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fast, noninvasive method for molecular detection and differentiation of malassezia yeast species on human skin and application of the method to dandruff microbiology.malassezia fungi have been the suspected cause of dandruff for more than a century. previously referred to as pityrosporum ovale, pityrosporum orbiculare, or malassezia, these fungi are now known to consist of at least seven malassezia species. each species has a specific ecological niche, as well as specific biochemical and genetic characteristics. malassezia yeasts have fastidious culture conditions and exceedingly different growth rates. therefore, the results of surveys of malassezia based o ...200212202578
genotype analysis of malassezia restricta as the major cutaneous flora in patients with atopic dermatitis and healthy subjects.lipophilic yeasts of the genus malassezia colonize the skin surface of humans and are an exacerbating factor in atopic dermatitis (ad). two species, m. restricta and m. globosa are major cutaneous microflora in both ad patients and healthy subjects. we compared the dna sequences of the intergenic spacer (igs) region, located between the 26s and 5s rrna genes of m. restricta colonizing the skin surfaces of 13 ad patients and 12 healthy subjects, and of three cbs stock strains as references. the i ...200415502408
quantitative investigation on the distribution of malassezia species on healthy human skin in korea.this study concerns a culture research based on the data gathered from korean subjects to examine distribution of malassezia yeast. malassezia yeast were cultivated out of samples from scalp, forehead, chest, arm and thigh. malassezia restricta was recovered more frequently in the teens and young adults, while m. globosa was the predominant species in subjects older than 50 years of age. the population density of malassezia yeast was significantly higher in the age group (ag) of 21-30 years comp ...200616922793
dandruff-associated malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens.fungi in the genus malassezia are ubiquitous skin residents of humans and other warm-blooded animals. malassezia are involved in disorders including dandruff and seborrheic dermatitis, which together affect >50% of humans. despite the importance of malassezia in common skin diseases, remarkably little is known at the molecular level. we describe the genome, secretory proteome, and expression of selected genes of malassezia globosa. further, we report a comparative survey of the genome and secret ...200718000048
[isolation and identification of malassezia spp. in pytiriasis versicolor, seborrheic dermatitis and healthy skin.].the taxonomy of the genus malassezia has been recently revised and enlarged to include seven distinct species. the aim of the study was to analyse the prevalence of these species in the cutaneous lesions of pityriasis versicolor and dermatitis seborrhoeica, as well as in the normal skin. seventy-five patients with pityriasis versicolor, 75 of dermatitis seborrhoeica, and 150 samples of normal skin (75 from the forehead and 75 from the shoulders) were studied. a direct microscopy with koh + parke ...199918473598
in vitro antifungal activities of luliconazole, a new topical imidazole.luliconazole is a topical antifungal drug newly developed in japan. the present study compares the in vitro antifungal activity of luliconazole against clinically important dermatomycotic fungi with that of other representative antifungal drugs. the reference drugs chosen were five classes of nine topical agents, i.e., allylamine (terbinafine), thiocarbamate (liranaftate), benzylamine (butenafine), morpholine (amorolfine), and azole (ketoconazole, clotrimazole, neticonazole, miconazole and bifon ...200919115136
identification of malassezia yeast species isolated from patients with pityriasis versicolor.pityriasis versicolor (pv) is a disease with worldwide distribution. twelve different species of malassezia yeast have been described. the objective of this study was to determine which species of malassezia are more prevalent in patients with pityriasis versicolor. samples were collected by scraping the lesions of 87 patients with a clinical suspicion of pityriasis versicolor. the samples were then submitted to fungal microscopy and culture to identify the species. the species found were: malas ...201121987156
distribution of malassezia species in seborrhoeic dermatitis: correlation with patients' cellular immune status.malassezia species are implicated in the pathogenesis of seborrhoeic dermatitis (sd), but the relationship between each species and the disorder remains unclear. it is hypothesised that the pathogenesis of sd has an immune component, which is supported by the increased incidence in patients with immunosuppressive disorders. the purpose of our study was to analyse the prevalence of malassezia species in lesional skin of sd, and to assess the distribution of the species according to severity of th ...201019486300
plant and fungal diversity in gut microbiota as revealed by molecular and culture investigations.few studies describing eukaryotic communities in the human gut microbiota have been published. the objective of this study was to investigate comprehensively the repertoire of plant and fungal species in the gut microbiota of an obese patient.201323555039
eukaryote culturomics of the gut reveals new species.the repertoire of microeukaryotes in the human gut has been poorly explored, mainly in individuals living in northern hemisphere countries. we further explored this repertoire using pcr-sequencing and culture in seven individuals living in four tropical countries. a total of 41 microeukaryotes including 38 different fungal species and three protists were detected. four fungal species, davidiella tassiana, davidiella sp., corticiaceae sp., and penicillium sp., were uniquely detected by culture; 2 ...201425210972
molecular characterization of fungal populations on the tongue dorsum of institutionalized elderly adults.to characterize the global composition of oral fungal populations in frail elderly adults and to investigate the relationship with their health status.201222642872
[taxonomy of malassezia furfur: state of the art].malassezia furfur is a lipophilic yeast considered as a normal component of the human skin flora. apart from pityriasis versicolor, m. furfur has been linked to several skin diseases such as seborrheic dermatitis, folliculitis or atopic dermatitis. moreover, these yeasts have been reported as agent of invasive human diseases including pneumonia, catheter-associated sepsis and peritonitis. the existence of morphological, serological, metabolical, biochemical and karyotipical differences has been ...199715538815
microorganisms in the gut of beetles: evidence from molecular cloning.we have regularly cultured yeasts from the gut of certain beetles in our ongoing research. in this study cloned pcr products amplified from the gut contents of certain mushroom-feeding and wood-ingesting beetles in four families (erotylidae, tenebrionidae, ciidae, and passalidae) were sequenced and compared with culture results. cultural techniques detected some yeasts present in the gut of the beetles, including a pichia stipitis-like yeast associated with wood-ingesting passalid beetles. clone ...200314726245
characterization of malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (pfge).a total of 220 isolates of the genus malassezia obtained from affected skin of animals and humans were included in this study. the isolates were examined for their belonging to the known species malassezia pachydermatis, malassezia sympodialis, malassezia furfur, malassezia obtusa, malassezia restricta, malassezia globosa and malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (tween 20, 40, 60, 80). t ...199910536433
extracellular phospholipase activity of malassezia spp. isolated from individuals with and without dermatological disease.background: the malassezia genus includes mainly lipophilic yeasts belonging to the cutaneous microbiota of man and other mammals. some malassezia species have been associated with various dermatological diseases. the factors permitting the transformation of yeasts of the malassezia genus from a commensal organism to a pathogenic agent are still little known but the production of various enzymes such as lipase, phospholipase and lipoxygenase could contribute to the pathogenic activity of these y ...201121645629
fungal diversity in composting process of pig manure and mushroom cultural waste based on partial sequence of large subunit rrna.fungal diversity during composting was investigated by culture-independent rdna sequence analysis. composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (hazaka system), and samples were collected at various stages. based on partial sequence analysis of large subunit (lsu) ribosomal rna (rrna) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; geotrichum sp., debaryomyces hansenii, monographella ni ...200919734710
[animal model for superficial mycosis].tinea corporis and the tinea pedis model in guinea pig with trichophyton mentagrophytes are well established models of dermatophytoses. we attempted to provide animal infection models for t. tonsurans, endemic in japan, and malassezia restricta, an important pathogenic factor in seborrhoeic dermatitis, by utilizing the tinea corporis model. an inoculum of the organisms was applied to the back skin of male guinea pigs. t. tonsurans infected animals showed follicular inflammation mimicking those s ...200919430182
evaluation of the levels of specific ige against cryptococcus diffluens and cryptococcus liquefaciens in patients with atopic dermatitis.cryptococcus diffluens and cryptococcus liquefaciens, 2 basidiomycetous yeasts, frequently colonize the skin of patients with atopic dermatitis (ad). in this study, we investigated the presence of specific ige antibodies against c. diffluens and c. liquefaciens in the sera of ad patients by using an enzyme immunoassay . of the 122 ad serum samples tested, 43 (35.2%) and 50 (41.0%) were positive for specific ige antibodies against c. diffluens and c. liquefaciens, respectively. the levels of spec ...200717951984
antifungal and antioxidant activities of the phytomedicine pipsissewa, chimaphila umbellata.bioassay-guided fractionation of chimaphila umbellata (l.) w. bart (pyrolaceae) ethanol extracts led to the identification of 2,7-dimethyl-1,4-naphthoquinone (chimaphilin) as the principal antifungal component. the structure of chimaphilin was confirmed by 1h and 13c nmr spectroscopy. the antifungal activity of chimaphilin was evaluated using the microdilution method with saccharomyces cerevisiae (0.05mg/ml) and the dandruff-associated fungi malassezia globosa (0.39mg/ml) and malassezia restrict ...200817950387
keratitis caused by a rare fungus, malassezia restricta.malassezia species rarely cause keratitis, and, thus, the clinical manifestations of malassezia keratitis are not well known.200717660990
cytokine secretion profile of human keratinocytes exposed to malassezia yeasts.the lipophilic yeast malassezia is an exacerbating factor in atopic dermatitis (ad). among organisms of the malassezia species, malassezia globosa and malassezia restricta are particularly dominant on the skin of ad patients. however, the precise role of malassezia yeasts in the pathophysiology of ad remains uncertain. keratinocytes play a critical role in cutaneous inflammatory and immune responses by secreting cytokines. in this study, we attempted to determine the cytokine secretion profiles ...200617069617
the role of sebaceous gland activity and scalp microfloral metabolism in the etiology of seborrheic dermatitis and dandruff.most common scalp flaking disorders show a strong correlation with sebaceous gland (sg) activity. early sg activity in the neonate results in microfloral colonization and cradle cap. after maternal hormonal control subsides, there is little sg activity until puberty, when the sg turns on under sex hormone control. when the sg activity increases, the present but low malassezia population has a new food source and proliferates, resulting in the scalp itching and flaking common to greater than 50% ...200516382662
antifungal activities of tacrolimus and azole agents against the eleven currently accepted malassezia species.the lipophilic yeast malassezia is an exacerbating factor in atopic dermatitis (ad) and colonizes the skin surface of patients with ad. with the goal of reducing the number of malassezia cells, we investigated the antifungal activities of a therapeutic agent for ad, tacrolimus, and the azole agents itraconazole and ketoconazole against malassezia species in vitro. we examined 125 strains of the 11 currently accepted malassezia species by using the agar dilution method. all strains of the 11 mala ...200515956404
prevalence of malassezia species in pityriasis versicolor lesions in northeast argentina.malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens.201020346299
in vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of malassezia species.malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. we hypothesize that the lipid-rich capsule of malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. to test this, preparations of capsulated or acapsular [10% (v/v) triton x-100 treated], viable and nonviable, exponential or stationary phase malassezia furfur, malassezia globosa, malassezia obtusa, malassezia restricta, malassezia slooffiae ...200818752620
distribution of malassezia species in patients with psoriasis and healthy individuals in tehran, iran.psoriasis is a non-contagious disorder that affects the skin as red scaly patches. although the role of malassezia species in the pathogenesis of psoriasis is still not fully understood, it is thought that these lipophilic yeasts might be a trigger factor in the exacerbation of psoriatic lesions.200818616765
analysis of malassezia microbiota in healthy superficial human skin and in psoriatic lesions by multiplex real-time pcr.yeasts from the genus malassezia are members of the normal biota of human skin, and may play a role in dermatopathology. our previous study of the fungal microbiota from healthy subjects and from patients with psoriasis using clone library analysis revealed the presence of five malassezia species and four uncharacterized phylotypes. we now compared the malassezia microbiota from six healthy body locations and two psoriatic lesions, and evaluated its stability over time using multiplex real-time ...200818294199
cutaneous malassezia flora in atopic dermatitis differs between adults and children.malassezia species are suspected to be involved in the development of skin lesions in atopic dermatitis (ad) when the response of adult ad to anti-inflammatory treatments is poor. however, a comparative analysis of malassezia flora between adults and children with ad has not been performed.200717916215
quantitative analysis of malassezia in the scale of patients with psoriasis using a real-time polymerase chain reaction assay.it has been suggested that malassezia is associated with the development of skin lesions in psoriasis because of the response of the scalp lesions in psoriasis to antifungal agents. malassezia restricta and m. globosa are the two major members of the cutaneous malassezia flora in patients with psoriasis, although they have not been analysed quantitatively.200717634085
isolation and expression of a malassezia globosa lipase gene, lip1.dandruff and seborrheic dermatitis (d/sd) are common hyperproliferative scalp disorders with a similar etiology. both result, in part, from metabolic activity of malassezia globosa and malassezia restricta, commensal basidiomycete yeasts commonly found on human scalps. current hypotheses about the mechanism of d/sd include malassezia-induced fatty acid metabolism, particularly lipase-mediated breakdown of sebaceous lipids and release of irritating free fatty acids. we report that lipase activity ...200717460728
in vitro antifungal efficacy of ciclopirox olamine alone and associated with zinc pyrithione compared to ketoconazole against malassezia globosa and malassezia restricta reference strains.the aim of this study was to determine the in vitro fungicidal and growth inhibitory activity of ciclopirox olamine alone (1% and 1.5%) or in association with 1% zinc pyrithione compared to 2% ketoconazole, against malassezia species particularly involved in the pathogenesis of seborrheic dermatitis. experiments were performed on malassezia globosa ip 2387.96 and m. restricta ip 2392.96 strains. growth inhibitory activity of the active compounds in solution was evaluated by measuring minimal inh ...200617146583
whole genome sequencing analysis of the cutaneous pathogenic yeast malassezia restricta and identification of the major lipase expressed on the scalp of patients with dandruff.malassezia species are opportunistic pathogenic fungi that are frequently associated with seborrhoeic dermatitis, including dandruff. most malassezia species are lipid dependent, a property that is compensated by breaking down host sebum into fatty acids by lipases. in this study, we aimed to sequence and analyse the whole genome of malassezia restricta kctc 27527, a clinical isolate from a korean patient with severe dandruff, to search for lipase orthologues and identify the lipase that is the ...201727882618
dandruff is associated with disequilibrium in the proportion of the major bacterial and fungal populations colonizing the scalp.the bacterial and fungal communities associated with dandruff were investigated using culture-independent methodologies in the french subjects. the major bacterial and fungal species inhabiting the scalp subject's were identified by cloning and sequencing of the conserved ribosomal unit regions (16s for bacterial and 28s-its for fungal) and were further quantified by quantitative pcr. the two main bacterial species found on the scalp surface were propionibacterium acnes and staphylococcus epider ...201323483996
quantitative analysis of the cutaneous malassezia microbiota in 770 healthy japanese by age and gender using a real-time pcr assay.although the lipophilic yeasts of the genus malassezia are part of the cutaneous microbiota in healthy individuals, they are also associated with several skin diseases, such as seborrheic dermatitis. however, the effects of age and gender on the malassezia microbiota have not been completely elucidated. we analyzed the cutaneous malassezia microbiota of 770 healthy japanese using the highly accurate real-time pcr with a taqman probe to investigate the effects of age and gender on the malassezia ...201019462267
β-endorphin enhances the phospholipase activity of the dandruff causing fungi malassezia globosa and malassezia restricta.β-endorphin is known to stimulate phospholipase production by malassezia pachydermatis during canine dermatoses. the role of β-endorphin in malassezia infection in humans is not well studied. the present study compares the influence of β-endorphin on malassezia globosa and malassezia restricta isolated from patients with seborrhoeic dermatitis/dandruff (sd/d) and healthy controls. malassezia isolates (five each of the two species from patients and healthy controls) were grown on modified dixon's ...201727497434
evaluation of malassezia and common fungal pathogens in subtypes of chronic rhinosinusitis.fungal hypersensitivity and fungal microbiome dysbiosis are possible etiologies of chronic rhinosinusitis. the sinus fungal microbiome is not well characterized; novel sinus-associated fungi, including malassezia, have only recently been described. the goals for this study were to verify malassezia as a dominant component of the sinus microbiome, to speciate sinus malassezia, and to compare select fungal species in chronic rhinosinusitis (crs) subtypes with known fungal association to chronic rh ...201627153455
malassezia arunalokei sp. nov., a novel yeast species isolated from seborrheic dermatitis patients and healthy individuals from india.the majority of species within the genus malassezia are lipophilic yeasts that colonize the skin of warm-blooded animals. two species, malassezia globosa and malassezia restricta, are implicated in the causation of seborrheic dermatitis/dandruff (sd/d). during our survey of sd/d cases, we isolated several species of malassezia and noticed vast variations within a few lipid-dependent species. variations observed in the phenotypic characteristics (colony morphology, absence of catalase activity, g ...201627147721
in vitro anti-malassezia activity of castanea crenata shell and oil-soluble glycyrrhiza extracts.a new shampoo with anti-malassezia properties obtained from various plants is required to provide seborrheic dermatitis patients with a wider range of treatment options.201728566909
a new procedure for the cloning, expression and purification of the β-carbonic anhydrase from the pathogenic yeast malassezia globosa, an anti-dandruff drug target.malassezia yeasts are almost exclusively the single eukaryotic members of the fungal flora of the skin. malassezia globosa and malassezia restricta are found on the skin of practically all humans. malassezia globosa is highly implicated in the pathogenesis of dandruff and its genome encodes for only one carbonic anhydrases (cas, ec 4.2.1.1) belonging to the β-class (mgca). it has been indeed demonstrated that in many pathogenic microorganisms, cas are essential for their life cycle and their inh ...201626523349
identification and characterization of lipases from malassezia restricta, a causative agent of dandruff.dandruff, a skin disorder affecting 50% of the world population, is linked with proliferation of lipophilic yeasts of the genus malassezia (particularly malassezia globosa and m. restricta). most malassezia species show a unique lipid dependency and require external lipids for growth. genome mining of the incomplete m. restricta genome led to the identification of eight lipase sequences. sequences representing the class 3 and lip lipase families were used to clone the lipases mrlip1, mrlip2 and ...201526298017
genotype analyses of human commensal scalp fungi, malassezia globosa, and malassezia restricta on the scalps of patients with dandruff and healthy subjects.dandruff and seborrheic dermatitis are common afflictions of the human scalp caused by commensal scalp fungi belonging to the genus malassezia. malassezia globosa and malassezia restricta are the predominant species found on the scalp. the intergenic spacer regions of these species' rrna genes contain short sequence repeats (ssr): (gt)n and (ct)n in m. globosa and (ct)n and (at)n in m. restricta. in the present study, we compared the genotypes (ssr) of m. globosa and m. restricta colonizing the ...201424792417
molecular epidemiology of malassezia globosa and malassezia restricta in sudanese patients with pityriasis versicolor.pityriasis versicolor is a superficial infection of the stratum corneum caused by malassezia yeasts. the cutaneous malassezia globosa and malassezia restricta in sudanese patients with pityriasis versicolor were elucidated using a molecular-based, culture-independent method and compared with that in healthy individuals. scale samples were collected by applying an opsite™ transparent dressing to lesional and non-lesional sites on 29 sudanese patients with pityriasis versicolor and 54 healthy indi ...201323054329
molecular identification of malassezia species isolated from dermatitis affections.the lipophilic yeast of the genus malassezia are opportunistic microorganisms of the skin microflora but they can be agents of various dermatomycoses. the aim of this study was to perform molecular identification of the commonly isolated malassezia species from various dermatomycoses in our region. thirty strains of malassezia were isolated from different dermatologic affections: pityriasis versicolor (17), dandruff (5), seborrheic dermatitis (4), onyxis (2), folliculitis (1) and blepharitis (1) ...200918643889
do malassezia species play a role in exacerbation of scalp psoriasis?clinical expression of psoriasis is in part dependent on external factors, such as drugs, microorganisms or stress. however convincing evidence of the role of malassezia species in the pathogenesis of the psoriasis is still lacking.201424411177
escmid and ecmm joint clinical guidelines for the diagnosis and management of rare invasive yeast infections.the mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than candida being no exception. this is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifung ...201424102785
acute appendicitis due to malassezia restricta. 201121797936
the positive benefit of lactobacillus paracasei ncc2461 st11 in healthy volunteers with moderate to severe dandruff.dandruff is a common persistent, relapsing inflammatory condition affecting the scalp. an imbalanced proportion of the major bacterial and fungal populations colonising the scalp, a skin barrier dysfunction, and hyperseborrhoea are three main etiological factors of dandruff. the efficacy of lactobacillus paracasei ncc 2461 st11 (st11) to manage dandruff and to restore a balanced scalp microbiome was assessed. sixty healthy male volunteers aged 18 to 60 years with moderate to severe dandruff cons ...201728789559
in vitro antifungal activity of topical and systemic antifungal drugs against malassezia species.the strict nutritional requirements of malassezia species make it difficult to test the antifungal susceptibility. treatments of the chronic and recurrent infections associated with malassezia spp. are usually ineffective. the objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of malassezia species against 16 antifungal drugs used for topical or systemic treatment. isolates were identified by restriction fragment length polymorphism. minimal inhibitory ...201323496653
chemical organization of the cell wall polysaccharide core of malassezia restricta.malassezia species are ubiquitous residents of human skin and are associated with several diseases such as seborrheic dermatitis, tinea versicolor, folliculitis, atopic dermatitis, and scalp conditions such as dandruff. host-malassezia interactions and mechanisms to evade local immune responses remain largely unknown. malassezia restricta is one of the most predominant yeasts of the healthy human skin, its cell wall has been investigated in this paper. polysaccharides in the m. restricta cell wa ...201424627479
antimycotics suppress the malassezia extract-induced production of cxc chemokine ligand 10 in human keratinocytes.malassezia, a lipophilic yeast, exacerbates atopic dermatitis. malassezia products can penetrate the disintegrated stratum corneum and encounter subcorneal keratinocytes in the skin of atopic dermatitis patients. type 1 helper t (th1) cells infiltrate chronic lesions with atopic dermatitis, and antimycotic agents improve its symptoms. we aimed to identify malassezia-induced chemokines in keratinocytes and examine whether antimycotics suppressed this induction. normal human keratinocytes were inc ...201424471457
utilization of matrix-assisted laser desorption and ionization time-of-flight mass spectrometry for identification of infantile seborrheic dermatitis-causing malassezia and incidence of culture-based cutaneous malassezia microbiota of 1-month-old infants.matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (maldi-tof-ms) has been utilized for identification of various microorganisms. malassezia species, including malassezia restricta, which is associated with seborrheic dermatitis, has been difficult to identify by traditional means. this study was performed to develop a system for identification of malassezia species with maldi-tof-ms and to investigate the incidence and variety of cutaneous malassezia microbiota of ...201424387229
evaluation of expression of lipases and phospholipases of malassezia restricta in patients with seborrheic dermatitis.malassezia species (spp.) are cutaneous opportunistic pathogens and associated with various dermatological diseases including seborrheic dermatitis, dandruff and atopic dermatitis. almost all malassezia spp. are obligatorily lipid-dependent, which might be caused by lack of the myristic acid synthesis. recent genome analysis of m. restricta and m. globosa suggested that the absence of a gene encoding fatty acid synthesis might be compensated by abundant genes encoding hydrolases, which produce f ...201324003273
comprehensive analysis of the fungal microbiota of the human external auditory canal using pyrosequencing.we previously reported that the malassezia microbiota of the external auditory canal were composed principally of malassezia slooffiae and malassezia restricta. in the present study, the fungal microbiota of the external auditory canal were comprehensively analyzed by pyrosequencing on an flx-454 platform. of 17 samples tested, the fungal microbiota of 14 samples were composed of > 90% malassezia species; either m. slooffiae or m. restricta was abundant in individual samples. the external audito ...201728250358
efficacy and safety of cream containing climbazole/piroctone olamine for facial seborrheic dermatitis: a single-center, open-label split-face clinical study.seborrheic dermatitis (sd) is a multifactorial disease; malassezia species play an important role in its pathogenesis.201627904273
domestic shower hose biofilms contain fungal species capable of causing opportunistic infection.the domestic environment can be a source of pathogenic bacteria. we show here that domestic shower hoses may harbour potentially pathogenic bacteria and fungi. well-developed biofilms were physically removed from the internal surface of shower hoses collected in four locations in england and scotland. amplicon pyrosequencing of 16s and 18s rrna targets revealed the presence of common aquatic and environmental bacteria, including members of the actinobacteria, alphaproteobacteria, bacteroidetes a ...201627740540
the assessment of epiphytic yeast diversity in sugarcane phyllosphere in thailand by culture-independent method.the diversity of epiphytic yeasts from sugarcane (saccharum officinarum linn.) phyllospheres in thailand was investigated by culture-independent method based on the analysis of the d1/d2 domains of the large subunit rrna gene sequences. forty-five samples of sugarcane leaf were collected randomly from ten provinces in thailand. a total of 1342 clones were obtained from 45 clone libraries. 426 clones (31.7 %) were closely related to yeast strains in the genbank database, and they were clustered i ...201526615738
characterisation and expression analysis of mrlip1, a class 3 family lipase of malassezia restricta.the genus malassezia is associated with a wide range of skin diseases and is the predominant fungal genus isolated from human skin. of the 14 malassezia species identified, m. restricta is the most abundant fungal species found from both healthy and diseased skin. emerging evidences have suggested that extracellular lipases of malassezia play a critical role in its survival on the host skin surface. this study aimed to characterise the lipase 1 homologue (mrlip1) in m. restricta and to analyse i ...201526404462
characterization of the major bacterial-fungal populations colonizing dandruff scalps in shanghai, china, shows microbial disequilibrium.dandruff is a scalp disorder characterized by the formation of flaky white-yellowish scales due to an altered proliferation and differentiation status; a disrupted barrier function; a decrease in the level of hydration and of natural moisturizing factors (nmf) in the scalp, with a persistent and relapsing inflammatory condition. it was recently reported that an imbalance between bacterial and fungal species colonizing the scalp of french volunteers was associated with dandruff condition. the pur ...201525739873
prolonged fever and splenic lesions caused by malassezia restricta in an immunocompromised patient.malassezia species are commonly found on human skin as commensals but can cause invasive infections in premature infants and immunocompromised hosts. due to their fastidious growth, diagnosis of malassezia infections can prove challenging. molecular techniques can aid in diagnosis and treatment of invasive infections. we describe the case of a pediatric oncology patient with splenic lesions secondary to malassezia restricta.201425187171
malassezia species in healthy skin and in dermatological conditions.the genus malassezia comprises lipophilic species, the natural habitat of which is the skin of humans and other warm-blooded animals. however, these species have been associated with a diversity of dermatological disorders and even systemic infections. pityriasis versicolor is the only cutaneous disease etiologically connected to malassezia yeasts. in the other dermatoses, such as malassezia folliculitis, seborrheic dermatitis, atopic dermatitis, and psoriasis, these yeasts have been suggested t ...201626710919
detection and identification of malassezia species in domestic animals and aquatic birds by pcr-rflp.the present study aimed at detection and species-level identification of the malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds and 5 turkeys. tape-strip samples were examined by direct microscopy. all samples were inoculated on mod ...201527175148
epidemiological characteristics of malassezia folliculitis and use of the may-grünwald-giemsa stain to diagnose the infection.various bacterial, fungal, parasitic, and viral pathogens can cause folliculitis, which is often mistakenly treated with antibiotics for months or even years. a laboratory diagnosis is required before therapy can be planned. here, we describe the prevalence and risk factors, as well as the clinical, cytological, and mycological characteristics, of patients with malassezia folliculitis (mf) in adana, turkey. we also report the treatment responses of the mf patients and describe the malassezia spp ...201323706503
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