| isolation of malassezia sympodialis and malassezia globosa from healthy pet cats. | | 1997 | 9292976 |
| characterization of malassezia species by means of phenotypic characteristics and detection of electrophoretic karyotypes by pulsed-field gel electrophoresis (pfge). | a total of 220 isolates of the genus malassezia obtained from affected skin of animals and humans were included in this study. the isolates were examined for their belonging to the known species malassezia pachydermatis, malassezia sympodialis, malassezia furfur, malassezia obtusa, malassezia restricta, malassezia globosa and malassezia slooffiae by means of phenotypic and biochemical characteristics as well as their ability to assimilate polyethylen(20)-sorbitan-esters (tween 20, 40, 60, 80). t ... | 1999 | 10536433 |
| isolation and characterization of a major antigenic component of malassezia globosa to ige antibodies in sera of patients with atopic dermatitis. | three major components of malassezia globosa were isolated from 2-me extracts of this fungus by ion-exchange column chromatography and are referred to as malg46a, malg46b and malg67, respectively. ige antibodies to these components in the sera of patients with ad were detected by immunoblots. in western blot, ige antibodies to malg46b were most frequently detected in the sera of ad patients. dot blot with the malg46b-containing fraction immunologically reacted with 69% of the sera of the patient ... | 2000 | 10888355 |
| malassezia globosa as the causative agent of pityriasis versicolor. | the taxonomic revision carried out in 1996 on the genus malassezia, which now comprises seven different species, made necessary a re-evaluation of the data concerning the ecology and pathogenicity of these lipophilic yeasts. very little has been published since then. | 2000 | 11069459 |
| identification of malassezia species isolated from patients with seborrhoeic dermatitis, atopic dermatitis, pityriasis versicolor and normal subjects. | we identified malassezia species isolated from 42 patients with seborrhoeic dermatitis, 17 patients with atopic dermatitis, 22 patients with pityriasis versicolor, 35 normal subjects and 73 healthy medical students. regarding the prevalence of malassezia species in the 35 normal subjects, the frequency of isolation of malassezia globosa was 22%, m. sympodialis 10% and m. furfur 3%. m. slooffiae, m. pachydermatis, m. restricta and m. obtusa were infrequently isolated from normal skin. two differe ... | 2000 | 11092380 |
| molecular analysis of malassezia microflora on the skin of atopic dermatitis patients and healthy subjects. | members of the genus malassezia, lipophilic yeasts, are considered to be one of the exacerbating factors in atopic dermatitis (ad). we examined variation in cutaneous colonization by malassezia species in ad patients and compared it with variation in healthy subjects. samples were collected by applying transparent dressings to the skin lesions of ad patients. dna was extracted directly from the dressings and amplified in a specific nested pcr assay. malassezia-specific dna was detected in all sa ... | 2001 | 11574560 |
| skin colonization by malassezia species in neonates: a prospective study and relationship with neonatal cephalic pustulosis. | to assess skin colonization by malassezia species in full-term healthy newborns, to investigate factors associated with colonization, and to look at acnelike cephalic pustulosis associated with this carriage. | 2002 | 11843642 |
| malassezia species in skin diseases. | since the taxonomic revision carried out in 1996, enlarging the genus malassezia to comprise seven different species, a number of studies have investigated from different points of view -- mycological, molecular and immunological -- the relationships of these species with the pathologies associated with lipophilic yeasts, as well as its presence in healthy skin. from these studies, it now appears clear that malassezia globosa is the main species associated with pityriasis versicolor, which is th ... | 2002 | 11964913 |
| isolation of malassezia globosa and m. sympodialis from patients with pityriasis versicolor in spain. | pityriasis versicolor is a superficial infection of the stratum corneum by several yeast species formerly collectively named malasseziafurfur. the genus malassezia has been recently enlarged with new species. with the exception of m. pachydermatis, the remaining six species have an absolute requirement in vitro for supplementation of long-chain fatty acids in media. these lipophilic yeasts comprise six species: m. furfur, m. globosa, m. obtusa, m. restricta, m. slooffiae and m. sympodialis. the ... | 2002 | 12171443 |
| sequence diversity of the intergenic spacer region of the rrna gene of malassezia globosa colonizing the skin of patients with atopic dermatitis and healthy individuals. | the lipophilic yeast malassezia globosa is one of the major constituents of the mycoflora of the skin of patients with atopic dermatitis (ad). we compared the genotypes of m. globosa colonizing the skin surface of 32 ad patients and 20 healthy individuals for polymorphism of the intergenic spacer (igs) 1 region of the rrna gene. sequence analysis demonstrated that m. globosa was divided into four major groups, which corresponded to the sources of the samples, on the phylogenetic tree. of the fou ... | 2003 | 12843037 |
| [first case of malassezia globosa isolation in serbia]. | today is known that genus malassezia includes seven species: m. furfur, m. sympodialis, m. obtusa, m. globosa, m. restricta, m. sloofflae and m. pachydermatis, but role of each of the species in the pathogenesis of disease has not been elucidated yet, so further laboratory isolation and identification are necessary. we report the first case of isolation of malassezia globosa in serbia (belgrade), in a patient suffering from pityriasis versicolor. identification of m. globosa was based on macrosc ... | 2003 | 15114787 |
| superficial fungal infections. | superficial fungal infections arise from a pathogen that is restricted to the stratum corneum, with little or no tissue reaction. in this seminar, three types of infection will be covered: tinea versicolor, piedra, and tinea nigra. tinea versicolor is common worldwide and is caused by malassezia spp, which are human saprophytes that sometimes switch from yeast to pathogenic mycelial form. malassezia furfur, malassezia globosa, and malassezia sympodialis are most closely linked to tinea versicolo ... | 2004 | 15451228 |
| [taxonomy of malassezia furfur: state of the art]. | malassezia furfur is a lipophilic yeast considered as a normal component of the human skin flora. apart from pityriasis versicolor, m. furfur has been linked to several skin diseases such as seborrheic dermatitis, folliculitis or atopic dermatitis. moreover, these yeasts have been reported as agent of invasive human diseases including pneumonia, catheter-associated sepsis and peritonitis. the existence of morphological, serological, metabolical, biochemical and karyotipical differences has been ... | 1997 | 15538815 |
| isolation of malassezia species from healthy cats and cats with otitis. | lipid-dependent malassezia species have recently been cultured from veterinary specimens. the identification of malassezia species isolates from animals is important to clarify the epidemiology of these lipophilic yeasts. malassezia species were cultured from the external ear canals of 63 out of 99 cats with otitis and 12 of 52 (23%) healthy control cats. the rate of isolation in affected animals versus controls was highly significant (p<0.01). malassezia pachydermatis was isolated as a pure cul ... | 2005 | 15922220 |
| antifungal activities of tacrolimus and azole agents against the eleven currently accepted malassezia species. | the lipophilic yeast malassezia is an exacerbating factor in atopic dermatitis (ad) and colonizes the skin surface of patients with ad. with the goal of reducing the number of malassezia cells, we investigated the antifungal activities of a therapeutic agent for ad, tacrolimus, and the azole agents itraconazole and ketoconazole against malassezia species in vitro. we examined 125 strains of the 11 currently accepted malassezia species by using the agar dilution method. all strains of the 11 mala ... | 2005 | 15956404 |
| identification of malassezia species from tunisian patients with pityriasis versicolor and normal subjects. | the genus malassezia (pityrosporum), recognized as a member of microbiological flora of the skin in humans and warm-blooded animals, has been recently revised to include 10 malassezia species. the aim of the study was the isolation, identification and analysis of malassezia species distribution in skin of healthy volunteers and lesions of pityriasis versicolor (pv). | 2005 | 15982205 |
| malassezia yeast species isolated from patients with dermatologic lesions. | yeasts of the genus malassezia form a normal component of skin flora, but are also associated with several dermatological disorders. since 1996, the description of new species in this genus have led to new questions about their epidemiology and pathogenicity. | 2005 | 16022373 |
| malassezia and candida colonisation on glans penis of circumcised men. | the malassezia yeast are members of the normal human cutaneous flora in adults. they also are reported as part of the microflora of the male genital region in mostly uncircumcised males. it has been reported that malassezia sympodialis and malassezia globosa are the most frequent yeast belonging to the resident microflora of the penis as in other human skin areas. the aim was to evaluate the prevalence of malassezia and candida yeast colonisation on the glans penis of circumcised males. impressi ... | 2005 | 16115108 |
| [species differentiation of yeasts of the genus malassezia with fourier transform infrared spectroscopy]. | 83 malassezia strains (65 wild isolates and 18 reference strains) were differentiated to the species level using conventional methods including morphological and biochemical features. these strains were further analyzed by fourier transform infrared spectroscopy (ft-irs). | 2006 | 16170542 |
| activity of the triazole antifungal r126638 as assessed by corneofungimetry. | r126638 is a novel triazole exhibiting potent in vitro and in vivo antifungal activity against fungal pathogens including dermatophytes and yeasts. | 2006 | 16247249 |
| a case of pityriasis versicolor atrophicans. | a case of pityriasis versicolor atrophicans caused by malassezia globosa is reported in a 49-year-old woman. diagnosis was based on direct microscopic examination and culture. differential diagnosis was performed with respect to other skin diseases manifesting with cutaneous atrophy. | 2005 | 16262883 |
| malassezia yeasts and pityriasis versicolor. | to analyze the relationships among different malassezia species and pityriasis versicolor, the only human disease in which the etiologic role of these fungi is fully accepted (although the species implicated remains a matter of discussion). | 2006 | 16514338 |
| effect of circumcision on genital colonization of malassezia spp. in a pediatric population. | this study aimed to determine the prevalence of yeast fungi in samples from the glans penis and prepuce of circumcised and uncircumcised children. impression preparations were made on modified dixon and leeming-notman agar without cycloheximide. the isolates were identified by morphological and physiological characteristics. the yeasts were detected in 32 (28.6%) of 112 children, 12 (18.2%) being among 66 circumcised and 20 (43.5%) among 46 uncircumcised children. the most common species was mal ... | 2006 | 16519013 |
| distribution of malassezia species in pityriasis versicolor and seborrhoeic dermatitis in greece. typing of the major pityriasis versicolor isolate m. globosa. | the expansion of the genus malassezia has generated interest in the epidemiological investigation of the distribution of new species in a range of dermatoses, on which variable results have been reported from different geographical regions. no data are thus far available from south-east europe (greece). | 2006 | 16634886 |
| in vitro activity of r126638 and ketoconazole against malassezia species. | the in vitro activity of a new triazole r126638 against malassezia yeasts was compared with that of ketoconazole. with the agar dilution technique, minimal inhibitory concentrations were lower for r126638 compared with ketoconazole against malassezia globosa, m. obtusa, m. slooffiae, m. restricta and two strains of m. sympodialis. on human stratum corneum in vitro, both r126638 and ketoconazole were very effective in reducing the production of hyphae from 15% to 1% with r126638 and to 2% with ke ... | 2006 | 16874415 |
| a modified christensen's urea and clsi broth microdilution method for testing susceptibilities of six malassezia species to voriconazole, itraconazole, and ketoconazole. | two supplemented broths (christensen's urea with 0.1% tween 80 and 0.5% tween 40 and rpmi 1640 with 1% glycerol, 1% peptone, 1.8% glucose, and 0.05% tween 80) were evaluated to determine voriconazole, itraconazole, and ketoconazole mics for 200 malassezia sp. isolates. malassezia globosa and m. restricta were the least susceptible species (mics at which 90% of the isolates tested were inhibited, 1 to >or=8 microg/ml versus 0.25 to 1 microg/ml). | 2006 | 16954293 |
| cytokine secretion profile of human keratinocytes exposed to malassezia yeasts. | the lipophilic yeast malassezia is an exacerbating factor in atopic dermatitis (ad). among organisms of the malassezia species, malassezia globosa and malassezia restricta are particularly dominant on the skin of ad patients. however, the precise role of malassezia yeasts in the pathophysiology of ad remains uncertain. keratinocytes play a critical role in cutaneous inflammatory and immune responses by secreting cytokines. in this study, we attempted to determine the cytokine secretion profiles ... | 2006 | 17069617 |
| in vitro antifungal efficacy of ciclopirox olamine alone and associated with zinc pyrithione compared to ketoconazole against malassezia globosa and malassezia restricta reference strains. | the aim of this study was to determine the in vitro fungicidal and growth inhibitory activity of ciclopirox olamine alone (1% and 1.5%) or in association with 1% zinc pyrithione compared to 2% ketoconazole, against malassezia species particularly involved in the pathogenesis of seborrheic dermatitis. experiments were performed on malassezia globosa ip 2387.96 and m. restricta ip 2392.96 strains. growth inhibitory activity of the active compounds in solution was evaluated by measuring minimal inh ... | 2006 | 17146583 |
| [identification of malassezia species in patients with pityriasis versicolor in goiânia-go]. | the objectives of this study were to investigate the frequency of pityriasis versicolor occurrence and to identify yeasts of the genus malassezia in patients at the mycology laboratory of the federal university of goiás, in goiânia, state of goiás. ninety-five cases of pityriasis versicolor were diagnosed, and four species of malassezia were identified: malassezia furfur, malassezia sympodialis, malassezia globosa and malassezia obtusa. | 2006 | 17308710 |
| isolation and expression of a malassezia globosa lipase gene, lip1. | dandruff and seborrheic dermatitis (d/sd) are common hyperproliferative scalp disorders with a similar etiology. both result, in part, from metabolic activity of malassezia globosa and malassezia restricta, commensal basidiomycete yeasts commonly found on human scalps. current hypotheses about the mechanism of d/sd include malassezia-induced fatty acid metabolism, particularly lipase-mediated breakdown of sebaceous lipids and release of irritating free fatty acids. we report that lipase activity ... | 2007 | 17460728 |
| antifungal and antioxidant activities of the phytomedicine pipsissewa, chimaphila umbellata. | bioassay-guided fractionation of chimaphila umbellata (l.) w. bart (pyrolaceae) ethanol extracts led to the identification of 2,7-dimethyl-1,4-naphthoquinone (chimaphilin) as the principal antifungal component. the structure of chimaphilin was confirmed by 1h and 13c nmr spectroscopy. the antifungal activity of chimaphilin was evaluated using the microdilution method with saccharomyces cerevisiae (0.05mg/ml) and the dandruff-associated fungi malassezia globosa (0.39mg/ml) and malassezia restrict ... | 2008 | 17950387 |
| dandruff-associated malassezia genomes reveal convergent and divergent virulence traits shared with plant and human fungal pathogens. | fungi in the genus malassezia are ubiquitous skin residents of humans and other warm-blooded animals. malassezia are involved in disorders including dandruff and seborrheic dermatitis, which together affect >50% of humans. despite the importance of malassezia in common skin diseases, remarkably little is known at the molecular level. we describe the genome, secretory proteome, and expression of selected genes of malassezia globosa. further, we report a comparative survey of the genome and secret ... | 2007 | 18000048 |
| malassezia globosa and restricta: breakthrough understanding of the etiology and treatment of dandruff and seborrheic dermatitis through whole-genome analysis. | dandruff and seborrheic dermatitis (d/sd) share an etiology dependent upon three factors: sebum, microbial metabolism (specifically, malassezia yeasts), and individual susceptibility. advances in microbiological and analytical techniques permit a more detailed understanding of these etiologic factors, especially the role of malassezia. malassezia are lipid-dependent and demonstrate adaptation allowing them to exploit a narrow niche on sebum-rich skin. work in our and our collaborators' laborator ... | 2007 | 18004291 |
| [concordance between phenotypical features and pcr-rea for the identification of malassezia spp]. | the genus malassezia has been recently revised and nowadays includes 11 species that cannot always be differentiated from each other by physiological and morphological tests. this study was aimed to evaluate the correlation between a molecular method and conventional phenotypic features in the identification of malassezia spp. to achieve this aim, 92 argentinean clinical strains isolated between 2001 and 2005 were analyzed along with three reference strains (malassezia furfur cbs 7019, malassezi ... | 2007 | 18095760 |
| analysis of malassezia microbiota in healthy superficial human skin and in psoriatic lesions by multiplex real-time pcr. | yeasts from the genus malassezia are members of the normal biota of human skin, and may play a role in dermatopathology. our previous study of the fungal microbiota from healthy subjects and from patients with psoriasis using clone library analysis revealed the presence of five malassezia species and four uncharacterized phylotypes. we now compared the malassezia microbiota from six healthy body locations and two psoriatic lesions, and evaluated its stability over time using multiplex real-time ... | 2008 | 18294199 |
| [isolation and identification of malassezia spp. in pytiriasis versicolor, seborrheic dermatitis and healthy skin.]. | the taxonomy of the genus malassezia has been recently revised and enlarged to include seven distinct species. the aim of the study was to analyse the prevalence of these species in the cutaneous lesions of pityriasis versicolor and dermatitis seborrhoeica, as well as in the normal skin. seventy-five patients with pityriasis versicolor, 75 of dermatitis seborrhoeica, and 150 samples of normal skin (75 from the forehead and 75 from the shoulders) were studied. a direct microscopy with koh + parke ... | 1999 | 18473598 |
| distribution of malassezia species in patients with psoriasis and healthy individuals in tehran, iran. | psoriasis is a non-contagious disorder that affects the skin as red scaly patches. although the role of malassezia species in the pathogenesis of psoriasis is still not fully understood, it is thought that these lipophilic yeasts might be a trigger factor in the exacerbation of psoriatic lesions. | 2008 | 18616765 |
| molecular identification of malassezia species isolated from dermatitis affections. | the lipophilic yeast of the genus malassezia are opportunistic microorganisms of the skin microflora but they can be agents of various dermatomycoses. the aim of this study was to perform molecular identification of the commonly isolated malassezia species from various dermatomycoses in our region. thirty strains of malassezia were isolated from different dermatologic affections: pityriasis versicolor (17), dandruff (5), seborrheic dermatitis (4), onyxis (2), folliculitis (1) and blepharitis (1) ... | 2009 | 18643889 |
| in vitro modulation of human keratinocyte pro- and anti-inflammatory cytokine production by the capsule of malassezia species. | malassezia spp. are commensal, cutaneous fungi that are implicated in seborrhoeic dermatitis. we hypothesize that the lipid-rich capsule of malassezia spp. masks the organism from host detection, and depletion of this layer elicits an inflammatory response. to test this, preparations of capsulated or acapsular [10% (v/v) triton x-100 treated], viable and nonviable, exponential or stationary phase malassezia furfur, malassezia globosa, malassezia obtusa, malassezia restricta, malassezia slooffiae ... | 2008 | 18752620 |
| pramiconazole, a triazole compound for the treatment of fungal infections. | pramiconazole from barrier therapeutics inc is a new addition to the family of triazole antifungal agents that act by inhibiting fungal cell membrane ergosterol synthesis, thereby leading to increased cell permeability and destruction. barrier therapeutics was developing an oral formulation of pramiconazole for the potential treatment of seborrheic dermatitis (erythematosquamous skin disease), onychomycosis and dermatomycosis (including tinea versicolor, tinea pedis and tinea cruris/corporis). i ... | 2008 | 18763217 |
| [pityriasis versicolor and the yeasts of genus malassezia]. | although pityriasis versicolor is the only human disease for which malassezia yeasts have been fully established as pathogens, it is still not clear which species are implicated. most studies carried out in recent years support our hypothesis - proposed in 1999 - that malassezia globosa is the predominant species in pityriasis versicolor lesions, at least in temperate climates. confirmation of this hypothesis could help us understand the conditions, as yet unclear, that induce transformation of ... | 2008 | 19091214 |
| the range of molecular methods for typing malassezia. | the recent sequencing of the whole genome of malassezia globosa and m. restricta forms the basis for molecular epidemiology studies and instigates investigations into their respective virulence factors. thus, reviewing current knowledge on malassezia molecular typing methods would reveal the pros and cons of each method and would highlight potential scarcity of epidemiological data regarding this ubiquitous fungal commensal and pathogen. | 2009 | 19283910 |
| distribution of malassezia species in seborrhoeic dermatitis: correlation with patients' cellular immune status. | malassezia species are implicated in the pathogenesis of seborrhoeic dermatitis (sd), but the relationship between each species and the disorder remains unclear. it is hypothesised that the pathogenesis of sd has an immune component, which is supported by the increased incidence in patients with immunosuppressive disorders. the purpose of our study was to analyse the prevalence of malassezia species in lesional skin of sd, and to assess the distribution of the species according to severity of th ... | 2010 | 19486300 |
| identification of the major allergen of malassezia globosa relevant for atopic dermatitis. | malasseziaglobosa constitutes a part of the normal flora of human skin, but may induce ige production in atopic dermatitis (ad). however, information on m.globosa allergens is scant. | 2009 | 19540092 |
| epidemiology of pityriasis versicolor in adana, turkey. | pityriasis versicolor is a common superficial mycoses of the skin. it is now recognized that the causative organisms of this infection are different species of malassezia. the aim of this study was to determine the distribution of malassezia species in patients with pityriasis versicolor in adana, turkey. in total, 97 patients positive for malassezia elements, namely, yeast cells and short hyphae in microscopic examination, were included in the study. all samples were inoculated in plates contai ... | 2009 | 19583685 |
| fungal diversity in composting process of pig manure and mushroom cultural waste based on partial sequence of large subunit rrna. | fungal diversity during composting was investigated by culture-independent rdna sequence analysis. composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (hazaka system), and samples were collected at various stages. based on partial sequence analysis of large subunit (lsu) ribosomal rna (rrna) and sequence identity values, a total of 12 different fungal species were found at six sampling sites; geotrichum sp., debaryomyces hansenii, monographella ni ... | 2009 | 19734710 |
| identification of malassezia species isolated from iranian seborrhoeic dermatitis patients. | in recent years, the genus malassezia has come to be considered important in the etiology of seborrhoeic dermatitis (sed). the aim of present study was identification of malassezia species on the lesions of iranian sed patients. | 2010 | 20184091 |
| differentially expressed genes associated with tryptophan-dependent pigment synthesis in malassezia furfur- a comparison with the recently published genome of malassezia globosa. | summary malassezia (m.) furfur, a commensal organism found on the human skin, produces a wide range of pigments and fluorochromes when cultured with tryptophan as a sole nitrogen source. some compounds of this pigment metabolism may provide an explanation for clinical characteristics of pityriasis versicolor (pv), a frequent skin disease in humans characterised by long-lasting pigmentary changes. malassezia globosa is currently regarded as the causative agent of pv, but tryptophan-dependent pigm ... | 2010 | 20202114 |
| prevalence of malassezia species in pityriasis versicolor lesions in northeast argentina. | malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens. | 2010 | 20346299 |
| identification of malassezia species from tunisian patients with pityriasis versicolor. | pityriasis versicolor is caused by malassezia sp. it is a common worldwide mycosis. recently, eleven species are known of the malassezia genus, and are identified in vitro by their morphological characteristics, biochemical tests and by molecular biology. the aim of this study is the identification of malassezia species from tunisian patients with pityriasis versicolor. | 2010 | 20415164 |
| [pityriasis versicolor: isolation and identification of the main species of malassezia]. | species of the genus malassezia isolated were: malassezia sympodialis (16.66%), malassezia furfur (12.50%), malassezia globosa (11.45%), and malassezia slooffiae (2.10%). malassezia sympodialis predominated in the study. the species of malassezia identified did not show correlation with clinical variants and with the distribution of pityriasis versicolor lesions in relation to areas of the body. | 2010 | 20464101 |
| cutaneous malasseziasis: four case reports of atypical dermatitis and onychomycosis caused by malassezia. | this study investigates four special cases associated with malassezia. | 2010 | 20465637 |
| insights into the phylogeny or arylamine n-acetyltransferases in fungi. | previous studies have shown that eumycetes fungi can acylate arylamine thanks to arylamine n-acetyltransferases, xenobiotic-metabolizing enzymes also found in animals and bacteria. in this article, we present the results of mining 96 available fungal genome sequences for arylamine n-acetyltransferase genes and propose their phylogeny. the filamentous pezizomycotina are shown to possess many putative n-acetyltransferases, whilst these are often lacking in other fungal groups. the evolution of the ... | 2010 | 20676885 |
| the human ku autoantigen shares amino acid sequence homology with fungal, but not bacterial and viral, proteins. | context: molecular mimicry between autoantigens and microbial antigens is a possible triggering mechanism of autoimmunity. human ku is a dna-associated autoantigen targeted by autoantibodies in patients with systemic lupus erythematosus and related disorders; available data are consistent with a role of molecular mimicry in the pathogenesis of anti-ku autoimmunity, but no research exist on this topic. objective: we aimed to define the most probable microbial triggers of anti-ku autoimmunity via ... | 2011 | 20718578 |
| functional expression and characterization of cyp51 from dandruff-causing malassezia globosa. | malassezia globosa is one of the most common yeasts to cause various human skin diseases including dandruff and seborrheic dermatitis. genomic analysis of m. globosa revealed four putative cytochrome p450 (cyp) enzymes. here, we report the purification and characterization of recombinant cyp51, a putative lanosterol 14α-demethylase, from m. globosa. the m. globosa cyp51 was expressed heterologously in escherichia coli, followed by purification. purified cyp51 showed a typical reduced co-differen ... | 2010 | 21114623 |
| extracellular phospholipase activity of malassezia spp. isolated from individuals with and without dermatological disease. | background: the malassezia genus includes mainly lipophilic yeasts belonging to the cutaneous microbiota of man and other mammals. some malassezia species have been associated with various dermatological diseases. the factors permitting the transformation of yeasts of the malassezia genus from a commensal organism to a pathogenic agent are still little known but the production of various enzymes such as lipase, phospholipase and lipoxygenase could contribute to the pathogenic activity of these y ... | 2011 | 21645629 |
| zinc pyrithione inhibits yeast growth through copper influx and inactivation of iron-sulfur proteins. | zinc pyrithione (zpt) is an antimicrobial material with widespread use in antidandruff shampoos and antifouling paints. despite decades of commercial use, there is little understanding of its antimicrobial mechanism of action. we used a combination of genome-wide approaches (yeast deletion mutants and microarrays) and traditional methods (gene constructs and atomic emission) to characterize the activity of zpt against a model yeast, saccharomyces cerevisiae. zpt acts through an increase in cellu ... | 2011 | 21947398 |
| identification of malassezia yeast species isolated from patients with pityriasis versicolor. | pityriasis versicolor (pv) is a disease with worldwide distribution. twelve different species of malassezia yeast have been described. the objective of this study was to determine which species of malassezia are more prevalent in patients with pityriasis versicolor. samples were collected by scraping the lesions of 87 patients with a clinical suspicion of pityriasis versicolor. the samples were then submitted to fungal microscopy and culture to identify the species. the species found were: malas ... | 2011 | 21987156 |
| Purification and characterization of a secretory lipolytic enzyme, MgLIP2, from Malassezia globosa. | Malassezia globosa is a lipid-dependent yeast that is found on the human skin and is associated with various skin disorders, including dandruff and seborrhoeic dermatitis (SD). Despite its important role in skin diseases, the molecular basis for its pathogenicity is poorly understood. The current hypothesis is that dandruff and SD are linked to fatty acid metabolism and secretory lipolytic enzymes, which hydrolyse sebaceous lipids and release irritating free fatty acids. A previous genomic analy ... | 2011 | 22016565 |
| residue asn277 affects the stability and substrate specificity of the smg1 lipase from malassezia globosa. | thermostability and substrate specificity are important characteristics of enzymes for industrial application, which can be improved by protein engineering. smg1 lipase from malassezia globosa is a mono- and diacylglycerol lipase (mdl) that shows activity toward mono- and diacylglycerols, but no activity toward triacylglycerols. smg1 lipase is considered a potential biocatalyst applied in oil/fat modification and its crystal structure revealed that an interesting residue-asn277 may contribute to ... | 2015 | 25837472 |
| urban transit system microbial communities differ by surface type and interaction with humans and the environment. | public transit systems are ideal for studying the urban microbiome and interindividual community transfer. in this study, we used 16s amplicon and shotgun metagenomic sequencing to profile microbial communities on multiple transit surfaces across train lines and stations in the boston metropolitan transit system. the greatest determinant of microbial community structure was the transit surface type. in contrast, little variation was observed between geographically distinct train lines and statio ... | 2016 | 27822528 |
| genomic insights into the atopic eczema-associated skin commensal yeast malassezia sympodialis. | malassezia commensal yeasts are associated with a number of skin disorders, such as atopic eczema/dermatitis and dandruff, and they also can cause systemic infections. here we describe the 7.67-mbp genome of malassezia sympodialis, a species associated with atopic eczema, and contrast its genome repertoire with that of malassezia globosa, associated with dandruff, as well as those of other closely related fungi. ninety percent of the predicted m. sympodialis protein coding genes were experimenta ... | 2013 | 23341551 |
| [microbial composition of various surfaces of skin during develop- ment of atopic dermatitis based on data from maldi-tof mass-spectrometry identification method]. | study transformation of skin microflora during development of atopic dermatitis. | 2016 | 27228668 |
| escmid and ecmm joint clinical guidelines for the diagnosis and management of rare invasive yeast infections. | the mortality associated with invasive fungal infections remains high with that involving rare yeast pathogens other than candida being no exception. this is in part due to the severe underlying conditions typically predisposing patients to these healthcare-related infections (most often severe neutropenia in patients with haematological malignancies), and in part due to the often challenging intrinsic susceptibility pattern of the pathogens that potentially leads to delayed appropriate antifung ... | 2014 | 24102785 |
| biochemical properties of a new cold-active mono- and diacylglycerol lipase from marine member janibacter sp. strain htcc2649. | mono- and di-acylglycerol lipase has been applied to industrial usage in oil modification for its special substrate selectivity. until now, the reported mono- and di-acylglycerol lipases from microorganism are limited, and there is no report on the mono- and di-acylglycerol lipase from bacteria. a predicted lipase (named maj1) from marine janibacter sp. strain htcc2649 was purified and biochemical characterized. maj1 was clustered in the family i.7 of esterase/lipase. the optimum activity of the ... | 2014 | 24927145 |
| bacterial, fungal and protozoan carbonic anhydrases as drug targets. | the carbonic anhydrases (cas, ec 4.2.1.1), a group of ubiquitously expressed metalloenzymes, are involved in numerous physiological and pathological processes, as well as in the growth and virulence of pathogens belonging to bacteria, fungi and protozoa. | 2015 | 26235676 |
| bortezomib inhibits bacterial and fungal β-carbonic anhydrases. | inhibition of the β-carbonic anhydrases (cas, ec 4.2.1.1) from pathogenic fungi (cryptococcus neoformans, candida albicans, candida glabrata, malassezia globosa) and bacteria (three isoforms from mycobacterium tuberculosis, rv3273, rv1284 and rv3588), as well from the insect drosophila melanogaster (dmeca) and the plant flaveria bidentis (fbica1) with the boronic acid peptidomimetic proteosome inhibitor bortezomib was investigated. bortezomib was a micromolar inhibitor of all these enzymes, with ... | 2016 | 27469982 |
| yeasts as important agents of onychomycosis: in vitro activity of propolis against yeasts isolated from patients with nail infection. | the purposes of this study were to determine the frequency of the yeast species obtained from patients with clinical features of onychomycosis and the in vitro antifungal susceptibility of the yeast species to propolis. | 2013 | 22954465 |
| heterologous expression and characterization of cyp61a1 from dandruff-causing malassezia globosa. | malassezia globosa is pathogenic fungus that causes skin disorders including dandruff in humans. many yeast cytochrome cyp enzymes are involved in the biosynthesis of sterols and are considered major targets of azole antifungal agents. here, we report on the expression and characterization of the mgl_0310 gene product (cyp61a1), a sterol c-22 desaturase in m. globosa. the open reading frame of the cyp61a1 gene was amplified by pcr from m. globosa cbs 7966 genomic dna and cloned into a pcw vector ... | 2015 | 26160660 |
| evaluation of recombinant mgl_1304 produced by pichia pastoris for clinical application to sweat allergy. | we previously identified mgl_1304 secreted by malassezia globosa as a sweat antigen for patients with atopic dermatitis (ad) and cholinergic urticaria (chu). however, purifying native mgl_1304 from human sweat or culture supernatant of m. globosa (sup-mgl_1304) is costly and time-consuming. moreover, recombinant mgl_1304 expressed by using escherichia coli (tf-rmgl_1304) needs a large chaperon protein and lacks the original glycosylation of yeasts. thus, we generated a recombinant mgl_1304 by pi ... | 2015 | 26117259 |
| fungal catalases: function, phylogenetic origin and structure. | most fungi have several monofunctional heme-catalases. filamentous ascomycetes (pezizomycotina) have two types of large-size subunit catalases (l1 and l2). l2-type are usually induced by different stressors and are extracellular enzymes; those from the l1-type are not inducible and accumulate in asexual spores. l2 catalases are important for growth and the start of cell differentiation, while l1 are required for spore germination. in addition, pezizomycetes have one to four small-size subunit ca ... | 2012 | 22698962 |
| malassezia species in healthy skin and in dermatological conditions. | the genus malassezia comprises lipophilic species, the natural habitat of which is the skin of humans and other warm-blooded animals. however, these species have been associated with a diversity of dermatological disorders and even systemic infections. pityriasis versicolor is the only cutaneous disease etiologically connected to malassezia yeasts. in the other dermatoses, such as malassezia folliculitis, seborrheic dermatitis, atopic dermatitis, and psoriasis, these yeasts have been suggested t ... | 2016 | 26710919 |
| detection and identification of malassezia species in domestic animals and aquatic birds by pcr-rflp. | the present study aimed at detection and species-level identification of the malassezia yeasts in domestic animals and aquatic birds by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp). samples were collected using tape strips and swabs from 471 animals including 97 horses, 102 cattle, 105 sheep, 20 camels, 60 dogs, 30 cats, 1 hamster, 1 squirrel, 50 aquatic birds and 5 turkeys. tape-strip samples were examined by direct microscopy. all samples were inoculated on mod ... | 2015 | 27175148 |
| antifungal susceptibility of malassezia furfur, malassezia sympodialis, and malassezia globosa to azole drugs and amphotericin b evaluated using a broth microdilution method. | we studied the in vitro activity of fluconazole (fcz), ketoconazole (ktz), miconazole (mcz), voriconazole (vcz), itraconazole (itz) and amphotericin b (amb) against the three major pathogenic malassezia species, m. globosa, m. sympodialis, and m. furfur. antifungal susceptibilities were determined using the broth microdilution method in accordance with clinical and laboratory standards institute reference document m27-a3. to support lipid-dependent yeast development, glucose, peptone, ox bile, m ... | 2014 | 24965946 |
| identification and speciation of malassezia in patients clinically suspected of having pityriasis versicolor. | malassezia is a lipid-dependent yeast known to cause pityriasis versicolor, a chronic, recurrent superficial infection of skin and present as hypopigmented or hyperpigmented lesions on areas of skin. if not diagnosed and treated, it may lead to disfigurement of the areas involved and also result in deep invasive infections. | 2013 | 23723483 |
| epidemiological characteristics of malassezia folliculitis and use of the may-grünwald-giemsa stain to diagnose the infection. | various bacterial, fungal, parasitic, and viral pathogens can cause folliculitis, which is often mistakenly treated with antibiotics for months or even years. a laboratory diagnosis is required before therapy can be planned. here, we describe the prevalence and risk factors, as well as the clinical, cytological, and mycological characteristics, of patients with malassezia folliculitis (mf) in adana, turkey. we also report the treatment responses of the mf patients and describe the malassezia spp ... | 2013 | 23706503 |
| in vitro antifungal activity of topical and systemic antifungal drugs against malassezia species. | the strict nutritional requirements of malassezia species make it difficult to test the antifungal susceptibility. treatments of the chronic and recurrent infections associated with malassezia spp. are usually ineffective. the objective of this study was to obtain in vitro susceptibility profile of 76 clinical isolates of malassezia species against 16 antifungal drugs used for topical or systemic treatment. isolates were identified by restriction fragment length polymorphism. minimal inhibitory ... | 2013 | 23496653 |
| the antifungal effect of light emitting diode on malassezia yeasts. | malassezia (m.) species are members of the normal part of the skin flora, but they might induce or be involved with various cutaneous diseases. although the role of malassezia in the pathogenesis of cutaneous diseases is not fully understood, recent studies have shown that decreased density of malassezia led to improvement of these diseases. | 2012 | 22551719 |
| inhibition of malassezia globosa carbonic anhydrase with phenols. | a panel of 22 phenols was investigated as inhibitors of the β-class carbonic anhydrase (cas, ec 4.2.1.1) from the fungal parasite malassezia globosa (mgca), a validated anti-dandruff drug target. the displayed inhibitory activities were compared to the ones previously reported against the off-target widely distributed human (h) isoforms hca i and ii. all tested phenols possessed a better efficacy in inhibiting mgca than the clinically used sulfonamide acetazolamide, with kis in the range of 2.5 ... | 2017 | 28343756 |
| dithiocarbamates effectively inhibit the β-carbonic anhydrase from the dandruff-producing fungus malassezia globosa. | a series of dithiocarbamates (dtcs) was investigated for the inhibition of the β-class carbonic anhydrase (cas, ec 4.2.1.1) from the fungal parasite malassezia globosa, mgca, a validated anti-dandruff drug target. these dtcs incorporate various scaffold, among which those of n,n-dimethylaminoethylenediamine, the aminoalcohols with 3-5 carbon atoms in their molecule, 3-amino-quinuclidine, piperidine, morpholine and piperazine derivatives, as well as phenethylamine and its 4-sulfamoylated derivati ... | 2017 | 28057408 |
| whole genome sequencing analysis of the cutaneous pathogenic yeast malassezia restricta and identification of the major lipase expressed on the scalp of patients with dandruff. | malassezia species are opportunistic pathogenic fungi that are frequently associated with seborrhoeic dermatitis, including dandruff. most malassezia species are lipid dependent, a property that is compensated by breaking down host sebum into fatty acids by lipases. in this study, we aimed to sequence and analyse the whole genome of malassezia restricta kctc 27527, a clinical isolate from a korean patient with severe dandruff, to search for lipase orthologues and identify the lipase that is the ... | 2017 | 27882618 |
| sweat allergy. | for many years, sweat has been recognized as an exacerbation factor in all age groups of atopic dermatitis (ad) and a trigger of cholinergic urticaria (cholu). recently, we reported the improvement of ad symptoms by spray with tannic acid, which suppresses basophil histamine release by semipurified sweat antigens in vitro, and showering that removes antigens in sweat from the skin surface. we finally identified mgl_1304 secreted by malassezia globosa as a major histamine-releasing antigen in hum ... | 2016 | 27584969 |
| simplified enzymatic upgrading of high-acid rice bran oil using ethanol as a novel acyl acceptor. | one of the major challenges in the upgrading of high-acid rice bran oil (rbo) is to efficiently reduce the amount of free fatty acids. here we report a novel method for upgrading high-acid rbo using ethanol as a novel acyl acceptor in combination with a highly selective lipase from malassezia globosa (smg1-f278n). this process enabled an unprecedented deacidification efficiency of up to 99.80% in a short time (6 h); the immobilized smg1-f278n used in deacidification exhibited excellent operation ... | 2016 | 27571030 |
| β-endorphin enhances the phospholipase activity of the dandruff causing fungi malassezia globosa and malassezia restricta. | β-endorphin is known to stimulate phospholipase production by malassezia pachydermatis during canine dermatoses. the role of β-endorphin in malassezia infection in humans is not well studied. the present study compares the influence of β-endorphin on malassezia globosa and malassezia restricta isolated from patients with seborrhoeic dermatitis/dandruff (sd/d) and healthy controls. malassezia isolates (five each of the two species from patients and healthy controls) were grown on modified dixon's ... | 2017 | 27497434 |
| diverse human skin fungal communities in children converge in adulthood. | understanding the skin mycobiome (fungal communities) is important because both commensal and pathogenic fungi can drive cutaneous disease depending on host status and body sites, including the scalp, feet, and groin. interestingly, age may also affect skin fungal infections as certain dermatophytoses (i.e., tinea capitis) are more frequent in children than adults. we previously described the skin mycobiomes in healthy adults, showing lipophilic fungi malassezia predominate in most skin sites. b ... | 2016 | 27476723 |
| lid mobility in lipase smg1 validated using a thiol/disulfide redox potential probe. | most lipases possess a lid domain above the catalytic site that is responsible for their activation. lipase smg1 from malassezia globose cbs 7966 (malassezia globosa lip1), is a mono- and diacylglycerol lipase with an atypical loop-like lid domain. activation of smg1 was proposed to be solely through a gating mechanism involving two residues (f278 and n102). however, through disulfide bond cross-linking of the lid, this study shows that full activation also requires mobility of the lid domain, c ... | 2016 | 27419053 |
| low dna sequence diversity of the intergenic spacer 1 region in the human skin commensal fungi malassezia sympodialis and m. dermatis isolated from patients with malassezia-associated skin diseases and healthy subjects. | as dna sequences of the intergenic spacer (igs) region in the rrna gene show remarkable intraspecies diversity compared with the small subunit, large subunit, and internal transcribed spacer region, the igs region has been used as an epidemiological tool in studies on malassezia globosa and m. restricta, which are responsible for the exacerbation of atopic dermatitis (ad) and seborrheic dermatitis (sd). however, the igs regions of m. sympodialis and m. dermatis obtained from the skin of patients ... | 2016 | 27371104 |
| azole antifungal sensitivity of sterol 14α-demethylase (cyp51) and cyp5218 from malassezia globosa. | malassezia globosa cytochromes p450 cyp51 and cyp5218 are sterol 14α-demethylase (the target of azole antifungals) and a putative fatty acid metabolism protein (and a potential azole drug target), respectively. lanosterol, eburicol and obtusifoliol bound to cyp51 with kd values of 32, 23 and 28 μm, respectively, catalyzing sterol 14α-demethylation with respective turnover numbers of 1.7 min(-1), 5.6 min(-1) and 3.4 min(-1). cyp5218 bound a range of fatty acids with linoleic acid binding stronges ... | 2016 | 27291783 |
| n-nitrosulfonamides: a new chemotype for carbonic anhydrase inhibition. | a series of n(1)-substituted aromatic sulfonamides was obtained by applying a selective sulfonamide nitration synthetic strategy leading to ar-so2nhno2 derivatives which were investigated as carbonic anhydrase (ca, ec 4.2.1.1) inhibitors. two human (h) hca isoforms, the cytosolic hca ii and the transmembrane hca ix, in addition to the fungal enzyme from malassezia globosa, mgca, were included in the study. most of the new compounds reported selectively inhibited hca ix over hca ii and at the sam ... | 2016 | 27290692 |
| evaluation of malassezia and common fungal pathogens in subtypes of chronic rhinosinusitis. | fungal hypersensitivity and fungal microbiome dysbiosis are possible etiologies of chronic rhinosinusitis. the sinus fungal microbiome is not well characterized; novel sinus-associated fungi, including malassezia, have only recently been described. the goals for this study were to verify malassezia as a dominant component of the sinus microbiome, to speciate sinus malassezia, and to compare select fungal species in chronic rhinosinusitis (crs) subtypes with known fungal association to chronic rh ... | 2016 | 27153455 |
| malassezia arunalokei sp. nov., a novel yeast species isolated from seborrheic dermatitis patients and healthy individuals from india. | the majority of species within the genus malassezia are lipophilic yeasts that colonize the skin of warm-blooded animals. two species, malassezia globosa and malassezia restricta, are implicated in the causation of seborrheic dermatitis/dandruff (sd/d). during our survey of sd/d cases, we isolated several species of malassezia and noticed vast variations within a few lipid-dependent species. variations observed in the phenotypic characteristics (colony morphology, absence of catalase activity, g ... | 2016 | 27147721 |
| secreted lipases from malassezia globosa: recombinant expression and determination of their substrate specificities. | malassezia globosa, which is associated with skin conditions such as dandruff and seborrhoeic dermatitis, possesses 13 secreted lipases, but only mglip1, mgmdl2 and mglip2 have been characterized. to understand the substrate preferences of these lipases and by extension their potential role in colonizing human skin, we expressed all 13 predicted secreted lipases in pichia pastoris and evaluated their ability to utilize mono-, di- and triolein substrates. the m. globosa family class 3 lipases wer ... | 2016 | 27130210 |
| carbonic anhydrase activators: activation of the β-carbonic anhydrase from malassezia globosa with amines and amino acids. | the β-carbonic anhydrase (ca, ec 4.2.1.1) from the dandruff producing fungus malassezia globosa, mgca, was investigated for its activation with amines and amino acids. mgca was weakly activated by amino acids such as l-/d-his, l-phe, d-dopa, d-trp, l-/d-tyr and by the amine serotonin (kas of 12.5-29.3μm) but more effectively activated by d-phe, l-dopa, l-trp, histamine, dopamine, pyridyl-alkylamines, and 4-(2-aminoethyl)-morpholine, with kas of 5.82-10.9μm. the best activators were l-adrenaline ... | 2016 | 26856923 |
| a human monoclonal ige antibody that binds to mgl_1304, a major allergen in human sweat, without activation of mast cells and basophils. | mgl_1304, a major allergen in human sweat for patients with atopic dermatitis and/or cholinergic urticaria, is secreted from malassezia globosa on human skin. the amounts of mgl_1304 and ige against mgl_1304 are evaluated by the histamine release test using basophils or mast cells sensitized with serum containing ige against mgl_1304, and enzyme linked sorbent assay (elisa) using mgl_1304 and anti-mgl_1304 antibodies. here, we identified a human monoclonal ige (abs-ige) that binds to the high af ... | 2015 | 26541454 |
| a new procedure for the cloning, expression and purification of the β-carbonic anhydrase from the pathogenic yeast malassezia globosa, an anti-dandruff drug target. | malassezia yeasts are almost exclusively the single eukaryotic members of the fungal flora of the skin. malassezia globosa and malassezia restricta are found on the skin of practically all humans. malassezia globosa is highly implicated in the pathogenesis of dandruff and its genome encodes for only one carbonic anhydrases (cas, ec 4.2.1.1) belonging to the β-class (mgca). it has been indeed demonstrated that in many pathogenic microorganisms, cas are essential for their life cycle and their inh ... | 2016 | 26523349 |
| anion inhibition studies of the dandruff-producing fungus malassezia globosa β-carbonic anhydrase mgca. | the genome of the fungal parasite malassezia globosa, the causative agent of dandruff, contains a single gene annotated as encoding a carbonic anhydrase (cas, ec 4.2.1.1) belonging to the β-class (mgca). in an earlier work (j. med. chem. 2012, 55, 3513) we have validated this enzyme as an anti-dandruff drug target, reporting that sulfonamide inhibitors show in vitro and in vivo effects, in an animal model of malassezia infection. however, few classes of compounds apart the sulfonamides, were inv ... | 2015 | 26459213 |
| comparative genomics including the early-diverging smut fungus ceraceosorus bombacis reveals signatures of parallel evolution within plant and animal pathogens of fungi and oomycetes. | ceraceosorus bombacis is an early-diverging lineage of smut fungi and a pathogen of cotton trees (bombax ceiba). to study the evolutionary genomics of smut fungi in comparison with other fungal and oomycete pathogens, the genome of c. bombacis was sequenced and comparative genomic analyses were performed. the genome of 26.09 mb encodes for 8,024 proteins, of which 576 are putative-secreted effector proteins (pseps). orthology analysis revealed 30 ortholog pseps among six ustilaginomycotina genom ... | 2015 | 26314305 |
| identification and characterization of lipases from malassezia restricta, a causative agent of dandruff. | dandruff, a skin disorder affecting 50% of the world population, is linked with proliferation of lipophilic yeasts of the genus malassezia (particularly malassezia globosa and m. restricta). most malassezia species show a unique lipid dependency and require external lipids for growth. genome mining of the incomplete m. restricta genome led to the identification of eight lipase sequences. sequences representing the class 3 and lip lipase families were used to clone the lipases mrlip1, mrlip2 and ... | 2015 | 26298017 |
| the role of residues 103, 104, and 278 in the activity of smg1 lipase from malassezia globosa: a site-directed mutagenesis study. | the smg1 lipase from malassezia globosa is a newly found mono- and diacylglycerol (dag) lipase that has a unique lid in the loop conformation that differs from the common alpha-helix lid. in the present study, we characterized the contribution of three residues, l103 and f104 in the lid and f278 in the rim of the binding site groove, on the function of smg1 lipase. sitedirected mutagenesis was conducted at these sites, and each of the mutants was expressed in the yeast pichia pastoris, purified, ... | 2015 | 26239010 |
| novel inhibitor against malassezia globosa lip1 (smg1), a potential anti-dandruff target. | compelling evidence have demonstrated the role of lipase activity in the pathogenicity of malassezia globosa toward dandruff and seborrheic dermatitis (d/sd). as a representative secreted lipase from m. globosa cbs 7966, malassezia globosa lip1 (smg1) is considered a potential anti-dandruff target. in this study, homology modeling, docking-based virtual screening and in vitro lipase-based assay were integrated to identify the first hit compound against smg1, with an ic50 of 20 μm against synthet ... | 2015 | 26199121 |