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sinorhizobium arboris sp. nov. and sinorhizobium kostiense sp. nov., isolated from leguminous trees in sudan and kenya.sds-page of total bacterial proteins was applied to the classification of 25 sudanese and five kenyan strains isolated from the root nodules of acacia senegal and prosopis chilensis. twenty strains were also studied by multilocus enzyme electrophoresis (mlee) and the whole 16s rrna gene was sequenced from two strains representing the two major clusters. these results, together with the previously reported numerical taxonomy analysis, pulsed-field gel electrophoresis studies, dna-dna dot-blot hyb ...199910555313
analysis of cellular fatty acids and phenotypic relationships of agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium species using the sherlock microbial identification system.previous studies have demonstrated that cellular fatty acid analysis is a useful tool for identifying unknown strains of rhizobia and establishing taxonomic relationships between the species. in this study, the fatty acid profiles of over 600 strains belonging to the genera agrobacterium, bradyrhizobium, mesorhizobium, rhizobium and sinorhizobium were evaluated using the gaschromatography-based sherlock microbial identification system (mis). data collected with the mis showed that the three phyl ...200010758890
effect of heat stress on cell activity and cell morphology of the tropical rhizobium, sinorhizobium arboris.the effect of heat stress on the growth, physiological state, cell activity and cell morphology of the tropical sinorhizobium arboris strain hambi 2190 was studied. the cells were chromosomally tagged with the firefly luciferase gene, luc. since the bioluminescence phenotype is dependent on cellular energy reserves it was used as an indicator of the metabolic status of the cell population under various heat conditions. variations in the numbers and lengths of growth phases between individual cul ...200111137606
the genus name ensifer casida 1982 takes priority over sinorhizobium chen et al. 1988, and sinorhizobium morelense wang et al. 2002 is a later synonym of ensifer adhaerens casida 1982. is the combination "sinorhizobium adhaerens" (casida 1982) willems et al. 2003 legitimate? request for an opinion.the synonymy of the genera ensifer and sinorhizobium was recently reported, but it was proposed that the later-named genus, sinorhizobium, take priority in nomenclature. there is no justification in the international code of nomenclature of bacteria (prokaryotes) for this step; ensifer is the correct name of the genus, with ensifer adhaerens as the type species. species previously allocated to sinorhizobium are here proposed as the new combinations ensifer arboris, ensifer fredii, ensifer kostie ...200314657154
acacia senegal and prosopis chilensis-nodulating rhizobia sinorhizobium arboris hambi 2361 and s. kostiense hambi 2362 produce tetra- and pentameric lcos that are n-methylated, o-6-carbamoylated and partially sulfated.sinorhizobium arboris and s. kostiense are rhizobia that nodulate the tropical leguminous trees acacia senegal and prosopis chilensis. the lipochito-oligosaccharidic signalling molecules (lcos) of s. arboris hambi 2361 and s. kostiense hambi 2362 were analyzed by mass spectrometry. the major lcos produced by the strains were shown to be pentameric, acylated with common fatty acids, n-methylated, o-6-carbamoylated and partially sulfated, as are the lcos characterized to date for other acacia-nodu ...200415063192
genetic characterization of fast-growing rhizobia able to nodulate prosopis alba in north spain.prosopis is a mimosaceae legume tree indigenous to south america and not naturalized in europe. in this work 18 rhizobial strains nodulating prosopis alba roots were isolated from a soil in north spain that belong to eight different randomly amplified polymorphic dna groups phylogenetically related to sinorhizobium medicae, sinorhizobium meliloti and rhizobium giardinii according to their intergenic spacer and 16s rrna gene sequences. the nodc genes of isolates close to s. medicae and s. melilot ...200718031342
stable isotope labelling reveals that nacl stress decreases the production of ensifer (sinorhizobium) arboris lipochitooligosaccharide signalling molecules.ensifer (sinorhizobium) arboris is a symbiont of salt-tolerant leguminous trees in the genera acacia and prosopis that are utilized in the prevention of soil erosion and desertification and in phytoremediation of salinized soil. signalling between the plant and the rhizobia is essential for the formation of effective symbiosis that increases the success of reclaiming saline sites. we assessed the effect of salt stress on the growth and the production of lipochitooligosaccharide signalling molecu ...201324256411
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