| myroides marinus sp. nov., a member of the family flavobacteriaceae, isolated from seawater. | a gram-negative, aerobic, non-motile, yellow-pigmented, rod-shaped bacterium (strain js-08(t)) isolated from seawater was subjected to a polyphasic taxonomic study. 16s rrna gene sequence analysis indicated that strain js-08(t) belongs to the genus myroides, a member of the phylum bacteroidetes. its closest phylogenetic relative was myroides odoratimimus jcm 7460(t), with which it shared 97.0 % 16s rna gene sequence similarity. strain js-08(t) contained menaquinone-6 (mk-6) as the predominant me ... | 2011 | 20495024 |
| biosurfactants, bioemulsifiers and exopolysaccharides from marine microorganisms. | marine biosphere offers wealthy flora and fauna, which represents a vast natural resource of imperative functional commercial grade products. among the various bioactive compounds, biosurfactant (bs)/bioemulsifiers (be) are attracting major interest and attention due to their structural and functional diversity. the versatile properties of surface active molecules find numerous applications in various industries. marine microorganisms such as acinetobacter, arthrobacter, pseudomonas, halomonas, ... | 2010 | 20172021 |
| biofilm-forming capacity, surface hydrophobicity and aggregation characteristics of myroides odoratus isolated from south african oreochromis mossambicus fish. | although myroides spp. are primarily isolated from clinical sources, they have also been isolated from freshwater fish, stainless steel weldment and catheter biofilms. the ability of these nonmotile isolates to adhere to surfaces and the role of hydrophobicity and autoaggregation were investigated. | 2009 | 19519668 |
| ecological function of myroilysin, a novel bacterial m12 metalloprotease with elastinolytic activity and a synergistic role in collagen hydrolysis, in biodegradation of deep-sea high-molecular-weight organic nitrogen. | nearly all high-molecular-weight (hmw) dissolved organic nitrogen and part of the particulate organic nitrogen in the deep sea are present in hydrolysis-resistant amides, and so far the mechanisms of biodegradation of these types of nitrogen have not been resolved. the m12 family is the second largest family in subclan ma(m) of zn-containing metalloproteases and includes most enzymes from animals and only one enzyme (flavastacin) from a human-pathogenic bacterium (flavobacterium meningosepticum) ... | 2009 | 19201976 |
| biosynthesis of bile acids in a variety of marine bacterial taxa. | several marine bacterial strains, which were isolated from seawater off the island dokdo, korea, were screened to find new bioactive compounds such as antibiotics. among them, donghaeana dokdonensis strain dsw-6 was found to produce antibacterial agents, and the agents were then purified and analyzed by lc-ms/ms and 1d- and 2d-nmr spectrometries. the bioactive compounds were successfully identified as cholic acid and glycine-conjugated glycocholic acid, the 7alpha-dehydroxylated derivatives (deo ... | 2007 | 18050942 |
| cellulitis due to myroides odoratimimus in a patient with alcoholic cirrhosis. | | 2008 | 18039344 |
| oerskovia turbata and myroides species: rare isolates from a case of acalculus cholecystitis. | here we report a case of acalculus cholecystitis, which presented with features of obstructive jaundice of one-week duration. the patient underwent cholecystectomy and bile grew a mixed culture of oerskovia turbata and myroides spp. being a rare isolate, characteristic features of the former are described in this report. the patient recovered without any complication. | 2007 | 17901658 |
| myroides pelagicus sp. nov., isolated from seawater in thailand. | an aerobic, gram-negative, non-motile, yellow-to-orange pigmented and rod-shaped bacterium, designated strain sm1(t), was isolated from seawater in thailand and subjected to a polyphasic taxonomic study. phylogenetic analyses based on 16s rrna gene sequences revealed that the novel isolate shared 93-95 % sequence similarity with species of the genus myroides. the dna-dna relatedness values of strain sm1(t) with myroides odoratimimus jcm 7460(t) and myroides odoratus jcm 7458(t) were below 70 %. ... | 2006 | 16902031 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| gram-negative bacteria from patients seeking medical advice in stockholm after the tsunami catastrophe. | microbiological cultures from 229 patients seeking medical advice in stockholm after the tsunami catastrophe of december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative bacilli were the most common findings from wound cultures. common human pathogens such as escherichia coli, proteus species, klebsiella spp., and pseudomonas aeruginosa were isolated. more rare species of gram-negative bacilli, e.g. myroides odoratus, sp ... | 2006 | 16798691 |
| [rare bacteria species found in wounds of tsunami patients. predominance of gram-negative rods, increased antibiotic resistance]. | microbiological cultures from 229 patients seeking medical advise in stockholm after the tsunami catastrophe december 2004 were analysed at the clinical microbiology laboratory, karolinska university hospital, stockholm, sweden. gram-negative rods were the most common findings from wound cultures. common human pathogens as escherichia coli, proteus species, klebsiella spp, and pseudomonas aeruginosa were isolated. however, more rare species of gram-negative rods were also isolated, e.g. myroides ... | 2005 | 16416946 |
| a novel crude oil emulsifier excreted in the culture supernatant of a marine bacterium, myroides sp. strain sm1. | a marine bacterium, myroides sp. sm1, can grow on weathered crude oil and show emulsification of it. the biosurfactant able to emulsify crude oil was excreted in culture supernatant of myroides sp. sm1 grown on marine broth, which was extracted with chloroform/methanol (1:1) at ph 7 and purified by normal and reverse phase silica gel column chromatographies. the compound was ninhydrin-positive, and the chemical structure was elucidated by nuclear magnetic resonance (nmr), infrared spectroscopy ( ... | 2006 | 16059688 |
| ['in vitro' activity of different antimicrobial agents on gram-negative nonfermentative bacilli, excluding pseudomonas aeruginosa and acinetobacter spp]. | gram-negative nonfermentative bacilli (nfb) are widely spread in the environment. besides of difficulties for identification, they often have a marked multiresistance to antimicrobial agents, including those active against pseudomonas aeruginosa. the objective of this study was to evaluate the 'in vitro' activity of different antimicrobial agents on 177 gram-negative nonfermentative bacilli isolates (excluding pseudomonas aeruginosa and acinetobacter spp.) isolated from clinical specimens. minim ... | 2005 | 15991478 |
| chromosome-encoded beta-lactamases tus-1 and mus-1 from myroides odoratus and myroides odoratimimus (formerly flavobacterium odoratum), new members of the lineage of molecular subclass b1 metalloenzymes. | myroides odoratus and myroides odoratimimus (formerly designated in a single species as flavobacterium odoratum) are gram-negative aerobes and sources of nosocomial infections in humans. they have variable susceptibility to beta-lactams and a decreased susceptibility to carbapenems. using genomic dnas of m. odoratus cip 103105 and m. odoratimimus cip 103073 reference strains, shotgun cloning of beta-lactamase genes was performed, followed by protein expression in escherichia coli. the deduced am ... | 2002 | 12384365 |
| myroides odoratus cellulitis and bacteremia: case report and review. | a case of myroides odoratus cellulitis with bacteremia in an apparently immunocompetent man is presented. although common in soil and water, this agent is a rare clinical isolate and is often not considered pathogenic. the virulence of m. odoratus may be greater than is currently believed and it should be considered in bacteremias from cutaneous sources in immunocompetent patients. | 2001 | 11868770 |
| the chryseobacterium meningosepticum pafa enzyme: prototype of a new enzyme family of prokaryotic phosphate-irrepressible alkaline phosphatases? | chryseobacterium meningosepticum is an aerobic gram-negative rod widely distributed in natural environments. unlike many bacteria, it produces a phosphate-irrepressible periplasmic alkaline phosphatase (ap). this work describes cloning of the gene encoding that enzyme from c. meningosepticum ccug 4310 (nctc 10585), and preliminary characterization of its product. the gene, named pafa, encodes a protein (pafa) of 546 amino acids with a calculated molecular mass of the mature peptide of 58682 da. ... | 2001 | 11577161 |
| polyamine distribution profiles in newly validated genera and species within the flavobacterium-flexibacter-cytophaga-sphingobacterium complex. | cellular polyamines of 58 strains belonging to the flavobacterium-flexibacter-cytophaga-sphingobacterium complex were analysed by hplc. homospermidine was found in all species of flavobacterium, chryseobacterium, empedobacter, myroides, cellulophaga, salegentibacter, psychroserpens and gelidibacter of the family flavobacteriaceae. flavobacterium ferrugineum located outside of this family also contained homospermidine. cytophaga fermentans and c. xylanolytica belonging to the family bacteroidacea ... | 2001 | 11548199 |
| molecular typing of myroides odoratimimus (flavobacterium odoratum) urinary tract infections in a turkish hospital. | | 2000 | 11057514 |
| gut bacteria may be involved in interactions between plants, herbivores and their predators: microbial biosynthesis of n-acylglutamine surfactants as elicitors of plant volatiles. | n-acylamino acids are dominant and widespread constituents of insect oral secretions (regurgitants), serving the insect as biosurfactants in the digestive process. during feeding the conjugates may be introduced into damaged leaves and contribute there to the elicitation of plant defenses such as the induction of volatile biosynthesis. from gut segments of spodoptera exigua, mamestra brassicae and agrotis segetum 23 bacterial strains were isolated, ten of which were able to synthesise typical le ... | 2000 | 11030433 |
| psychrobacters and related bacteria in freshwater fish. | three phenotypic identification systems were employed to identify 106 strains of gram-negative, nonmotile, aerobic bacteria obtained during iced storage of wild (salmo trutta and esox lucius) and farmed (oncorhynchus mykiss) freshwater fish. using diagnostic tables and computer-assisted identification, the isolates were psychrobacter (64 strains), acinetobacter (24 strains), moraxella (6 strains), chryseobacterium (5 strains), myroides odoratus (2 strains), flavobacterium (1 strain), empedobacte ... | 2000 | 10716558 |
| myroides profundi sp. nov., isolated from deep-sea sediment of the southern okinawa trough. | a gram-negative, nonmotile, aerobic and oxidase- and catalase-positive bacterium, designated d25t, was isolated from the deep-sea sediments of the southern okinawa trough area. phylogenetic analyses of 16s rrna gene sequences showed that strain d25t fell within the genus myroides, with 99.2%, 96.0% and 93.4% sequence similarities to the only three recognized species of myroides. however, the dna-dna similarity value between strain d25t and its nearest neighbour myroides odoratimimus jcm 7460t wa ... | 2008 | 18684121 |
| investigation of an outbreak of central venous catheter-associated bloodstream infection due to contaminated water. | an outbreak of central venous catheter-associated bloodstream infections was reported in a hospital in ecuador. commercially produced ampoules of water for injection were found to be contaminated with burkholderia cepacia and myroides odoratus. removal of these ampoules yielded a 10-fold reduction in the incidence of catheter-associated infection. | 2008 | 18462150 |
| bile acids are new products of a marine bacterium, myroides sp. strain sm1. | strain sm1 was isolated as a biosurfactant-producing microorganism from seawater and presumptively identified as myroides sp., based on morphology, biochemical characteristics and 16s rdna sequence. the strain produced surface-active compounds in marine broth, which were purified, using emulsification activity for n-hexadecane as an indicator. the purified compounds were identified by thin-layer chromatography, (1)h- and (13)c-nmr spectra and fast atom bombardment mass spectrometry as cholic aci ... | 2005 | 15549287 |
| the incidence of weeksella- and bergeyella-like bacteria in the food environment. | eighty-five catalase- and oxidase-positive gram-negative rods and cocci susceptible to penicillin g were isolated from a variety of food sources. the phenotypic relationships of these isolates with reference cultures of bergeyella-like, chryseobacterium, empedobacter, myroides, moraxella, sphingobacterium and weeksella-like strains were examined by numerical taxonomy. seventy-three isolates were recovered in five groups; 80% of the isolates clustered in groups 1, 2 and 3 and produced indole, bea ... | 1998 | 9721639 |
| reappraisal of the antimicrobial susceptibilities of chryseobacterium and flavobacterium species and methods for reliable susceptibility testing. | several flavobacterium species, comprising a heterogeneous group of gram-negative bacilli that are capable of causing opportunistic infections in humans, have recently been reclassified as chryseobacterium or myroides species. intrinsically resistant to a number of antibiotics, these organisms have been reported to be susceptible to vancomycin and certain other drugs that are normally active against gram-positive bacteria. by using the national committee for clinical laboratory standards (nccls) ... | 1997 | 9420049 |
| 16s rdna sequence analysis of culturable marine biofilm forming bacteria from a ship's hull. | marine bacteria from the hull of a ship in the form of biofilms or microfouling were isolated, cultured, and identified by phylogenetic analysis using 16s rdna sequences. with an average length of 946 bp, all the 16 sequences were classified using the ribosomal database project (rdp) and were submitted to the national center for biotechnology information. phylogenetic analysis using 16s rdna sequences indicated that the 16 strains belonged to the firmicutes (ik-mb6 exiguobacterium aurantiacum, i ... | 2010 | 21038149 |
| septic shock, pneumonia, and soft tissue infection due to myroides odoratimimus: report of a case and review of myroides infections. | the genus myroides comprises aerobic, yellow-pigmented, non-motile, non-fermenting gram-negative rods formerly classified as flavobacterium odoratum. members of the genus are widely distributed in the environment, especially in water, and usually behave as low-grade opportunistic pathogens, having been found to cause urinary tract infection, endocarditis, ventriculitis, and cutaneous infections in severely immunocompromised patients. we report a case of soft tissue infection, septic shock, and p ... | 2011 | 21246247 |
| myroides phaeus sp. nov., isolated from human saliva and emended description of the genus myroides and the species m. profundi zhang et al. 2008 and m. marinus cho et al. in press. | a novel bacterial strain, designated my15(t), was isolated from saliva sample of a student during a teaching experiment in china. phylogenetic analyses based on 16s rrna gene sequences showed that the novel strain was most closely related to myroides marinus js-08(t), myroides odoratimimus lmg 4092(t) and myroides profundi d25(t) with 96.5 %, 96.3 % and 96.1 % sequence similarities, respectively, demonstrating that the novel strain belonged to the genus myroides. strain my15(t) formed pale yello ... | 2011 | 21571938 |
| genetic diversity and phylogeny of antagonistic bacteria against phytophthora nicotianae isolated from tobacco rhizosphere. | the genetic diversity of antagonistic bacteria from the tobacco rhizosphere was examined by boxair-pcr, 16s-rflp, 16s rrna sequence homology and phylogenetic analysis methods. these studies revealed that 4.01% of the 6652 tested had some inhibitory activity against phytophthora nicotianae. boxair-pcr analysis revealed 35 distinct amplimers aligning at a 91% similarity level, reflecting a high degree of genotypic diversity among the antagonistic bacteria. a total of 25 16s-rflp patterns were iden ... | 2011 | 21686169 |
| Nosocomial outbreak of Myroides odoratimimus urinary tract infection in a Tunisian hospital. | We report a nosocomial outbreak of urinary tract infection caused by Myroides odoratimimus, previously called Flavobacterium odoratum, in the urology unit of a Tunisian hospital. From May to November 2010, seven isolates of M. odoratimimus were recovered from urine. Pulsed-field gel electrophoresis clearly differentiated these isolates into two possibly related clones from two different periods. All patients but one had urinary calculi and underwent endourological surgery. All Myroides isolates ... | 2012 | 22099498 |
| identification of bacterial microflora in the midgut of the larvae and adult of wild caught anopheles stephensi: a step toward finding suitable paratransgenesis candidates. | to describe the midgut microbial diversity and to find the candidate bacteria for the genetic manipulation for the generation of paratransgenic anopheline mosquitoes refractory to transmission of malaria, the microbiota of wild larvae and adult anopheles stephensi mosquito midgut from southern iran was studied using a conventional cell-free culture technique and analysis of a 16s ribosomal rna (rrna) gene sequence library. forty species in 12 genera including seven gram-negative myroides, chryse ... | 2012 | 22074685 |