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emended description of the genus phyllobacterium and description of four novel species associated with plant roots: phyllobacterium bourgognense sp. nov., phyllobacterium ifriqiyense sp. nov., phyllobacterium leguminum sp. nov. and phyllobacterium brassicacearum sp. nov.gram-negative bacteria were isolated from the rhizoplane of brassica napus in france and from root nodules of argyrolobium uniflorum, astragalus algerianus and lathyrus numidicus growing in the infra-arid zone of southern tunisia. based on phylogenetic analysis of the 16s rrna gene sequences, the seven isolates belong to the alphaproteobacteria and are related to phyllobacterium myrsinacearum strains. the isolates formed three clusters; clusters a and c consist of tunisian strains, whereas clust ...200616585703
phylogenetic and symbiotic characterization of rhizobial bacteria nodulating argyrolobium uniflorum in tunisian arid soils.forty-two bacterial isolates from root nodules of argyrolobium uniflorum growing in the arid areas of tunisia were characterized by phenotypic features, rflp, and sequencing of pcr-amplified 16s rrna genes. the isolates were found to be phenotypically diverse. the majority of the isolates tolerated 3% nacl and grew at temperatures up to 40 degrees c. phylogenetically, the new isolates were grouped in the genera sinorhizobium (27), rhizobium (13), and agrobacterium (2). except for the 2 agrobacte ...200818388992
multilocus sequence analysis of root nodule isolates from lotus arabicus (senegal), lotus creticus, argyrolobium uniflorum and medicago sativa (tunisia) and description of ensifer numidicus sp. nov. and ensifer garamanticus sp. nov.nine isolates from argyrolobium uniflorum, lotus creticus , medicago sativa (tunisia) and lotus arabicus (senegal) were analysed by multilocus sequence analysis (mlsa) of five housekeeping genes (reca, atpd, glna, glta and thrc), the 16s rrna gene and the nodulation gene noda. analysis of the individual and concatenated gene sequences demonstrated that the nine new strains constituted three stable, well-supported (bootstrap and gene sequence similarity values) monophyletic clusters, a, b and c, ...201019656922
symbiotic diversity of ensifer meliloti strains recovered from various legume species in tunisia.ensifer meliloti (formerly sinorhizobium meliloti) was first considered as a specific microsymbiont of medicago, melilotus and trigonella. however, strains of e. meliloti were recovered from root nodules of various legume species and their symbiotic status still remains unclear. here, we further investigate the specificity of these strains. a collection of 47 e. meliloti strains isolated in tunisia from root nodules of medicago truncatula, medicago sativa, medicago ciliaris, medicago laciniata, ...200919665858
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