| characterization of malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (pfge). | a total of 220 isolates of the genus malassezia obtained from affected skin of animals and humans were included in this study. the isolates were examined for their belonging to the known species malassezia pachydermatis, malassezia sympodialis, malassezia furfur, malassezia obtusa, malassezia restricta, malassezia globosa and malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (tween 20, 40, 60, 80). t ... | 1999 | 10536433 |
| [taxonomy of malassezia furfur: state of the art]. | malassezia furfur is a lipophilic yeast considered as a normal component of the human skin flora. apart from pityriasis versicolor, m. furfur has been linked to several skin diseases such as seborrheic dermatitis, folliculitis or atopic dermatitis. moreover, these yeasts have been reported as agent of invasive human diseases including pneumonia, catheter-associated sepsis and peritonitis. the existence of morphological, serological, metabolical, biochemical and karyotipical differences has been ... | 1997 | 15538815 |
| prevalence of malassezia spp. in healthy pigs. | in the present study, we evaluated the occurrence of malassezia spp. from superficial areas (ear, anus, forehead and back) in 100 healthy pigs. all cultures from anus, forehead and back were negative for malassezia spp. a total of 73% of pigs were colonized by malassezia yeasts in the external ear canals. we isolated only lipid-dependent species (malassezia sympodialis 58%, malassezia slooffiae 30%). this study evaluates the occurrence of malassezia spp. in pigs since the taxonomic revision in 1 ... | 2005 | 15679660 |
| malassezia and candida colonisation on glans penis of circumcised men. | the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ... | 2005 | 16115108 |
| effect of circumcision on genital colonization of malassezia spp. in a pediatric population. | this study aimed to determine the prevalence of yeast fungi in samples from the glans penis and prepuce of circumcised and uncircumcised children. impression preparations were made on modified dixon and leeming-notman agar without cycloheximide. the isolates were identified by morphological and physiological characteristics. the yeasts were detected in 32 (28.6%) of 112 children, 12 (18.2%) being among 66 circumcised and 20 (43.5%) among 46 uncircumcised children. the most common species was mal ... | 2006 | 16519013 |
| malassezia slooffiae-associated dermatitis in a goat. | an adult male pygmy goat with a history of losing hair and declining body condition was euthanized, and a complete diagnostic work-up was performed. the animal showed diffuse alopecia on the dorsal and lateral sides of thorax and abdomen, proximal legs, neck and face. histology revealed diffuse orthokeratotic hyperkeratosis, epidermal hyperplasia and perivascular dermatitis. broad-based budding yeasts and hyphae were visible in the keratin layer. malassezia slooffiae was identified in the skin b ... | 2007 | 17845623 |
| [concordance between phenotypical features and pcr-rea for the identification of malassezia spp]. | the genus malassezia has been recently revised and nowadays includes 11 species that cannot always be differentiated from each other by physiological and morphological tests. this study was aimed to evaluate the correlation between a molecular method and conventional phenotypic features in the identification of malassezia spp. to achieve this aim, 92 argentinean clinical strains isolated between 2001 and 2005 were analyzed along with three reference strains (malassezia furfur cbs 7019, malassezi ... | 2007 | 18095760 |
| [isolation and identification of malassezia spp. in pytiriasis versicolor, seborrheic dermatitis and healthy skin.]. | the taxonomy of the genus malassezia has been recently revised and enlarged to include seven distinct species. the aim of the study was to analyse the prevalence of these species in the cutaneous lesions of pityriasis versicolor and dermatitis seborrhoeica, as well as in the normal skin. seventy-five patients with pityriasis versicolor, 75 of dermatitis seborrhoeica, and 150 samples of normal skin (75 from the forehead and 75 from the shoulders) were studied. a direct microscopy with koh + parke ... | 1999 | 18473598 |
| in vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of malassezia species. | malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. we hypothesize that the lipid-rich capsule of malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. to test this, preparations of capsulated or acapsular [10% (v/v) triton x-100 treated], viable and nonviable, exponential or stationary phase malassezia furfur, malassezia globosa, malassezia obtusa, malassezia restricta, malassezia slooffiae ... | 2008 | 18752620 |
| rapd differentiation of malassezia spp. from cattle, dogs and humans. | the molecular characterization of malassezia spp. isolates from animals and humans has not been thoroughly studied. we have analysed the dna profile by random amplified polymorphic dna (rapd)-pcr to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. the analysis of electrophoretic profiles on 8% polyacrylamide gel and their phenograms showed genetic heterogeneity between rapd profiles of malassezia furfur and malassezia slooffiae isolates from human ... | 2010 | 19298360 |
| distribution of malassezia species in seborrhoeic dermatitis: correlation with patients' cellular immune status. | malassezia species are implicated in the pathogenesis of seborrhoeic dermatitis (sd), but the relationship between each species and the disorder remains unclear. it is hypothesised that the pathogenesis of sd has an immune component, which is supported by the increased incidence in patients with immunosuppressive disorders. the purpose of our study was to analyse the prevalence of malassezia species in lesional skin of sd, and to assess the distribution of the species according to severity of th ... | 2010 | 19486300 |
| epidemiology of pityriasis versicolor in adana, turkey. | pityriasis versicolor is a common superficial mycoses of the skin. it is now recognized that the causative organisms of this infection are different species of malassezia. the aim of this study was to determine the distribution of malassezia species in patients with pityriasis versicolor in adana, turkey. in total, 97 patients positive for malassezia elements, namely, yeast cells and short hyphae in microscopic examination, were included in the study. all samples were inoculated in plates contai ... | 2009 | 19583685 |
| human external ear canal as the specific reservoir of malassezia slooffiae. | the incidence of malassezia species recovered from the external ear canal was characterized using culture medium optimized for malassezia spp., chromagar malassezia. the results of this study indicated that in healthy individuals m. slooffiae was the dominant malassezia species followed by m. restricta. | 2010 | 20105102 |
| prevalence of malassezia species in pityriasis versicolor lesions in northeast argentina. | malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens. | 2010 | 20346299 |
| identification of malassezia species from tunisian patients with pityriasis versicolor. | pityriasis versicolor is caused by malassezia sp. it is a common worldwide mycosis. recently, eleven species are known of the malassezia genus, and are identified in vitro by their morphological characteristics, biochemical tests and by molecular biology. the aim of this study is the identification of malassezia species from tunisian patients with pityriasis versicolor. | 2010 | 20415164 |
| [pityriasis versicolor: isolation and identification of the main species of malassezia]. | species of the genus malassezia isolated were: malassezia sympodialis (16.66%), malassezia furfur (12.50%), malassezia globosa (11.45%), and malassezia slooffiae (2.10%). malassezia sympodialis predominated in the study. the species of malassezia identified did not show correlation with clinical variants and with the distribution of pityriasis versicolor lesions in relation to areas of the body. | 2010 | 20464101 |
| prevalencia de especies de malassezia en pacientes con pitiriasis versicolor en rosario, argentina. | malassezia species are considered opportunistic yeasts of increasing clinical importance. these lipophilic yeasts are associated with various human diseases, especially pityriasis versicolor (pv), a chronic superficial scaling dermatomycosis. the aim of this study was to isolate, identify and analyze the distribution of the different species of malassezia in patients with pv in rosario city (argentina). a total of 264 clinical samples were studied. isolates were identified on the basis of micros ... | 2011 | 21419859 |
| extracellular phospholipase activity of malassezia spp. isolated from individuals with and without dermatological disease. | background: the malassezia genus includes mainly lipophilic yeasts belonging to the cutaneous microbiota of man and other mammals. some malassezia species have been associated with various dermatological diseases. the factors permitting the transformation of yeasts of the malassezia genus from a commensal organism to a pathogenic agent are still little known but the production of various enzymes such as lipase, phospholipase and lipoxygenase could contribute to the pathogenic activity of these y ... | 2011 | 21645629 |
| identification of malassezia yeast species isolated from patients with pityriasis versicolor. | pityriasis versicolor (pv) is a disease with worldwide distribution. twelve different species of malassezia yeast have been described. the objective of this study was to determine which species of malassezia are more prevalent in patients with pityriasis versicolor. samples were collected by scraping the lesions of 87 patients with a clinical suspicion of pityriasis versicolor. the samples were then submitted to fungal microscopy and culture to identify the species. the species found were: malas ... | 2011 | 21987156 |
| malassezia species in healthy skin and in dermatological conditions. | the genus malassezia comprises lipophilic species, the natural habitat of which is the skin of humans and other warm-blooded animals. however, these species have been associated with a diversity of dermatological disorders and even systemic infections. pityriasis versicolor is the only cutaneous disease etiologically connected to malassezia yeasts. in the other dermatoses, such as malassezia folliculitis, seborrheic dermatitis, atopic dermatitis, and psoriasis, these yeasts have been suggested t ... | 2016 | 26710919 |
| detection and identification of malassezia species in domestic animals and aquatic birds by pcr-rflp. | the present study aimed at detection and species-level identification of the malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds and 5 turkeys. tape-strip samples were examined by direct microscopy. all samples were inoculated on mod ... | 2015 | 27175148 |
| in vitro antifungal activity of topical and systemic antifungal drugs against malassezia species. | the strict nutritional requirements of malassezia species make it difficult to test the antifungal susceptibility. treatments of the chronic and recurrent infections associated with malassezia spp. are usually ineffective. the objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of malassezia species against 16 antifungal drugs used for topical or systemic treatment. isolates were identified by restriction fragment length polymorphism. minimal inhibitory ... | 2013 | 23496653 |
| is malassezia nana the main species in horses' ear canal microbiome? | the objective of this study was to characterize genotypically malassezia spp. isolated from the external ear canal of healthy horses. fifty-five horses, 39 (70.9%) males and 16 (29.1%) females, from different breeds and adults were studied. external ear canals were cleaned and a sterile cotton swab was introduced to collect cerumen. a total of 110 samples were cultured into dixon medium and were incubated at 32°c for up to 15 days. macro- and micromorphology and phenotypic identification were pe ... | 2017 | 27287335 |
| comprehensive analysis of the fungal microbiota of the human external auditory canal using pyrosequencing. | we previously reported that the malassezia microbiota of the external auditory canal were composed principally of malassezia slooffiae and malassezia restricta. in the present study, the fungal microbiota of the external auditory canal were comprehensively analyzed by pyrosequencing on an flx-454 platform. of 17 samples tested, the fungal microbiota of 14 samples were composed of > 90% malassezia species; either m. slooffiae or m. restricta was abundant in individual samples. the external audito ... | 2017 | 28250358 |