in the ears of asymptomatic cattle and cattle with otitis in brazil. | yeasts of the genus malassezia are lipophilic microorganisms that are saprophytes that can act as opportunistic pathogens in animals. malassezia pachydermatis is commonly isolated from the ear canal and skin of healthy dogs, or in association with seborrheic dermatitis and otitis externa conditions. the objective of the present study was to determine the occurrence of malassezia spp. in the ears of healthy bovines and bovines with otitis. specimens (secretion or cerumen) were collected with ster ... | 1999 | 10498444 |
| malassezia pachydermatis: a review. | malassezia pachydermatis is of importance in both veterinary and human medicine. its taxonomic status and physiological characteristics are now better understood. skin disease associated with this lipophilic yeast is now commonly recognized, especially in dogs. however, further studies are required to elucidate the mechanisms which allow this yeast to proliferate and induce disease. skin colonization is common in pet carnivores which consequently constitute a source of m. pachydermatis for susce ... | 1999 | 10520154 |
| characterization of malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (pfge). | a total of 220 isolates of the genus malassezia obtained from affected skin of animals and humans were included in this study. the isolates were examined for their belonging to the known species malassezia pachydermatis, malassezia sympodialis, malassezia furfur, malassezia obtusa, malassezia restricta, malassezia globosa and malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (tween 20, 40, 60, 80). t ... | 1999 | 10536433 |
| molecular heterogeneity in clinical isolates of malassezia pachydermatis from dogs. | molecular investigation of 16 strains, conventionally identified to be malassezia pachydermatis, isolated from dogs in japan was carried out by random amplification of polymorphic dna (rapd) and chitin synthase 2 (chs2) gene sequence analyses. the rapd band patterns of 13 clinical isolates were identical to that of standard strain of m. pachydermatis (cbs-1879). the other three clinical isolates were different from the standard strain of m. pachydermatis in rapd patterns, and two of the three is ... | 1999 | 10591498 |
| detection by elisa of the humoral immune response in rabbits naturally infected with trichophyton mentagrophytes. | an indirect enzyme linked immunosorbent assay (elisa) was developed and its diagnostic potential evaluated for rabbits infected by trichophyton mentagrophytes. within-run and between-run coefficient of variance varied from 2.3 to 7.7% and from 5.9 to 8.5%, respectively, indicating satisfactory reproducibility of the elisa. there was no significant cross-reaction with antigens of microsporum canis, malassezia pachydermatis and aspergillus fumigatus. the level of specific igg to trichophyton menta ... | 1999 | 10591499 |
| clinical features and treatment of malassezia folliculitis with fluconazole in orthotopic heart transplant recipients. | orthotopic heart transplant recipients need immunosuppressive treatment and are at an increased risk for opportunistic infections such as malassezia folliculitis. during a 4-month period (july to october 1990), 11 such cases were identified and treated; all were male with mean age of 43+/-9 years and on standard triple immunosuppressive therapy. skin scrapings in potassium hydroxide (koh) preparation with microscopy and/or culture identified either malassezia furfur or malassezia pachydermatis a ... | 2000 | 10703699 |
| proteinase, phospholipase, hyaluronidase and chondroitin-sulphatase production by malassezia pachydermatis. | the production of four functional enzyme categories was investigated in 30 strains of malassezia pachydermatis isolated from dogs with otitis or dermatitis. the most appropriate reading intervals for these assays were determined with the aid of statistical comparisons. all strains produced proteinase and chondroitin-sulphatase; hyaluronidase and phospholipase were produced by all skin isolates (15/15) and 14 out of 15 ear canal isolates. strains from ear canals did not differ significantly as a ... | 2000 | 10746230 |
| colonisation status of malassezia pachydermatis on the hair and in the hair follicle of healthy beagle dogs. | hair and hair follicle carriage of malassezia pachydermatis was studied in 12 healthy beagle dogs. the yeast was isolated from hair clipped from the lip region at 13 sites in nine dogs but was less frequently recovered from the interdigital spaces on the forefeet and from two sites on the trunk. population sizes at the lip were significantly greater (p < 0.01) than those at other sites. skin biopsy specimens were obtained from the same sites and epidermal and follicular tissues dissected followi ... | 2000 | 10877978 |
| malassezia pachydermatis isolated from a south american sea lion (otaria byronia) with dermatitis. | a fungus was isolated from the skin of an otaria byronia and from the water of the pool in which the animal was kept. it formed creamy colonies with soft texture on dixon agar and grew well without supplements of long-chain fatty acids. cells were ovoid to cylindrical in shape, budded from a broad base, and budded and divided at the same location. thus, the isolate was identified as m. pachydermatis. we compared this very rare isolate from a marine mammal with four strains of m. pachydermatis us ... | 2000 | 10993190 |
| optimum incubation conditions for the isolation of yeasts from canine otitis externa. | samples from otitis externa in the dog were cultured on a variety of media under different conditions of incubation in order to assess the most suitable conditions, especially for the isolation of malassezia pachydermatis. columbia blood and macconkey agar at 37 degrees c for 24 h with air and sabouraud glucose agar under microaerophilic conditions for 48 h at 37 degrees c were considered the ideal combination. | 2000 | 11075548 |
| adherence of malassezia pachydermatis and malassezia sympodialis to canine, feline and human corneocytes in vitro. | | 2000 | 11079443 |
| study of lipid in the ear canal in canine otitis externa with malassezia pachydermatis. | an epidemiological investigation of 120 canine otitis externa cases in 1,370 dogs was done on the incidence rate, ear pinna shapes, breeds and their relationships. eighty-five cases (12.6%) in 672 dogs with pendulous ears and 35 cases (5.0%) in 698 dogs with erect ears had otitis externa, and the difference between them was significant (p<0.05). ninety-five auditory cerumen specimens were cultured for malassezia pachydermatis (m. pachydermatis) and analyzed for concentrations of major fatty acid ... | 2000 | 11129861 |
| the influence of newly synthesised fenporpimorph derivatives on some pathogen yeasts. | the effect of minimum inhibitory concentrations (mics) of six novel fenpropimorph derivatives on lipid and sterol composition of candida albicans, cryptococcus neoformans, malassezia pachydermatis and malassezia furfur was investigated. the mics for the most effective derivatives were found in the range from 3.7 to 56.7 microm and were 2-3 times lower compared to the commercial fungicide bifonazol. the more efficient fenpropimorph derivatives were the piperidine derivative for c. albicans and th ... | 2001 | 11302214 |
| malassezia pachydermatis fungaemia in a neonatal intensive care unit. | malassezia pachydermatis, a non-obligatory lipophilic yeast, has occasionally been implicated in nosocomial fungaemias. this study investigated a cluster of eight cases of m. pachydermatis infection and colonization in a neonatal intensive care unit over a 6 mo period. all patients were preterm with very low birthweight and suffered from various underlying diseases. prolonged use of indwelling catheters and parenteral lipid formulations were important predisposing factors for their infection. al ... | 2001 | 11332175 |
| attachment of malassezia pachydermatis to the ear dermal cells in canine otitis externa. | to investigate the predominance of malassezia pachydermatis (m. pachydermatis) as a causative agent of canine otitis externa, ear cerumen samples were observed for adhesion of m. pachydermatis to the cornified epithelial cells by light and electron microscopes. the yeasts appeared not to adhere to the cornified epithelial cells directly, but they seemed to exist in the proximity of the epithelial cells with an electron opaque halo-like space around them. polysaccharide and lipid staining techniq ... | 2001 | 11459014 |
| the genetic diversity of clinical isolates of malassezia pachydermatis from dogs and cats. | molecular investigation of 110 clinical isolates of non-lipid-dependent malassezia pachydermatis from dogs and cats was carried out by random amplification of polymorphic dna (rapd) and chitin synthase 2 (chs2) gene sequence analyses. the rapd analysis indicated that the clinical isolates of m. pachydermatis constituted four distinct genetic types (a, b, c and d). moreover, the results from chs2 gene analysis completely agreed with those from the rapd analyses. the clinical isolates of m. pachyd ... | 2001 | 11556762 |
| response to malassezia pachydermatis by peripheral blood mononuclear cells from clinically normal and atopic dogs. | to investigate the potential cell-mediated immune response of atopic dogs to the yeast malassezia pachydermatis and to correlate it with the type-1 hypersensitivity (humoral) response of the same population of dogs. | 2002 | 11911571 |
| intradermal test reactivity to malassezia pachydermatis in atopic dogs. | | 2002 | 11993975 |
| susceptibility testing of malassezia pachydermatis using the urea broth microdilution method. | the in vitro susceptibility of 24 isolates of malassezia pachydermatis to four antifungal drugs in combination with lysozyme was determined using a urea broth microdilution method. the antifungal activities of each drug alone against 24 isolates of m. pachydermatis were determined as the mean minimal inhibitory concentrations (mics). mics of bifonazole, itraconazole, amorolfine and terbinafine were 3.2 microg ml(-1), 1.6 microg ml(-1), 25 microg ml(-1) and 3.2 microg ml(-1), respectively. lysozy ... | 2002 | 12000506 |
| homogeneous cell suspension of malassezia pachydermatis obtained with an ultrasonic homogenizer. | it is difficult to produce homogeneous cell suspensions of malassezia pachydermatis, since yeast cells paste up and form many clumps. however, homogeneous fungal suspensions are required for susceptibility examinations and biochemical analyses. although several types of trials have been carried out using glass homogenizers and many types of agents to obtain homogeneous fungal suspension. they have not yielded good results. we therefore attempted to use an ultrasonic homogenizer to separate clump ... | 2002 | 12014587 |
| immunoglobulin g responses to malassezia pachydermatis in healthy dogs and dogs with malassezia dermatitis. | serum immunoglobulin g (igg) responses of healthy dogs and dogs with malasseziapachydermatis dermatitis were compared by western immunoblotting. m pachydermatis cbs 1879 was disrupted mechanically and its proteins were separated and blotted on to nitrocellulose membranes before being incubated with sera from eight healthy beagles, eight irish setters with gluten-sensitive enteropathy, 15 healthy basset hounds, and 30 dogs with mpachydermatis-associated dermatitis, 20 of which were basset hounds. ... | 2002 | 12017525 |
| occurrence of malassezia spp. in the external ear canals of dogs and cats with and without otitis externa. | we studied the lipophilic microbiota of the external ear canals of 332 animals (264 dogs and 68 cats), with and without otitis externa, over an 11-year period from 1988 to 1999. malassezia pachydermatis was isolated from 62.2% and 50% of dogs with and without otitis externa, respectively, and from 41.2% and 17.6% of cats with and without otitis externa, respectively. in the group of animals studied for lipid-dependent species, these yeasts were isolated from 4.5% of dogs with otitis externa and ... | 2002 | 12058723 |
| antifungal effect of australian tea tree oil on malassezia pachydermatis isolated from canines suffering from cutaneous skin disease. | the lipophilic yeast malassezia pachydermatis is part of the normal skin flora of most warm-blooded organisms. in a number of surveys it could be demonstrated that this yeast species might be involved in different skin diseases like seborrhoeic dermatitis, especially in dogs and cats. in order to look for an alternative therapeutic agent to the commonly used antimycotic and antiseptic synthetic substances the in vitro activity of australian tea tree oil, the essential oil of melaleuca alternifol ... | 2002 | 12070905 |
| identification of major allergens of malassezia pachydermatis in dogs with atopic dermatitis and malassezia overgrowth. | we have previously shown that both atopic and normal dogs generate an igg response to antigens of malassezia pachydermatis. the aim of this study was to compare ige responses to separated proteins of m. pachydermatis in 28 atopic dogs with malassezia dermatitis and 22 clinically normal dogs using western immunoblotting. six different detection systems were evaluated in order to assess sensitivity and eliminate nonspecific binding and cross-reactivity. the protocol yielding the best results utili ... | 2002 | 12074703 |
| comparison of pulse administration versus once daily administration of itraconazole for the treatment of malassezia pachydermatis dermatitis and otitis in dogs. | objective to compare clinical efficacy of pulse administration with itraconazole versus once daily administration for the treatment of cutaneous and otic m pachydermatis infection in dogs. | 2002 | 12092953 |
| pathological and clinical aspects of the diseases caused by malassezia species. | from veterinary point of view malassezia pachydermatis has the greatest significance. it has been standing in the focus of interest since the early 1990s, mostly because of the frequency of otitis externa and dermatitis caused by this yeast in dogs. this is the only lipid-independent species in the genus malassezia. it can be found in very large proportion on the skin of healthy animals, but can be isolated in much greater number from diseased dogs. it often causes illness together with other pa ... | 2002 | 12109170 |