| cell-to-cell trafficking, subcellular distribution, and binding to coat protein of broad bean wilt virus 2 vp37 protein. | broad bean wilt virus 2 (bbwv 2) is a member of the genus fabavirus of the family comoviridae. to date, a movement protein (mp) of bbwv 2 has not been described. here we demonstrate that the green fluorescent protein (gfp)-vp37 fusion protein can move from initial bombarded cells to neighboring cells in nicotiana benthamiana epidermal leaves. in addition, the gfp-vp37 fusion protein localizes as a halo around the nucleus and as punctate spots on the cell periphery in n. benthamiana epidermal lea ... | 2009 | 19463725 |
| genetic compositions of broad bean wilt virus 2 infecting red pepper in korea. | the incidence of broad bean wilt virus 2 (bbwv2) on red pepper was investigated using the samples obtained from 24 areas of 8 provinces in korea. two hundred and five samples (79%) out of 260 collected samples were found to be infected with bbwv2. while the single infection rate of bbwv2 was 21.5%, the co-infection rate of bbwv2 with cucumber mosaic virus, pepper mottle virus, pepper mild mottle virus and/or potato virus y was 78.5%. to characterize the genetic diversity of bbwv2 korean isolates ... | 2013 | 25288954 |
| molecular characterization and variation of the broad bean wilt virus 2 isolates based on analyses of complete genome sequences. | the full-genome sequences of fourteen isolates of broad bean wilt virus 2 (bbwv2), collected from broad bean, pea, spinach, bell pepper and paprika plants in korea during the years 2006-2012, were determined and analyzed comparatively along with fifteen previously reported bbwv2 genome sequences. sequence analyses showed that rna-1 and rna-2 sequences of bbwv2 korean isolates consisted of 5950-5956 and 3568-3604 nucleotides, respectively. full-length genome sequence-based phylogenetic analyses r ... | 2013 | 25288968 |
| [molecular identification and sequence analysis of broad bean wilt virus 2 isolates from atractylodes macrocephala koidz]. | to identity the pathogen that causes the mosaic and yellowing symptoms on atractylodes macrocephala koidz in jiangxian, shanxi province, biological inoculation, sequence-independent amplification (sia),rt-pcr and other identification methods were used. the results showed that the chlorotic and necrosis symptoms occurred in the indicator plant chenopodium quinoa after it was infected with the pathogen,and the same symptoms appeared after the reinoculation of healthy atractylodes macrocephala koid ... | 2015 | 25997332 |
| a determinant of disease symptom severity is located in rna2 of broad bean wilt virus 2. | broad bean wilt virus 2 (bbwv2), which belongs to the genus fabavirus, is a destructive pathogen of many economically important horticultural and ornamental crops. in this study, we constructed infectious full-length cdna clones of two distinct isolates of bbwv2 under control of the cauliflower mosaic virus 35s promoter. bbwv2-pap1 isolated from paprika (capsicum annuum var. gulosum) induces severe disease symptoms in various pepper varieties, whereas bbwv2-rp1 isolated from red pepper (capsicum ... | 2016 | 26428303 |
| mutual association of broad bean wilt virus 2 vp37-derived tubules and plasmodesmata obtained from cytological observation. | the movement protein vp37 of broad bean wilt virus 2 (bbwv 2) forms tubules in the plasmodesmata (pd) for the transport of virions between cells. this paper reports a mutual association between the bbwv 2 vp37-tubule complex and pd at the cytological level as determined by transmission electron microscopy. the generation of vp37-tubules within different pd leads to a different occurrence frequency as well as different morphology lines of virus-like particles. in addition, the frequency of vp37-t ... | 2016 | 26903400 |
| near-complete genome sequence of grapevine fabavirus, a novel putative member of the genus fabavirus. | a novel virus-like sequence from grapevine was identified by illumina sequencing. the genomic organization was most similar to that of members of the genus fabavirus polyproteins rna-1 and rna-2 of the virus tentatively named grapevine fabavirus (gfabv) shared 34 to 23% sequence identities with broad bean wilt virus 2 (bbwv2), respectively. gfabv was successfully graft transmitted to vitis vinifera cv. cabernet franc. | 2016 | 27445385 |
| ecogenomic survey of plant viruses infecting tobacco by next generation sequencing. | the invasion of plant by viruses cause major damage to plants and reduces crop yield and integrity. devastating plant virus infection has been experienced at different times all over the world, which are attributed to different events of mutation, re-assortment and recombination occurring in the viruses. strategies for proper virus management has been mostly limited to eradicating the vectors that spreads the plant viruses. however, development of prompt and effective diagnostic methods are requ ... | 2016 | 27814723 |
| detection and identification of fabavirus species by one-step rt-pcr and multiplex rt-pcr. | the genus fabavirus of the family secoviridae comprises a group of poorly characterized viruses. to date, only five species have been described: broad bean wilt virus 1 (bbwv-1), broad bean wilt virus 2 (bbwv-2), lamium mild mosaic virus (lmmv), gentian mosaic virus (gemv) and cucurbit mild mosaic virus (cummv). the development is described of two rt-pcr procedures for the detection and identification of fabavirus species: a one-step rt-pcr using a single pair of conserved primers for the detect ... | 2014 | 24361876 |
| establishment of an agroinoculation system for broad bean wilt virus 2. | we determined the complete nucleotide sequence of a broad bean wilt virus 2 (bbwv-2) isolate from gentian in japan. the full-length rna1 and rna2 sequences, excluding poly(a) tails, were 5955 and 3600 nucleotides long, respectively. analysis indicated that, in contrast to other bbwv-2 isolates, the 5' end of both rna1 and rna2 starts with a guu sequence. we successfully inoculated nicotiana benthamiana with bbwv-2 by infiltrating a mixed suspension of two agrobacterium tumefaciens clones carryin ... | 2013 | 23404460 |
| gentian mosaic virus: a new species in the genus fabavirus. | abstract a viral isolate, designated n-1 and obtained from a gentian (gentiana scabra) plant that exhibited mosaic symptoms, was transmitted mechanically to nine plant species in six families. these plants are known as hosts of fabaviruses. the n-1 isolate was composed of isometric particles 30 nm in diameter and included two rna molecules of approximately 6.0 and 3.6 kb in length, as estimated by agarose gel electrophoresis. the rnas were encapsidated separately in two of the three types of par ... | 2005 | 18943990 |
| genome sequence and characterization of a new virus infecting mikania micrantha h.b.k. | the complete rna genomic sequence of a new virus infecting mikania micrantha, designated as mikania micrantha wilt virus (mmwv), has been determined. the genomic sequence and the predicted gene products of mmwv were similar to those of the other viruses of the genus fabavirus. the mmwv nucleotide sequence showed 75.6% identity to that of gentian mosaic virus, 56.6 and 57% identity to those of two broad bean wilt virus 1 isolates, and between 55.7 and 58% identity to those of seven broad bean wil ... | 2008 | 18679764 |
| in vivo accumulation of broad bean wilt virus 2 vp37 protein and its ability to bind single-stranded nucleic acid. | the vp37 protein encoded by the rna2 of broad bean wilt virus 2 (bbwv2) was overexpressed in escherichia coli. the protein was purified and a polyclonal antibody specific for the protein was produced. time course studies by western blot assays in bbwv2-infected chenopodium quinoa leaves showed that the vp37 protein was present in cells of the inoculated leaves by 12 h post inoculation and in cells of systemically-infected leaves by 2 days post inoculation. the protein was able to accumulate to a ... | 2002 | 12021864 |
| the vp37 protein of broad bean wilt virus 2 induces tubule-like structures in both plant and insect cells. | vp37 protein of broad bean wilt virus 2 (bbwv-2) is a multifunctional protein that binds single-strand nucleic acids, interacts with viral coat protein (cp) and potentiates the virus cell-to-cell movement in its host plant. in this study, tubule-like structures filled with virus-like particles were observed by electron microscopy in plasmodesmata in walls of chenopodium quinoa leaf cells infected with bbwv-2. immunogold labeling using vp37 protein specific antibody demonstrates that the vp37 is ... | 2011 | 20832435 |
| genetic variation and evolutionary analysis of broad bean wilt virus 2. | the genetic variation and evolutionary mechanisms of broad bean wilt virus 2 (bbwv-2) were studied by nucleotide sequence analysis of four genomic regions of 30 isolates from different countries. nucleotide diversity was high (0.198) for a plant virus. phylogenetic and genetic structure analyses showed low population subdivision, suggesting a significant gene flow between distant geographic areas. analysis of synonymous and nonsynonymous substitutions showed different negative selection pressure ... | 2011 | 21625974 |
| broad bean wilt virus 2 encoded vp53, vp37 and large capsid protein orchestrate suppression of rna silencing in plant. | viruses encode rna silencing suppressors to counteract host rna silencing-mediated defense responses. in this study, we demonstrate that vp53, vp37 and lcp encoded by rna2 of broad bean wilt virus 2 (bbwv-2), a member of the genus fabavirus, are strong suppressors of rna silencing triggered by single-stranded sense rna. they, however, had no effect on suppression of rna silencing induced by double-stranded rna. we provide evidence that these three suppressors can significantly limit the accumula ... | 2014 | 25173697 |
| [effects of broad bean wilt virus 2 isolate infection on photosynthetic activities and chloroplast ultrastructure in broad bean leaves]. | the effects of broad bean wilt virus 2 (bbwv 2) isolate b935 and pv131 infection on photosynthetic activities and chloroplast ultrastructure in broad bean (vicia faba) were investigated. as the disease progressed, net photosynthetic rate (p(n)), stomatal conductance (g(s)) of leaves, the chlorophyll content and chlorophyll a/b ratio decreased while the intercellular co(2) concentration (c(i)) of leaves rose in comparison with that of the healthy plants (figs. 1, 2). after bbwv 2 infection, f(v)/ ... | 2006 | 16957402 |
| complete nucleotide sequence and infectious cdna clone of the rna1 of a chinese isolate of broad bean wilt virus 2. | the nucleotide sequence of the rna1 of broad bean wilt virus 2 (bbwv2) isolate b935 has been determined from overlapping cdna clones. it contains 5956 nucleotides in length excluding the 3' terminal poly(a) tail and contains a single long open reading frame (orf) of 5613 nucleotides extending from nucleotide 234 to 5846. a repeated motif has been found in the 5' non-coding region. the predicted polyprotein encoded by the long orf is 1870 amino acid in length with a molecular weight of 210 k. ami ... | 2000 | 10949946 |
| nucleotide sequence and synthesis of infectious rna from cloned cdna of broad bean wilt virus 2 rna 2*. | the complete nucleotide sequence of broad bean wilt virus -2 (bbwv-2) rna 2 was determined. the sequence is 3593 nucleotides in length, and contains a single open reading frame which has the potential to encode a 119 kda polyprotein. direct amino acid sequencing of the virus coat proteins suggests that the polyprotein is processed to produce three proteins, 52 kda, 44 kda and 22 kda in size. a comparison of several fabaviruses in terms of nucleotide sequence and amino acid sequences showed that ... | 2000 | 10893156 |