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pseudomonas monteilii sp. nov., isolated from clinical specimens.we propose the name pseudomonas monteilii for a new species of gram-negative, rod-shaped, motile bacteria that were nonhemolytic on blood agar and were isolated from clinical sources. the 10 strains of p. monteilii were incapable of liquefing gelatin. they grew at 10 degrees c but not at 41 degrees c, produced fluorescent pigments, catalase, and cytochrome oxidase, and possessed the arginine dihydrolase system. they were capable of respiratory but not fermentative metabolism. they did not hydrol ...19979226917
isolation of a pseudomonas monteilli strain with a novel phosphotriesterase.a pseudomonas monteilli strain (designated c11) that uses the phosphotriester coroxon as its sole phosphorus source has been isolated. native page and activity staining identified a single isozyme with significant phosphotriesterase activity in the soluble fraction of the cell. this phosphotriesterase could hydrolyse both coumaphos and coroxon. the hydrolysis product of coroxon, diethylphosphate, and the thion analogue, coumaphos, could not serve as phosphorus sources when added to the growth me ...200211786256
siderophore typing, a powerful tool for the identification of fluorescent and nonfluorescent pseudomonads.a total of 301 strains of fluorescent pseudomonads previously characterized by conventional phenotypic and/or genomic taxonomic methods were analyzed through siderotyping, i.e., by the isoelectrophoretic characterization of their main siderophores and pyoverdines and determination of the pyoverdine-mediated iron uptake specificity of the strains. as a general rule, strains within a well-circumscribed taxonomic group, namely the species pseudomonas brassicacearum, pseudomonas fuscovaginae, pseudo ...200212039729
a mycorrhiza helper bacterium enhances ectomycorrhizal and endomycorrhizal symbiosis of australian acacia species.the aims of this study were to test the effects of a mycorrhiza helper bacterium (mhb), pseudomonas monteilii strain hr13 on the mycorrhization of (1) an australian acacia, a. holosericea, by several ectomycorrhizal fungi or one endomycorrhizal fungus glomus intraradices, and (2) several australian acacia species by pisolithus alba strain ir100 under glasshouse conditions. bacterial inoculant hr13 significantly promoted ectomycorrhizal colonization for all the acacia species, from 45.8% ( a. man ...200312682830
genomotyping of pseudomonas putida strains using p. putida kt2440-based high-density dna microarrays: implications for transcriptomics studies.pseudomonas putida kt2440 is the only fully sequenced p. putida strain. thus, for transcriptomics and proteomics studies with other p. putida strains, the p. putida kt2440 genomic database serves as standard reference. the utility of kt2440 whole-genome, high-density oligonucleotide microarrays for transcriptomics studies of other pseudomonas strains was investigated. to this end, microarray hybridizations were performed with genomic dnas of subcultures of p. putida kt2440 (dsm6125), the type st ...200717370070
taxonomic heterogeneity, as shown by siderotyping, of strains primarily identified as pseudomonas putida.one hundred and forty-four fluorescent pseudomonad strains isolated from various environments (soil, water, plant rhizosphere, hospital) and received as pseudomonas putida (83 strains), p. putida biovar a (49 strains), p. putida biovar b (10 strains) and p. putida biovar c (2 strains), were analysed by the pyoverdine-isoelectrofocusing and pyoverdine-mediated iron uptake methods of siderotyping. both methods demonstrated a great diversity among these strains, which could be subdivided into 35 si ...200717978216
effect of osmotic stress on plant growth promoting pseudomonas spp.in this study we isolated and screened drought tolerant pseudomonas isolates from arid and semi arid crop production systems of india. five isolates could tolerate osmotic stress up to -0.73 mpa and possessed multiple pgp properties such as p-solubilization, production of phytohormones (iaa, ga and cytokinin), siderophores, ammonia and hcn however under osmotic stress expression of pgp traits was low compared to non-stressed conditions. the strains were identified as pseudomonas entomophila, pse ...201020700582
differential habitat use and niche partitioning by pseudomonas species in human homes.many species of pseudomonas have the ability to use a variety of resources and habitats, and as a result pseudomonas are often characterized as having broad fundamental niches. we questioned whether actual habitat use by pseudomonas species is equally broad. to do this, we sampled extensively to describe the biogeography of pseudomonas within the human home, which presents a wide variety of habitats for microbes that live in close proximity to humans but are not part of the human flora, and for ...201121503776
imp-13-producing pseudomonas monteilii recovered in a hospital environment. 201121764825
environmental microbiota represents a natural reservoir for dissemination of clinically relevant metallo-beta-lactamases.a total of 10 metallo-β-lactamase-producing isolates of six different species, including brevundimonas diminuta (n = 3), rhizobium radiobacter (n = 2), pseudomonas monteilii (n = 1), pseudomonas aeruginosa (n = 2), ochrobactrum anthropi (n = 1), and enterobacter ludwigii (n = 1), were detected in the sewage water of a hospital. the presence of bla(vim-13) associated with a tn1721-class 1 integron structure was detected in all but one of the isolates (e. ludwigii, which produced vim-2), and in tw ...201121859934
abattoirs as non-hospital source of extended spectrum beta lactamase producers: confirmed by the double disc synergy test and characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry.in this study, the presence of extended spectrum beta lactamase (esbl) producing organisms in abattoirs, a non-hospital community was investigated. the presence of esbl-producing phenotypes was confirmed by the double disc synergy test (ddst). out of the 99 isolates screened for esbl, 28 (28.3%) were confirmed positive. the positive isolates were characterised by using matrix-assisted laser desorption/ionization time of flight mass spectrometry. 50% of the isolates were pseudomonas spp., the res ...201424728403
isolation of vim-2-producing pseudomonas monteilii clinical strains disseminated in a tertiary hospital in northern spain.we describe here the occurrence of blavim-2 in 10 carbapenem-resistant pseudomonas monteilii strains isolated from different clinical samples from patients at the university hospital marqués de valdecilla in northern spain. all the blavim-2-harboring p. monteilii isolates possessed a class 1 integron, with the cassette array [inti1_blavim-2_aac(6')-ib_qaceδ1_sul1]. our results show the emergence of vim-2-producing multidrug-resistant species other than pseudomonas aeruginosa or pseudomonas putid ...201525421471
unusual microorganisms and antimicrobial resistances in a group of syrian migrants: sentinel surveillance data from an asylum seekers centre in italy.three years of civil war in syria have caused death and increase of communicable diseases. the suffering population has been forced to migrate creating a fertile condition for epidemic spread of infection within the refugee camps.201726987764
degradation of norgestrel by bacteria from activated sludge: comparison to progesterone.natural and synthetic progestagens in the environment have become a concern due to their adverse effects on aquatic organisms. laboratory studies were performed to investigate aerobic biodegradation of norgestrel by bacteria from activated sludge in comparison with progesterone, and to identify their degradation products and biotransformation pathways. the degradation of norgestrel followed first order reaction kinetics (t1/2 = 12.5 d), while progesterone followed zero order reaction kinetics (t ...201323952780
plant growth-promotion and biofortification of chickpea and pigeonpea through inoculation of biocontrol potential bacteria, isolated from organic soils.seven strains of bacteria [pseudomonas plecoglossicida sri-156, brevibacterium antiquum sri-158, bacillus altitudinis sri-178, enterobacter ludwigii sri-211, e. ludwigii sri-229, acinetobacter tandoii sri-305 and pseudomonas monteilii sri-360; demonstrated previously for control of charcoal rot disease in sorghum and plant growth-promotion (pgp) in rice] were evaluated for their pgp and biofortification traits in chickpea and pigeonpea under field conditions. when treated on seed, the seven sele ...201627833841
pseudomonas hunanensis sp. nov., isolated from soil subjected to long-term manganese pollution.a gram-negative, polar flagella, rod-shaped bacterium lv (t) was isolated from a soil sample subjected to long-term manganese pollution in hunan province, china. cells grow optimally on luria-bertani agar medium at 30 °c in the presence of 0-5.0 % (w/v) nacl and ph 7-8. 16s rrna gene sequence analysis revealed that strain lv (t) belonged to the genus pseudomonas, with sequence similarity values of 98.6, 98.2, 98.7, and 97.3 % to pseudomonas monteilii bcrc 17520 (t) , pseudomonas putida bcrc 1045 ...201424562759
molecular and in situ characterization of cadmium-resistant diversified extremophilic strains of pseudomonas for their bioremediation potential.cadmium-resistant strains psychrotolerant pseudomonas putida sb32 and alkalophilic pseudomonas monteilli sb35 were originally isolated from the soil of semera mines, palamau, jharkhand, india. further, to unravel the mechanism involved in cadmium resistance, plasmid dna was isolated from the strains and subjected to amplification of the czc gene, which is responsible for the efflux of three metal cations, viz. co, zn and cd, from the cell. furthermore, the amplicon was cloned into pdrive cloning ...201428324433
exacerbation of bronchiectasis by pseudomonas monteilii: a case report.pseudomonas spp are important opportunistic and nosocomial pathogens. one such species is pseudomonas monteilii (p. monteilii). it has been described as an environmental contaminant and potential pathogen. we identified this organism as the causative agent of an exacerbation of bronchiectasis and an environmental contaminant in our hospital on two separate occasions.201728738817
petroleum oil removal by immobilized bacterial cells on polyurethane foam under different temperature conditions.in this work, a mixed biofilm composed by pseudomonas monteilii p26 and gordonia sp. h19 was formed using polyurethane foam (puf) as immobilization support, for crude oil removal from artificial sea water. fresh immobilized cells and immobilized cells that were stored at 4°c for two months before use were assessed. the oil removal assays were carried out at microcosm scale at 4, 15 and 30°c. a viability loss of p. monteilii p26 was observed after the storage. the highest removal value (75%) was ...201728641883
development of a mixed microbial culture for thiocyanate and metal cyanide degradation.the degradation capacity of a mixed culture of agrobacterium tumefaciens suts 1 and pseudomonas monteilii suts 2 for thiocyanate and metal cyanide, in the form of zinc and cadmium, has been determined. the growth of a mixed culture of suts 1 and suts 2 in cyanide complexes and the cyanide removal efficiency of a fixed-film bio-column system were studied. the results showed that the mixed culture of bacteria can survive and grow in broth media containing thiocyanate and metal cyanide complexes wi ...201728664381
draft genome of formaldehyde-degrading strain, pseudomonas monteilii iofa19.the draft genome of pseudomonas monteilii strain iofa19 isolated from deep-sea sediment of the indian ocean was sequenced to gain insights into its efficient metabolic pathway for degrading formaldehyde. the annotated genome of this strain was subjected to comparative analysis with the genomes of existing sequenced p. monteilii strains qm, sb3101, and sb3078. differences in the overall genomic content were observed, and relative genes that were hypothetically involved in the formaldehyde-degradi ...201424798874
complete genome sequences of pseudomonas monteilii sb3078 and sb3101, two benzene-, toluene-, and ethylbenzene-degrading bacteria used for bioaugmentation.pseudomonas monteilii sb3078 and sb3101 are benzene-, toluene-, and ethylbenzene-degrading strains used for bioaugmentation in relation to treatment of wastewater contaminated with petrochemical hydrocarbons. complete genome sequencing of the bioaugmentation strains confirms that they are very closely related (100.0% average nucleotide identity). both strains contain extensive integration of phage elements, with the main difference being insertion of additional phage elements in the sb3078 genom ...201424874689
impact of plant growth promoting pseudomonas monteilii psf84 and pseudomonas plecoglossicida psf610 on metal uptake and production of secondary metabolite (monoterpenes) by rose-scented geranium (pelargonium graveolens cv. bourbon) grown on tannery sludge amended soil.bacterial strains psf84 and psf610 were isolated from tannery sludge polluted soil, jajmau, kanpur, india. 16s rrna gene sequence and phylogenetic analysis confirmed the taxonomic affiliation of psf84 as pseudomonas monteilii and psf610 as pseudomonas plecoglossicida. a greenhouse study was carried out with rose-scented geranium (pelargonium graveolenscv. bourbon) grown in soil treated with tannery sludge in different proportions viz. soil: sludge ratio of 100:0, 25:75, 50:50, 75:25 and 0:100 to ...201425194330
characterization of pseudomonas monteilii ccm 3423 and its physiological potential for biodegradation of selected organic pollutants.pseudomonas monteilii ccm 3423 bacterial strain, deposited at the czech collection of microorganisms, was originally isolated by haľama and augustín (1980) as a bacterium degrading aromatic hydrocarbons and derivates. a detailed study supported by a molecular genetics method of sequence analyses of rrs and rpod genes was used to reclassify the strain, originally stored as 'pseudomonas putida'. the physiological characteristics of the strain are complemented with research in the capacity to utili ...201525543262
survival and activity of individual bioaugmentation strains.successful application of bioaugmentation for enhanced degradation of environmental pollutants is often limited by the lack of methods to monitor the survival and activity of individual bioaugmentation strains. however, recent advancements in sequencing technologies and molecular techniques now allow us to address these limitations. here a complementing set of general applicable molecular methods are presented that provides detailed information on the performance of individual bioaugmentation st ...201525817029
the metagenome of utricularia gibba's traps: into the microbial input to a carnivorous plant.the genome and transcriptome sequences of the aquatic, rootless, and carnivorous plant utricularia gibba l. (lentibulariaceae), were recently determined. traps are necessary for u. gibba because they help the plant to survive in nutrient-deprived environments. the u. gibba's traps (ugt) are specialized structures that have been proposed to selectively filter microbial inhabitants. to determine whether the traps indeed have a microbiome that differs, in composition or abundance, from the microbio ...201626859489
selective ciprofloxacin antibiotic detection by fluorescent siderophore pyoverdin.fluorescent siderophore pyoverdin (pvd) was produced from a soil isolate pseudomonas monteilii strain mkp 213. the pvd was purified near to homogeneity and applied for the fluorescent chemosensing of various antibiotics in aqueous solution (ph=7.0). upon addition of ciprofloxacin, pvd showed new uv-vis absorption bands at 252 and 321nm due to an internal charge transfer mechanism. also, the addition of ciprofloxacin induced a highly selective fluorescence enhancement of pvd with a 13nm blue shif ...201626971273
a novel biosensor for p-nitrophenol based on an aerobic anode microbial fuel cell.p-nitrophenol is one of the most common contaminants in chemical industrial wastewater, and in situ real-time monitoring of pnp cannot be achieved by conventional analytical techniques. here, a two-chamber microbial fuel cell with an aerobic anode chamber was tested as a biosensor for in situ real-time monitoring of pnp. pseudomonas monteilii lzu-3, which was used as the biological recognition element, can form a biofilm on the anode electrode using pnp as a sole substrate. the optimal operation ...201627295573
pseudomonas guariconensis sp. nov., isolated from rhizospheric soil.we isolated a bacterial strain designated pcavu11(t) in the course of a study of phosphate-solubilizing bacteria occurring in rhizospheric soil of vigna unguiculata (l.) walp. in guárico state, venezuela. the 16s rrna gene sequence had 99.2 % sequence similarity with respect to the most closely related species, pseudomonas taiwanensis, and 99.1 % with respect to pseudomonas entomophila, pseudomonas plecoglossicida and pseudomonas monteilii, on the basis of which pcavu11(t) was classified as repr ...201323847284
purification, characterization, and in vitro activity of 2,4-di-tert-butylphenol from pseudomonas monteilii psf84: conformational and molecular docking studies.a novel strain of pseudomonas monteilii, psf84, was isolated from tannery waste soil from jajmau, kanpur, india. 16s rrna gene sequence phylogenetic analysis confirmed the taxonomic affiliation of psf84 as p. monteilii. an antifungal volatile organic compound (voc) active against hyphal growth of fusarium oxysporum (cimap-imi-357464) in vitro was isolated from strain psf84 by using chromatographic techniques. the molecular formula of the antifungal voc was deduced to be c₁₄h₂₂o by ei-ms and 1d a ...201424934765
synergy between glomus fasciculatum and a beneficial pseudomonas in reducing root diseases and improving yield and forskolin content in coleus forskohlii briq. under organic field conditions.root rot and wilt, caused by a complex involving fusarium chlamydosporum (frag. and cif.) and ralstonia solanacearum (smith), are serious diseases affecting the cultivation of coleus forskohlii, a crop with economic potential as a source of the medicinal compound forskolin. the present 2-year field experiments were conducted with two bioinoculants (a native pseudomonas monteilii strain and the exotic arbuscular mycorrhizal (am) fungus glomus fasciculatum) alone and in combination under organic f ...201322648372
microorganisms and heavy metals associated with atmospheric deposition in a congested urban environment of a developing country: sri lanka.the presence of bacteria and heavy metals in atmospheric deposition were investigated in kandy, sri lanka, which is a typical city in the developing world with significant traffic congestion. atmospheric deposition samples were analyzed for al, cr, mn, fe, ni, cu, zn, cd and pb which are heavy metals common to urban environments. al and fe were found in high concentrations due to the presence of natural sources, but may also be re-suspended by vehicular traffic. relatively high concentrations of ...201728185730
[isolation of phenol-degrading bacteria from coking wastewater and their degradation gene].phenol and phenolic compounds are main pollutants in wastewater of coking factories. to identify the bacteria responsible for phenol removal in the activated sludge of a coking factory, we isolated bacteria from the sludge directly or after enrichment. from two samples from the aerobic and anaerobic pools, 28 strains belonging to 28 species of 20 genera were obtained after identification with box-pcr and further 16s rdna sequence analyses. most of them belonged to beta- and gamma-proteobacteria, ...201121528584
pseudomonas taiwanensis sp. nov., isolated from soil.a novel gram-negative, rod-shaped, motile, non-spore-forming bacterial strain, cms(t), isolated from soil was characterized using phenotypic and molecular taxonomic methods. 16s rrna gene sequence analysis revealed that the organism belongs phylogenetically to the genus pseudomonas. pseudomonas monteilii, p. plecoglossicida and p. mosselii were the most closely related species, with 16s rrna gene sequence similarities to the respective type strains of 99.79, 99.73 and 99.59 %. relatively low gyr ...201019854877
effects of organoclays on soil eubacterial community assessed by molecular approaches.the aim of this study was to evaluate the effects of the commercial organoclays, cloisite 30b, nanofil 804 and dellite 26c on soil eubacterial community. an enrichment test was carried out on nutrient broth containing the organoclay and the microorganisms previously isolated from soil. four transfers were made, each after 7 days incubation. the molecular analyses on the eubacterial community were performed before treatment and 7 days after each transfer. dna was extracted, amplified with eubacte ...200919304378
purification and characterization of extracellular lipases from pseudomonas monteilii tku009 by the use of soybeans as the substrate.a lipase-producing bacterium was isolated and identified as pseudomonas monteilii tku009. a lipase (f2) and lipase-like materials (f1) were purified from the culture supernatant of p. monteilii tku009 with soybean powder as the sole carbon/nitrogen source. the molecular mass of f1 and f2 was estimated to be 44 kda by sds-page and gel filtration. the optimum ph, optimum temperature, and ph and thermal stabilities of f2 were 7, 40 degrees c, 8-11, and 50 degrees c; and of f1 were 6, 40 degrees c, ...200918810517
genetic and functional diversity among fluorescent pseudomonads isolated from the rhizosphere of banana.fluorescent pseudomonads from banana rhizospheric soil were isolated and screened for the production of enzymes and hormones such as phosphatase, indole-3-acetic acid (iaa), 1-aminocyclopropane-1-carboxylate (acc) deaminase, protease, and antifungal metabolites. of 95 isolates, 50 (52%) isolates solubilized tri-calcium phosphate (tcp), 63 (66%) isolates produced plant growth hormone iaa, 10 (11%) isolates exhibited acc deaminase, and 23 (24%) isolates produced protease. isolates were screened fo ...200818347847
isolation of bisphenol a-tolerant/degrading pseudomonas monteilii strain n-502.bisphenol a (bpa) is a highly biotoxic compound that kills many microorganisms at a low concentration (1,000 ppm). we isolated bpa-tolerant/degrading pseudomonas monteilii strain n-502 from about 1,000 samples collected from a field, sewage, and pond water. the isolated strain had strong bpa tolerance and high bpa-degrading activity. this strain was able to grow in a minimum medium containing bpa as the sole carbon source. strain n-502 is an aerobic, motile, gram-negative, nonspore-forming, rod- ...200717160346
a brevibacillus choshinensis system that secretes cytoplasmic proteins.brevibacillus choshinensis has previously been shown to be a useful strain for the secretion of heterologous proteins via the sec secretory pathway. this pathway involves the secretion of proteins prior to folding, whereas the alternative tat (twin-arginine translocation) pathway enables pre-folded proteins to be secreted. we have modified the signal peptide of the brevibacillus expression vector pncmo2 to accommodate a sec avoidance signal as well as the twin arginines required for secretion vi ...200415925899
cloning and expression of the phosphotriesterase gene hoca from pseudomonas monteilii c11.the cloning of a gene encoding the novel phosphotriesterase from pseudomonas monteilii c11, which enabled it to use the organophosphate (op) coroxon as its sole phosphorus source, is described. the gene, called hoca (hydrolysis of coroxon) consists of 501 bp and encodes a protein of 19 kda. this protein had no sequence similarity to any proteins in the swiss-prot/genbank databases. when a spectinomycin-resistance cassette was placed in this gene, phosphotriesterase activity was abolished and p. ...200212213915
pseudomonas mosselii sp. nov., a novel species isolated from clinical specimens.twenty-two fluorescent pseudomonad strains of clinical origin received as pseudomonas fluorescens (10 strains), pseudomonasputida (10 strains) and pseudomonas sp. (2 strains), and 33 type strains of the genus pseudomonas were studied by numerical analysis based on 280 phenotypic characters. twelve of the 22 clinical isolates clustered within a specific group, cluster iv. the other strains clustered within groups containing well-characterized fluorescent pseudomonas species or did not cluster. st ...200211931144
genome sequence of a novel indigo-producing strain, pseudomonas monteilii qm.pseudomonas monteilii is a versatile bacterium found in various niches. a newly isolated strain, p. monteilii qm, can effectively produce indigoids from indoles. here we present a 5.76-mb assembly of the p. monteilii genome for the first time. it may provide abundant molecular information for the transformation of aromatics.201222843591
development of a bioreactor for remediation of textile effluent and dye mixture: a plant-bacterial synergistic strategy.the objective of the present work was to develop a plant-bacterial synergistic system for efficient treatment of the textile effluents. decolorization of the dye scarlet rr and a dye mixture was studied under in vitro conditions using glandularia pulchella (sweet) tronc., pseudomonas monteilii ank and their consortium. four reactors viz. soil, bacteria, plant and consortium were developed that were subjected for treatment of textile effluents and dye mixture. under in vitro conditions g. pulchel ...201323245543
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